CURTIS OMAR GREEN

* ******* **, ****, **, Canada M6N 4W8

Cell Phone: 437-***-****

adblz5@r.postjobfree.com or adblz5@r.postjobfree.com

LABORATORY AND TECHNICAL SKILLS

Experienced in applying molecular biology and cell culture techniques such as culturing mammalian cells, RNA isolation, cDNA synthesis, gene expression studies, conventional and real-time PCR, gel electrophoresis and single cell sequencing

Experienced with performing biochemistry techniques to purify proteins, these include producing a homogenate, ion exchange chromatography, Bradford assay

Experienced with performing chemistry techniques including ion ratio and GC mass spectrometry, HPLC analysis, extraction and isolation of novel compounds

Experienced in performing nutrition related studies such as preparing nutritional supplements containing amino acids, vitamins etc and subsequent analysis of biological specimen including blood, urine and saliva.

Experienced in developing and managing chemistry and molecular/cell culture laboratories including development of SOPs and quality management systems, project protocols and scientific documents.

Familiar with ordering, monitoring and maintaining laboratory inventories of biological/chemical agents, supplies as well as maintaining records and laboratory data.

Familiar with statistical software for analysing data including STATA, SPSS and PRISM

Experienced in using Microsoft Office (Word, Excel, PowerPoint)

EDUCATION/QUALIFICATION & GRANTING INSTITUTIONS

2009 Ph.D. Biochemistry, University of the West Indies Mona (UWI), JA

Thesis Title: “Characterization of polymethoxylated flavones in Ortanique peel and the in vivo metabolic effects in animal model of hypercholesterolemia”

Supervisor: Professor Helen Asemota

2004 BSc. (Honours) Biochemistry and Chemistry, University of the West Indies Mona (UWI), JA

POST-DOCTORAL TRAINING AND EXPERIENCE

2019: Global Challenges Research Fund (GCRF) Research fellowship for training at the Institute of Developmental Sciences (IDS), University of Southampton, UK

2017: Training in Advanced Molecular Biology and Cell Culture technologies at the Institute of Developmental Sciences (IDS), University of Southampton, UK

Training included the following technologies:

Molecular Biology/Cell Culture technologies involving the use of myoblasts and muscle samples:

Reviving myoblasts, Immunofluorescent staining: Desmin and DAPI stains, Cell proliferation and survival assays, Seahorse assay (cell metabolism), Differentiation of myoblasts, Drop Seq (for single cell RNA sequencing), Transfection of myoblasts, Gene expression/epigenetic Studies, Immunohistochemistry, Proteomic, Protein extraction from whole blood

2014: Training in Primary Cell Culture techniques- Singapore Collaborators,

Techniques included:

Processing human muscle biopsy to isolate myoblasts

Culturing myoblasts isolated from muscle biopsies

Passaging human muscle myoblasts and monitor growth until confluent enough for harvesting

2012: Post-doctoral training in Molecular Biology and Tissue Culture techniques with applications in epigenetic at the Liggins Institute, University of Auckland,

Training included the following technologies:

DNA and RNA extraction from various samples including umbilical cord and buccal swabs using traditional methods as well as various kits.

a.Analysis of nucleic acid extracts using nanodrop and gel electrophoresis technologies for quality control.

b.qPCR technology

c.Cell culture: HepG cells: from reviving frozen stock culture, seeding for experiment and executing several experiments involving various models of diseases

2010: IAEA sponsored post-doctoral fellowship training at the University of Wisconsin, Madison.

Training entailed the use of stable isotope for nutritional study with specific

application in the use of labelled water for the measurement of body composition and energy expenditure using isotope ratio mass spectrometry

2009: Post-doctoral training at Tropical Metabolism Research Unit, (Caribbean Institute for Health Research) in nutritional metabolism, UWI, Jamaica 2009-2013

Received hands-on training in the following:

Use of several laboratory equipment inclusive of the GC Mass Spectrometry (GC-MS) and the High-Performance Liquid Chromatography (HPLC) for analysis of various biological samples.

Involvement in clinical research and the use of stable isotopes to assess protein and amino acid metabolism in childhood malnutrition and adult survivors of malnutrition and stroke.

Laboratory supervision/management including procurement of laboratory supplies and equipment, inventory of laboratory supplies, servicing of laboratory equipment, supervision of laboratory staff.

2007: Training at National Autonomous University of Mexico (UNAM) under the Jamaica/Mexico Programme 2005-2007, Project on the Screening, assessment and identification of anti-nutritional factors in selected Caribbean food crops and spices and the study of effects of their consumption in normal and diseased states – Phase III.

WORK EXPERIENCE

Academic year

(2013-2019) Lecturer and Laboratory Manager: Major achievements include establishing and Managing the Cell Culture and Molecular laboratories as well as training and supervision of laboratory staff. We were the first group to culture human muscle stem cells (myoblasts) in Jamaica. This has provided the platform for studying the developmental origin for various disorders including sarcopenia, diabetes and hypertension. We have been able to successful execute the research objects of major grants, produce documents for publication and new grant application.

(2009-2013) – Research Fellow: Tropical Metabolism Research Unit – Caribbean Institute for Health Research, University of the West Indies (UWI). Major achievements include the successful execution and completion of research projects involving malnourish infants. This resulted in the publication of several manuscripts in high impact journals.

(2009) Teaching Assistant: Basic Medical Sciences, UWI – Lecturer for the courses: BC10M – Pure and Applied Sciences Students and DD120 (Cell Biology) – Medical Students

(2005-2009) – Postgraduate Student Assistant: Office of Graduate Studies and Research and Examinations Section, UWI, Mona

(2004-2009) - Laboratory Assistant for courses: BC10M and BC21D; University of the West Indies, Mona

SCHOLARLY ACTIVITIES & PERFORMANCE

Journal manuscripts:

1.Migliavacca, E; Tay, S; Patel, H., ….Green, C.O, Karaz, S., Narasimhan, K., Marquis, J., Metairon, S., Nelson, G., Ngo, S., Pleasants, T., Raymond, F., Sayer, A., Sim, CM., Slater-Jefferies, J., Syddall, H., Tan, P., Titcombe, P., Westbury, L., Wong, G., Yonghui, W., Cooper, C., Godfrey, K., Sheppard, A., Lillycrop, K., Karnani, K., Moco, S., Giner, M., Vaz, C and Feige, J (2019). Mitochondrial oxidative capacity and NAD+ biosynthesis are reduced in human sarcopenia across ethnicities. Nat Commun 10, 5808 doi:10.1038/s41467-019-13694-1

2.Green, C.O., Badaloo, A., Hsu, J.W., Reid, M, Taylor-Bryan, C., Forrester, T and Jahoor, F. (2014). Effects of randomized supplementation of methionine or alanine on cysteine and glutathione production during the early phase of treatment of children with edematous malnutrition. Am J Clin Nutr 99(5): 10520-8.

3.Green, C.O., Wheatley, A.O., Bailey, D., Sotelo, A. and Asemota, H.N. (2014). Nutritional composition of Jamaican Citrus agro by-product with potential for nutraceutical product development. Research. 1: 771

4.Green, C. O., Wheatley, A.O., McGrowder, D.A., Dilworth, L.L. and Asemota, H.N (2013). Effects of Ortanique peel polymethoxylated flavones on organ function parameters in hypercholesterolemic rats. J. Food and Chemical Toxicology; 51:306-309. (Principal award for most outstanding research activity, 2014)

5.Forrester, T.E., Badaloo, A.V., Boyne, M.S., Osmond, C., Thompson, D., Green, C., Taylor-Bryan, C., Barnett, A., Soares-Wynter, S., Hanson, M.A., Beedle, A.S. and Gluckman, P.D. (2012). Prenatal Factors Contribute to The Emergence of Kwashiorkor or Marasmus in Response to Severe Undernutrition. PLoS One. 7(4): e35907. doi:10.1371/journal.pone.0035907.

6.Badaloo, A., Hsu, J.W., Taylor-Bryan, C., Green, C., Reid, M., Forrester, T., and Jahoor, F. (2012). Dietary cysteine is used more efficiently by children with severe acute malnutrition with edema compared with those without edema. Am J Clin Nutr 2012;95:84-90. (Award for Best Research Publication)

7.Green, C. O., Wheatley, A.O., Hanchard, B., Gibson, T.N., McGrowder, D.A., Dilworth, L.L. and Asemota, H.N. (2011). Histopathological alterations in organ structures of hypercholesterolemic rats fed ortanique peel polymethoxylated flavones. Basic and Applied Pathology. 4(3): 71–77

8.Green, C. O., Wheatley, A.O., McGrowder, D.A., Dilworth, L.L. and Asemota, H.N (2011). Hypolipidemic effects of Ortanique peel polymethoxylated flavones in rats with diet-induced hypercholesterolemia. Journal of Food Biochemistry; 35(5); 1555–1560.

9.Green, C. O., Wheatley, A.O., McGrowder, D.A., Dilworth, L.L. and Asemota, H.N (2012). Modulation of antioxidant enzymes activities and lipid peroxidation products in diet-induced hypercholesterolemic rats fed Ortanique peel PMFs extract. J Appl Biomed. 10(2): 91-102. ISSN 1214-0287

10.Green, C.O., Wheatley, A.O., McGrowder, D.A., Dilworth, L.L., Hanchard, B., Gibson, T.N. and Asemota, H.N. (2009). Histopathological changes in organs of diet-induced hypercholesterolemic rats fed ortanique peel polymethoxylated flavones. 18th Annual Research Conference. Theme: Child health: from the womb to adolescence. West Indies Medical Journal 58 (Suppl 4): 1-51.

11.Green, C.O., Wheatley, A.O., Osagie, A.U., Morrison, E.Y. and Asemota, H.N. (2007). Determination of polymethoxylated flavones in peels of selected Jamaican and Mexican citrus (Citrus spp.) cultivars by high performance liquid chromatography. Biomedical Chromatography, 21: 48-54.

12.Green, C.O., Wheatley, A.O., Dilworth, L.L., McGrowder, D., Mills, J., Morrison, E.Y.St.A., and Asemota, H.N. (2007). Effect of Jamaican Ortanique peel polymethoxylated flavones on kidney and liver function in diet-induced hypercholesterolemic rats. West Indies Medical Journal 56 (Suppl 3): 1-42.

Book Chapter

13.Green, C.O., Wheatley, A.O., Osagie, A.U., Dilworth, L.L., Morrison, E.Y.ST.A. and Asemota, H.N. (2009). Characterization of health-promoting polymethoxylated flavones in Jamaican and Mexican Citrus peels. In: II International Symposium on Human Health effects of Fruits and Vegetables, FAVHEALTH 2007, B. Patil (ed) International Society for Horticultural Sciences (ISHS) Acta Horticulturae 841: 511-514. http://www.actahort.org/books/841/841_71.htm

Patents granted/published

14.Green, C.O. (2015). Methods for the development of Ortanique peel polymethoxylated flavones extract powder for commercial applications. USA Patent Application, # 201******** A1. May 7, 2015). http://www.freepatentsonline.com/201********.pdf

15.Green, C.O and Asemota, H.N. (2012). Compositions and methods for treating hypercholesterolemia using Ortanique peel extract (USA Patent, # 8,268,369 B2). http://www.freepatentsonline.com/8268369.html.

Newsletter

16.Green, C.O. (2014). Therapeutic Ortanique: A treatment for high cholesterol levels. Mona; November 2014. pg 8

17.Green, C.O. (2013). Using Ortanique Peel Extract for Treating Hypercholesterolemia. TMRI FOCUS. February 2013; vol 6(1) pg 3.

PRESENTATIONS AT CONFERENCES/MEETINGS, AND TEACHING EXPERIENCE

18.Burton, M., Green, C., Francis-Emmanuel, P., Campbell, C., Godfrey, K., Forrester, T. and Lillycrop, K. Skeletal muscle gene expression in SAM survivors is associated with muscle mass and later life risk for development of Sarcopenia. (2019). Epigenomics of common diseases conference. October 2019, London, UK.

19.Green, CO., Francis-Emmanuel, P., Nelson, G., Boyne, M., Lillycrop, K., Godfrey, K., Forrester, T. (2019). Association of severe acute malnutrition in childhood with markers of sarcopenia in adult life. Royal Society Meeting. March 2019, UK.

20.Green, C.O. (2014). Methods for treating hypercholesterolemia using Ortanique Peel extract. Scientific Research Council. 3rd Biennial S&T Conference. Display Booth. The Conference Theme: Green gold: Medicinal Ganja and other natural products – a potential source of wealth. The Ballroom: Jamaica Pegasus Hotel. November 10-12, 2014.

21.Green, C.O., Wheatley, A. O., McGrowder, D.A., Dilworth, L. L., and Asemota, H. N. (2014). Ortanique peel PMFs extract (PMFort©) for hypercholesterolemia. Faculty of Medical Sciences. Competitiveness productivity and Innovation in a global environment. Principal Research days, 2014.

22.Badaloo, A., Green, C.O., Hsu, J.W., Reid, M, Taylor-Bryan, C., Forrester, T and Jahoor, F. (2013). Effects of methionine supplementation on cysteine and glutathione production in malnourished children. Caribbean Public Health Agency (CARPHA) 58th Annual CARPHA (CHRC) Scientific Meeting Hilton Barbados Resort – Barbados May 02-04, 2013.

23.Green, C.O., Wheatley, A. O., McGrowder, D.A., Dilworth, L. L., and Asemota, H. N. (2013). Hypolipidemic effects of Ortanique peel polymethoxylated flavones in rats with diet-induced hypercholesterolemia: Emerging drug development. FMS, Epidemiology Studies, Principal Research days 2013.

24.Green, C.O., Badaloo, A., Hsu, J.W., Reid, M, Taylor-Bryan, C., Forrester, T and Jahoor, F. (2012). Effects of Methionine supplementation on cysteine and glutathione production in malnourished infants. Experimental Biology 2012, April 21-25; San Diego Convention Center, CA. FASEB 26

25.Green, C.O., Wheatley, A.O., Hanchard, B., Gibson, T.N., McGrowder, D.A., Dilworth, L.L. and Asemota, H.N. (2011). Histopathological effects of ortanique peel polymethoxylated flavones on organ structures of hypercholesterolemic rats FASEB 25:616.2

26.Green, C.O., Stennett, D.K., Wheatley, A.O., McGrowder, D.A., Dilworth, L.L. and Asemota, H.N. (2010). Bioactive compounds from Jamaican Ortanique and Yams: Implications in diabetes and hypercholesterolemia. UDOP 2010 - Ninth Scientific Session sponsored by Environmental Health Foundation.

27.Green, C.O., Wheatley, A.O., McGrowder, D.A., Dilworth, L.L., Hanchard, B., Gibson, T.N. and Asemota, H.N. (2009). Histopathological changes in organs of diet-induced hypercholesterolemic rats fed ortanique peel polymethoxylated flavones. 18th Annual Research Conference. Theme: Child health: from the womb to adolescence. November 11-13, 2009. (Award for Best Poster).

GRANTS/COLLABORATION

Collaborator on various grant projects:

GCRF Fellowship 2019: University of Southampton “Molecular insights into the later life remediation of sarcopenia arising from severe acute malnutrition in early life”

Gates Foundation. Molecular pathways to stunting and associated cardiovascular/ metabolic disease. Ended December 2017.

NIH Grant. Muscle metabolic changes in high cardiovascular risk setting of stroke and impact of exercise & nutrition intervention. Ended March 2017.

NIH Grant. Energy metabolism, obesity and diabetes in African diaspora populations. Ended December 2017.

NIH Grant. METS Microbiome. Gut microbiome and obesity/diabetes in African populations 2017-2021

Nestle Nutrition Foundation Grant. Molecular pathways to muscle dysfunction and associated cardiovascular/metabolic sequelae in aging. Ended December 2018.

NIH Grant. Reinvigorating brains in adult survivors of severe childhood malnutrition. 2018-2019.

Glutathione homeostasis and oxidant damage in kwashiorkor: Role of sulphur amino acids in GSH deficiency childhood malnutrition. NIH. Contract No: 5R01 DK056689. Completed

NIH Grant. Effects of early exercise on muscle and cardiovascular health after stroke. Completed

OTHER PROFESSIONAL ACTIVITIES AND EXPERIENCE

Member of the American Society for Nutrition (ASN), 2010-present

Member of the American Physiological Society (APS), 2011-present

Member of Society for Scientific Advancement (SOSA), 2013-Present

Certificate of Participation. Cell Symposia: Transcriptional Regulation in Development and Disease. June 26-28, 2016. Chicago, USA

Certificate of Participation. Cell Symposia: Systems Approach to Metabolic Diseases. October 1-3, 2014. Chicago, USA.

National Institute of Health (NIH) web-based training course “Protecting Human Participants” Certificate of Completion, April 4, 2013

POSTGRADUATE RESEARCH AND SUPERVISION

Student Mitchele Oliver, BC36A

Focus: Development of functional foods from Ortanique

Faculty/Department: Medical sciences/Basic Medical Sciences

Year: 2009

Advisor

Student Henkel Valentine, MPHIL

Focus: Developing prostate cancer cell lines

Faculty/Department: Medical sciences/Basic Medical Sciences

Year: 2016-Present

REFERENCES

Prof. Asha Badaloo

University of the West Indies

Jamaica, WI

Email: adblz5@r.postjobfree.com

Dr. Debbie Thompson

The Hospital for Sick Children (SickKids)

Toronto, Canada

Email: adblz5@r.postjobfree.com

Dr. Patrice Francis-Emmanuel

Clinical Fellow (Specialty Registrar),

Diabetes and Endocrinology,

National Health Service, United Kingdom

Email: adblz5@r.postjobfree.com

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