Manager Assistant

Curriculum Vitae

Guangwu Zhu, B.S., M.S.

Sr. Research Specialist / Lab Manager

Personal Data

Sex: Male

Marital status: Married with one child

Address 116W University PKWY APT 1216 Baltimore, MD 21210 Telephone 1-443-***-****

E-mail ****.**@*****.***

Personal Statement

I have a strong interest in working in a life science research setting, which started more than a decade ago when I was first involved in a project studying genetic alterations in thyroid cancer. This initial interest encouraged me to pursue part-time (while taking care of my family) but formal graduate studies in biochemistry, molecular biology, and bioengineering sciences at the China Ocean University, Qingdao, China to prepare for a future life science research-related job/career. After coming to the Laboratory for Cellular and Molecular Thyroid Research at the Department of Medicine of Johns Hopkins University School of Medicine in 2013, I had the opportunity to focus on the training, study and research in human disease-related field, using thyroid cancer as a model, which led to my successful graduation of Master degree graduate studies. During this six years at Johns Hopkins, I joined the lab team to participate in several projects exploring the genetic alterations, particularly BRAF and TERT promoter mutations and their aberrant pathway signaling, to understand the molecular mechanism and the genetic-clinical outcome relationships in thyroid cancer. I have coauthored 17 high-impact research publications on thyroid cancer in prestigious scientific journals, such as Journal of Clinical Oncology (JCO; IF=26), Journal of National Cancer Institute (JNCI, IF=11), JAMA Oncology (IF=20), Nature Communications (IF=12), and other journals. This includes a recent research article published in JCO (IF=26) on which I was a first author. While working on my research studies in the lab at Hopkins, I also functioned as a Research Assistant, responsible for a variety of routine laboratory supportive and preparatory duties. These included, for example, ordering/purchases of lab supplies, equipment maintenance, technical trouble shouting/fixing, preparation for biohazard/radioactive safety check-ups and other regulatory inspections, animal management/maintenance, record/file documentation and keeping, planning/arrangement of lab meetings/activities, interaction/coordination with various departments/systems on the Johns Hopkins campus, and other functions to meet the needs of the lab. With my promotion to Lab Manager/ Sr. Research Specialist more than one year ago, my role in the lab has been extended in several directions, particularly at the lab and personal management levels. In the recent 1-2 years, in addition to continuing direct participation in research projects, a major part of my role has been to independently manage the laboratory’s normal daily operations to help the PI focus on his other professional commitments. As our PI is now moving elsewhere, I am now pursuing a rearrangement of my work setting by looking for a new job. I am very motivated to continue my job/career in a life science research-related setting, where I can continue to work as a lab staff/technician and use my strong training backgrounds and work experience to help the lab team to achieve success. Education/Training Experience

2007.07 ~2013.3 Graduate Study (part-time), Molecular Bioengineering and Biology, Ocean University of China, Qingdao, China

2013.1 – 2018.4 Graduate Study/Research Assistant in the Laboratory for Cellular and Molecular Thyroid Research, Division of Endocrinology, Diabetes and Metabolism, Department of Medicine, Johns Hopkins School of Medicine 2017.12 Completed thesis and full requirements for Master’s Degree in Molecular Bioengineering and Biology, Ocean University of China, Qingdao, China 2019.01 Received Diploma of Master’s Degree in Molecular Bioengineering and Biology, China Ocean University, Qingdao, China

Work Experience

2018.04 Lab Manager/Sr. Research Specialist, Johns Hopkins Department of Medicine Brief Summary After finishing my graduate studies on the research program in the Laboratory for Cellular and Molecular Thyroid Research at the Johns Hopkins Department of Medicine, I was promoted to the position of Lab Manager/ Research Specialist in the lab. In addition to my continued active participation in the research projects stated in the above Personal Statement, with the position of Lab Manager/Research Specialist, I have been also responsible for the management and organization of the daily operations of the lab. I use my broad research technical expertise to help team members to perform laboratory experiments and assays; trouble shot experimental issues; collect, process and maintain human cancer-related specimens; and maintain and culture cancer cell lines, Cell Biochemistry, perform DNA isolation, PCR, gene sequencing, Western blotting, microscopy, Animal care and many other experimental procedures. I play a major role in organizing, managing, maintaining, and monitoring daily operations of the lab. I provide day-day supervision and train junior postdoctoral fellows and students on the techniques and skills needed in this cancer research lab. I collaborate with the PI in overseeing the administrative and scientific implementation of the research protocols for research studies on cancer, particularly thyroid cancer. I function as a leader in maintaining and circulating research protocols among the team members, setting agendas and schedules for routine research-related meetings, processing laboratory protocols, and pursuing other routine duties in the lab, such as ordering supplies through the complex SAP. grcf system. Details of Some of my Research/Work Experience

2018.4- present

My role as a Lab Manager/Research Specialist at Johns Hopkins Department of Medicine:

Participate in laboratory research on a comprehensive thyroid cancer research program and develop and design research protocols to investigate molecular mechanisms of thyroid cancer and pursue clinical translation of the laboratory findings.

Use special skills and expertise to prepare and apply thyroid cancer cell lines, human thyroid tumors specimens, and animal thyroid tumor models to thyroid cancer research

Use a variety of laboratory tools, such as cell manipulations, cell inoculation to produce animal thyroid tumor models, molecular genetic tools, Western blotting, PCR, and genetic sequencing to conduct laboratory thyroid cancer search

Investigate genetic patterns in human thyroid tumors and perform genetic-clinical correlation analyses for diagnostic and prognostic clinical translational studies of thyroid cancer.

Organize, analyze and summarize the research data to prepare laboratory and scientific meeting reports on thyroid cancer research.

Help train and supervise junior postdoctoral fellows and students with the above skills and expertise for thyroid cancer research

Suggest ideas for new research projects.

Coordinates, organize and arrange regular scientific meetings for the team members as needed; prepares agendas, material for, and coordination of meetings with researchers on the thyroid research program in the lab.

Train junior research staff on job functions and performs quality assurance reviews for the scientific data.

Maintains detailed working knowledge of protocols

Designs spreadsheets and databases for tracking and management of research data

Data entry and management using database and other computer software

Ordering of lab supplies through the complex Johns Hopkins SAP system. 2013.4- 2018.4

Graduate Study/Research Assistant at Johns Hopkins University School of Medicine Working as a full-time Graduate Study Researcher/Research Assistant at the Laboratory for Cellular and Molecular Thyroid Research, Division of Endocrinology, Diabetes and Metabolism, Johns Hopkins School of Medicine, on thyroid cancer research projects, with a particular focus on the study of BRAF and TERT promoter mutations in thyroid cancer for their molecular mechanistic roles in thyroid tumor and for their genetic-clinical translational potential as diagnostic and prognostic markers and therapeutic targets. This research constituted the basis for my thesis dissertation and successful graduate study graduation for my Master degree in biological sciences. As a Research Assistant, my other role was to work as a Lab Research Assistant responsible for a variety of routine preparatory lab duties.

2007.6-2013.3 Biochemistry and Molecular Biology at China Ocean University Participating in a genome research project and systemic training in organism genome sequencing and bioinformatics analysis; prepared and performed basic experimental procedures.

Self-estimated Personal Characteristics

Self-confidence, optimistic, open-minded, strong sense of responsibility and high ethical and professional standards, strong self-learning ability and high efficiency, hardworking, creative and critical thinking, strong capability of problem solving, well organized and skillfully planned, humble and friendly. Other Skills

Excellent English language

Excellent computer skills in hardware maintenance and use of common Software, such as the Window Office system, etc.

Professional Society Membership

2019 Advance Thyroid Professional (ATP) Member,

The American Thyroid Association

Publications

1, Wang Y, Hou P, Yu H, Wang W, Ji M, Zhao S, Yan S, Sun X, Liu D, Shi B, Zhu G, Condouris S, Xing M. High prevalence and mutual exclusivity of genetic alterations in the PI3K/Akt Pathway in thyroid tumors. J Clin Endocrinol Metab. 2007; 92:2387-2390.

(Citation = 166; IF = 5.789)

2, Wang Y, Ji M; Wang W; Miao Z, Hou P, Chen X, Xu F, Zhu G, Sun X, Li Y, Condouris S, Liu D, Yan S, Pan J, Xing M. Association of the T1799A BRAF mutation with tumor extrathyroidal invasion, higher peripheral platelet counts, and over-expression of platelet-derived growth factor-B in papillary thyroid cancer. Endocrine-related cancer 2008; 15(1), 183-190. (Citation = 47; IF = 5.331)

3, Liu X, Qu S, Liu R, Sheng C, Shi X, Zhu G, Murugan AK, Guan H, Yu H, Wang Y, Sun H, Shan Z, Teng W, Xing M. TERT Promoter mutations and their association with BRAF V600E mutation and aggressive clinicopathological characteristics of thyroid cancer. J Clin Endocrinol Metab. 2014;99: E1130-6. (Citation = 192; IF = 5.789) 4, Xing M, Liu R, Liu X, Murugan AK, Zhu G, Zeiger MA, Pai S, Bishop J. BRAF V600E and TERT promoter mutations cooperatively identify the most aggressive papillary thyroid cancer with highest recurrence. J Clin Oncol 2014;32:2718-2726 (Received an editorial in J Clin Oncol 2014;32:2683-4) (Rated as a continued top-cited paper of the journal in 2017) (Citation = 395 ; IF =26.303)

5, Shi X, Liu R, Qu S, Zhu G, Bishop J, Liu X, Sun H, Shan Z, Wang E, Luo Y, Yang X, Zhao J, Du J, El-Naggar AK, Teng W, Xing M. Association of TERT promoter mutation 1,295,228 C>T with BRAF V600E mutation, older patient age, and distant metastasis in anaplastic thyroid cancer. J Clin Endocrinol Metab. 2015;100(4):E632-7.

(Citation = 61; IF =5.789)

6, Liu D, Shen X, Zhu G, Xing M. REC8 is a novel tumor suppressor gene epigenetically robustly targeted by the PI3K pathway in thyroid cancer. Oncotarget. 2015;6(36):39211-24.

(Citation = 36; IF = 5.168)

7, Cheng W, Liu R, Zhu G, Wang H, Xing M. Robust thyroid gene expression and radioiodine uptake induced by simultaneous suppression of BRAF V600E and histone deacetylase in thyroid cancer cells. J Clin Endocrinol Metab. 2016;101(3):962-971.

(Citation = 14; IF = 5.789)

8, Liu R, Bishop J, Zhu G, Zhang T, Ladenson PW, Xing M. Mortality risk stratification by combining BRAF V600E and TERT promoter mutations in papillary thyroid cancer: genetic duet of BRAF and TERT promoter mutations in thyroid cancer mortality. JAMA Oncol. 2017; 3(2): 202-208 [Received an Editorial in JAMA Oncol. 2017, http://jamanetwork.com/journals/jamaoncology/article-abstract/2546652); featured in Nat Rev Clin Oncol. 2016;13:652; also featured in MEDPageToday— https://www.medpagetoday.com/meetingcoverage/ata/48395] (Citation =89; IF=20.31) 9, Zhang T, Shen X, Liu R, Zhu G, Bishop J, and Xing M. Epigenetically upregulated WIPF1 plays a major role in BRAF V600E-promoted papillary thyroid cancer aggressiveness. Oncotarget 2017;8(1):900-914. (Citation = 5; IF = 5.168) 10, Wang F, Yu X, Shen X, Zhu G, Huang Y, Liu R, Viola D, Elisei R, Puxeddu E, Fugazzola L, Colombo C, Jarzab B, Czarniecka A, Lam AK, Mian C, Vianello F, Yip L, Riesco-Eizaguirre G, Santisteban P, O’Neill CJ, Sywak MS, Clifton-Bligh R, Bendlova B, Sýkorová V, Wang Y, Liu S, Zhao J, Zhao S, Xing M. The prognostic value of tumor multifocality in clinical outcomes of papillary thyroid cancer. J Clin Endocrinol Metab. 2017; 102 (9): 3241-3250. (Citation = 22; IF = 5.789) 11, Huang Y, Qu S, Zhu G, Wang F, Liu R, Shen X, Viola D, Elisei R, Puxeddu E, Fugazzola L, Colombo C, Jarzab B, Czarniecka A, Lam AK, Mian C, Vianello F, Yip L, Riesco-Eizaguirre G, Santisteban P, O'Neill CJ, Sywak MS, Clifton-Bligh R, Bendlova B, Sýkorová V, Xing M. BRAF V600E mutation-assisted risk stratification of solitary intrathyroidal papillary thyroid cancer for precision treatment. J Natl Cancer Inst. 2018;110

(4):362-370. (Highlighted in Johns Hopkins Research Output— https://jhu.pure.elsevier.com/en/publications/braf-v600e-mutation-assisted-risk- stratification-of-solitary-intr) (Citation = 17; IF =11.238) 12, Shen X,* Zhu G,* Liu R,* Viola D, Elisei R, Puxeddu E, Fugazzola L, Colombo C, Jarzab B, Czarniecka A, Lam AK, Mian C, Vianello F, Yip L, Riesco- Eizaguirre G, Santisteban P, O'Neill CJ, Sywak MS, Clifton-Bligh R, Bendlova B, Sýkorová V, Xing M. Patient age-associated mortality risk is differentiated by BRAF V600E status in papillary thyroid cancer. J Clin Oncol. 2018;36(5):438-445 [Received an Editorial by Haymart MR, J Clin Oncol. 2018;36(5):433-434] (Highlighted in Johns Hopkins Research Output— https://jhu.pure.elsevier.com/en/publications/patient-age-associated-mortality- risk-is-differentiated-by-braf-v) (*Equal first authors) (Citation = 30 ; IF = 26.303 ) 13, Liu R, Zhang T, Zhu G, Xing M. Regulation of mutant TERT by BRAF V600E/MAP kinase pathway through FOS/GABP in human cancer. Nat Commun. 2018;9(1):579. doi: 10.1038/s41467-018-03033-1. (Highlighted in Johns Hopkins Research Output— https://jhu.pure.elsevier.com/en/publications/regulation-of-mutant-tert-by-braf- v600emap-kinase-pathway-through) (Citation = 24; IF = 12.353) 14, Wang F, Zhao S, Shen X, Zhu G, Liu R, Viola D, Elisei R, Puxeddu E, Fugazzola L, Colombo C, Jarzab B, Czarniecka A, Lam AK, Mian C, Vianello F, Yip L, Riesco-Eizaguirre G, Santisteban P, O’Neill CJ, Sywak MS, Clifton-Bligh R, Bendlova B, Sýkorová V, Wang Y, Xing M. BRAF V600E confers male sex disease-specific mortality risk in patients with papillary thyroid cancer. J Clin Oncol. 2018; 36(27):2787-2795.

[Featured in Healio’s In The Journals Plus (ITJ+)— https://www.healio.com/hematology- oncology/head-neck-cancer/news/in-the-journals/%7Bd77c976e-0721-4b86-a065- 43da92ab0615%7D/men-with-braf--mutated-papillary-thyroid-cancer-may-have-worse- survival-outcomes]; [Also featured in CancerNetwork— http://www.cancernetwork.com/thyroid-cancer/male-sex-ups-mortality-risk-braf-v600e- papillary-thyroid-cancer] (Citation = 5 ; IF = 26.303 ) 15, Liu Z, Shen X, Liu R, Zhu G, Huang T, Xing M. Stage II differentiated thyroid cancer is a high-risk disease in patients <45/55 years old. J. Clin Endocrinol Metab. 2019 (accepted for publication). (Citation = N/A; IF = 7.557) 16, X Shen, G Zhu, R Liu, D Viola, R Elisei, E Puxeddu, L Fugazzola, C Colombo, B Jarzab, A Czarniecka, AK Lam, C Mian, F Vianello, L Yip, G Riesco- Eizaguirre, P Santisteban, EJ O'Neill, MS Sywak, R Clifton-Bligh, B Bendlova, V Sykorova, M Xing, Iuliana Bobanga. Endocrine Surgery Review Patient Age-Associated Mortality Risk Is Differentiated by BRAF V600E Status in Papillary Thyroid Cancer. Oncology,2018; 36(5):438-445. (Citation = N/A; IF =2.600) 17. Liu J, Liu R, Shen X, Zhu G, Li B, Xing M. The Genetic Duet of BRAF V600E and TERT Promoter Mutations Robustly Predicts the Loss of Radioiodine Avidity in Recurrent Papillary Thyroid Cancer. J Nuc Med 2019 (Accepted, in press).

(Citation = 1; IF =7.354)

(Total citations: 1104; Total impact factors: 182.841) Work Presented at International Meetings

Tan J, Shen X, Liu R, Zhu G, Rooper L and Xing M. RASAL1 is a master gene in the development of thyroid hyperplasia/multinodular goiter—evidence from a novel Rasal1 knockout mouse model. The 88th Annual Meeting of American Thyroid Association (ATA), Washington, D. C., October 3-7, 2018. I also contributed substantially to the research work of the lab presented at the ATA’s annual meetings in 2014, 2015, 2016 and 2017

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