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PCR, Recombinant protein purification, cell culture

Location:
Princeton, NJ, 08540
Posted:
February 22, 2020

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Resume:

Meena Tomar

** ******** **., *********, ** *****

Phone: 201-***-****, Email: adbxcd@r.postjobfree.com Career Objective:

With my research experience in molecular biology, recombinant protein purification and drug discovery, I am looking for a challenging and growth oriented position in a company dedicated to Science and Development. I bring an expertise in research development “can do” attitude”, scientific knowledge and ability to collaborate with internal and external colleagues.

Skills:

Protein Expressions and purification of recombinant proteins with different affinity tags, preparative column chromatography, membrane filtration, dialysis, SDS-PAGE, Western blot, Spectroscopy, Protein Assays, ELISA

Molecular Biology Preparation and column purification of plasmid DNA, Bacterial Transformations

(Heat shock and electroporation), Ligation, Restriction Digest, Genomic DNA extraction, Competent cells, Site directed mutagenesis, PCR (Real Time qPCR, RAPD, Multiplex), Agarose Gel Electrophoresis, DNA Sequencing Drug Discovery Cell based assays (Luciferase reporter assay, TR-FRET Assay, Cytotoxicity Assay)

Bacteriology Inoculation, Culture transfers, Prepare media, Pour plates, Single colony isolation, Endospore stain, glycerol stocks

Cell Biology Good Laboratory Practice (GLP) and Safe Laboratory Practice (SLP), reporter assay, cell-counting (Haemacytometer), Aseptic technique, cell passage, cell staining, cryopreservation

Data Analysis Biotechnology Software: ClustalOmega, NCBI BLAST, GraphPad, ExPASy General Software: Microsoft Excel, Word and Powerpoint Education:

M.S. in Biotechnology – The Johns Hopkins University, Baltimore, MD GPA 3.7/4.0 Graduate Certificate in Molecular Biology – Thomas Jefferson University, Philadelphia, PA GPA 3.9/4.0 Work Experience:

Associate Research Scientist Femeris – MDL LLC, Hamilton NJ May 2016 – Current Working with senior scientists and other team members to design and execute experiments to develop molecular based technology for the detection and treatment of obstetric and gynecologic infectious diseases, antibiotic resistant bacterial infections, and assay design for drug discovery process. Specifically -

● Detect multiple pathogens as well as certain drug resistance profiles from a single swab by various sub types of PCR testing.

● Expression and Purification of recombinant proteins with different affinity tags

● Provide scaled up purification of lead molecules for stability studies

● Design, validate and execute drug target assays, cell based assays and MIC assays

● Design and set up experiments for antimicrobial resistance emphasizing on cell wall biosynthesis inhibitors

● qPCR assay design and validation based on CLSI guidelines

● Perform gene and recombinant DNA cloning, including preparation of plasmid/chromosomal DNA, analysis of recombinant plasmids, quantitation of DNA, confirmation of successful cloning of functional domains using ClustalOmega, ExPASy, NCBI BLAST;restriction endonuclease digestion; archiving the strains

● Preparation of liquid and plate media, running and evaluation of SDS-PAGE as well as agarose gels, genomic PCR, Real Time PCR, cloning full length genes by PCR, site directed mutagenesis, sub cloning to expression vectors

● Maintain lab book with records and data, collection and preparation of data for analysis, analyze and interpret using Excel and GraphPad; present results data to the director and senior scientists

● Manage laboratory functions such as organization, ordering of supplies and scheduling equipment use Recognitions and Awards:

● Recognized 3 times consecutively on Annual GBG(Genesis Biotechnology Group) Research Poster day

● Overall Outstanding Academic Performance Award in Biotechnology program, Thomas Jefferson University

● Biotechnology Achievement Award, Montgomery College, Germantown, MD Work Status: United States Citizen

References:Available on request



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