Research Associate Molecular Biology

Dhondup Namgyal

Phone: +1-323-***-****

Email: ************@*****.***/******@***.***

PROFESSIONAL SUMMARY:

Self-motivated Neuroscience researcher with expertise in handling animal models (rat and mice), molecular biology techniques (DNA and RNA isolation, real-time PCR, gene expression quantification, epigenetic study), biochemical techniques (protein quantification, mass spectrometry, and enzyme assays), cell biology techniques (iPSC, ESC, Motor neuron and brain organoids culture), immunology techniques (immunohistochemistry, immunocytochemistry and western blotting), and microscopic techniques (electron microscope, fluorescent microscope, and confocal microscope).

PROFESSIONAL EXPERIENCE

July 2024- Current

Postdoctoral Research Associate /University of Southern California, CCMB, US

- Role of MSC in Meningeal Lymphatic Functions.

- Investigate effects of glymphatic perturbations by AQP4 on craniosynostosis and regeneration craniosynostosis mice.

- Generation of iPSC derived Synovial Organoids to study arthritis and pain. MARCH 2022-AUGUST 2023

Postdoctoral Researcher/Wonkwang university, South Korea

-Development of motor neurons and the c2c12 co-culture system to model neuromuscular related neurodegenerative disease.

-Development of neuromuscular organoids from ipsc (als patient) and esc to elucidate the cellular and molecular pathways involved in neurodegenerative disease.

-Developing the lc3/gabarap-selective sensors for selective autophagy in different models of neurodegenerative diseases.

-Setting up and conducting behavior tests, stereotaxic surgery, handling different neurodegenerative disease mice models (abcd4, tdp43, 5xfad and dscam-cre), immunohistochemistry, and immunocytochemistry.

SEPTEMBER 2017-JULY 2018

Visiting Faculty /Amity university, Noida, India

-Taught following courses to b.sc. (h) neuroscience student. 1. Olfaction and taste (45 sessions)

2. Nerve physiology (30 sessions)

3. Fundamentals of neurophysiology (4 sessions)

4. Social neuroscience (4 sessions)

-55 minutes per session.

EDUCATION

2015-2021

Ph.D. in Neuroscience/Amity University University Noida 80% 2012-2014

M.Sc. Biotechnology/Bangalore University 64%

2009-2012

B.Sc. Biotechnology /Christ University, Bangalore 66% 2007-2009

C.B.S.E 11th and 12th Grade /TCV School, Dharamshala 72% RESEARCH PUBLICATIONS:

● Namgyal, D. Lim, CS. (in preparation). Advantages of 3D Brain Organoids: Bridging the Gap Between 2D Cultures and In Vivo Models.

● Namgyal, D. Lim, CS. (in preparation). Small Molecules in Organoid Culture: Enhancing Development and Applications

● Namgyal, D., Ali, S., Hussain, M. D., Kazi, M., Ahmad, A., & Sarwat, M. (2021). Curcumin ameliorates the Cd-induced anxiety-like behavior in mice by regulating oxidative stress and neuro-inflammatory proteins in the prefrontal cortex region of the brain. Antioxidants, 10(11), 1710.

● Namgyal, D., Chandan, K., Ali, S., Ahmad, A., Hashim, M. J., & Sarwat, M. (2021). Aberrant lighting causes anxiety-like behavior in mice but curcumin ameliorates the symptoms. Animals, 11(9), 2590.

● Namgyal, D., Ali, S., Mehta, R., and Sarwat, M., 2020. The neuroprotective effect of curcumin against Cd-induced neurotoxicity and hippocampal neurogenesis promotion through a CREB-BDNF signaling pathway. Toxicology, 442, p.152542.

● Namgyal, D., Chandan, K., Sultan, A., Aftab, M., Ali, S., Mehta, R., El-Serehy, H.A., Al- Misned, F.A. and Sarwat, M., 2020. Dim light at night induced neurodegeneration and the ameliorative effect of curcumin. Cells, 9(9), p.2093.

● Namgyal, D., Chandan, K., Ali, S., Mehta, R. and Sarwat, M., 2020. Curcumin improves the behavior and memory in mice by modulating the core circadian genes and their associated micro-RNAs. Journal of Pharmacology and Pharmacotherapeutics, 11(2), p.44.

● Namgyal, D., Chandra, A., Reddy, G. and Kumar, K., 2017. Biosorption of heavy metal using bacteria strain and its optimization. Indian journal of pharmacology, 43(3), p.246. BOOK CHAPTER PUBLICATIONS:

● Ghosh, S., Aziz, A., Namgyal, D., & Sinha, J. K. 2022. Electromyography. In Encyclopedia of Sexual Psychology and Behavior (pp. 1-4). Cham: Springer International Publishing.

● Namgyal, D., Sarwat, M., 2020. Saffron as a neuroprotective agent, in: Sarwat, M., Sumaiya, S. (Eds.), Saffron: The Age-Old Panacea in New Light. Elsevier. pp 93-102. CONFERENCE PRESENTATION

● Dhondup, Namgyal (2014). Studies on Lead biosorption by bacterial isolates and its optimization. Presented at the “Inter Deanery Conference” at Oxford College, and Bangalore University, Bangalore.

● Dhondup Namgyal (2014). Oral presentation and poster presentation of “Recent Advances in Biotechnology” at 6th National science conference” held on 27th-28th of March 2014 at the Oxford College of Science, HSR Layout, Bangalore.

● Dhondup Namgyal (2019). The neuroprotective effect of curcumin against Cd-induced neurotoxicity and hippocampal neurogenesis promotion through a CREB-BDNF signaling pathway. Presented at the “Exploring Mind, Brain and Behaviour” conference at Amity University, Noida.

● Dhondup Namgyal (2019). Neuroprotective effects of curcumin are associated with modulation of major circadian rhythm genes and their associated miRNAs (microRNA21a-5p and microRNA34a-5p) during dim light at night (dLAN) induced neurotoxicity in the Swiss Albino mice model. Poster presentation at the “Interdisciplinary Science Conference on Big Data and Computational Biology” held between 21st and 22nd October 2019 at Jamia Millia University, Delhi.

References

● Dr. Maryam Sarwat (Professor). Amity Institute of Pharmacy, Amity University Noida. Email ID: *******@*****.*** Ph. No: 999*******

● Dr. Jitendra Kumar Sinha, Ph.D. Chief Scientific Officer, GloNeuro Academy, Noida. Email ID: *******.**@*****.*** Ph. No: 891-***-****

● Dr. Arundhati Ray. Assistant Professor, School of Medicine, University of Nottingham UK. Email ID: *********.***@**********.**.**

● Chae-Seok Lim, Ph. D. Associate Professor. Department of Pharmacology, Wonkwang University School of Medicine, Jeonbuk 54538, Korea. Ph. No. 82-63-850-****. E-mail: ********@***.**.**

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