Molecular Biology Laboratory Technician

Anna Puzinovici

*** ******* **, *******, ** M*H *B5

647-***-**** ad5ybs@r.postjobfree.com

SKILLS AND ABILITIES

Rich research experience in molecular biology and genetics laboratories

Experienced in analyzing datasets

Skilled in various molecular biology techniques such as Next Generation Sequencing, iPSC cell culture, microscopy, RNA extraction, PCR, Sanger sequencing

Experience with assay validation, data analysis and SOP writing

Computer skills with Microsoft office ie. Excel, Word, PowerPoint

Time management skills

Great organization skills, problem solving skills and eager to learn

Conscientious, reliable, and very well organized

Excellent interpersonal, oral, and written skills

EDUCATION

2019 York University

Bachelor of Science (B.Sc.) in Biomedical Science

2022-2023 University of Toronto: Data Analytics Bootcamp

Topics covered: Python, SQL, NoSQL, Machine Learning, Data cleaning, Visualization with Pandas and Matplotlib,, APIs, Tableu, Deep learning and Big Data

2023-2024 Seneca College

Bioinformatics Certificate

JOB EXPERIENCE

2023 Credit Valley Hospital, Genetics Lab

Laboratory Technician

Quickly became adept at understanding clinical genetics services within the hospital

Performed microarray testing and BCR-AML testing on blood samples

Extracted blood, amniotic samples, cultured cells on the Promega RSC 48 and

the FFPE Qiagen Extraction kit

2019-2022 Public Health Ontario, Molecular Diagnostics

Molecular Laboratory Technician

Validated a Covid-19 real-time PCR SNP assay for the K417 target to distinguish between the beta (B.1.1351) and gamma (P.1) variants of concern using limit of detection studies, as well as wrote the SOP

Worked closely with principle investigators to analyze the experimental dataset

Trained and lead a team of laboratory technicians on how to run the assay as well as how to interpret the results, performed troubleshooting on problem cases

Performed next generation sequencing using the Illumina (MiSeq and Next Seq) and Nanopore platform

Operated on various automated RNA extraction platforms (MGI, Chemagic), performed manual DNA extractions on bacterial samples, and SDS-PAGE

Trained new employees on various RNA extraction platforms, automated liquid handlers, setting up PCR as well as data analysis, and next generation sequencing

2020 Sick Kids Hospital

Lab Technician

Unpacked and sorted a wide variety of incoming specimens

Confirmed the accuracy of all patient information on requisitions and specimen samples, resolved any problems or inaccuracies prior to testing, and perform required data entry

Prepare and sort specimens for technical analysis

Perform weekly and monthly maintenance of laboratory equipment

Performed RNA extraction of viral samples

2018 Public Health Ontario

Summer Student

Evaluated sensitivity of 3 different types of sample collection swabs for detection of Bordetella by real time-PCR

Prepared primer and probe stock, calculated primer and probe dilutions

Performed Sanger sequencing

Gained experience in data management, analysis and writing SOPs

Developed strong theoretical and practical knowledge of molecular laboratory techniques

2017 Public Health Ontario

Summer Student

Quickly became skilled to use state of art technology, MALDI-TOF for identification of bacteria

Gained experience in using bioinformatics software for clinical application and data analysis

Performed molecular biological techniques such as DNA and protein extractions, and real time-PCR

Developed strong analytical and problem solving skills

Practiced fundamental microbiological techniques such as: working aseptically, serial dilution of bacterial samples and streaking

2017 York University

Laboratory Assistant

Gained experience in techniques such as PCR, gel electrophoresis, and western blotting

Maintained mammalian cell cultures and used microscopy to analyze cells for confluency

Became familiar with pipetting

Gained knowledge and experience in disinfection of experiment equipment and laboratory work areas

VOLUNTEER EXPERIENCE

2018-2019 St. Joseph Health Care Centre

Volunteer research assistant

Conducted a verification of a new sample collection swab, E-swab to facilitate automation of the microbiology diagnostics

Performed PCR on microbiological samples

Performed serial dilutions of microbiological samples and tested using Vitek

Built solid microbiology laboratory skills eg. serial dilutions, streaking microbiological samples

Had a deep understanding of the operation of a clinical microbiological laboratory

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