Joshua Z Johnson
Objective: I am a biologist with 4 years of experience in molecular and cell biology with extensive cell culture experience using CRISPR-Cas9 in human stem cells who wishes to work in an industrial setting.
LABORATORY TECHNIQUES & SKILLS
PCR, restriction digest, site-directed mutagenesis, southern blot analysis (radioactive probes), Western blot analysis, SDS-PAGE, DNA extraction, ELISA, mammalian cell culture (human embryonic stem cells, human fibroblasts, cell lines such as HEK293T and HeLa), T7 endonuclease I assays aimed to determine genome targeting efficiency, fluorescent in situ hybridization, lentivirus production and cell transduction, qPCR and qRT-PCR, differentiation of human embryonic stem cells into fibroblasts, neurons, and cardiomyocytes; Genome editing using CRISPR-Cas9 system (successfully applied in human embryonic stem cells), and fluorescent microscopy.
La Escuelita / Teach for America, Oakland, CA June 2017 – June 2019
Middle School Science Teacher / Corps. Member
La Escuelita is a Title I school, due to high concentration of poverty among students
University of California, Berkeley, CA Aug. 2013-May 2017
Master of Arts Degree, Department of Molecular and Cell Biology
Studied cancer biology in the lab of Dirk Hockemeyer by using genome editing techniques in a human stem cell model system, which resulted in authorship on two peer-reviewed papers and primary authorship on a stem cell model system review
Taught a variety of undergraduate course within the biology major, including laboratory courses and lecture discussion sections
University of South Carolina Upstate, Spartanburg, SC Aug. 2009-May 2013
Bachelor of Science in Biology
GPA: 3.95, Summa Cum Laude
President, Chemistry Club (largest science club at the university)
Vice President, USC Upstate Lion’s Club
Supplemental Instructor (led discussion sections, and coached and mentored fellow instructors)
Vogan JM, Zhang X, Yoxumans DT, Regalado SG, Johnson JZ, Hockemeyer D, Collins K. Minimized human telomerase maintains telomeres and resolves endogenous roles of H/ACA proteins, TCAB1, and Cajal bodies. eLife. 2016.
Chiba K, Johnson JZ, Vogan JM, Wagner T, Boyle JM, Hockemeyer D. Cancer-associated TERT promoter mutations abrogate telomerase silencing. eLife 2015.
Johnson JZ and Hockemeyer D. Human stem cell-based disease modeling: prospects and challenges. Current Opinion in Cell Biology. 2015.