Molecular Biology Medical Lab

Hannah Fan. M.D. M.Sc. Ph.D.

Greenville, Rhode Island, USA. ***************@*****.***. Phone: 508-***-****

SUMMARY OF QUALIFICATIONS

Clinical Medicine Fields Scientist with more than 25 years experiences in the fields of Hematology, Blood banking,molecular biology, immunology, microbiology and genetics at universities including Johns Hopkins University.

Teaching and research in the immunology and microbiology

Completed ten research projects in USA. and in China

Techniques qualification involved:

In medical lab :the routine test: blood, urine, feces

In immunology: ELISA, RIA, Eli Spot, Flow cytometry, immunohistochemistry staining, vitro assays, Proliferation, immunoprecipitation. PBMC Isolation, T cells and T cell cloning Immunofluorescence staining,,

In medical microbiology:, identification and culture of bacteria, virus, fungus,

In Pathology: Prepared microscopic slides in the research which includes tissue fixation, trimming, dehydration, infiltration, embedding, microtomy, mounting and sectioning of paraffin tissue blocks, Performed histochemical and immunohistochemical stains on the mice thyroid tissues using automated staining platforms (i.e. Ventana).

Prepared and maintained all necessary instruments and reagents, including stains, alcohols, and paraffins according to proper specifications.

In molecular biology: Purification of proteins, Small animal manipulation-mice, rat, guinea pig, Cell and tissue culture-CHO, GH3.Hela. HEK-293. Siha, transfect ion, transformation, chemiluminescence assay, Prepare monoclonal antibody and polyclonal Antibody, Electrophoresis-horizontal and vertical agrose, acrylamide, 2-D gel and DGGE {denaturing gradient gel electrophoresis} Chromatography, plasmid cloning

DNA and RNA extraction from blood, tissue, squashed cell and tissue-culture

PCR, RT-PCR, qPCR and sequencing, Southern Blot, Northern Blot, Western Blot

Agilent Triple Quadrupole and Quadrupole Time-of-Flight (Q-TOF) mass spectrometers

Use confocal laser scanning microscope, electronic microscope, et al

Hematology: Specimen collection, evaluation, and processing • Blood smear preparation, evaluation, and differential • Complete blood count • Miscellaneous tests Routine coagulation tests • Blood banking: ABO and Rh typing • Antibody detection and identification • Compatibility testing • Direct antiglobulin test • Transfusion reaction testing • Processing and administration of blood products. Instrument preventive maintenance and troubleshooting • Quality control / assurance • Laboratory safety

In computer science: Computer software such as Microsoft Office Word/Excel/PowerPoint, etc.). Statistical analysis, data managements (Freezer works, LDMS, STARLIMS, GlobelTrace. Systems .et al)

PROFESSIONAL HISTORY

CVS, 2023-- now

Medical Lab. Scientist, 2022

Brigham and Women Hospital. Boston . MA. USA

Medical research and data management (Freezerworks,

LDMS, STARLIMS, GlobelTrace et al)

Scientist Shanxi Medical University, Taiyuan China . 2001–.2021.

Vice Deputy Director of MMI,Tutored and advised postgraduate students graduate thesis for theirs Master degree of Science in Department of Medical Immunology and Microbiology (MMI),

Professor in Shanxi Medical University .Taught undergraduate and postgraduate students in the field of medical Immunology and Microbiology

CEO and president of SXSI from 2014---2023.

Postdoctoral Fellowship & Visiting scholar, 1998–2001,

The Johns Hopkins University, Baltimore MD USA

Conducted research on molecular biology and Immunology at Johns Hopkins University, Department of Endocrinology and Metabolism

Department of Molecular Immunology and Microbiology,

Performed research work on autoimmune disease-thyroiditis

Medical Clinic Experience: 1979—1992, Taiyuan China. Treatment patients in Surgery, Internal medicine Obstetrics and Gynecology .Pediatrics, hematology Dermatology, Otolaryngology, Stomatology Oral science, Ophthalmology and emergency medicine and medical lab experiment.

Peer Reviewed Publication—25 Papers

EDUCATION

PhD. Shanxi Medical University, Majored internal medicine .PRC

Postdoctoral fellowship: Johns Hopkins University, postdoctoral research fellowship in medicine- endocrine and metabolic, USA

Certification of Beijing University: Advanced Biochemistry and Molecular Biology Laboratory Technique, Peking University, 1994, PRC

(Chromatography (HPLC, FPLC, Affinity chromatography, etc.)

M. Sc. Med degree, Shanxi Medical University PRC

M.D, Majored clinical medicine in Shanxi Medical University PRC

Certifications: Pharmacy tech. (effective from June 2022 to December 31, 2026), CITIPROGRAM issued by USA and others issued by P.R. China

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