Plant Manager

Dr. RAMACHANDRA Nagaraju *************@*****.***

#****, *

th

Cross, Gayathrinagar, 11.05.1979

Bangalore, India Married

+919********* Indian

Objective

Desire for an industrial scientific career in an intellectually stimulating environment that empowers my scientific expertise and management skills to have a key impact on the success of an organization and personal growth. Professional experience

Senior Scientist, Team Lead Yeast Biology Group, Premas Biotech, Manesar, IN July ’17 – August’19 Scientist, Team Lead Yeast Biology Group, Premas Biotech, Manesar, IN April ’15 – June’17 Activities:

• Leading Premas yeast biology group of maximum 7 team members

• Driving the Premas yeast biology team into customer focused and time bound delivery mindset

• Project proposal generation and its techno-economical feasibility analysis

• Project strategy design, its execution from DNA to protein in yeast system, such as Saccharomyces cerevisiae and Pichia pastoris

• Molecular biology, expression of secretary, soluble cytosolic and transmembrane recombinant proteins and its analysis in yeast expression host system

• High-throughput expression screening for the selection of best expression clones

• Expression optimization with varying pH, temperature, media composition and additives at small scale and scale-up cultures in shake flask

• Development of yeast strains by adopting microbial genetic manipulations such as knockout and site-directed mutagenesis in the model organism S.cerevisiae and P.pastoris

• Generation of various in-house episomal and integrative yeast expression vectors by recombinant engineering and homologous recombination.

• Efficient Team Management and Project Management

• Efficient customer communication with timely results and project report update

• Adopting data and literature based interim strategies to understand intellectual insights of the project to overcome the technical constraints of the project

• Inter-team co-ordination for upstream and downstream processes for the delivery

• Knowledge sharing, discussion of results, trouble shooting, Day-to-Day planning and monitoring of the executors

• Adoption of GLP, generation and timely revision of SOPs

• An active interview panel member for recruitment of candidates for Team Premas Biotech

• Premas internal auditor team member for audit surveillance as per ISO 9001:2015 Acquisitions:

• Sense of priority, autonomy, data interpretation, teamwork and customer interaction

• Transition and adaptation from academic research to delivery based industrial research

• Exposure to drive the projects from Gene to Investigational new drug (IND) discovery

• Oral Insulin Drug development: Developed a P.pastoris clone for secretary expression of a recombinant plant protease inhibitors to substitute purified plant protein, to be used as an API in Oral insulin pill

• Involvement in its process development for yield enhancement to up to 3 g/L and inter-team co-ordination for purification optimization

• Co-ordination for the repeat delivery of active purified recombinant protease inhibitor protein in from fermenter batches for Oral insulin pre-clinical studies

• Recombinant protease inhibitor protein Clone development report generation for IND and FDA filing

• P. pastoris Research Cell bank (RCB) validation studies as per ICH 5B and ICH 5D guidelines

• Patent for the Recombinant protease inhibitor protein production from P. pastoris Clone is under process

• Successfully delivered several query recombinant proteins from plant and human origin from yeast expression hosts

• Cloning, expression and co-ordination for the purification of human recombinant ion channel, human peptide, viral glycoprotein and vaccine conjugate proteins in yeast host. Its functional studies, expression and purification optimization at 1L scale up is in process

• High-throughput secretary expression screening and its analysis of 38 recombinant proteins in P.pastoris within bound limited time period

• Establishment of Yeast biology platform by introducing bench protocols for yeast based research

• Establishment of an independent and efficient Yeast biology group in Premas Biotech

• Project management from proposal, gene synthesis, initiation, expression screening to scale-up, process development, purification and delivery

• Genetic modification of Yeast for enhanced secretary expression of recombinant proteins and yield enhancement

• Upkeep the customer expectations to procure repeat order or more projects from the same customer Post-doctoral fellow, University of Fribourg, CH July 2013 – August 2013 Ph.D. in Biochemistry, Unit of Biochemistry and University of Fribourg, CH May 2007 – July 2013 Acquisitions:

• Planning and rational organization of work, adaptation and contact with persons of different nationality

• Development of yeast strains by adopting microbial genetic manipulation

• Synthetic Genetic Array analysis (SGA) of yeast essential and non-essential genetic mutants

• ER Membrane protein topology determination and in vitro biochemical assays for ceramide synthases from yeast and higher eurkaryotes

• Survey of observance of rules regulating the handling of radioactive isotopes and its de-contamination

• Excellent computer skills (Excel, Power point, Word) and solid understanding and extensive utilisation of bioinformatics tools

• Independence, flexible, sense of responsibility, ability to make decisions, to balance risks and to reduce the probability of failures

• Methodologies: Yeast genetics, Molecular biology, Immunoprecipitation, membrane protein modification by chemical conjugation, extraction/analysis of membrane lipids and proteins, and metabolic labelling of lipids Research Assistant Sept. 2005 – April 2007

Dept. of Biochemistry and Molecular Biophysics Unit, IISc, India. Prof. HS Savithri, Prof MRN Murthy and Emeritus Prof. N Appaji Rao Acquisitions:

• Bacterial genetics, molecular biology and microbiology

• Protein chemistry, structure-functional relationship studies and protein crystallisation

• Recombinant protein expression, its classical and tag-mediated protein purification methods such as Affinity, Ion Exchange, Hydrophobic Interaction, Size Exclusion chromatography

• Enzymology, crystallization and gene knock out studies of Diaminopropionate ammonia lyase. Model organisms: Salmonella typhimurim LT2 and E.coli K12

• Methylenetetrahydrofolate reductase gene polymorphisms studies in clinical samples of cancer patients Studies and diplomas

University of Fribourg, Department of Biology, Switzerland May 2007 - July 2013 Ph.D. in Biochemistry:

Thesis: “Membrane topology and active site orientation studies of Yeast Alkaline Ceramidase YPC1” Vellore Institute of Technology, Tamil Nadu, India Sept. 2003 –Aug. 2005 Master of Technology (Biotechnology)

Rajiv Gandhi University of Health and Science, Karnataka, India Sept. 1997 – Feb. 2002 Bachelor of Pharmacy

Patent

“RECOMBINANT PROTEASE INHIBITOR-CONTAINING COMPOSITIONS, METHODS FOR PRODUCING SAME AND USES THEREOF”, Kajal Arora, Ramachandra Nagaraju, Nupur Mehrotra Arora, Prabuddha Kumar Kundu and Miriam Kidron. Provisional application for Letters Patent of the United States, filed in June 11, 2018 of serial number 62/683,061. Achievements

• Technical Leap of the Year 2017, Premas Biotech

• Best Scientific turnaround of the Year 2016, Premas Biotech

• GATE Qualified 2003

Certification Courses sponsored by Premas Biotech

• “Internal Auditing for QMS based on ISO 9001:2015” by TUV SUD South Asia Pvt. Ltd., Gurugram, IN, March 2019

• “Basic Good Manufacturing practices” by, Advanced Regulatory Services Ltd. (ADRES), Manesar, IN, Nov. 2017

• “Data Integrity and Source Data Verification” by ADRES, Manesar, IN, Nov. 2017

• “Deviations, Change Control, Out of Specification, Investigation and Root Cause Analysis” by ADRES, Nov. 2017 Seminars attended

• “Competency Based Interviewing Skills” by TALENT SHAPERS, Manesar, IN, Nov. 2017

• “Management Effectiveness” by Mr. Suresh Mohan Semwal, POSSIBLERS, Delhi, IN, May 2017

• “Yeast system biology workshop”, Fribourg, CH, June 2012

• “Membrane dynamics in physiology and disease”, Basel, CH, June 2012

• EUROFED lipid conference, “Microbial Lipids: Diversity in structure and function”, Bern, CH, May 2012

• “Swiss yeast meeting”, Fribourg, CH, September 2010

• “Yeast Genetics and Molecular Biology meeting”, Vancouver, CA, August 2010

• “Bioinformatics”, Fribourg, CH, September 2009

• International conference on Biotechnology (ICOB), VIT, Tamil Nadu, IN, Feb. 2008 Posters

• Kajal Arora, Ramachandra Nagaraju, et al., “Multi-pronged approach for successful over expression of a plant sourced protein with therapeutic indications” PEGS 2016, Boston

• Ramachandra Nagaraju, et al. : “Molecular and functional characterization of S. Cerevisiae YPC1p; An yeast alkaline ceramidase and its topological orientation across ER”, YGMB meeting, August 2010

• Nagaraju Ramachandra, et al.: “Identification of the role of a novel enzyme, Diaminopropionate ammonia lyase

(DAPAL) in branched chain amino acid biosynthetic pathway in Salmonella typhymurium LT2 by Gene knockout Studies”, ICOB meeting, Feb. 2007. Awarded Best Poster Publications

1. Ramachandra Nagaraju, Avram Hershko, et.al., “Recombinant Protease Inhibitor Enhances Oral Insulin Pharmacodynamics in Pigs”, American Diabetes Association, Diabetes 2018 2. Ramachandra N and Conzelmann A, “Membrane Topology of Yeast Alkaline Ceramidase YPC1” Biochem J. 2013. 3. Bochud A, Ramachandra N, Conzelmann A. “Adaptation of low-resolution methods for the study of yeast microsomal polytopic membrane proteins: a methodological review”, Biochem Soc Trans. 2013 4. Kalyani JN, Ramachandra N, Kachroo AH, Mahadevan S, Savithri HS. “Functional analysis of the genes encoding diaminopropionate ammonia lyase in Escherichia coli and Salmonella enterica serovar typhimurium” J.Bacteriology, 2012.

5. Sadananda Adiga MN, Chandy S, Ramachandra N, Appaji L, Aruna Kumari BS, Ramaswamy G, Savithri HS, Krishnamoorthy L. “Methylenetetrahydrofolate reductase gene polymorphisms and risk of acute lymphoblastic leukemia in children”,Indian J Cancer. 2010.

6. Chandy S, Sadananda Adiga MN, Ramachandra N, Krishnamoorthy S, Ramaswamy G, Savithri HS, Krishnamoorthy L. “Association of Methylenetetrahydrofolate reductase gene polymorphisms & colorectal cancer in India”, Indian J Med Res. 2010.

7. Rajaram V, Prasad K, Ratna Prasuna P, Ramachandra N, Bharath SR, Savithri HS, Murthy MR. “Cloning, purification, crystallization and preliminary X-ray crystallographic analysis of the biosynthetic N-acetylornithine aminotransferases from Salmonella typhimurium and Escherichia coli”,Acta Crystallogr Sect F Struct Biol Cryst Commun. 2006.

Languages

English, Kannada and Hindi: Excellent written and spoken Tamil and Telugu: Excellent spoken

References

Available on request

Bangalore, October, 2019

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