Nicholas C. Gosstola

*** ******** ****** ******, *** York 12054

518-***-****

addtk1@r.postjobfree.com

EDUCATION: Bachelor of Science, May 2018

Major: Biology

The University of Alabama, Tuscaloosa, AL

Bachelor of Science in Chemistry, May 2018

Major: Chemistry

The University of Alabama, Tuscaloosa, AL

GPA: 3.75/4.0 magna cum laude

RELEVANT EXPERIENCE:

Research Associate I, Department of Otolaryngology, University of Miami Miller School of Medicine - October 2018 to Present – Human Genetics Gene Therapy Lab

·Lab focused on using CRISPR/Cas9 as a potential gene therapy for underlining genetic deafness mutations

·Main duties included maintaining stem cell culture and differentiating experiments

·Differentiations included Schwann cells, Inner-Ear Hair Like cells, Otic Progenitors, Trilineage differentiation

·Utilized lab techniques such as RT-PCR/qRT-PCR and gel electrophoresis for gene expression analysis, immunocytochemistry, preparation of samples for sequencing, DNA/RNA extraction

·Trained rotating T32 residents and PhD students on basic lab techniques

·Utilized programs such as UCSC genome browser, sequencher, and imagej

·Other duties included bacterial transformation for plasmid preparation, extraction of fibroblasts from a mouse tail, clonal selection after CRISPR/Cas9 experiment, or PBMC isolations

Research Associate I, Department of Otolaryngology, University of Miami Miller School of Medicine - October 2018 to May 2019 – Mouse Lab

Lab used mice with knock-out/in mutations for various genes related to genetic deafness, Alzheimer’s, or autism

Goal was to understand molecular mechanisms of disease in the animal model

Maintained 11 mouse colonies which included keeping cages within guidelines, organizing cages, record keeping, and genotyping the animals

Other duties involved assisting behavior tests such as ABR and fear conditioning, fixing mice heads for brain and cochlear dissection, or intraperitoneal injections

Biomedical Research Intern at Neural Stem Cell Institute, Rensselaer, NY – May 8th to July 28th, 2017

·Project focused on prevention of age-related Macular Degeneration

·Experimentation involved drug effects on differentiation of retina pigmented epithelial stem cells

·PCR used for genotyping and gene expression assays

·Passaged cells, maintained cell culture, separated DNA with agarose gel electrophoresis, extracted DNA/RNA

Drug Discovery Intern at Southern Research, Birmingham, AL – May 31st to August 5th, 2016

·Worked as medicinal chemist intern for 10 weeks

·Project: 14-3-3 theta protein for Parkinson’s disease

·Took lead drug and developed various organic compounds to make it less of a metabolic liability

·Experiments run involved reactions such as Suzuki coupling, Sn2 substitution, nucleophilic substitutions

·Utilized NMR, HPLC, LCMS for compound analysis

·Submitted 29 drug compounds for cell assay

Celiac disease research with Swiss Webster mice - August 2015 – December 2016

·Use organism to ascertain how gluten fed diets impacted tissues

·Monitor key indicator factors such as; weight, behavior, gastrointestinal symptoms

·Mice were euthanized and dissected for brains/intestines to be sent off for preparation of microscope slides

Diabetes research with Swiss Webster mice – February 2016 – December 2016

·Used organism to ascertain potential relationship between gluten vs. non-gluten diets and diabetes

·Mice monitored for key indicators such as weight and blood glucose levels

·Mice euthanized for experimental conclusions

PUBLICATIONS:

Bademci, Guney, et al. “Long-Range Cis-Regulatory Elements Controlling GDF6 Expression Are Essential for Cochlear Development.” Journal of Clinical Investigation, May 2020, DOI:10.1172/JCI136951

PRESENTATIONS:

Association for Research in Otolaryngology - San Jose, California January 2020

Poster presentation

Differentiation of human iPSCs into Schwann Cells can be used to model Neurofibromatosis Type 2 (NF2) in a 2D in vitro culture system

HONORS/AWARDS:

Spring 2014, Fall 2015, Spring 2016, Fall 2017 Dean’s list member

Fall 2014, Spring 2015, and Spring 2018 Presidents list member

2014 and 2015 SECHC club hockey academic All-American

Golden Key Honor Society member

OTHER:

Alabama Club Lacrosse Team – 2015 to 2018

Alabama Club Hockey Team – 2013 to 2015

VOLUNTEER ACTIVITIES:

Albany Police Athletic League – Summer Mentoring Program 2017

·Met weekly with 2-3 inner-city disadvantaged youth

oTaught and discussed; Goal development, appropriate communications & behavior, respect, education, dress and attitude along with respecting authorities.

Building a Solar Kiln with the University of Alabama Green Building Council – April, 2017

·Solar Kiln is used for storing wet wood to dry without rotting

·Basic woodworking skills were used

·Wood working students and Tuscaloosa arboretum will use the kiln for their own use

Journey through the body – 2010-2012

·Provided education on how the body systems worked and how to keep them healthy

·Worked with children and educating them about preventing the spread of infection

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