Manager Plant

*

Castro Wattad

Baqa El garbia-Jatt.

P.o.Box **8

30091.

Cellular. 050-*******

adcqvn@r.postjobfree.com

Employment.

****-**** ****** ******** * member of the Israeli BAR. 2006-2009 The Technological Collage Alqasemi Baqa- Elagarbia Lecturer, and graduation project manager, Biotechnology, and Food Science Dep.

2006-2007 Ministry of justice The Public Defense.

Trainee in law

2001-2004, Hy-Laboratories, Rehovot, Manager Molecular Biology Division.

Development, Contract formulation and Establishment of new representations, Development of new products, Molecular diagnostic of Bacteria, Application of Molecular Biology in monitoring of the quality water and beverage in process.

2000-2001, Sigma-Aldrich, Rehovot, Israel.

Diagnostics Country Manager, Export manager, Medical Account Manager.

Responsible of all the unit acivilities.

Planning and executing annual marketing plans including participation at conferences.

Development of new product for the international market. 1996-1999 Sigma-Aldrich Israel, Har Hotzviem, Jerusalem, Israel Manager, Research and Development, Biotechnology.

Patent examination editing and applications.

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Strategic planning of department activities.

Leading the development of new product, via fermentation, and recombinant bacteria cell culture,

Scale up from R&D to production.

Down stream processing.

Quality control protocols, MMF and SOP approval. Vast experience in all major aspect of Molecular Biology, Cell Culture and Recombinant Technology.

1994-1996, University of California Riverside, USA. Post Doctoral Training under the supervision of the late Prof. Noel Keen. Support by BARD postdoctoral fellowship Grant.

Site directed mutagenesis of virulence factors.

Cloning and expression of fungal virulence and pathogenicity factors.

Natural inhibitors of virulence and pathogenicity factors of Colletotrichum gloeosporioides.

Development of maceration system for the selection of resistant Arabidopsis mutants.

Educations

2003-2006, Ramt Gan Collage of Law, Israel.

LL.B Israel.

1990-1996, PhD, The Hebrew University of Jerusalem, Rehovot, Israel. Faculty of Agriculture Rehovot, Microbiology Department. Thesis titled, The involvement of Pectate lyase on the pathogenicity of Colletotrichum, gloeosporioides on avocado fruits.

Conducted at the department of fresh produce at the ARO, Volcani Center under the supervision of Prof. Dov Prusky andprof Amos Dinoor. 1987-1990, M.sc, The Hebrew University of Jerusalem, Rehovot, Israel. 3

Department of Botany.

Thesis on the Interrelation between respiration and Carbon metabolism at symbiotic algae Anabaena Azollea.

Thesis conducted under the supervision of Prof Elisha Tel Or. Vast experience in utilization of water plants for the removal of heavy metals from water sources.

1982-1985, B.sc, The Hebrew University of Jerusalem, Rehovot, Israel. Department of Microbiology and Plant Disease.

Skills and expertise:

Molecular biology )

Recombinant construction.

Protein over expression in bacterial, yeast systems. ( Site directed mutagenesis.

Sequencing.

Primer design. . .

GENETIC MANOPULATION OF STREPTOMYCESFUNGI / /

BACTERIAL AND CELL CULTURETRANSFORMATION AND INFECTION BIOTECHNOLOGY

STRAIN HUNTING AND MAINTANING

Cloning genomic and cDa library construction and screening.

NB. SB SDSPAGE.

ELECTROFOUCOSING.

SPECTROSCOPY MICROSCOPY.

Column chromatography

HPLC

ELECTRON MICROSCOPY AND MICROTOM OPERATION

Strain selection and preservation while maintaining productivity. Fermentation and down stream processing.

Analytical methodology development.

MMF and SOP formulation .

Large scale upgrading up to 1000liter fermenter and downstream processing KNOWLADGE TRANSFER TO PRODUCTION LINE .

Culture media optimization and screening. in line Qs and Qa maintaining documentation for patents and PCT/ /

COMPUTER

ALL OFICE APPLICATION.

PATENT SERVERS.

STN .

LANGUAGES.

ARABIC.

HEBREW

ENGLISH

DUTH) HOLLAND/

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POTEGASE(MEXICO) .

POST REHABILITATION OF STROKE2015.

MINOR MOTORIC DISABILITY

Require sponsor ship for positions at the eu,and north america 5

.Relevant Publications

1.Wattad Castro; Dinoor Amos and Prusky Dov (1994). Purification of Pectate lyase produced by Colletotrichum gloeosporioides and its inhibition by Epicatechin: A possible Factor involved in the Resistance to unripe Fruits to Antracnose. Molecular Plant-Microbe Interactions 7 (2) 293-297. 2.Wattad, Castro; Freeman Stanley; Dinoor Amos; Prusky Dov (1995). A non-pathogenic mutant of Colletotrichum magna is deficient in extra cellular secretion of Pectate lyase. Molecular Plant-Microbe Interactions 8 (4): 621- 626.

3.Prusky, D., Wattad, C. and Kobiler, I. (1996) Effect of ethylene on the activation of quiescent infections of Colletotrichum Gloeosporioides in avocado fruits. Molecular Plant Microbe Interactions, 9; 864-868. 4.Wattad, Castro; Kobiler David; Dinoor Amos and Prusky Dov (1997) Pectate lyase of Colletotrichum gloeosporioides attacking avocado fruits: cDNA cloning and involvement in pathogenicity. Physiological and Molecular plant pathology, 50, 197-212.

5. Castro Wattad; Marcia Ben shoshan and Michal Cahlipha (1997) Conversation of Mevenolenic acid produced by Aspergillus sp. to Mevenolin. Biotechnology R&D Sigma Aldrich- Israel Har Hotsvim site, Inter-company confident protocols

6.Castro Wattad; Marcia Ben shoshan and Michal Cahlipha (1997). Nikkomycin Z from Streptomyces tendae, a chitin synthetase inhibitor Characterization, Purification, Large scale production and downstream processing . . Biotechnology R&D Sigma Aldrich- Israel Har Hotsvim site, Inter-company confident protocols.

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7. Castro Wattad; Marcia Ben shoshan and Michal Cahlipha( 1997) Radicicol from Diheterospora chlamydosporia,Antifungal macro-lactone antibiotic that inhibits protein tyrosine kinase and induces the differentiation of HL-60 cells into macrophages, Purification, Large scale Production and Downstream Processing . Biotechnology R&D Sigma Aldrich- Israel Har Hotsvim site, Inter-company confident protocols.

8.Castro Wattad ( 1998) Improving compatibility and stability of competent E.coli cells for plasmid transformations. Biotechnology R&D Sigma Aldrich- Israel Har Hotsvim site, Inter-company confident protocols. 9.Castro Wattad (1998) Lyophilized E.Coli competent cells, a new approach in Molecular Biology applications. ( 1999). Biotechnology R&D Sigma Aldrich- Israel Har Hotsvim site, Inter-company confident protocols. 10.Castro Wattad and Michal Cahlipha (1998) Geldnanamycin from Streptomyces hygroscopicus a benzoquinone ansamysen anti-tumour antibiotic. Characterization, Purification, Large scale production and downstream processing. Biotechnology R&D Sigma Aldrich- Israel Har Hotsvim site. Inter-company Confident protocols

11. Castro Wattad; Marcia Ben Shoshan and Michal Cahlipha, (1998). Mevenolin from Aspergillus sp. Cholesterol lowering drug and competitive inhibitor of HMG-CoA reductase. Purification, Large scale production and downstream processing. Biotechnology R&D Sigma Aldrich- Israel Har Hotsvim site, Inter-company confident protocols.

12. Castro Wattad; Marcia Ben Shoshan and Cahlipha Michal ( 1999) Trichostatin a. Produced by Streptomyces sp. A histone deacetylase inhibitor, characterization purification, large scale production and downstream processing). Biotechnology R&D Sigma Aldrich- Israel Har Hotsvim site. Inter-company Confident protocols.

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13. Castro Wattad and Michal Cahlipha,(1999) Management, Long term preservation while maintaining viability and productivity of large scale industrial strains Collections ( Fungal, Bacterial and Streptomyces). Biotechnology R&D Sigma Aldrich- Israel Har Hotsvim site. Inter-company Confident protocols.

14.Castro Wattad 1999. Genetic modification of Streptomyces caespitosus. Utilizing plasmid mediated insertation of antibiotic markers for the over production of Mitomycin c, Inhibitor of DNA synthesis, nuclear division, and cancer cells. Biotechnology R&D Sigma Aldrich- Israel Har Hotsvim site. Inter- company Confident protocols.

15. Barnea, Efrat; ; Asscher, Yael; ; Wattad, Castro; Process for producing freeze dried competent cells and use thereof in cloning. United States Patent Application 200********.

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