“Curriculam vate or resume”

First Name : Shamim Akhter, Last Name : Ansari

Profession : Medical Laboratory Technologist”

( ICAS- CANADA )

Total Professional Experience 22 years

& Instructor for New Trainees Students Bachelor of Science,

Basic Details :-

1st Column : Address:-

Pathology Department ( Micro – Section )

King Fahad Hospital, Ministry of Health

General Directorate of Health Affairs,

Po. Box- 204, Zip Code :- 11074,

Al- Baha, Saudi Arabia

Phone ( Mobile ) :- +966*********

Tele.No : - (+966 ) 17725-4000, EXT : 3795,3832,4500

Email ID :- ada2xp@r.postjobfree.com

Personnel Information :-

Date of Birth : 8th Day of April, 1972

Marital Status : Married with Mrs. Sajda Khatoon

( Middle School Urdu Teacher ),

Bachelore of Education ( Arts )

Kin Chin Status : Daughter ( 1 ), Son ( 2 )

Religion : Muslim, Whorship – Islam

Language Known : English, Hindi, Urdu

2nd Column:-

Twitter :- @saj44a

FaceBook ID : ada2xp@r.postjobfree.com

Linkedin : ada2xp@r.postjobfree.com

Summary ( Optional )

“Biography of Professional Experience”

After awarded Diploma Certificate in the year August 1997, We joined Dewan Diagnostic Center, New Delhi ( India ) as a Medical Lab. Technician in this Diagnostic Centre without Pathologist We had been done the Routine Hematological Test, Serological Test, Urinalysis, Immunological Test & Parasitological Test, Routine Blood Banking Test, in the morning Shift, ( 8 AM to 12 PM ).

In the year September 1997, I got the Job as a Medical Lab. Technician in Dr. D.K. Gulati ( Path - Lab ) New Delhi, ( India ) in this Pathology – Lab. Routine Investigation of Biochemistry, Hematology, Microbiology, Parasitology, Serology & Immunological Test & Blood Banking Test done it under Supervision of Pathologist, in the Evening Shift ( 1PM to 8 PM ),

“Migrate to Saudi Arabia November, 2002 “

In the year November 2002, I got Selection in the Ministry of Health, Government of Saudi Arabia by Delegation of Saudi Arabian Government, Organized by Saudi Embassy New Delhi, India as a Medical Laboratory Technician.

I had been joined 11th Day of November 2002, General Directorate of Health affairs at Al- Baha, King Fahad Hospital, Ministry of Health in the Blood Banking Section as a Part of Pathology Department, in this Section I had been worked Three Shift duty ( Morning, Evening & Night Shift ) 5 years, We had been done Blood Grouping, Cross – Matching, Component Preparation, & General Routine Statistics, & as a Phlebotomist in Blood Donor Section,

In the year July 2007, By the Virtue of Executive Medical Director & Chief of Pathology, I awarded Best Performance Certificate for Good Moral Character & Best Activity in the Pathology Department, King Fahad Hospital, Al- Baha, ( KSA ).

In the year October, 2007 I got Departmental Transfer from Blood Bank to Microbiology Section, At Present in this Section I am giving Services Mycobacterium Tuberculosis Analysis ( e.g. All Tuberculosis Samples Set Up, Slides Preparation, Staining by two method Ziehl Neelson Stain & Auramin Stain, Reading & Releasing results & Tuberculosis Sensitivity Processing & releasing results ) All types of Media Preparation, Reagent Preparation, Parasitological Examination Test, Microbiological Routine & Special Examination Test Set Up & Identification of Ova & Parasites & Stool Special Examination Test, etc.

“Academic & Professional Education”

School # 1

School Name, major, minor, Degree

Bihar Intermediate Education Council, Bihar ( India )

Date from : Date to :

January 1st, 1979 June 1st, 1991.

Description :-

Higher Secondary School Examination- June, 1991

10+2 ( Biology + Physics & Chemistry + Zoology & Botany )

Optional ( Mathematics )

Institution # 3

Institute of Para – Medical Technology, New Delhi ( India )

Date from : Date to :

May 1st, 1995 August 27th, 1997

Description :

( DMLT ) Diploma in Medical Laboratory Technology – 1997,

Technical University # 4

Punjab Technical University, Punjab ( India )

Date from : Date to

August 1st, 2008 September 30th, 2010

Description :

Distance Education Courses

B.Sc ( MLT ), Bachelor of Science – 2010,

University # 2

DR. BR Ambedkar Bihar University, Bihar ( India )

Date from : Date to :

September 1st, 1991 May 30th, 1994

Description :

Bachelor of Science ( Chemistry Honors ) – 1994,

Main Subject –( Chemistry), Subsidiary Subject – (Zoology + Botany ).

Failure in Final Examination,

Projects

Saudi Arabian Health Commission # 5

Saudi Commission for Health Specialties, Saudi Arabia

Date from : Date to

December 30th, 2003 September 24th, 2019

Description : -

Diploma in Medical Laboratory Technology Certificate Equalized from Saudi Commission for Health Specialties by Examination of Council, Saudi Arabia,

“Technical Skills”

Health Care Skill #1

Saudi Heart Association, Saudi Arabia

Date from : Date to

May 19th 2008, May 19th, 2019

Description :

BLS ( Basic Life Support Course ),

In this course when we are found Unconscious Patient in the Hospital or Outside the Hospital giving CPR (Chest Pulmonary Recitation), for adults & Children, Old aged ( Male & Female ) .

Computer Skill # 2

Information Technology ( IT-Department)

King Fahad Hospital, Al- Baha, Saudi Arabia

Date from : Date to:

March 1st, 2003 Till today

Description:-

In Computer Microsoft Words Doc. Microsoft Excel Doc. Pathological Test addition, & Modification in Pathology Department, General Departmental Statistics Preparation, Protocol Maintenance in the Department,

“Experience in the Professions”

Company # 1

Line 1 ( Position ) Line 2 : Company / Department

Medical Laboratory Technician Dewan Diagnostic Center

Date From : Date to :

August 25th, 1997, September 30th, 2002

Experience & Responsibility :-

Dewan Diagnostic Center,New Delhi ( India )

We had worked as a Medical Laboratory Technician in this Clinical Pathology Laboratory, We performed Heamatological Test Complete Blood Count ( CBC ), Differential Blood Count ( DLC ), Urine Analysis, Stool Analysis, Serological Test, Venereal Disease Research Laboratory Test ( VDRL ), C-Reactive Protein ( CRP ), Rheumatoid Factor Test ( RA- Factor ), Manual Routine Kit Test ( for HIV Test ), & Phlebotomist for Blood Collection for Special Blood Examination Test,

Company # 2

Line 1 ( Position ) Line 2 : Company/ Department

Medical Laboratory Technician Dr. DK. Gulati Path- Lab

Date From : Date to :

September 1st, 1997 October 30th, 2002

Experience & Responsibility :-

Dr. DK – Gulati ( Path – Lab )New Delhi ( India )

We had been worked as a Medical Laboratory Technician in this Clinical Pathology Laboratory We had been performed Hematological Test Complete Blood Count ( CBC ), & Differential Blood Count ( DLC ), Urine Analysis, Stool Analysis, Serological Test, Venereal Disease Research Laboratory Test ( VDRL ), C-Reactive Protein ( CRP Test ), Rheumatoid Factor Test ( RA- Factor Test ), Manual Routine Kit Test ( for HIV Test ), & Phlebotomist for Blood Collection for Special Blood Examination Test,

During that Period I had been handle different Laboratory Equipment as in RA – 50, Erba Chem – 5, Bio Chemistry Analyzer, Photo Flame Meter, Calorimeter, PhotoChem, Coulter Hematology Analyzer etc,

Company # 3,

Ministry of Health ( SAUDI ARAbia )

Line 1 ( Position ) Line 2 ( Company/ Department )

Medical Laboratory Technologist ( Pathology Department )

Ministry of Health,

King Fahad Hospital,Al-Baha KSA

Date from : Date to :-

November, 11th Day, 2002 Till Today

“Blood Bank Section”

Experience & Responsibility :- ( 5 years ),

I had been joined 11th Day of November 2002, General Directorate of Health affairs at Al- Baha, King Fahad Hospital, Ministry of Health in the Blood Banking Section as a Part of Pathology Department, in this Section I had been worked Three Shift duty ( Morning, Evening & Night Shift ) 5 years, We had been done Blood Grouping, Cross – Matching, Component Preparation, & General Routine Statistics, & as a Phlebotomist in Blood Donor Section,

‘Types of Blood Transfused”

Whole blood or blood with RBC, is transfused to patients with anaemia/iron deficiency. It also helps to improve the oxygen saturation in blood. It can be stored at 1.0 C-6.0 C for 35–45 days. Platelet transfusion, is transfused to those who suffer from low platelet count. This can be stored at room temperature for 5–7 days. Plasma transfusion is indicated to patients with liver failure, severe infections or serious burns. Fresh frozen plasma can be stored at a very low temperature of -25 C for up to 12 months.

Collection Blood Samples

The collection and processing of each blood product. "Whole Blood" (WB) is the proper name for one defined product, specifically unseparated venous blood with an approved preservative added. Most blood for transfusion is collected as whole blood. Autologous donations are sometimes transfused without further modification, however whole blood is typically separated (via centrifugation) into its components, with red blood cells (RBC) in solution being the most commonly used product. Units of WB and RBC are both kept refrigerated at 33.8 to 42.8 F (1.0 to 6.0 C), with maximum permitted storage periods (shelf lives) of 35 and 42 days respectively. RBC units can also be frozen when buffered with glycerol, but this is an expensive and time-consuming process, and is rarely done. Frozen red cells are given an expiration date of up to ten years and are stored at −85 F (−65 C).

The less-dense blood plasma is made into a variety of frozen components, and is labeled differently based on when it was frozen and what the intended use of the product is. If the plasma is frozen promptly and is intended for transfusion, it is typically labeled as fresh frozen plasma. If it is intended to be made into other products, it is typically labeled as recovered plasma or plasma for fractionation. Cryoprecipitate can be made from other plasma components. These components must be stored at 0 F (−18 C) or colder, but are typically stored at −22 F (−30 C). The layer between the red cells and the plasma is referred to as the buffy coat and is sometimes removed to make platelets for transfusion. Platelets are typically pooled before transfusion and have a shelf life of 5 to 7 days, or 3 days once the facility that collected them has completed their tests. Platelets are stored at room temperature (72 F or 22 C) and must be rocked/agitated. Since they are stored at room temperature in nutritive solutions, they are at relatively high risk for growing bacteria.

Storage and management,

Outline blood storage is 42 days or 6 weeks for stored packed red blood cells (also called "StRBC" or "pRBC"), by far the most commonly transfused blood product, and involves refrigeration but usually not freezing. There has been increasing controversy about whether a given product unit's age is a factor in transfusion efficacy, specifically on whether "older" blood directly or indirectly increases risks of complications. Studies have not been consistent on answering this question, with some showing that older blood is indeed less effective but with others showing no such difference; nevertheless, as storage time remains the only available way to estimate quality status or loss, a first-in-first-out inventory management approach is standard presently. It is also important to consider that there is large variability in storage results for different donors, which combined with limited available quality testing, poses challenges to clinicians and regulators seeking reliable indicators of quality for blood products and storage systems.

Transfusions of platelets are comparatively far less numerous, but they present unique storage/management issues. Platelets may only be stored for 7 days, due largely to their greater potential for contamination, which is in turn due largely to a higher storage temperature.

Blood Component Preparation & Storage

The preparation of blood components involves several steps of manufacturing, which begin as soon as the donation of the blood product is complete. Donations may be either whole blood or aphaeresis. This chapter reviews the processes for manufacturing, storage conditions, and the required quality control (QC) for red blood cells, platelets, plasma, and Cryoprecipitate AHF. Modifications to the products, such as irradiation, leukoreduction, and pathogen inactivation are discussed. Additionally, granulocyte collections are reviewed, as they are an infrequent but important blood product for severely neutropenic patients with infections. Throughout the chapter, several scenarios which mimic situations one may see in either a blood center or in the hospital are included.

Microbiology Section

Till today,

Summary ( Optional ),

In the year, July 1st, 2007 I had been got Departmental Transfer from Blood Bank to Microbiology Section, At Present ( Microbiology Section ) in this Section I am giving Services Mycobacterium Tuberculosis Analysis ( e.g. All Tuberculosis Samples Set Up, Slides Preparation, Staining by two method Ziel Nelson Stain & Auramin Stain, Reading & Releasing results & Tuberculosis Sensitivity Processing & releasing results ) All type of Media Preparation, All type of Reagent Preparation, Parasitological Examination Test, Microbiological Routine Test & Special Examination Test Set Up & Identification of Ova & Parasites & Stool Special Examination Test,

Mycological study of Fungus, 10% KOH Preparation for Fungus, Inoculation of Fungal material in the Media ( e.g. Saboured agar, Chloromphenicol Saboured agar),

MRSA Screening Test by BD MAX, Clostridium Defficile Toxin – A & B Antigent Test by BD Max, all other test We can run on BD Max,

PCR Test (Polymerization Chain Reaction Test) by Zen Expert for Mycobacterium Tuberculosis, & Nasal MRSA Screening Test.

Branches of Microbiology

The branches of microbiology can be classified into pure and applied sciences, or divided according to taxonomy, as is the case with bacteriology, mycology, protozoology, and phycology. There is considerable overlap between the specific branches of microbiology with each other and with other disciplines, and certain aspects of these branches can extend beyond the traditional scope of microbiology,

Bacteriology

Bacteriology is the branch and specialty of biology that studies the morphology, ecology, genetics and biochemistry of bacteria as well as many other aspects related to them. This subdivision of microbiology involves the identification, classification, and characterization of bacterial species. Because of the similarity of thinking and working with microorganisms other than bacteria, such as protozoa, fungi, and viruses, there has been a tendency for the field of bacteriology to extend as microbiology. The terms were formerly often used interchangeably. However, bacteriology can be classified as a distinct science.

Mycology

Mycology is the branch of biology concerned with the study of fungi, including their genetic and biochemical properties, their taxonomy and their use to humans as a source for tinder, medicine, food, and entheogens, as well as their dangers, such as toxicity or infection.

A Biologist specializing in mycology is called a mycologist.

Mycology branches into the field of physiopathology, the study of plant diseases, and the two disciplines remain closely related because the vast majority of plant pathogens are fungi.

Parasitology

Parasitology is the study of parasites, their hosts, and the relationship between them. As a biological discipline, the scope of Parasitology is not determined by the organism or environment in question but by their way of life. This means it forms a synthesis of other disciplines, and draws on techniques from fields such as cell biology, bioinformatics, biochemistry, molecular biology, immunology, genetics, evolution and ecology.

Virology

Virology is the study of viruses – submicroscopic, parasitic particles of genetic material contained in a protein coat – and virus-like agents. It focuses on the following aspects of viruses: their structure, classification and evolution, their ways to infect and exploit host cells for reproduction, their interaction with host organism physiology and immunity, the diseases they cause, the techniques to isolate and culture them, and their use in research and therapy. Virology is considered to be a subfield of microbiology or of medicine.

‘Immunology Section”

Immunological tests We had been performed widely used. Their areas of application include:

Bowel Cancer Screening: This test looks for the blood pigment hemoglobin, a sign of blood in stool. Blood in stool can be caused by various things, such as hemorrhoids, polyps or even bowel cancer.

Allergy tests: to detect antibodies against allergy-triggering substances like grass pollen or certain foods.

Detecting germs causing an infection: If it is thought someone has bacterial tonsillitis or scarlet fever, the test looks for Streptococcus bacteria. In the case of Lyme disease following a tick bite, there are tests that can detect the Borrelia bacteria that cause it, and there are tests that can detect the antibodies to Borrelia bacteria. Immunological tests can also be used to detect viruses. Examples include hepatitis C, HIV or HPV viruses. Pregnant women can have a blood test to find out whether they are protected from (immune to) toxoplasmosis.

Diagnosing Heart attacks and thrombosis: Shortly after a heart attack or if someone has thrombosis, higher levels of a certain protein are found in the blood. These can be detected using an immunological test.

Urine test: If sugar, blood, proteins or inflammatory cells are found in urine using this rapid test, it could be a sign of diabetes, a urinary tract infection or kidney damage.

Pregnancy test: Women can use this rapid test to find out whether their urine contains the "pregnancy hormone" Beta-hCG.

Rapid tests for drugs and medication: Immunological tests can also be used to look for recreational drugs such as cannabis, ecstasy and cocaine. Medical drugs that affect the central nervous system can also be detected in this way. These include sleeping pills (benzodiazepines), amphetamines and morphine.

Determining your Blood group: When blood transfusions are done, the person donating the blood and the person receiving the blood have to have the same blood group. Immunological tests can be used to determine the blood groups before a blood transfusion.

Immunological tests can also be used to diagnose congenital or acquired immune diseases, differentiate between different forms of rheumatoid arthritis, or monitor the progression of an existing medical condition, such as certain tumors (in prostate cancer the PSA levels in blood are monitored).

The antibody principle is also applied in doping tests, food hygiene tests, and tests for toxic substances.

‘Histo pathology Section”

Laboratories use a variety of methods to perform the vast range of tests needed for diagnosis, monitoring treatment and managing your health. Understanding the method used for a test provides a useful context for understanding your test results.

Laboratory methods are based on established scientific principles involving biology, chemistry and physics, and encompass all aspects of the clinical laboratory from testing the amount of cholesterol & glucose in your blood, to investigating your DNA, to growing infectious organisms and to observing cell integrity with a microscope. Pathology testing is one of the most regulated areas of medicine and stringent quality control systems are always in place. Test methods specifically define the procedures or processes that are to be used. The laboratory scientist carefully follows step-by-step procedures until the end product, a test result, is achieved.

Some methods are much more complicated and Labour-intensive than others and require varying degrees of expertise. Often, more than one method can be used to test for the same substance. A test may be performed by a different method in different Laboratories, a fact that is crucial when comparing test results. Therefore, the result can only be interpreted using the reference interval provided with the result to determine if there is an abnormality that needs to be followed up by the requesting Doctor.

Histopathology (or histology) involves the examination of sampled whole tissues under the microscope. Three main types of specimen are received by the pathology laboratory.

Specimens received by the pathology laboratory require tissue preparation then are treated and analyzed using techniques appropriate to the type of tissue and the investigation required. For immediate diagnosis during a surgical procedure a frozen section is performed

1.Larger specimens include whole organs or parts thereof, which are removed during surgical operations. Examples include a uterus after a hysterectomy, the large bowel after a colostomy or tonsils after a tonsillectomy.

2.Pieces of tissue rather than whole organs are removed as biopsies, which often require smaller surgical procedures that can be performed whilst the patient is still awake but sedated. Biopsies include excision biopsies, in which tissue is removed with a scalpel (e.g. a skin excision for a suspicious mole) or a core biopsy, in which a needle is inserted into a suspicious mass to remove a slither or core of tissue that can be examined under the microscope (e.g. to investigate a breast lump).

3.Fluid and very small pieces of tissue (individual cells rather than groups of cells, e.g. within fluid from around the lung) can be obtained via a fine needle aspiration (FNA). This is performed using a thinner needle than that used in a core biopsy, but with a similar technique. This type of material is usually liquid rather than solid, and is submitted for cytology rather than histology & Cytopathology.

Specimens received by the pathology laboratory require initial tissue preparation, then are treated and analyzed using techniques appropriate to the type of tissue and the investigation required.

For immediate diagnosis during a surgical procedure, which may influence the type of surgery being performed, a frozen section is done.

Cytology Section

Cytology is the study of individual cells and Cytopathology is the study of individual cells in disease. Sampled fluid/ tissue from a patient is smeared onto a slide and stained (see techniques). This is then examined under the microscope by the anatomical pathologist to look at the number of cells on the slide, what types of cells they are, how they are grouped together and what the cell details are (shape, size, nucleus etc). This information is useful in determining whether a disease is present and what is the likely diagnosis.

Cytology is most often used as a screening tool to look for disease and to decide whether or not more tests need to be performed. An example of screening would be the investigation of a breast lump. In combination with examination by the clinician and imaging tests, a needle aspirate of the lump submitted for cytology will show whether the breast cells are suspicious for cancer or look bland/ benign. If they look suspicious, a core biopsy with a larger needle may be performed which takes more tissue, allowing for a definitive diagnosis to be made before deciding what type of surgery is required (local removal of the lump or removal of the whole breast).

Sampling Techniques in Cytology

Exfoliative Cytology

This is the analysis of cells that are shed from body surfaces. Examples include the lining cells of the uterine cervix (mouth of the womb) and of the bladder. The analysis of cells from the cervix is a minimally invasive procedure called a cervical or Papanicolaou smear (Pap smear). This involves the insertion of a speculum into the vagina to allow the clinician to directly view the cervix. The cervix is then gently scraped to retrieve cervical cells which are smeared directly onto glass slides at the bedside and submitted to a laboratory for examination. The material from the cervical scrape can also be directly tested for wart virus (Human Papilloma Virus), the major risk factor for the development of cervical cancer.

Aspiration Cytology

This is the analysis of cells from within a mass or organ. This involves a more invasive sampling procedure called Fine Needle Aspiration (FNA). A needle is inserted into the area of the body being examined, sometimes with the use of imaging (e.g. ultrasound or CT scan) to ensure that the suspicious area is being sampled. This procedure may be performed after injection of local anaesthetic to numb the skin, or even under light sedation if involving a deep organ or tissue. The cells retrieved are expressed onto a slide and prepared in a similar way to the cervical smear. If fluid is aspirated (e.g. within from a thyroid cyst), it may first be spun by a centrifuge so that the cell-containing sediment collects at the bottom of the test tube, allowing the best material to be sampled for examination.

Examining Cytology Material

The most common samples in cytology are exfoliative, including cervical smears (Pap smears), urine and sputum. These are usually screened by trained cytotechnicians or, in some laboratories, Computerised automated systems, to look for any suspicious cells. Suspicious samples are forwarded to a pathologist for further microscopic examination and final diagnosis. Aspirated material is usually viewed by a pathologist directly.

Special stains are performed to highlight the cells and background material on the slide, in a similar way to histopathology sections.

Shamim Akhter Ansari

B.Sc ( Medical Laboratory Technologist )

Punjab Technical University, Punjab India,

Date :

Place :

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