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NGS, molecular biology, chemistry and biochemistry

Location:
Portland, OR
Posted:
May 15, 2020

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Resume:

Chunliu Pan U.S. CITIZEN

***** ** **** **, ********, OR 97229

Phone: 217-***-****

Email: adc8tw@r.postjobfree.com

Education

Florida Atlantic University Boca Raton, FL, USA

Ph.D. in Chemistry and biochemistry April 2012

Jilin University Jilin, P.R. China

M.S. in Material science August 2003

Jilin University Jilin, P.R. China

B.S. in Chemistry

August 2000

Work Experience

Centrillion Genomics Technologies, Hillsboro, Oregon Portland, OR Research staff 2018 - present

• Clinical genetic testing of vision-related, inherited conditions covering retinal dystrophy, developmental eye diseases

• Genomic DNA extraction, target enrichment (PCR and Long range PCR) and NGS library preparation

• Copy number variants (qPCR), automated liquid handling and DNA immobilization and purification (SPRI)

• DNA fragmentation, ligation, hybridization, Miseq and Hiseq sequencing and machine maintenance Roswell Park Cancer Institute, Department of Cancer Genetics Buffalo, NY Research Associate 2013 - 2017

• Identification of cytokines regulating androgen deprivation therapy (ADT)-induced sarcopenia in prostate cancer

• Prevention of ADT-induced sarcopenia by myokine inhibition in preclinical studies

• Expression and production of proteins by using various host cell system.

• growth of bacteria and preparation of plasmid DNA Loyola University Chicago, Department of Surgery and the Oncology Chicago, IL Research Associate 2012 - 2013

• Scrutinization of ATRA-induced differentiation on Pten-/- MLL-AF9 murine leukemia cells and differentiation of human leukemia cell lines induced by ATRA, Pten inhibitor or combination of ATRA and JNK inhibitor

• Exploration of TNFα-stimulated leukemogenesis in Pten-/- mouse bone marrow-derived haematopoietic stem and progenitor cells (HSPCs)

Florida Atlantic University, Department of Chemistry and Biochemistry Boca Raton, FL Research Assistant 2006 - 2012

• Investigation of neuroprotective characteristics of inhibitory neurotransmitter and nerve growth factor on disease-stressed primary cortical neurons or cell lines such as rat pheochromocytoma PC12 cells and human neuroblastoma cells etc

• Examination of signaling pathways induction and inhibition effects of the endoplasmic reticulum (ER) stress under stroke related conditions

• Synthesis of mesoporous materials with characteristics of high surface area and thermal stability Skills

• Molecular Biology techniques – NGS library preparation, Hybridization, Miseq and Hiseq sequencing, qPCR, SPRI, qRT- PCR, Agarose gel electrophoresis, FACS, ELISA immunoassays, Immunohistochemistry, Plasmid or protein expression and purification, SDS-PAGE and western blotting analysis, Ultrasound imaging, DNA and RNA extraction, Genotyping, Cell culture, Animal handling, Mouse breeding, Transplantation and xenograft, etc

• Chemical analytical techniques - X-ray diffraction (XRD), Infrared spectroscopy (IR), Transmission electron micrograph

(TEM), Scanning electron micrograph (SEM), Nuclear magnetic resonance spectroscopy (NMR), Mass spectrometry (MS), Gas chromatography

• Computational skills - Matlab, Origin and solid works for data and/or programming

• Software – Copycaller, Amira, Jmp, DeltaGraph, Affinity designer etc Selected Publications

Chunliu Pan, Neha Jaiswal Agrawal, Yanni Zulia, Shalini Singh etc. Prostate tumor-derived GDF11 accelerates androgen deprivation therapy-induced sarcopenia, Journal of Clinical Investigation Insight, 2020, 5(6):1

Chunliu Pan, Shalini Singh, Deepak M. Sahasrabudhe, Joe V. Chakkalakal, John J. Krolewski, Kent L. Nastiuk. TGFβ Superfamily Members Mediate Androgen Deprivation Therapy-Induced Obese Frailty in Male Mice,Endocrinology.2016,157: 4461

Chunliu Pan, Howard Prentice and Jang-Yen Wu. Beneficial effect of taurine on hypoxia- and glutamate-induced endoplasmic reticulum stress pathways in primary neuronal culture. Amino Acids, 2012, 43(2): 845

Chunliu Pan, Shi Yuan and Wenxiang Zhang, A neutral template route to mesoporous titanium phosphate molecular sieves with enhanced thermal stability, Applied Catalysis A, 2006, 312: 18



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