Protein Biochemist R&D Scientist Assay/Method Development Sci
Resume:
Sagar M. Patel, Ph.D.
Lincoln, Nebraska
*********@*****.***
https://www.linkedin.com/in/sagar-m-patel-phd2020
Publications: https://scholar.google.com/citations?user=pHLSgtIAAAAJ&hl=en
Summary
■ Flexible and intrepid researcher scientist with expertise in bioanalytical molecular biology techniques, as evidenced by mentoring 6 professionals in method/assay development and scientific data analysis
■ Strong and effective communication of scientific data with an ability to present data for biophysical protein characterizations and enzyme activity experiments resulting in 2 research poster and slide presentations at ACS 2016 regional and 2018 national meetings
■ Innovative problem-solver in the field of in vitro assays with wide ranging versatility in disciplines of protein biochemistry, enzymology, reaction kinetics, biocatalysis, analytical chemistry, and structural biology research leading to the publication of 7 peer-reviewed scholarly documents on proline metabolism and cellular bioenergetics
Work Experience
R&D, problem solving, and collaboration with a team for assay development
Gained as Post-Doctoral Research Associate at University of Nebraska–Lincoln, 2020-present
■ Strongly driven with critical thinking and data analysis from experiments of plasmid DNA construction, bacterial transformation of genes, protein engineering efforts for overexpression then protein purification, biophysical characterization of kinetics and structure of enzymes, and mammalian cell culturing, all resulting in fulfillment of 2 NIH grants totaling $3.6 M
■ Troubleshooting ability to perform optimization of siRNA reverse transfection strategies of MCF-10A cells, resulting in selection of siRNA oligonucleotide candidates against proline metabolic genes combined with lipofectamine conditions that yield 72-hr post treatment siRNA gene knockdown of more than 50% shown on Western blots along with validation of severe hypoxia studies of cell cultures
■ Experience guiding and training junior members in column-based affinity chromatography protein purification and enzyme activity assays and process development expertise, using lab notebook protocols that led to production of 3 SOPs documentation
Technical literacy, project organizational skills, and mentorship experience
Gained as Ph.D. Graduate Student TA at University of Nebraska-Lincoln, 2016-2020
■ Interpersonal skills developed with 4 semesters’ experience, identifying opportunities to
Sagar M. Patel, Ph.D.
foster growth of 26-30 students and contributing to curriculum expansion in enzymology
■ Ability to present scientific data and concepts to others in engaging demonstrations of best practices for results, appropriate and safe cleanup of lab spaces, as well as provision of weekly assessments via graded pre-lab quizzes and research reports, as evidenced by completion of 14 weeks-worth of lab reports and capstone group oral presentations per semester
Project management skills with product and market knowledge
Gained as Hollister Incorporated-KCOM Clinical Research Fellow at A.T. Still University of Health Sciences-KCOM, 2010-2011
■ Time management skills and ability to collaborate with key multi-disciplinary teams of stakeholders for scientific and clinical project data, as evidenced by balancing 2 research projects: (1) completing proprietary corporate in vitro wet lab antimicrobial testing studies & (2) coordinating clinical research study involving MRI of ostomy/fecal continence care medical device fit in human subjects to guide manufacturing design
■ Strong work ethic and initiative with expertise in formulation of subject recruitment brochure and dealing with KCOM institutional review board for clinical research study, resulting in subject recruitment work in medical offices of 3 physician-investigators
Techniques, Software, & Technology
Bioconjugation and flavin removal assays to identify covalent adduct
Reverse Transfection of siRNA oligonucleotides
Mammalian cell culturing & N2 cryostocks preservation
E.coli plasmid subcloning via chemical/electroporation transformation
Colony PCR & site-directed mutagenesis
Genetic design of expression constructs
Recombinant protein expression & induction
Protein purification by column chromatography via IMAC, IEC, and SEC
SDS-PAGE & bulk wet transfer electro-Western blot
UV/Vis spectrophotometry for steady-state & stopped-flow enzyme kinetics
DSF measuring protein thermostability
CD spectroscopy of proteins
AUC-SV or -SE studies
TLC of reducing sugars
Propensity for learning advanced techniques: NMR, mass spectrometry, ELISA, qRT-PCR, and biocatalysis studies of RNA oligonucleotides
PyMOL
SigmaPlot (member of Gold Team user forum)
ChemDraw
SEDFIT
Knowledge of coding scripts in Python
Education
Doctor of Philosophy in Biochemistry 2014-2020
Thesis Dissertation: Characterization of Human Pyrroline-5-Carboxylate Reductase Enzymes Responsible for L-Proline Biosynthesis. University of Nebraska-Lincoln, Center for Biological Chemistry.
Affiliations & Hobbies
■ Member and/or volunteer in Cheeky Scientist Association, ACS, AAAS, and Sigma Xi
■ Performing aerobic fitness and anaerobic weight resistance exercises & solving puzzles and challenges from cognitive games on Lumosity.com
■ Reading sci-fi/fantasy adventure novels & viewing English-subtitled Japanese anime series
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