Janet Christine Skerry

* ********** *****

Littlestown, Pa. 17340

(717)- 870-1000

add0bk@r.postjobfree.com

I am a laboratory technician with extensive experience with a wide range of animal models. Experienced in the design, execution and analysis of both prophylactic and therapeutic medical countermeasures against select agent toxins. Currently working as a Laboratory Technician at the United State Army Medical Research Institute for Infectious Diseases (USAMRIID). I received my certification by the American Association of Laboratory Animal Science as an Assistant Laboratory Animal Technician (ALAT) in 2004. Prior to joining USAMRIID I worked as a veterinary technician in small animal, exotic animal, and emergency/trauma medicine. I began my career in research in 2004 at Sobran Inc., located on the National Institutes of Health campus in Bethesda, Maryland. I joined USAMRIID in September 2006.

PROFESSIONAL EXPERIENCE

Kia’aki Technoligies/Goldbelt Raven/True Research Foundation/GDIT

USAMRIID, Fort Detrick, Md

Research Technician 2006- present

Trained in Good Laboratory Practices (GLP). Performed a botulism toxoid vaccine study to assess the potency and stability of the vaccine in Guinea pig model

Managing containment suite Managed an enhanced Biosafety 2 Level (BSL-2) containment suite licensed for the use of Biological Select Agents and Toxins (BSAT).

Writing reports What reports, how many, technical detail?Wrote quarterly/yearly technical reports for sponsors.

Overseeing large Botulinum Collection Supervised the physical inventory and electronic database of over 300 individual strains of Clostridium kbotulinumbotulinum bacterial freezer stocks, spores and genomic DNA as well as a substantial inventory of native toxins. Worked with the Centers for Disease Control and Prevention (CDC) to perform semiannual inspections to ensure federal regulatory compliance.

Injecting mice Don’t just say “injected mice”. Instead say something like, “Conducted small and mid-scale (10 to 400 mouse) vaccine potency, stability and efficacy studies” as well as toxin titration studies. and then discuss the techniques you used. Include toxin challenges and at some point, the toxin titrations.

1.Intramuscular

2.Intraperitoneal

3.Intravenous

4.Subcutaneous

Retro orbital bleeds of mice

Sub mandibular bleeds of mice

Cell culture You need to be more specific. What type of cells, how were they split and why, what study did it support? Etc.

1.Growing of cells

2.Splitting cells

3.Plating cells

4.Processing cells

5.Cracking cells

Protein gels Give more detail. “Experienced with standard polyacrylamide gel protein electrophoresis techniques including staining, Western blotting, protein analysis and documentation methods.

Western blots

OD readings

Toxin production

1.Preparation of media

2.Plating of toxin

3.Inoculation of media

4.Precipitation of toxin

5.Filtering/purification of toxin

Elisa testing

RNA extraction

FPLC (Bio-Rad)

BCA Assay

ALISSA testing

1.Seize X bead preparation

2.Fluorescence testing

Creating protocols

Biacore machine

PCR/RT PCR

1.Sample preparation

2.Thermocycler

3.ElectropherisesElectrophoreses

Supervisor: Robert Webb

Gene Logic Inc. 2005-2006

Gaithersburg, Md

Veterinary Technician

Assisting veterinarian with physical exams of NHP, canines and mini pigs

Performing routine medical treatments on NHP, canines and mini pigs

1.nail trims

2.vaccines

3.fecal checks/worming

4.TB tests

5.ear cleaning

Assisting veterinarian with surgeries of NHP and canines

1.Telemetry units

2.VAP units

Perform all medical treatments for sick or injured animals

Create and update all medical records

Assist technicians with study procedures

Work under GLP conditions

Attend and participate in PI protocol meetings

Catch and restrain (awake) NHP, canines and mini pigs

Blood draws from NHP, canines and mini pigs

Organize and implement enrichment program for

NHP, canines and mini pigs

Supervisor: Erin Hanson

Sobran Inc. 2004 to 2005

National Institute of Health

Bethesda, Md

Veterinary Technician II

Performing routine medical treatments on mice, rats, guinea pigs, rabbits and ground squirrels

Managing breeding colonies of mice

Examining animals and filling out of animal health reports.

Entrance and routine exams on animals

Weaning, ear tagging and tail snipping of mice

Ear tagging of rats and guinea pigs

Performing technical requests for investigators

1.IP injections of mice and rats, guinea pigs, rabbits and ground squirrels

2.Im injections of mice and rats, guinea pigs, rabbits and ground squirrels

3.Intranasal inoculations of rats

Blood draws on rabbits

1.Ear bleeds

2.Cephalic veins

3.Femoral

4.Cardiac

Drawing blood from rats and mice

1.Tail veins

2.Mandibular

Survival blood draws form the vena cava of guinea pigs

Humane euthanasia of animals

Tattooing of rabbits and mouse tails

Assisting facility veterinarian

Record keeping

Lead technician duties when the lead technician is out

1.Communicating with PI

2.Over-seeing/scheduling technical staff

3.processing animal health reports

4.completing numerous charts and computer work

a.Word

b.Excel

Supervisor: Laura Allen

Westminster Emergency, Trauma Center 2001 to 2004

269 Main Street

Westminster, Md. 21157

Emergency, Trauma Technician

All aspects of a hospital technician

Assisting with emergency care of patients

Stabilizing critically injured or sick patients

Assisting with emergency surgeries

Preparing and maintaining surgery suite and equipment

1.Cleaning surgical equipment

2.Autoclaving surgical packs

Answering phones and helping clients with health questions

Worked independently night shift

1.Monitoring patients

2.Treating patients

Laboratory work

1.CBC

2.Chemistry

3.Electrolytes

4.Glucose

5.Fecal

6.Urinalysis

Assisted Doctors with exams and treatments

Placed IV catheters and central lines

Monitor fluid administration

Took radiographs

Intubation of patients

Monitor anesthesia

Supervisor: Dr. Robin Smith

Valley Animal, Avian & Exotic Hospital 2000 to 2001

9125 Reisterstown Road

Owings Mills, Md.

Animal Technician

Supervisor: Dr. Bonnie Miller

Preston Country Club For Pets 1995 to 2000 7363 Old Columbia Road

Columbia, Md.

Assistant Kennel Manager

Supervisor: Leona Imonti

North Rockville Animal Hospital 1994 to 1995

East Gude Drive

Rockville, Md.

Surgery Technician

Supervisor: Jerome Albright

Patuxent Valley Animal Hospital 1987 to 1994

Scaggsville Road

Laurel, Md.

Chief Technician

Supervisor: Nancy Haller

Glen Burnie Animal Hospital 1983 to 1987

Crain Highway

Glen Burnie, Md.

Hospital Technician

Supervisor: Dr. Karen Myers

Beltsville Animal Hospital 1979 to 1983

Beltsville, Md

Veterinary assistant

Supervisor: Dr. Brown

EDUCATION/ SPECIAL STUDIES

Graduated from Laurel Senior High School, Laurel Md.

Attended many Baltimore and DC Academy meetings.

Attended the Eastern States Veterinary Conference in 1993.

Attended several Hills Pet Food and Science Diet seminars.

Attended the Maryland Veterinary Association seminars in 1992.

Attended the Tech Expo in Baltimore, Md. Each year from 1990 to 1994.

Attended several other seminars on various topics such as exotic animal care, nutrition, wound care and skin disorders.

Attended the Nash Academy of Animal Arts for a special cat grooming certification.

Completed Hills Small Animal Dietician Program, Veterinary Nutritional Counselor program and Hills Health Care Connection.

Obtained a bachelors’ degree in Veterinary Medicine from Belford University

Certified ALAT through AALAS

Attended many IBRCC meetings

SPECIAL INFORMATION

I have SSBI security clearance.

I have passed security for the “personal reliability program” through USAMRIID

I am currently enrolled with SIP and am vaccinated against Botulinum

Publications

Posters;

Brown JL, Skerry JC, Smith TJ, Wright PM, and RP Webb. May 2-3 2017. Comparison of Immunogenicity and Protection Following Vaccination with ciBoNT/A HP Using Intramuscular and Intradermal Injection Techniques. The 2017 NCI Annual Spring Research Festival, Fredrick, MD, May 2-3 2017.

Wright PM, Smith LA, Smith TJ, Brown JL, Skerry JC, Guernieri RL, and RP Webb. Production and Immunological Assessment of Recombinant, Catalytically Inactive Clostridium botulinum Neurotoxin Holoproteins as Potential Vaccine Candidates. Presented at the 17th annual NCI Annual Spring Research Festival, Fort Detrick, Fredrick, MD, May 2-3 2017.

PRODUCTION AND IMMUNOLOGICAL ASSESSMENT OF RECOMBINANT, CATALYTICALLY INACTIVE CLOSTRIDIUM BOTULINUM NEUROTOXIN HOLOPROTEINS AS POTENTIAL VACCINE CANDIDATES

Leonard A. Smith1*, Robert P. Webb2, Theresa J. Smith2, Patrick, M. Wright2, Rebecca L. Guernieri2, Jennifer L. Brown2, and Janet C. Skerry2

THE EFFECTIVENESS OF RECOMBINANT HUMAN ANTI-BOTULINUM NEUROTOXIN ANTIBODIES WHEN CHALLENGED WITH MULTIPLE TOXIN SUBTYPES USING MOUSE NEUTRALIZATION ASSAYS

T.J. Smith1, J.L. Brown1, J.B. Skerry1, J.D. Marks2, M. Tomic3, L.A. Smith4

COMPARISON OF IMMUNOGENICITY AND PROTECTION FOLLOWING VACCINATION WITH CIBONT/A HP USING INTRAMUSCULAR AND INTRADERMAL INJECTION TECHNIQUES

J. L. Brown, J. C. Skerry, T. J. Smith, P. M. Wright, R. P. Webb

Potency and Stability of rBoNT/A, B, C, E and F (Hc) Vaccines in Monovalent and Pentavalent Formulations

Janet Skerry1, Theresa J. Smith1, Sarah Norris1, Jackie Campos2, Rajiv Nayar2, Ian Henderson3, Leonard A. Smith1

Detection And Identification Of Botulinum Neurotoxin A-G Using Real-Time PCR

Janet C. Skerry1, Brenna J. Hill2, Daniel C. Douek2, Theresa J. Smith3

Engineering A Femtomolar Affinity Human Monoclonal Antibody

Cross Reactive To All Seven Subtypes Of BoNT/F

Yongfeng Fan1, Jianlong Lou 1, Consuelo Garcia1, Wenwu Zhai 1, Weihua Wen1, Fraser Conrad1, Jennifer Brown 2, Janet C. Skerry2, Theresa J. Smith2, Leonard A. Smith3 and James D. Marks1Of

BA Single Biepitopic Antibody (BeAb) Recapitulates the Potency of a Pair of Monoclonal Antibodies In Neutralization of Botulinum Neurotoxin Serotype A

Jianlong Lou, 1*, Weihua Wen 1, Fraser Conrad1, Qi Meng1, Jianbo Dong 1, Zhengda Sun1, Consuelo Garcia1, Luisa Cheng2, Jennifer Brown3, Janet C. Skerry3, Theresa J. Smith3, Leonard A. Smith4 and James D. Marks1, Neurotoxins A – G Genes Using Real-Time PCR

Monoclonal Antibodies Against Botulinum Neurotoxin Serotype F

Yongfeng Fan, Jianlong Lou, Consuelo Garcia, Wenwu Zhai, Jianbo Dong, Weihua Wen, Fraser Conrad, Jennifer Brown, Janet Skerry, Theresa J. Smith, Leonard A. Smith and James D. Marks

Papers;

Webb RP, Wright PM, Brown JL, Skerry JC, Guernieri RL, Smith, TJ, Stawicki C and Smith LA. 2020. Potency and Stability of a Trivalent, Catalytically Inactive Vaccine Against Botulinum Neurotoxin Serotypes C, E and F (triCEF). Toxicon. 2020 Mar; 176:67-76. doi: 10.1016

Universal and Specific Quantitative Detection of Botulinum Neurotoxin Genes

Brenna J. Hill1, Janet C. Skerry2, Theresa J. Smith2, Stephen S. Arnon3, Daniel C. Douek1

1Human Immunology Section, Vaccine Research Center, National Institutes of Allergy and Infectious Diseases, National Institutes of Health, Bethesda, MD 20892

2Integrated Toxicology Division, USAMRIID, Fort Detrick, MD 21702

3Infant Botulism Treatment and Prevention Program, California Department of Public Health, Richmond, CA 94804

HUMAN MONOCLONAL ANTIBODIES BINDING BOTULINUM NEUROTOXIN TYPES C, D, AND MOSAIC NEUROTOXINS C-D AND D-C Garcia C1, Yan S1, Geren I1, Lou J1, Wen W1, Conrad F1, Smith TJ2, Brown J3, Skerry J3, Smith LA2, Marks JD1

1Department of Anesthesia, University of California, San Francisco, San Francisco, CA94110

2U. S. Army Medical Research and Materiel Command, Fort Detrick, MD 21702.

3 Ke’aki Technologies LLC and Integrated Toxinology Division, United States Army Medical Institute of Infectious Disease, Fort Detrick, MD 21702.

Separation of Betti Reaction Product Enantiomers: Absolute Configuration and Inhibition of Botulinum Toxin John H. Cardellina II,1 Rebecca C. Vieira,1 Vanessa Eccard,1 Janet Skerry,1 Vicki Montgomery,1 Yvette Campbell,1 Virginia Roxas-Duncan,1 William Leister,2 Christopher LeClair,2 David J. Maloney,2 Daniele Padula,3 Gennaro Pescitelli,3 Ilja Khavrutskii,4

Xin Hu,4 Anders Wallqvist,4 and Leonard A. Smith5

The Effectiveness of Recombinant Human Anti-botulinum Neurotoxin Monoclonal Antibodies Against Heterologous Toxin Subtypes

TJ Smith, JL Brown, JC Skerry, JD Marks, M Tomic, LA Smith

Recombinant Botulinum Neurotoxin Hc Subunit (BoNT Hc) and Catalytically Inactive Clostridium botulinum Holoproteins (ciBoNT HPs) as Vaccine Candidates for the Prevention of Botulism.

Robert P Webb, Theresa J Smith, Leonard A Smith, Patrick M Wright, Rebecca L Guernieri, Jennifer L Brown, Janet C Skerry

A Three Monoclonal Antibody Combination Potently Neutralizes Multiple Botulinum Neurotoxin Serotype E Subtypes (In Press)

Consuelo Garcia-Rodriguez 1, Ali Razai 1,2, Isin N. Geren 1,3, Jianlong Lou 1, Fraser Conrad 1, Wei-Hua Wen 1, Shauna Farr-Jones 1, Theresa J. Smith 4, Jennifer L. Brown 5, Janet C. Skerry 5, Leonard A. Smith 6, and James D. Marks 1,*

REFERENCES

WORK

Dr Gary Kubala veterinarian 717-***-****

Vickie Montgomery Research microbiologist 301-***-****

Dr. Robert Webb Microbiologist 301-***-****

PERSONAL

Nancy Haller (410) 955 – 6256

Megan Dillinger (443) 821 - 1218

Lindsay Goodpaster (717) 465 - 1162

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