Molecular Biology Quality Control

I am a very motivated and organized professional with extensive experience in biomedical research. I am skilled in cell culture, molecular biology, and biochemistry techniques. Excellent in research planning, team leadership, and interpersonal skills. I can adapt quickly while developing standard operating procedures and troubleshooting laboratory equipment. Flexible and take the initiative, and I can be relied upon to help achieve organizational targets and goals. RESEARCH ASSOCIATE — Atherosclerosis(Lipoprotein Receptor Bio. Lab), University of Ottawa Heart Institute Ottawa, ON, November 2017 - March 2024

LAB TECHNICIAN — Bio Geo Chemistry Research Group, University of Eastern Finland, Kuopio May 2017 - October 2017

Ikhuosho Charity

Asikhia

Phone +1-613-***-****

Email *********@*****.***

Address Ottawa, ON K2J6N3

• Good Laboratory Practice (GLP) • Grant Writing

• Molecular Biology • Cell Biology

• Protein Purification • DNA Extraction

• RNA Isolation • Protein Biochemistry

• Experimental Protocols • Phlebotomy

• Research Planning • SDS-PAGE and Western Blot

• Molecular Biology Techniques

• Site-Directed Mutagenesis

• Cell Biology Techniques

• SDS-PAGE and Western Blot

• DNA Extraction

• Protein Biochemistry

• HPLC - Protein Purification

• Research Planning

• Team Leadership

• Ability to Balance Multiple Priorities

• Quality Control

• Cell Biology

• Molecular Biology Techniques

PROFESSIONAL SUMMARY

SKILLS

EXPERIENCE

MEDICAL SUPPLY TEAM MEMBER (INTERN) — Suomen Punainen risti (Finnish Red Cross) Joensuu, Eastern, September 2016 - April 2017

BIOLOGY/CHEMISTRY LAB SUPERVISOR — Mother Patern College of Health Sciences, Monrovia, April 2011 - April 2013

LAB TECHNICIAN — St. Louis hospital

Kaduna, September 2009 - August 2010

DOCTOR OF PHILOSOPHY IN BIOCHEMISTRY

— University of Ottawa

Ottawa, CA, Mar 2024

POSTGRADUATE DIPLOMA IN BIOCHEMISTRY

— The Federal University of Technology

Akure, Nigeria, Sep 2015

SELECTED COURSES (ASSOCIATE IN SCIENCE (A.S.) IN MEDICAL LABORATORY TECHNOLOGY

— Mother Patern College of Health Sciences, Stella Maris Polytechnic Monrovia, Liberia, May 2012

Relevant Coursework

• Analyzed experimental results while maintaining compliance with specifications

• Collaborated with other scientists and fellow technicians on designing experiments and developing protocols

• Quality Control - Performed tests to ensure the accuracy of results.

• Ensure Efficient Medical Supply to the Team through detailed reports outlining progress toward the departmental goal

• Work with the Medical Team to Ensure a Smooth Flow of Operation

• Ensure Operational Standards are always Met

• Ensure the Correct Temperature is Maintained on all Items

• Ensured efficient operation of the Lab through regular audits of laboratory operations to identify areas for improvement

• Work with various Instructors to provide training to Students

• Ensure Operational Standards are always met

• Established quality assurance and quality control processes to ensure the accuracy of results

• Instructor for third-year Chemistry

• Instructor - Nursing (Introductory to Biochemistry for first-year Nursing student).

• Followed safety protocols and procedures to maintain a safe working environment

• CD4 counts

• Routine Chemistry Test

• Clinical Microbiology

• Lab Management.

EDUCATION

HIGHER NATIONAL DIPLOMA IN SCI LAB TECH. (CHEMISTRY/BIOCHEMISTRY OPTION)

— Benue Polytechnic, Ugbokolo

Benue State, Nigeria, Feb 2009

• Parasitology • Clinical chemistry

• Human anatomy and physiology • Hematology

• Clinical microbiology • Urinalysis

Finnish

Conversational

Finnish language school (October 2015 -

September 2016)

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• International Doctoral Scholarship 2019 - 2021

• CIHR Travel Award 2022 - Best abstract Ph.D. category. Canadian Lipid & Vascular Summit

• Faculty of Medicine (uOttawa) Travel Award 2022

• University of Ottawa Heart Institute Travel Award 2022 Asikhia, I.; Chouli, L.; Kosenko, T.; & Lagace T. Proteolysis by furin improves PCSK9's binding interactions with native and oxidized low-density lipoprotein dependent upon prodomain retention. (2024) – Manuscript in Preparation

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Asikhia, I.; Renon, A.; Kosenko, T.; & Lagace T. An acidic N-terminal region in PCSK9 regulates LDL receptor binding affinity and prodomain association. (2024) – Manuscript in Preparation

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Sarkar, S. K., Matyas, A., Asikhia, I., Hu, Z., Golder, M., Beehler, K., Kosenko, T., & Lagace, T. A. (2022). Pathogenic gain-of-function mutations in the prodomain and C-terminal domain of PCSK9 inhibit LDL binding. Frontiers in physiology, 13, 960272. https://doi.org/10.3389/fphys.2022.960272

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Sarkar, S. K., Foo, A. C. Y., Matyas, A., Asikhia, I., Kosenko, T., Goto, N. K., Vergara-Jaque, A., & Lagace, T. A. (2020). A transient amphipathic helix in the prodomain of PCSK9 facilitates binding to low-density lipoprotein particles. The Journal of biological chemistry, 295(8), 2285–2298. https://doi.org/10.1074/jbc.RA119.010221

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Salawu, S. O., Olukemi, B. E., Asikhia, I. C., & Akindahunsi, A. A. (2018). Mineral Elements Bio-Accessibility and Antioxidant Indices of Blanched Basella Rubra at Different Phases of in vitro Gastrointestinal Digestion. Preventive nutrition and food science, 23(1), 22–29. https://doi.org/10.3746/pnf.2018.23.1.22

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LANGUAGES

AWARDS

PUBLICATIONS

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