SHIPRA GUPTA, Ph.D.
LinkedIn Profile: http://www.linkedin.com/in/shipraguptabiochemistry An enthusiastic and self-motivated scientist with 15 years of experience in molecular and cell biology, biochemistry and biotechnology with knowledge of programming languages like Python, R, and SAS. Strong problem-solving and multi-tasking skills. Goal oriented with exceptional laboratory and project management skills and ability to work independently and as a team. Trained and certified in BSL-2 and radiation, biological and chemical hygiene laboratory safety and the appropriate handling and disposal of hazardous waste. Worked closely with cross-functional experts and trained and mentored graduate and undergraduate students. Contributed to writing NIH grant proposals and grant renewals. Specialties:
- Protein expression and purification
- Mammalian cell culture (HEK, CHO cell lines etc. and Oocytes), patch clamp, membrane vesicles
- Plant tissue culture
- Molecular biology techniques including gDNA isolation, cloning, PCR, Western/Southern Blot
- HPLC, AKTA, CD & Flow Cytometry
- Fluorescence microscopy using various fluorescent ligands, Confocal, smFRET, Unnatural amino acids
- Live Cell Imaging and Single Molecule Spectroscopy
- Anaerobic techniques, Schlenk Line, COY Chamber, Microfluidics
- Computer programming language: Python, R, and SAS
- Proficiency in Canvas, GraphPad Prism, Sigma Plot, SnapGene, Origin, ImageJ and MS office
CRISPR/Cas9 Crash Course for Beginners, Udemy (Feb 2020) ude.my/UC-93cabf23-ddcf-440c-aecc-9a55462d5814
Programming for Everybody (Python), University of Michigan via Coursera (Dec 2014) https://www.coursera.org/account/accomplishments/certificate/W2VVNKFQEL SAS Programming 1: Essentials, SAS.com (September 2014) Computational Molecular Evolution, Technical University of Denmark via Coursera (Feb 2014) An Introduction to Interactive Programming in Python, Rice University via Coursera (Dec 2013) https://www.coursera.org/maestro/api/certificate/get_certificate?verify-code=GNFRBNGFSH Experience:
University of Texas at Austin, Department of Neuroscience, TX, USA Position: Research Engineering/Scientist Associate IV (March 2018-Present); Specialization: Molecular Biology
Uncover the mechanism of ion channel behavior by examining how ligand binding drives molecular function of ion channels at the single molecule level with fluorescence spectroscopy using cell culture-based assays involving non-canonical (unnatural amino acids). Michigan State University, Department of Biochemistry & Molecular Biology, MI, USA Position: Research Associate (Feb 2011-May 2012); Specialization: Biochemistry & Molecular Biology Responsible for elucidating lipid metabolism by determining interaction of caveolin-1 (an integral membrane protein) with various other lipids/proteins. Actively contributed in projects involving Plin-2
(ADRP), and L-FABP.
Michigan State University, Department of Biochemistry & Molecular Biology, MI, USA Position: Research Associate (April 2007-Jan 2011); Specialization: Biochemistry & Molecular Biology Investigated heme A biosynthesis by characterization of heme A synthase and its reaction mechanism. Actively participated in writing a NIH grant proposal and trained and supervised team members with diverse backgrounds.
International Centre for Genetic Engineering and Biotechnology (ICGEB), New Delhi, India Position: Research Associate (March 2006-Feb 2007); Specialization: Biotechnology Responsible for expression, purification of calcineurin B Like protein (CBL) and standardization of its kinase reaction along with identification of various interacting partners of CBL and CIPK in plants using yeast as a model system. Isolated promoters of CBL and CIPK from pea using DNA library screening.
University of Delhi, Department of Environmental Biology/Studies, Delhi, India Position: Research Scholar (Aug 2001-Feb 2006); Specialization: Biotechnology Raised antibiotic marker free codA transgenics of Brassica juncea cv. Varuna (Indian mustard) with improved abiotic stress tolerance.
University of Delhi, Department of Environmental Biology/Studies, Delhi, India Position: Masters’ Scholar (Jun 1999-July 2001); Specialization: Environmental Biology/Studies Determined the potential of various waste biomass materials like activated sludge, onion peels etc. to adsorb/remove Cr(VI) from an industrial effluent(s) and synthetically made Cr(VI) solution. Publications:
Huang H, McIntosh AL, Landrock KK, Landrock D, Storey SM, Martin GG, Gupta S, Atshaves BP, Kier AB and Schroeder F (2015) Human FABP1 T94A Variant Enhances Cholesterol Uptake. Biochim Biophys Acta 1851: 946-955.
Najt CP, Lwande JS, McIntosh AL, Senthivinayagam S, Gupta S, Kuhn LA and Atshaves BP (2014) Structural and Functional Assessment of Perilipin 2 Lipid Binding Domain(s). Biochemistry 53: 7051- 7066.
McIntosh AL, Huang H, Storey SM, Landrock KK, Landrock D, Petrescu AD, Gupta S, Atshaves BP, Kier AB and Schroeder F (2014) Human FABP1 T94A Variant Impacts Fatty Acid Metabolism and PPAR-α Activation in Cultured Human Female Hepatocyte. Am J Physiol Gastrointest Liver Physiol 307: G164-G176.
Huang H, McIntosh AL, Martin GG, Landrock KK, Landrock D, Gupta S, Atshaves BP, Kier AB, and Schroeder F (2014) Structural and Functional Interaction of Fatty Acids with Human Liver Fatty-Acid Binding Protein (L-FABP) T94A Variant. The FEBS J 281: 2266-2283. Martin GG, McIntosh AL, Huang H, Gupta S, Atshaves BP, Landrock K, Landrock D, Kier AB and Schroeder F (2013) The human Liver Fatty Acid Binding Protein T94A Variant Alters Structure, Stability, and Interaction with Fibrates. Biochemistry 52: 9347-9357. McIntosh AL, Senthivinayagam S, Moon KC, Gupta S, Lwande JS, Murphy CC, Storey SM and Atshaves BP (2012) Direct Interaction of Plin2 with Lipids on the Surface of Lipid Droplets: A Live Cell FRET Analysis. Am J Physiol Cell Physiol 303: C728-C742. Sharmila P, Phanindra MLV, Anwar F, Singh K, Gupta S and Pardha Saradhi P (2009) Targeting Prokaryotic Choline Oxidase into Chloroplasts Enhance the Potential of Photosynthetic Machinery of Plants to Withstand Oxidative Damage. Plant Physiol Biochem 47: 391-396. Gupta S, Srivastava S and Pardha Saradhi P (2009) Chromium Increases Photosystem 2 Activity in Brassica juncea. Biol Plant 51: 100-104.
Gupta S, Sharmila P, Burma PK and Pardha Saradhi P (2006) Raising Antibiotic Marker Free Abiotic Stress Tolerant Brassica juncea Transgenics. In: Environmental Science and Technology. Eds: SK Starrett, JJ Hong and WG Lyon, American Science Press, Houston, ISBN: 0-9768853-7-9, Vol II: 501- 507. Awarded with “Best Young Scientist Paper Award" for the same. Sharmila P, Gupta S, Anwar F and Pardha Saradhi P (2006) Introduction of Glycinebetaine Pathway Through Genetic Engineering Enhances Potential of Plants to Withstand Abiotic Stress. In: Current Concepts in Botany. Eds: KG Mukerji and C Manoharachary, I. K. International Pvt. Ltd., India, 471-491. Gupta S, Srivastava S and Pardha Saradhi P (2005) Chromium (VI) Promotes Photosystem II Activity in Brassica juncea. In: Photosynthesis: Fundamental aspects to global perspectives. Eds: A van der Est and D Bruce, Alliance Communications Group, USA 2: 649-651. Education:
2006 Ph.D. in Environmental Biology/Studies
University of Delhi, Delhi, India
Thesis-Raising Antibiotic Marker Free codA Transgenics of Brassica juncea cv. Varuna with Improved Abiotic Stress Tolerance.
2001 Master of Science in Environmental Biology/Studies University of Delhi, Delhi, India
Dissertation-Potential Utilization of Waste Biomass Materials for Sorption of Chromium(VI) at Department of Energy Studies, IIT, Delhi, India.
Select Conferences/ Symposium/Workshops:
● The 29th Summer Symposium in Molecular biology presents, “Frontiers in Metallobiochemistry” at Penn State’s University Park campus, PA, USA, June 2-5, 2010.
● Gordon Research Conference on “Tetrapyrroles, Chemistry and Biology of” at Salve Regina University, Newport, RI, USA, July 20-25, 2008. (Sponsored by Chair’s Fund)
● 2nd International Conference on “Environmental Science and Technology” at Wyndham Greenspoint Hotel, Houston, Texas, USA, August 19-22, 2006. (Best Young Scientist Paper Award)
● National Conference organized by Delhi University Botanical Society on “Biodiversity Related International Conventions: Role of Indian Scientific Community”, March 8-10, 2006, New Delhi, India.
(Best Poster Award)
● 13th International Photosynthesis Congress, August 29 September 03, 2004, Montreal, Canada. Awards, Fellowships and Activities:
● Associate Editor for the “3Biotech” journal and Reviewer for the “3Biotech”, “Biomedical and Pharmacology Journal”, and “Molecular Breeding” Journals.
● Qualified CSIR-JRF examination, ranked among TOP 20% in December 2001 conducted by Council of Scientific and Industrial Research, India and received JRF and SRF for Ph.D.
● Served as President of Indian Student Organization and organized various events at MSU (2009- 2010).
● Enjoy travelling, photography, writing, cooking, music and movies of all kinds. References: On Request