Research Scientist R D

MANDEEPKAUR

MANDEEP KAUR, PhD

E-mail: **********@*****.*** Mississauga, ON, L4T 3R5

Phone: +1-647-***-**** Canada (Permanent Resident) PROFESSIONAL SUMMARY

Ambitious and dedicated Biology and Biochemistry research scientist with nine years R and D experience in research laboratory setting. Well-versed with dissection, fixation, section cutting and visualization of rat, fish, insects, microorganisms, cell lines, and animal tissues. Manuscripts and reports/ project writing skills.

WORK EXPERIENCE

CELL BIOLOGIST

CREM Co Labs, Canada Feb 2022 - present

Cell culturing for GLP and R&D, routinely passage and plate cell lines.

Aliquoting and preparation of tissue culture reagents. Maintaining the cell culture related equipment.

Creating cell banks and developing SOPs for cell culture.

Working on primary tissue cultures including animal and human cells.

Performing efficacy tests on viruses, mycobacteria, fungi, aerobic and anaerobic bacteria using standard methods of AOAC and ASTM.

Preparation and maintenance of frozen stocks of bacteria, viruses and cell cultures. EDUCATION

PhD (Life Sciences/ zoology)

Guru Nanak Dev University, India 2014-2020

Thesis: Biochemical, genotoxic and cytotoxic effects of Entomopathogenic Fungi on Spodoptera litura (Fabricius). 10+ Publications.

Isolated various fungi from different biological sources and their subsequent screening for determination of their insecticidal potential.

Selected fungi were Identified on morphological and molecular basis and performed their toxicity and environment safety studies.

Toxicity of selected strains were evaluated on insects (S. litura) and mammals (CHO cell lines, Wistar albino rat) by in vitro and in vivo studies by employing genotoxicity (comet assay or single cell gel electrophoresis, chromosomal aberrations, micronuclei assay), cytotoxicity (cell viabilities studies under light and fluorescent microscope, scanning electron microscopic studies) and biochemical parameters (enzymes assays).

Identified various metabolites in fungal extracts by UHPLC and LC-MS studies.

Extraction and partial purification of fungal metabolites by employing different organic solvents.

Checked genotoxicity of environment pollutants such as azo dyes, on rat via MANDEEPKAUR

chromosomal aberration assay, by utilizing rat bone marrow.

Checked environmental pollutants (Organic flame retardants) induced toxicity in various organs of the freshwater fish ‘Channa punctatus’.

Tested genoprotective potential of fungi on fish DNA (by employing comet assay). Bachelor of Education

Guru Nanak Dev University, India 2013-2014

Gained teaching and organizational skills.

Master of Science (Molecular Biology and Biochemistry) Guru Nanak Dev University, India 2009-2011

Thesis: Antioxidant and antiproliferative activity of Tinospora cordifolia plant extract on C6 glioma cell lines.

Prepared different plant extracts utilizing various solvents.

Determined antioxidant activity by DPPH and reducing power assay.

Determined antiproliferative activity on C6 glioma cell lines via MTT assay.

Extraction of compounds of varying order of polarities from plants by utilizing various organic and aqueous solvents.

Used ELISA plate reader, UV vis spectrophotometer and haemocytometer.

Awarded M.Sc. degree in first division.

Bachelor of Science (Medical)

Guru Nanak Dev University, India 2006-2009

Gained conceptual understanding and knowledge of Botany, Zoology and Chemistry as major subjects.

Awarded B.Sc. degree in first division.

Publications (Peer Reviewed) and presentations/ Workshops

Mandeep Kaur, Pooja Chadha, Sanehdeep Kaur, Amarjeet Kaur, Rajvir Kaur, Arun Kumar Yadav, Ramandeep Kaur, Schizophyllum commune induced genotoxic and cytotoxic effects in Spodoptera litura, Nature- Scientific reports, 2018, 8, 4693. Impact factor 4.9

Mandeep Kaur, Pooja Chadha, Sanehdeep Kaur, Amarjeet Kaur, Rajvir Kaur, Arun Kumar Yadav, Ramandeep Kaur, Evaluation of Genotoxic and Cytotoxic effects of ethyl acetate extract of Aspergillus flavus on Spodoptera litura, Journal of applied microbiology, 2019, 126, 881-893. Impact factor 4.1

Mandeep Kaur, Pooja Chadha, Sanehdeep Kaur, Amarjeet Kaur, Prince Sharma, Effect of Aspergillus flavus on Glutathione-S-Transferase activity of Spodoptera litura, Asian journal of microbiology and biotechnology, 2018, NAAS Rating 4.93

Mandeep Kaur, Pooja Chadha, Sanehdeep Kaur, Amarjeet Kaur, Rajvir Kaur Schizophyllum commune induced oxidative stress and immunosuppressive activity in Spodoptera litura. BMC Microbiology, 2020. Impact factor 4.5 MANDEEPKAUR

Mandeep Kaur, Pooja Chadha, Sanehdeep Kaur, Amarjeet Kaur, Prince Sharma. Effect of Aspergillus flavus on lipid peroxidation and Activity of Antioxidant Enzymes in Midgut Tissue of Spodoptera litura larvae, Archives of phytopathology and plant protection. 2020, Impact factor 0.7

Mandeep Kaur, Pooja Chadha, Sanehdeep Kaur, Amarjeet Kaur. Aspergillus flavus induced oxidative stress and immunosuppressive activity in Spodoptera litura as well as safety for mammals. BMC Microbiology, 2021. Impact factor 4.5

Rajvir Kaur, Jasleen Kaur, Mandeep Kaur, Vishali Kalotra, Pooja Chadha, Anupam Kaur, Amarjeet Kaur. An endphytic Penicillium oxalicum isolated from Citrus limon possesses antioxidant and genoprotective potential. Journal of applied microbiology, 2019, 126, 881-893. Impact factor 4.1

Rahul Datta, Amandeep kaur, Isha Saraf, Mandeep Kaur, Inder Pal Singh, Sanehdeep Kaur, Pooja Chadha. Assessment of genotoxic and biochemical effects of purified compounds of Alpinia galanga on a polyphagous lepidopteran pest. Phytoparasitica, 2020. Impact factor 1.8

Prince Sharma, Pooja Chadha, Mandeep Kaur. Organic flame retardant induced oxidative stress in various organs of freshwater fish Channa punctatus. Pollution Research, 2018, NAAS Rating 4.97

Mandeep Kaur, Pooja Chadha, Sanehdeep Kaur and Amarjeet Kaur. Cytotoxicity Analysis of Partial Purified Extract of Schizophyllum commune (Schizophyllaceae) against Spodoptera litura (Fabricius) (Lepidoptera: Noctuidae). Proceedings MDPI,2021

Mandeep Kaur, Pooja Chadha, Sanehdeep Kaur, Amarjeet Kaur. Entomopathogenic fungi as biocontrol agents. Under review; archives of microbiology.

Mandeep Kaur, Pooja Chadha, Sanehdeep Kaur, Amarjeet Kaur. In vivo Safety evaluation of an entomopathogenic endophytic fungus Schizophyllum commune using rat as a model. 2022. Under review; FEMS Microbiology letter.

Rajvir Kaur, Jasleen Kaur, Avinash Sharma, Mandeep Kaur, Vishali Kalotra, Pooja, Chadha, Anupam Kaur, Amarjeet Kaur. An endophytic Talaromyces verruculosus with alpha glucosidase inhibitory, antioxidative and genoprotective potential. 2022, Submitted.

9 presentations/ workshops attended.

Academic Honors, Awards and Fellowships

Best poster award in national seminar on “Recent trends in Plant and Environmental Sciences”, Guru Nanak Dev University, India.

Awarded merit certificate in B.Sc. and M.Sc.

Awarded full international travel grant by SERB and CSIR to present my PhD work in an international conference in New Brunswick.

MANDEEPKAUR

Skills/ Strengths

PhD in life sciences and M.Sc. in molecular Biology and Biochemistry.

Isolated genomic DNA by organic and inorganic methods, including quantification of DNA using spectrophotometric methods, restriction digestion etc.

Separated immune cells, TLC, DLC including lymphocyte culture and immunoassays, use of haemocytometer etc.

Electrophoresis: Agarose gel electrophoresis, SDS-PAGE.

Used spectroscopic techniques to estimate total and reducing sugars, absorption maxima of aromatic amino acids, protein estimation by Biuret, Bradford, Lowry and UV methods etc.

Genotoxicity testing by comet assay, cytotoxicity testing by using different fluorescent dyes under fluorescent microscope and by Scanning Electron Microscope (SEM).

Practical handling of rat and fish. Insect culturing (culturing of Spodoptera litura).

Isolation and maintenance of culture of microorganisms such as Isolation of endophytes

(fungi from plants), slide culture techniques for fungi, production and extraction of metabolites.

Well versed with spectroscopic and physical research techniques. Report writing.

Aware about the safety rules while using chemicals. Independent as well as team worker who respect tight deadlines.

Possess broad skills in Molecular and Cellular Biology, Microbiology and Biochemistry, Entomology, Toxicology and Environment safety studies.

DNA isolation from different organisms like rat, fish, human, microorganisms.

Quantification and amplification (PCR) of DNA.

Biochemical studies (oxidative stress studies, enzymes assays). Immunological assays

(ELISA).

Microbiology techniques (microorganisms culturing, mass production and microbial testing in various samples.

Rearing of different animals such as insects (Spodoptera litura), fish, rat etc.

Environment studies like toxicity testing of environment pollutants on different organs of rat (DNA damaging studies by comet assay and chromosomal abbreviations) and fish

(check aquatic pollution).

Cell lines handling (culturing, subculturing, toxicity testing on cell lines). UHPLC and LC- MS studies.

Instruments/ Softwares handled

BOD Incubator, Light microscope, Fluorescent microscope, SEM (Coating, sample preparation), UV-vis spectrophotometer, Rotavapor, Laminar flow, Autoclave, Orbit shaker, LC-MS, HPLC.

MEGA7 (Evolutionary study, Phylogenetic tree construction), CASP LAB (Comet scoring), Stat Softwares (Multiple), Data presentation: MS office (PowerPoint, Word, Excel etc), Origin 70.

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