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Associate Scientist / Immunology / BioAnalytics

Location:
United States
Posted:
March 20, 2020

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Resume:

MARIE A. MEDYNETS

Terra Alta, WV cell: 304-***-**** adcdrr@r.postjobfree.com

Highly experienced professional seeking career advancement in biotechnology industry

SUMMARY OF QUALIFICATIONS

10+ years practical experience in the field of biotechnology

Bachelor’s degree in Biological Engineering

Human tissue engineering and analytical assay development work experience

Excellent analytical thinking, interpretive, and problem-solving skills

Strong ability to work both independently and in a team

AREAS OF EXPERTISE

Cell Culture Techniques Molecular Diagnostics Tools Confocal Microscopy Imaging

Analytical Assay Development Tissue Engineering Techniques Clinical Sample Processing

Pharmacokinetic Data Analysis Laboratory Management Histology Procedures

Cell based functional assays Protein and genomic material processing and characterization

CAREER TIMELINE

Associate Scientist I Aug. 2018- Current

Meso Scale Diagnostics, LLC. Gaithersburg, MD

Independently designs and completes high throughput ELISA-based immunoassay validation projects

Completes Phase 0 stage of target-specific assay development

Performs high-throughput functional testing of prototype assay kit’s critical reagents

Applies protein purification methods to generate high quality IgG material from animal sera

Analyzes and interprets experimental data and independently plans further experiments

Delivers detailed oral and written progress reports in support of analytical bioassay kit development

Maintains and updates the online database for each project status and timeline within the department

Staff Biologist Nov.2011- Aug. 2018

National Institutes of Health / NINDS / Kelly Services Bethesda, MD

Managed the laboratory of Neuronal Differentiation Unit of Translational Neuroscience Division

Mentored and supervised new hires and interns in performing various laboratory functions

Optimized immunoassay-based screenings of clinical whole blood, sera, and CSF samples

Applied MACS and FACS technologies to isolate hematopoietic cell subtypes from human PBMCs

Designed functional cell based assays with human T lymphocytes and neuronal cells

Performed mammalian/bacterial genome isolation, purification, and characterization

Performed qRT-PCR, immunoblotting, karyotyping, electrophysiology, and cell viability/toxicity assays

Induced pluripotency in somatic cells by procedures such as viral transduction and transfection

Contributed pluripotent stem cell lines and their functional neuronal derivatives to patients’ cell bank

Handled processing of clinical samples in compliance with FDA’s GLP guidelines

Generated 3D cultures of human iPSCs and their derivatives, such as neural organoids

Researched novel techniques for in vitro tissue engineering, such as CRISPR-based gene editing

Applied ICC and confocal microscopy to generate publishable high-resolution imaging data

Created SOPs for high-resolution confocal microscopy imaging procedures

Supervised interns and teaches laboratory techniques to intradepartmental collaborators

Co-authored research manuscripts

Inventoried and restocked unit’s supplies and equipment

Histologist Nov.2009 – Nov. 2011

National Institutes of Health / NIMH / Kelly Services Poolesville, MD

Optimized IHC techniques for visualization of specific neuronal circuits in transgenic mouse CNS

Generated imaging specimen for the Gene Expression Nervous System Atlas project

Helped develop a novel gene-based search system for GENSAT database

Biochemical pharmacology laboratory technician June 2007 – Nov. 2009

Walter Reed Army Institute of Research / Geneva Foundation Silver Spring, MD

Contributed quantitative data to pharmacokinetic properties of oxime therapeutics

Administered SC and IV injections to laboratory animals in strict compliance with SOPs

Euthanized laboratory rodents and collected blood and organ samples

Researched techniques for trans-dermal delivery of cholinesterase reactivating substances

Presented progress reports at departmental meetings

Research Technician Sep. 2006 – May 2007

United States Dept. of Agriculture / ANRI / EMBUL Beltsville, MD

Generated quantitative data for mechanical properties of biopolymers by tensile strength testing

Ran HPLC for organic fiber characterization

Manufactured biodegradable polymers from poultry feathers by operating extruder equipment

EDUCATION

Bachelor of Science: Biological Resources Engineering Sep. 2002 - May 2006

University of Maryland College Park, MD

3.5 GPA

Society of Women Engineers Membership

Phi Beta Kappa Membership

INTERNSHIPS

International Exchange Internship, Biomedical Engineering Department Dec. 2004 – May 2005

Tampere University of Technology, Tampere, Finland

Project: EEG Headset Design

Participated in teamwork design of ergonomic and user-friendly encephalography electrodes

Monitored brain wave activity and analyzed EEG data

Internship, Environmental Engineering Department May 2005 – Sept. 2005

University of Maryland, College Park, MD

Project: Ground Water Bioremediation

Utilized GLPs and sterile techniques to maintain and proliferate active bacterial cell cultures

Prepared pH balancing buffers and other aqueous solutions

LABORATORY SKILLS

Translational Neuroscience Applications

In vitro induction of pluripotency and differentiation into later developmental stages of

neurodegenerative disease patients’ somatic cells

Electrophysiology functional analysis for patients’ clinical samples-derived neuronal cell cultures

Hematopoietic progenitor cell enrichment from neurodegenerative disease patients’ whole blood

Karyotype cytogenetics analysis of established human induced pluripotent stem cell lines

Molecular Diagnostics/Analytical Techniques

RT-PCR, qPCR, and ddPCR-based analysis of human stem cell developmental markers

Isolation, cloning, and sequencing of genes of interest from human tissues

Northern Blots method to detect overexpression of disease-related genes in human cell lineages

Genetic Engineering

CRISPR/Cas9 gene editing

RNA silencing

Nucleofection

Analytical Immunology

Western Blots analysis for both endogenous and expressed retroviral proteins in human tissues

Immunocytochemistry analysis for human cell lines

Histopathology of frozen mouse brain tissue sections

Flow Cytometry-based analysis of human patients’ Hematopoietic Progenitor Cells

ELISA assays for antigen detection in neurodegenerative disease patients’ sera

Biochemistry

Seahorse XF Mitochondrial respiration assay of pathological neuronal cells

Clinical pharmacokinetics and pharmacodynamics assays of cholinesterase therapeutics in

animal models for acute organophosphorus agent poisoning

UV/IR Spectroscopy-based GranzymeB-induced cytotoxicity assays

HPLC analysis of biomaterials

Laboratory Animal Techniques

Injections, and brain tissue collection from rodents

Optical Microscopy

Laser confocal imaging for sub-cellular structures visualization

Multichannel fluorescent and bright field microscopy imaging

PUBLICATIONS

Wang T, Medynets M, Li W, Tyagi R, Sampson K, Malik N, Steiner J, Hadegan A, Kowalak J, Johnson KR, Nath A. (2018). Critical Role of Human Endogenous Retrovirus-K in Stem Cell Function and Neuronal Differentiation. Unpublished manuscript. Section of Infections of the Nervous System, National Institute of Neurological Disorders and Stroke, NIH, Bethesda, MD.

Choi E, Medynets MA, Xu Y, Monaco MCG, Major EO, Wang T, Nath A. (2018). Activated T cells induce proliferation of oligodendrocyte progenitor cells via release of vascular endothelial cell growth factor-A. Unpublished manuscript. Section of Infections of the Nervous System, National Institute of Neurological Disorders and Stroke, NIH, Bethesda, MD.

Lee PR, Johnson TP, Gnanapavan S, Giovannoni G, Wang T, Steiner JP, Medynets MA, Vaal MJ, Gartner V, Nath A. (2017). Protease-activated receptor-1 activation by granzyme B causes neurotoxicity that is augmented by interleukin-1β. Journal of Neuroinflammation. 14(131).

Wang T, Choi E, Monaco MCG, Major EO, Medynets MA, Nath A. (2015). Direct Induction of Human Neural Stem Cells from Peripheral Blood Hematopoietic Progenitor Cells. J Vis Exp. (95):52298.

Wang T, Choi E, Monaco MCG, Campanac E, Medynets MA, Do T, Nath, A. (2013). Derivation of Neural Stem Cells from Human Adult Peripheral CD34+ Cells for an Autologous Model of Neuroinflammation. PLoS ONE, 8(11): e81720.

Johnson TP, Tyagi R, Lee PR, Lee MH, Johnson KR, Kowalak J, Elkahloun A, Medynets MA, Hategan A, Kubofcik J, Sejvar J, Ratto J, Bunga S, Makumbi I, Aceng JR, Nutman TB, Dowell SF, Nath A. (2017). Nodding syndrome may be an autoimmune reaction to the parasitic worm Onchocerca volvulus. Science Translational Medicine, 9(377): eaaf6953.

Demar JC, Clarkson ED, Ratcliffe RH, Campbell AJ, Thangavelu SG, Herdman CA, Leader H, Schulz SM, Marek E, Medynets MA, Ku TC, Evans SA, Khan FA, Owens RR, Nambiar MP, Gordon RK. (2010). Pro-2-PAM therapy for central and peripheral cholinesterases. Biol Interact., 187(1-3):191-8.

Gordon RK, Campbell AJ, Nambiar MP, Owens RR, Ratcliffe RH, DeMar JC, Davis AR, Hyson LA, Khan FA, Marek E, Medynets MA, Oguntayo SA, Thangavelu SG. (2008). Pro-2-PAM: The First Therapeutic Drug For Reactivation Of Organophosphate-Inhibited Central (Brain) And Peripheral Cholinesterases. 2008 Dec 1. Conference Paper.

Barone JR, Medynets MA. (2007). Thermally processed levan polymers. Carbohydrate Polymers, 69(3): 554-61.

AWARDS

Paul A. Siple Award for the Best Research Paper, 26th Army Science Conference, Baltimore, MD, Dec. 1-4, 2008

Distinguished Achievement Award, Kelly Government Solutions, Rockville, MD, Jan. 30, 2013

MEMBERSHIPS

Women Engineers Society, 2002-2006

Phi Theta Kappa Honor Society, 2002-2006

Rock Creek Riding Club, 2009-2011

United States Parachute Association 2018 - present



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