R D Scientist Ii
Resume:
Summary:
I am a highly motivated scientist with over 8 years of academic research and nearly 2 years of industry R&D experience specializing in Biochemistry, Enzyme Assays, Protein Purification, and Analytical Instrumentation. Passionate about advancing early-stage technologies in synthetic biology, enzyme engineering for healthcare, and environmental sustainability, with expertise spanning basic to applied research. Thrives in collaborative, high-performance settings, dedicated to impactful scientific innovation through publications, international presentations, and leadership in mentoring. Professional Appointments:
Scientist II (09/2022 - current): Rubi laboratories, Alameda, California, USA
• Managing projects and roadmaps, DOE, and reporting to the CTO while supervising two RAs.
• Led projects on enzyme discovery, characterization, stabilization chemistry, and the engineering of multi-enzyme pathways for the synthesis of CO2-derived biomaterials.
• Mitigating enzyme inhibition in multi-enzyme pathways, specifically addressing issues related to inorganic phosphate and intermediate formation.
• Spearheaded in-house enzyme purification initiatives, including the isolation of challenging heterodimer membrane-bound proteins. Directed enzyme engineering projects focused on inhibition mitigation, enhanced stability, and increased total turnover number.
• Collaborated extensively with cross-functional teams and led projects in partnership with various contract research organizations to drive innovation and support company growth. Postdoctoral Fellow (10/2021- 09/2022) Department of Biochemistry, University of Utah, Utah, USA
• Conducted in-depth research on the structural and functional characterization of receptors, transporters, and ion channels associated with kidney diseases.
• Investigated molecular structures and pharmacological profiles of cation-chloride cotransporters
(CCCs), emphasizing protein-protein and protein-ligand interactions using advanced biophysical techniques (SPR, ITC, DSF).
• Engaged in sophisticated protein engineering, complex cloning strategies, and the establishment of stable cell lines. Achieved high-yield protein expression including membrane protein, and ion channels utilizing bacterial, insect, and mammalian cell systems.
• Performed Cryo-Electron Microscopy (Cryo-EM) sample preparation and executed cell-based functional assays.
Education:
Ph.D. in Biological Sciences (07/2015 – 09/2021)
Indian Institute of Science Education and Research (IISER) Kolkata, India Thesis: "Molecular insights into glucose tolerance of GH1 β-glucosidase and application in cellulase cocktail"
M.S. in Biological Sciences (07/2013 - 06/2015)
Indian Institute of Science Education and Research (IISER) Kolkata, India Bachelor of Science (07/2009 - 05/2013)
Institute of Agricultural Sciences, Banaras Hindu University, Varanasi, India Sushant Kumar Sinha (Ph.D.)
Scientist II
314 Caliente drive
San Leandro, CA 94502, US
****************@*****.***
https://www.linkedin.com/in/sushantks/
Google scholar
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Experimental Skills:
Molecular Biology Techniques: Cloning, gene design, vector construction, Gibson assembly, site- directed mutagenesis.
Protein Expression and Purification: Expression in E. coli, yeast, insect cells, mammalian cells, and cell-free systems. Purification using affinity chromatography, ion exchange chromatography, and size exclusion chromatography.
Automated Purification Systems: ÄKTA and Bio-Rad FPLC. Electrophoresis and Protein Analysis: Agarose, SDS-PAGE, Native-PAGE, silver staining, ELISA, and Western blotting.
Protein Quantification and Characterization: UV-Vis spectroscopy, Bradford and BCA assays, high- throughput enzyme assays, cellular assays, enzyme kinetics and MOA studies using plate readers, HPLC
(Waters, Agilent).
Spectroscopic Techniques: UV-Vis, fluorescence, circular dichroism, differential scanning fluorimetry
(DSF), Nano DSF, dynamic light scattering (DLS).
Biophysical Analysis: Isothermal Titration Calorimetry (ITC) and surface Plasmon Resonance (SPR) using Biacore and Nicoya OpenSPR systems.
Soft Skills:
Protein Visualization Tools: PyMOL, UCSF Chimera, BIOVIA Discovery Studio, Visual Molecular Dynamics.
Protein-Ligand Docking: AutoDock 4.2.6, AutoDock Vina. DNA and Protein Analysis Tools: Multiple sequence alignment, oligomer synthesis, homology modeling, AlfaFold.
Microsoft Office Suite: Excel, Word, PowerPoint, Project. Data Analysis and Graphing Software: GraphPad Prism, Origin. Electronic Lab Notebooks: Benchling.
Publications (chronological order):
1. Sinha, SK; Datta, S# Applied Microbiology and Biotechnology, 2016, 100(19), 8399–8409 (IF: 5.2) DOI: 10.1007/s00253-016-7601-x, Cited: 58
2. Sinha, SK; Goswami, S; Das, S; Datta, S# Applied Microbiology and Biotechnology, 2017, 101(4), 1455–1463 (IF: 5.2) DOI: 10.1007/s00253-016-7904-y, Cited: 29 3. Mukherjee, I*; Sinha, SK*; Datta, S#; De, P# Biomacromolecules, 2018, 19(6):2286-2293 (IF: 6.2) DOI: 10.1021/acs.biomac.8b00258, Cited: 37
4. Konar, S; Sinha, SK; Datta, S#; Ghorai, PK# ACS Omega, 2019, 4, 11189−11196 (IF: 4.1) DOI: 10.1021/acsomega.9b00509, Cited: 25
5. Sinha, SK; Reddy, K. Prakash; Datta, S# Carbohydrate Research, 2019, 486:107835 (IF: 3.1) DOI: 10.1016/j.carres.2019.107835, Cited: 18
6. Sinha, SK; Das, S; Konar, S; Ghorai, P.K#; Das, R#; Datta, S# International Journal of Biological Macromolecules, 2020, 156, 621–632 (IF: 8.2) DOI:10.1016/j.ijbiomac.2020.04.036, Cited: 20 7. Konar, S; Sinha, SK; Datta, S#; Ghorai, P.K# Journal of Molecular Liquids, 2020, 306, 112879 (IF: 6.1) DOI: 10.1016/j.molliq.2020.112879, Cited: 20
8. Konar, S; Sinha, SK; Datta, S#; Ghorai, P.K# Journal of Biomolecular Structure & Dynamics, 2020, Jul 27:1-1. (IF: 4.4) DOI: 10.1080/07391102.2020.1796789 Cited: 5 9. Sinha, SK#; Datta, M; Datta, S#; RSC Green Chemistry. 2021. (IF: 9.8) DOI: https://doi.org/10.1039/D1GC01357B Cited: 17 (corresponding author). 10. Sahu S, Ghosh S, Sinha, SK, Datta S, Sengupta N. Biochemistry. 2023 Dec 5;62(23):3440-3452. DOI: 10.1021/acs.biochem.3c00387. (IF: 2.9)
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Patent applications (filed):
1. Glucose Tolerant GH1 β-glucosidase from Thermomicrobium roseum and compositions thereof” Datta, S.; Sinha, S.K.; Datta, M.; (Application # 202*********, 2021) 2. Cloning of multifunctional secretory GH5 Endoglucanase” Datta, S.; Sinha, S.K.; Ghosh, D.; Mohan, S.V. (Application # 202*********, 2021)
Book Chapter:
Sinha, S. K., Datta, S# “Recent advancements in the engineering of β-Glucosidases for biomass degradation in Advances in Biofuels Production, Nova Science, New York, ISBN: 978-1-53614-671-4 Teaching and mentoring experience:
1. Jan- Apr 2019: Teaching assistant for LS4207 (Biochemistry & Structural Biology Laboratory), a 4th- year laboratory course.
2. Mentored 2 RAs at Rubi, 4 M.S. students in their thesis work, and 5 summer interns at IISER Kolkata. Selected conferences and awards:
1. EMBO India Bioscience Workshop on Oral Communication in Science, 12-29 Jan 2021 2. Poster presented at Gordon Research Seminar on Glycan Conversions, 20-21 July 2019, Andover, US 3. Poster presented at the GRC on Glycan Conversions, 21-26 July 2019, Andover, US 4. SERB travel award for GRS (20-21 July 2019) and GRC (22-26 July 2019), Andover, US 5. CSIR Travel grant for GRS (July 20-21, 2019) and GRC (22-26 July 2019), Andover, US 6. 1st position in Oral presentation (Young Scientist), BPI India -2018, 16 - 18 Dec 2018, IIT Delhi 7. Workshop on CRISPR/cas9 based genome editing, 13-17 March 2018, IGIB, Delhi, India 8. Best poster award, “Bioprocess India-2017”, 09-12 December 2017, IIT Guwahati, India 9. Science Communication Workshop, Welcome Trust/DBT India, 7-8 Sept. 2017, Delhi, India 10. Workshop on computational modeling in structural biology, 2-3 August 2017, MBU, IISc, India 11. ACS Omega Best poster award in “Smart Materials: Methods and Applications”, 20-22 April 2017, CAFM, IISER Kolkata, India
12. Best poster award in “Chemistry Interfacing with Biology and Physics”, 27-28 Jan 2017, IISER Kolkata, India
13. Best poster award in “Frontiers in Modern Biology”, 5-6 December 2015, IISER Kolkata, India. 14. Secured national CSIR fellowship for 5 years, December 2013, (All India Rank 32) 15. IIT-Joint Admission Test-2013, All India Rank 06. 16. ICAR-JRF, 2013, All India Rank 09, UoH, Int. Ph.D. (Biotechnology)-2013, All India Rank 01. Professional Affiliations:
1. Member of Americal Chemical Society (04/2024 - current) 2. Member of Biochemical Society (04/2024 - current) 3. Secretory, IISER-K Alumni Association (02/2024 - current) Social/cultural/outreach activities:
1. Member of Task Force committee, IISER K campus against COVID-19 (04/2019-07/2021), India 2. Founding member of the SMC (08/2014 – 10/2016), IISER K, India 3. Student representative of Integrated Ph.D. for UGAC (2014-2015), IISER K, India 4. Core organizing committee member of Inter-IISER Sports Meet-2015, Kolkata, India 5. Served 2 years in NSS (2010-2012), an Indian government public service program
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