Dmitri Sitnikov

* ***** **., ******, **, *****, USA 514-***-****, adc3jj@r.postjobfree.com

Expert in metabolomic and proteomic LC-MS analysis

Expert in quick development, validation and implementation of quantitative LC-MS analysis, ICH and FDA regulations

Inventor and expert in design of platforms based on LLE, SPE, affinity on-line and off-line chromatography/robotics methods for metabolomics and proteomics.

> 15 years in biomarker discovery, quantitation of metabolites and proteins; ADME.

10 year of GLP environment, multidisciplinary project coordination and process management

Personnel coaching and training

Expert analytical techniques: SEC, IEX, RP, HILIC, mixed modes, chiral, UV-Vis DAD, light scattering, ECD, MS, MS/MS), ELISA, SDS-PAGE, Western blot, protein quantitation, DSC, atomic flame spectrometry, infrared spectrometry, robotic equipment)

Work experience:

2012-current, Research assistant, Laboratory of Dr. Vuckovic, Chemistry and Biochemistry, Concordia University, Montreal

New method development, validation and execution of multiple sample preparation, quantitation and identification experiments by LC-MS:

oamino acids and sugars in yeast culture media (QTOF)

osteroids in blood and saliva (MRM)

o27 neuromediators and hormones in blood (ACTH, thyroxine, Dopamine, TRH, etc.) (MRM)

Discovery and characterization of diosmin and conjugates in mice tissues and blood in the context of ADME study.

Work on and troubleshoot: HP1290 (Agilent), Acquity (Waters), QQQ (6450), QTOF (Agilent 6545, 6550), ion-mobility/QTOF (Agilent6560), LTQ-Orbitrap

Identification of metabolites via DDA and DIA MS2 (QTOF, LTQ Orbitrap) and spectral libraries (Agilent)

Analysis of targeted and global data (Mass Hunter, Lipid Search, Xcalibur, Proteome discoverer, MZvault)

Comprehensive training for novel graduates on LC-MS and data analysis (5 students)

2006 –2010 Sr. Scientist, Sample Processing Leader, Caprion Proteomics, Montreal

Process management (5 years experience, approximately 10 proteomic production projects)

GLP. Wrote and implemented 20 SOP’s, QC and QA protocols.

R&D. Development and implementation of plasma preparation for proteomic analysis. 1.Depletion methods using MARS and IgY14 based depletion columns. Modification of controlled proteolysis protocols HPLC SCX based plasma sub-fractionation for off-line proteomics. Data analysis (peptide shifter).

Developed and troubleshoot multiple novel sample processing (tissue proteolysis in ultrafiltration devices) and data analysis methods which allowed to speed and simplify the preparation process 2-3 fold. Promoted new ideas (proteome decomplexation via glyco-/phospho- affinity subtractive fractionation).

Laboratory management. Organized and maintained the compliance of sample processing to safety and consistency policies. Establishment and operation of biosafety class 2 sample preparation and LC-MS analysis laboratory. (tuberculosis, brucellosis, hepatitis).

Represented company internally and externally (Pittcon, HUPO). Trained colleagues and managed lab technicians (1). Drafted articles, presentations and scientific proposals.

Managed the entire proteomic profiling project for three different cancers. By combining functional protein information (protein pathways) and expression data, the group identified at least 3 putative key regulatory proteins common for the selected cancers.

Managed and coordinated long term projects aimed on the collection of biological samples from around the world. Communicated with clients, management and partners on the scheduled and occasional meeting. Reported to Directors and external clients.

2001 - 2006 Scientist I and Scientist II at Protein Analysis, Caprion Pharmaceuticals, Montreal

Invented and implemented the use of volatile buffer in IA depletion of blood plasma, resulted in 2-fold increase

in recovery. Publication released on the topic

Preparation of samples for proteomic analysis from biological fluids and tissues. Synthesis, modification and

purification of peptides. N-terminal sequencing and quantitation. (HPLC-SCX, SAX, RP, affinity, normal

phase, SEC). Analysis of peptides on LC-MS and MALDI (QTOF Altima API, Waters)

2000 - 2001 Research Assistant, Structural Protein Chemistry, BRI, Montreal, Canada

Synthesis and purification of recombinant proteins for NMR; Assessment of physical and chemical status of proteins (HPLC, DSC, Western blot, enzyme kinetic, UV/Vis spectrometry, ESI-MS). Protein refolding.

Education:

2020 PhD Student, Laboratory of Dr. Dajana Vuckovic, Chemistry Department, Concordia University, Montreal.

Characterization of global and targeted LC-MS metabolomics analysis of human plasma and saliva with focus on CNS-related regulatory hormones.

Invented, implemented and validated the sequential sample preparation platform for targeted/global metabolomics, quantitation and identification of metabolites in multiplex LCMS analysis

1998 Master of Science in Microbiology, McGill University, Montreal, Canada

1989 Bachelor of Science in Epidemiology, Medical Academy of Hygiene and Sanitation,

St-Petersburg, Russia

Publications:

Dmitri Sitnikov, Dajana Vuckovic, “Sequential sample preparation for global metabolomics analysis of human blood plasma.” In preparation

Dmitri Sitnikov, Dajana Vuckovic, “Quantitative assessment of cortisol and cortisone in human saliva by tandem LC-MS/MS. Method Validation.” In preparation

Dmitri G. Sitnikov, Cian S. Monnin and Dajana Vuckovic “Systematic Assessment of Seven Solvent and Solid-Phase Extraction Methods for Metabolomics Analysis of Human Plasma by LC-MS” Sci. Rep. 6, 38885 (2016).

Lamontagne J, Forest A, Marazzo E, Denis F, Butler H, Michaud JF, Boucher L, Pedro I, Villeneuve A, Sitnikov D, Trudel K, Nassif N, Boudjelti D, Tomaki F, Chaves-Olarte E, Guzmán-Verri C, Brunet S, Côté-Martin A, Hunter J, Moreno E, Paramithiotis E “Intracelullar adaptation of Brucella abortus” J Proteome Res. 2009 Mar;8(3):1594-609.

Sitnikov D, Hunter JM, Hayward C, Eng K, Migneault I, Tessier S, Opiteck GJ, Kearney P. “Peptide shifter: enhancing separation reproducibility using correlated expression profiles” J Am Soc Mass Spectrom. 2007 Sep; 18 (9):1638-45.

Dmitri Sitnikov, Donovan Chan, Eric Thibaudeau, Marc Pinard, Joanna M. Hunter. “Protein depletion from blood plasma using a volatile buffer” Journal of Chromatography B, 832, 2006, 41-46

Pollock S, Kozlov G, Pelletier MF, Trempe JF, Jansen G, Sitnikov D, Bergeron JJ, Gehring K, Ekiel I, Thomas DY. Specific interaction of ERp57 and calnexin determined by NMR spectroscopy and an ER two-hybrid system” EMBO J. 2004, 23, 1020-1029.

Doctoral Student award in Preventive Health Research

Perform Center of Concordia University

May 2014-May 2016

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