Thiruganesh Ramasamy *

THIRUGANESH RAMASAMY, M. Pharm, Ph. D

Phone: +1-412-***-****

addob1@r.postjobfree.com

addob1@r.postjobfree.com

Postdoctoral Associate

University of Pittsburgh Medical Center

Pittsburgh, PA 15261

SUMMARY

I am an enthusiastic and highly motivated scientist with over 12 years of consistent research experience, and I have published over 50 articles. My multidisciplinary research experience includes nanomedicine, formulation & drug delivery, cancer drug discovery, immuno- oncology and dissecting molecular signaling pathway in cancer models. During 2 years of company experience, worked with Contract Manufacturing Organizations (CMOs) to deliver GMP drug product for clinical development.

EDUCATION

Ph.D. in Physical Pharmacy 2012 –2015

Yeungnam University, South Korea.

M. Pharm. in Pharmaceutics 2007 –2009

Dr. MGR Medical University, Tamil Nadu, India

B. Pharm. in Pharmacy 1999 –2003

Dr. MGR Medical University, Tamil Nadu, India

RESEARCH EXPERIENCE

University of Pittsburgh, Pittsburgh, U.S.A. March 2017 - Present Postdoctoral Scholar Heart and Vascular Institute (HVI)-Vascular Medicine Institute (VMI)

• Developing microbubble platform for ultrasound-mediated delivery of STAT3 decoy and STAT3 antisense oligonucleotide (ASO) in head and neck cancers and glioblastoma.

• Developing delivery platform to study the knockdown effect of miR-27a* in squamous carcinoma cells.

• Synthesis of nitrite and nitro-fatty acid-loaded microbubble for treatment in microvascular obstruction using Ischemia/Reperfusion hindlimb model.

• Developing brain endothelial p-selectin targeting strategy to aid the brain imaging in sickle cell (SS) mice.

• Developing new delivery platform to improve the therapeutic effect of small molecules and oligonucleotides/nucleic acids.

• Plan and conduct day-to-day experimental activities relating to assigned research projects. Contribute to proposal writing and experimental data for grants ($1M funding NIH, 2018).

• Troubleshooting skills for problems associated with assays and equipment’s.

• Supervising and training undergraduates, visiting scholars and technicians. Thiruganesh Ramasamy 2

Yeungnam University, Daegu, South Korea February 2012 – January 2017 PhD Student and Postdoctoral Fellow, Institute of Drug Research-College of Pharmacy

• Developed polymer and lipid-based multifunctional nanocarriers as a nanomedicine platform in disease model.

• Developed various metal nanocarriers consisting of gold, silver and selenium as a combinational chemo-photothermal therapy.

• Developed combinational anticancer therapeutics in the treatment of solid tumors.

• Developed delivery systems for the administration of microRNAs and Aptamers as a novel therapeutic agent in cancer malignancies.

• Directly guided and supervised Master students (10) and undergraduates (8). Utah-Inha Drug Delivery System & Advanced Therapeutic Research Center, Korea Research Associate August 2010 – December 2011

• Developed tissue engineering scaffold for tissue repair and regeneration.

• Developed thermosensitive hydrogels for the systemic delivery of proteins and growth factor.

• Generated protocols, SOP and reports on the experimental work and deliver progress presentations at the team and interdepartmental level. Andhra University, India

Research Assistant April 2010 – July 2010

• Design of microspheres dosage formulations for the colon-specific delivery in rheumatoid arthritis.

TECHNICAL SKILLS

• In vivo: Developing mouse xenograft tumor model for various in vivo anticancer study. Hindlimb Ischemia/Reperfusion model in rats, pharmacokinetic study in rats, biodistribution analysis (HPLC), biodistribution imaging, ultrasound treatment. Various type of injection (IP, IV and SC). Histopathology, immunohistochemistry, immunofluorescence, western blotting, ELISA, and RT-PCR.

• In vitro: Cell culture technique, various cancer cell line handling including adherent and non-adherent cells, development of drug-resistant cancer cell lines, BBB transwell model, in vitro flow loop model of microvascular obstruction. Gene silencing with miRNA, cytotoxicity assay (MTT), cellular uptake, apoptosis, clonogenic, wound healing, migration, invasion and, glutathione assay.

• Formulation and Instrumentation: Formulation of polymeric/lipid nanoparticles for drug/gene delivery, solid oral dosage forms, microbubbles, microspheres, liposomes and metal nanoparticles (gold nanorod, nanosphere and selenium), synthesis of block copolymers, fabrication of tissue engineering scaffolds, drug product process Thiruganesh Ramasamy 3

development, physicochemical characterization and pre-formulation activities, lyophilization process. BD FACS Canto II and fortessa (Cell cycle, Annexin V, ROS and JC-1 assay), IncuCyte live cell analysis, confocal laser scanning microscope, HPLC, TEM, SEM, FTIR, DSC, XRD, and QCM-D.

SELECTED PUBLICATIONS

• Ruttala HB, Ramasamy T, Poudel BK et al. Multi-responsive albumin-lonidamine conjugated hybridized gold nanoparticle as a combined photothermal-chemotherapy for synergistic tumor ablation. Acta Biomaterialia 101 (2020) 531-543.

• Ramasamy T, Ruttala HB, Kaliraj K et al. Polypeptide Derivative of Metformin with the Combined Advantage of a Gene Carrier and Anticancer Activity. ACS Biomaterials Science & Engg. 10-201*-****-****.

• Ramasamy T, Ruttala HB, Sundaramoorthy P et al. Multimodal selenium nanoshell-capped Au@ mSiO 2 nanoplatform for NIR-responsive chemo-photothermal therapy against metastatic breast cancer. NPG Asia Materials 10 (2018) 197-216.

• Ramasamy T, Gupta B, Ruttala HB et al. Smart chemistry-based nanosized drug delivery systems for systemic applications: a comprehensive review. Journal of Controlled Release 258

(2017) 226-253.

For full list of publications: https://scholar.google.com/citations?user=r6CT9mcAAAAJ&hl=en PATENTS

Pacella JJ, Chen X, Yu FTH, and Ramasamy T. Functionalized microbubble embodiments for ultrasound-mediated treatment and methods of making and using the same. U.S. Provisional Patent Application No. 62/839,490 filed on April 26, 2019. Ramasamy T, Yong CS and Kim JO. Anticancer pharmaceutical composition comprising doxorubicin and irinotecan and manufacturing method thereof. Korean Intellectual Property Office. Patent Number, 10-1769666. 08/11/2017.

Ruttala HB, Choi HG, Ramasamy T, Kim JO, Yong CS. Layer by layer assembly of albumin conjugate and pharmaceutical composition using the same. Korean Intellectual Property Office. Patent Number, 10-1870025. 06/15/2018

AWARDS

Young Researcher Award, Korean Society of Pharmaceutical Sciences and Technology (KSPST), South Korea, 2014.

Nagai Award for Excellent Research in Cancer, Yeungnam University, South Korea, 2016. PROFESSIONAL SERVICE

Peer Reviewer

Reviewed 250+ articles for journal like Journal of Controlled Release, Cancer Letter, Acta Biomaterialia, Materials and Engineering C, Nanomedicine NBM/Future Medicine, Colloids and Surface B, International Journal of Nanomedicine, European Journal of Pharmacology, and so on. Editor

Consultant Editor – International Journal of Nanomedicine (393 manuscript submission) Academic Editor – Biomedicine (MDPI Journal) (6 manuscript submission)

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