Laboratory Manager Biochemist

*** ***** *** • SMITHVILLE, TX *****

PHONE 512-***-**** E-MAIL adbi2z@r.postjobfree.com

JIM PERSINGER

SUMMARY STATEMENT

I bring to your organization research, technical and management experience of 25 years in the field of biochemistry research. My passion for research and vast experience in training others in the academic setting as well as industry is at the core of who I am. I have technical and troubleshooting skills seldom found in bench scientists. My experiences are detailed below, and I look forward to bringing my expertise to your organization.

SUMMARY OF QUALIFICATIONS

Excellent troubleshooting and diagnostic skills.

Good management, communication and people skills

Extensive background in protein work.

Mastery of many forms of protein expression and purification.

Self motivated professional capable of working independently or in a team environment.

EDUCATION

Graduate course work – Southern Illinois University, 1997-1998

Bachelor of Arts –Biological Sciences- Southern Illinois University, 1992

Associate in Science and Associate in Arts – Olney Central College, 1989

PROFESSIONAL EXPERIENCE

[ 2014-Present ] MD Anderson Cancer Center Smithville, TX

Laboratory Manager

Trained and supervised other researchers, graduate students, and undergraduate student workers in the lab. Maintained supplies for the operation of the lab. Managed the accounts used in purchasing. Maintained and repaired equipment used in our research.

Interacting with vendors and maintaining contacts for all the items we need for research in the lab

Maintained laboratory compliance with regulatory groups on campus and in Houston.

Conduct ongoing research projects in the laboratory and consult and give insight into any and all projects currently underway.

Plan and organize laboratory locations and resources as personal graduate and move on and new members join the research group.

Purified protein complexes on large scale from Yeast used in laboratory research. Purified proteins for use in collaboration projects for CXMS projects and performed CXMS experiments for publications. Created and purified large scale homogeneous Carrier DNA substrates used in lab. Created and purified truncated protein constructs to examine potential protein protein interactions by specific domains of remodeling complex subunits. Studied both structure and function of an array of chromatin remodeling complexes. Have studied the domains of those complexes and how they function and interact with substrate and in autoregulation of complexes. Have created modified histone octamers to study the structure and potential elasticity of the octamer structure and its implications in chromatin arrangement in the cell along with chromatin composition.

[ 2009-2014 ] Southern Illinois University Carbondale, IL

Laboratory Manager

Trained and supervised other researchers, graduate students, and undergraduate student workers in the lab. Maintained supplies for the operation of the lab. Managed the accounts used in purchasing. Maintained and repaired nearly all equipment used in our research.

Interacting with vendors and maintaining contacts for all the items we need for research in the lab

[ 2007-2009 ] Gardner Denver Quincy, IL

Supervisor Technical Support

Provided field service and in house technical support for Gardner Denver Service department. Specialized in Reciprocating product lines for several niche market groups such as the PET industry and locomotive industry. Taught field service training courses. Provided support for field service technicians. I was promoted to the supervisor of the service training center in 2009 and oversaw the training center expansion project to house the new product groups acquired by Gardner Denver. Designed complete course work for training courses. Worked with other entities to improve products. Supervised the cylinder wall inspection project. Interviewed prospective employees. Trained field service technicians that came into the department and technicians from companies acquired by Gardner Denver. Assisted in maintaining the field service technician schedule. I maintained the service schedule calendar to insure proper maintenance items were quoted for each scheduled maintenance. During my time in the service department at Gardner Denver I developed the ability to discuss and teach complex topics to other professionals or those outside the field.

[ 1992-2007 ] Southern Illinois University Carbondale, IL

Researcher III

Trained and supervised other researchers, graduate students, and undergraduate student workers in the lab. Maintained supplies for the operation of the lab. Managed the accounts used in purchasing. Maintained and repaired nearly all equipment used in our research. Train incoming graduate students and other researchers in the use of the lab equipment. I am the first point of contact for all equipment malfunctions within the department. I coordinate with the vendor for repairs of the equipment. I have developed large-scale fermenter systems used in cell culture for both yeast and bacteria, involving agitation, temperature regulation and aeration. Placed orders and maintained accounts with the scientific vendors.

I have studied management skills which is valuable in any work environment. This has allowed me to be a trusted adviser and resource for daily operations within the lab. I have had a great attendance record in all my years of employment from early high school through college to my present job of over 12 years. My strong work ethic in the past has helped me to obtain jobs later so I know the value of doing the job right. As a researcher, I have to be able to daily diagnose what is going on in a sub microscopic environment of molecular machines and get them to do what I need them to do. I am a thinker and problem solver and know the value of the ability to find answers to the day’s questions.

PUBLICATIONS

RNA Polymerase and Transcription

Publication 1.

Probing the protein-DNA Contacts of a Yeast RNA Polymerase III Transcription Complex in a crude extract: Solid Phase Synthesis of DNA Photoaffinity Probes Containing a Novel Photoreactive Deoxycytidine Analog

Biochemistry: Volume 35, Number 30, Pages 9821-9831, 1996

Publication 2.

Mapping the contacts of Yeast TFIIIB and RNA Polymerase III at Various Distances from the Major Groove of DNA by DNA Photoaffinity Labeling

The Journal of Biological Chemistry: Volume 271, Issue of December 20, Pages 330**-*****, 1996

Publication 3.

Survey of four different photoreactive moieties for DNA photoaffinity labeling of yeast RNA polymerase III transcription complexes

Nucleic Acids Research: March 15; 26 (6) pages 1421-1426, 1998

Publication 4.

Spatial organization of the core region of yeast TFIIIB-DNA complexes

Molecular and Cellular Biology: July; 19 (7) pages 5218-5234, 1999

Publication 5.

Nuclear DEAF-1-related (NUDR) protein contains a novel DNA binding domain and represses transcription of the heterogeneous nuclear ribonucleoprotein A2/B1 promoter

The Journal of Biological Chemistry: Volume 274, Issue of Oct 22, pages 305**-*****, 1999

Publication 6.

Site-directed DNA photoaffinity labeling of RNA polymerase III transcription complexes

Methods of Molecular Biology: Volume 148, pages 363-381, 2001

Publication 7.

Purification of active TFIID from Saccharomyces Cervisiae. Extensive promoter contacts and co-activator function.

The Journal of Biological Chemistry: Volume 279, Issue of November 26, pages 499**-*****, 2004

Eukaryotic Gene Regulation

Publication 8.

An Asymmetric Model for the Nucleosome: A Binding Site for Linker Histones Inside the DNA Gyres

Science: Volume 274, Issue of October 25, pages 614-617, 1996

Publication 9.

Use of DNA Photoaffinity Labeling to Study Nucleosome Remodeling by SWI/SNF

Methods: volume 19, November, Pages 434-446, 1999

Publication 10.

The interactions of yeast SWI/SNF and RSC with the nucleosome before and after chromatin remodeling

The Journal of Biological Chemistry: Volume 276, Issue of April 20, Pages 126**-*****, 2001

Publication 11.

SWI/SNF unwraps, slides, and rewraps the nucleosome

Molecular Cell: Volume 11, February, Pages 391-403, 2003

Publication 12.

Functional role of extranucleosomal DNA and the entry site of the nucleosome in chromatin remodeling by ISW2

Molecular and Cellular Biology: Volume 24(22), November, Pages 100**-*****, 2004

Publication 13.

Chromatin remodeling by ISW2 and SWI/SNF requires DNA translocation inside the nucleosome

Nature Structural and Molecular Biology: Volume 13(4), April, Pages 339-346, 2006

Publication 14.

Architecture of the SWI/SNF-Nucleosome Complex

Molecular and Cellular Biology: Molecular and Cellular Biology, October 2008, p. 6010-6021, Vol. 28, No. 19

Publication 15.

Site-Directed DNA Crosslinking of Large Multisubunit Protein-DNA Complexes

Methods in Molecular Biology

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REFERENCES

References available on request.

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