Sizu Mittal

** ********** *****, ********, **, L*Y4Z8

226-***-****, adc78o@r.postjobfree.com

Professional Summary

A multi-skilled, and confident person with strong scientific background and experience in different aspects of a Biotechnology lab in Molecular Biology techniques including literature review, process and procedure development. Expert in DNA/protein techniques. Highly adaptable and have an excellent on the floor safety track record. Excellent communicator who can communicate well with different people and has flexibility of working as a team member or an individual. At present, seeking an opportunity to utilize my skills and knowledge in a research lab or in a Biotechnology industry.

Areas of expertise

-DNA/ Protein tech.

-PCR/ Cloning

-Cell cultures and protein expression

-Data analysis and technical writing and documentation

-Instrumentation and analysis

- Chromatography and Spectroscopy

- Immunological techniques

Personal Skills

-Attention to detail

-Focused and Quick learner

-Team player

-Self- Motivated

-Organization

Key Skills and Competencies

Safety

Adhere to company safety policy and GMP practices.

Creating a safe working environment.

Risk and hazard assessment.

Basic laboratory safety and safe handling of chemicals.

Knowledge in using WHMIS and MSDS.

Knowledge in safe disposal of chemical and biohazardous waste materials.

Safety in machine operation and safe handling.

Use of PPEs.

Participating in safety talks and meetings.

Enforcing strict safety measures in workforce.

Laboratory

Strict adherence to GLPs all the time.

Knowledge of SOPs, GMPs, and WHMIS.

Knowledge and practical experience of various chemistry techniques:

-Titrations, dilutions and preparation of standards

-Analytical and biochemical analysis of chemical compounds or metabolites.

-Volumetric, colorimetric and gravimetric analysis and calculations.

Glassware’s used in chemical, biochemical and organic chemistry laboratory.

Knowledge of analytical instruments:

-IR Spectroscopy, FTIR, UV/Visible Spectroscopy, Atomic Absorption, HPLC, and GC

Standard curve technique (Calibration Curves) in the analysis of samples.

Analytical calculations including conversion and dilution calculations.

Communication

Strong interpersonal and communication skills.

Well experience in interpreting analytical instrument outputs (chromatograms, absorbance to concentration data and so on.) and maintaining lab reports and records.

Shows a high degree of interest in learning new things.

Microbiology

Media preparation and sterilization, autoclaving, preparation of agar plates, inoculation, culturing of microorganisms, Serial dilutions, and genetic toxicology assays (Ames Test).

Molecular Biology

Practical experience with and understanding of:

DNA purification (from different cell types agarose gels SDS-PAGE, Gel Electrophoresis), UV quantification of DNA, Centrifugation, Restriction digestion, calculation of DNA fragment sizes, cloning, preparation of transformation- competent cells, heat shock transformation, use of selective media, PCR, Southern Blotting, Hybridization, Gel extraction, purification and ligation.

Cell Biology

Knowledge and complete understanding of Culture of adherent and non-adherent cell types.

Interested in working with cancer projects, looking for new methods for diagnosis and treatment.

Batch and microbial fermentation of sucrose to ethanol and ethanol to acetic acid.

Protein Biochemistry and Immunology

ELISA, southern Blotting, Western Blotting, Affinity chromatography and Bradford assay.

Additional Knowledge

Use of SOPs, analytical methods, documentation including use of a formal lab notebook and written reports.

Practical experience in microbiology, Molecular Biology, Biochemistry, Cell Biology and Chemistry.

Knowledge of flow cytometry and its applications.

Education

Master of Medical Biotechnology University of Windsor, Canada. (2018- Dec 2019)

Advanced graduate course with a business-oriented perspective. This program allows to broadening students’ knowledge from lab work to the business sector.

Knowledge of Blackboard Learning system.

Relevant Subjects: Human Physiology and Mechanism of Disease, Biochemistry and Cell Biolipids and Membrane, Protein structure and function, Drugs from discovery to market, Clinical Biochemistry, Leadership, and Strategic Management.

Master’s in biotechnology, Kurukshetra University, India. (2010-2012)

The Graduate program in Biotechnology providing advanced knowledge and understanding of Biotechnology which combines Biology, biochemistry and biomedicine and its related fields. Understanding biotechniques like flow cytometry and microscopy.

Relevant Subjects: Microbiology, Molecular Biology, Immunotechnology, Cell and Tissue Culture, Microbial Biotechnology, Plant Biotechnology, Molecular Genetics, Animal Biotechnology, Environmental Biotechnology.

Bachelor’s in Biotechnology, Kurukshetra University, India. (2007-2010)

Relevant Subjects: Biotechnology, Chemistry (Organic, Inorganic and Physical), Zoology.

Employment Experience

Problem Analyst, at Lifelabs Toronto, Ontario Feb 2020-

Joined as Problem analyst for Cancer care Ontario’s Fecal Immunochemical Testing Program (CCO-FIT), impacting lives of hundreds of Canadians through this program with consistently

high productivity (35-40/hr). problem-solve by complete missing information under minimum supervision while maintaining high standards of patient confidentiality.

Lab Assistant at Allele Life Science, India 2015-2018

Perform quality testing of samples before carrying out research and submitting report to the client.

Maintained records and data entry about research progress.

Performed Biotech projects like cDNA preparation, PCR, Plasmid isolation, DNA isolation, Bacterial cell culture preparation, culture plates and troubleshooting the protocol.

Assisted seniors and supervised and managed student groups (5-10) in completing research projects.

Conducted a literature review for research projects and completed a research project.

Assisted in setting up and performing method development and validation of certain assays.

Attended research meetings and seminars along with senior supervisors.

Worked patiently in a fast-paced highly professional environment.

College Lecturer July-Dec 2015

Tutored Master’s and bachelor’s students.

Prepared assignments and assessment work.

Designed curriculum and exam papers.

During teaching I learned time management, patience, imagination, risk-taking, communication, teamwork, adaptability, and leadership skills.

Publication

Published a research paper in the International Journal of Science and Research (IJSR) on the isolation of mRNA from neonatal jaundice patients and studied the expression of Glucose- 6- phosphate Dehydrogenase gene through cDNA synthesis and amplification. https://www.ijsr.net/archive/v4i8/SUB157567.pdf

Projects:

1.Did Market Research for a client as a project not included in course. Provided with suggestions for the problems faced by them and skilled team-management, leadership and communication skills.

2.Did protein isolation, purification and expression in bacterial cells using pFU plasmid vector. Performed Bradford’s test for determining the concentration of protein and SDS-PAGE for determining protein in the sample.

3.Presented on Necrotizing Fascitis during graduation. Did a literature review about the disease occurrence, symptoms and discovered treatment methods for the disease?

4.Presented on HIV protease inhibitors during graduation. Did a literature review about the disease and drugs developed for the treatment of disease. Stages in the development of drugs from finding the lead compound to its modifications and Pharmacokinetic properties of the drug.

5.Prepared project on medical devices in which we compared two different medical devices and prepared a report and presentation for Clinical Biochemistry. This was to study Point of care testing, in-home use tests and, lab use tests.

Extra-curricular activities:

Joined as a volunteer for UWindsor Stem cell Club 2019, organized by Canadian Blood Services. Collected DNA samples from individual’s saliva through swabbing.

Volunteer at Open Street festival, Windsor, 2019.

Volunteer at Windsor Youth Centre, Windsor, Ontario (Jan 2019- Dec 2019).

Volunteer for Lancer Leadership Conference, 2019.

Judge for Windsor Regional Science, Technology and Engineering Fair (WRSTEF), 2019.

Participated in Bayer Digital Campus Challenge, 2018.

Attended Biotechnology and life science workshops.

2-month Internship at Orbit Biotech Pvt. Ltd, Mohali, Punjab, India.

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