Protein Purification Drug Development

TERESE PEPPLER

**** ***** **, ******* ***** IL 60516 P HO NE: 630-***-****

E-MAIL: *************@*****.***

EXPERIENCE

ABBOTT LABORATORIES (Temporary contract by US Tech Solutions)

**/** ** 06/23

(Drug development)

Scientist Protein purification by various harvest, lysis, chromatography, filtration, and formulation techniques.

•Extensive training in GLP, GDP, and safety

•HPLC purity and data interpretation

INVENRA, Madison, WI 11/21 to 02/2022

(Drug developmentt)

Scientist II Protein scientist responsible for purification of bispecific antibodies, through various chromatography, filtration, and formulation techniques.

•5S activities and procurement of laboratory equipment

•Interpreted PubMed Literatures and shared with the team

Promega Corporation, Fitchburg, WI 02/20015 to 02/2020

(Drug development / industry support)

Protein Purification Scientist IV Protein scientist responsible for purification of primarily enzymes, or antibodies, through various chromatography, filtration, and formulation techniques.

•Followed or authored SOPs and BPRs pertinent to GLP/GMP protein manufacture and analysis according to GDP standards.

•Performed various protein and DNA assays to ensure that products met or retained specifications. Analyzed and compiled results according to GDP standards.

•Developed process improvements to replace chromatography resins and TFF equipment. Ensured changes produced pilot or scale-up batches resulting in products that met the specifications and client needs.

Catalent Pharma Solutions, Madison WI 2009 to 02/2015

(Global drug development, delivery and supply partner for drugs, biologics and health products.)

Protein Purification Scientist and Associate Scientist

Protein scientist responsible for development of novel antibodies, fragments, and conjugates through various chromatography, filtration, and formulation techniques.

•Independently developed and optimized downstream processes for the purification of biopharmaceuticals, scale up of productions, and authored tech transfers for GMP productions.

•Successfully provided the clients with written development and scale up reports, composed batch production records, and perform tox lot viral validation studies. All reports were accurate the first time.

•Co-led the site safety committee, led the ergonomics program, and contributed as a continuous improvement leader as a Six Sigma Green Belt.

Life Technologies, Invitrogen, Madison WI 2006 to 2009

(Global provider of Biotechnology with sites in over 100 countries.)

Production Associate III, Manufacturing

Protein scientist responsible for production of catalogue proteins through various cell lysis techniques, protein purification strategies, and product validations.

•Consistently produced protein product that passed purity, concentration, and specific activity specifications with batch documentation that meets ISO traceability standards.

•Successfully contributed to team efforts to reduce cycle time, increase productivity and availability.

•Developed methodologies to reduce process deviations and reportable findings in batch documentation.

Associate Scientist III, Protein Contract Services

Provided clients with project results or finished product designed to meet their custom needs. Strategically developed methods of protein production by determining ideal expression conditions and chromatography applications.

•Delivered > $90,000 revenue in one week by independently carrying out two mid-scale, 5 step protein productions in parallel.

•Leveraged multiple projects concurrently with the ability to quickly redirect focus in response to priority.

•Collaborated between team and Quality Assurance to develop and carry out a quality plan that would bring our operations and documentation practices within the ISO standards. Contributions include the authorship of SOPs and Forms that provided production traceability.

•Provided relief to stressed departments by acting as a temporary fermentation scientist and performing tasks in molecular biology that would expedite our downstream processing.

Argonne National Laboratory, Argonne, IL 2004 to 2006

(U.S. Department of Energy's Laboratory for Science and Engineering research.)

Contracted by On Assignment Lab Support

Research Scientist, Genomes to Life Project

Conducted experiments in high throughput cloning for protein expression and purification.

•Investigated and refined high-throughput strategies for DNA mutagenesis, cloning, protein expression, and purification.

•Wrote and exercised robot protocols for 96-well processing of DNA and protein.

•Utilized web-server tools to design oligos, generate sequences, and obtain peptide informatics.

•Developed methods for cytochrome expression, purification and detection via western blot.

•Generated databases archiving various protocols, experimental conditions, sequences and results to be used in publications. Inventoried clonal stocks for distribution to collaborators.

•Contributed to several publications by providing extensive data sets, methods, clones and purified proteins or cytochromes.

EDUCATION

Bachelors in Science- Biochemistry, University of Illinois Chicago

Volunteer Lab Assistant: Department of Biochemistry

Performed experimental tasks in support of molecular biology and protein studies.

•Demonstrated ability to follow instruction and work independently to provide quality results.

•Assisted studies by providing and analyzing PCR fragments, expressing and purifying protein.

SKI LLS/ AUTOMATED SYSTEMS

AKTA Explorer, Purifier, Prime, Avant, Process Skid,

Beckman Coulter FX

Biomek 2000

Mutagenesis (PCR Thermocycler)

Sartorius TFF + SciLog TFF Fluostar

DNA (Fragment or Plasmid)

SKI LLS/ QC ASSAYS

P32

SDS PAGE and Western Blot

Cytochrome detection and X-Ray

Glo Max

Colorimetric

Enzyme Activity Assay

DNA Binding

UV

Kinetic

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