Molecular Biology Research Associate

Luis E. Perez

***** ***** ******, *** *******, TX 78233

210-***-**** ad5xwr@r.postjobfree.com or ad5xwr@r.postjobfree.com Languages: Fluent in English and Spanish, and able read and write. MICROBIOLOGIST & MOLECULAR BIOLOGIST

I am a molecular biology research professional with extensive research and development experience demonstrating outstanding organization, writing, and research skills. I have a natural ability to work with any individual as a team player or independently to get short- and long-term job objectives in a timely manner. The essential leadership skills gained in my professional career have prepared me to deal with difficult situations at work and outside work. CORE COMPETENCIES

Laboratory Management Educator Molecular Biology

Research & Development Protocol Development Top Leadership Skills Supervisor Skills Data Analysis Safety Official

EDUCATION

Master of Science in Biology, The University of the Incarnate Word, San Antonio, TX 2003 Bachelor of Science in Microbiology, The Ohio State University, Columbus, OH 1997 PROFESSIONAL EXPERIENCE

Uber Driver Jan 2017 to Present

11919 Alamo Blanco, San Antonio, Texas 78233

As an Uber Driver, I transport individuals from point A to point B in a safe and timely manner all over the state of Texas. I have completed over 6,650 trips. My current star rating is 4.95 and zero accidents. My professionalism allows me to provide excellent customer services while driving. To serve the community the best I can is my main goal every time I drive. Senior Research Associate Oct 2023 Feb 2024.

Texas Biomedical Research Institute, Integrated Research and Technology Core and Programs, 8715 Military Dr. San Antonio, Tx 78237-5302 In my previous position, I supported multiple investigators with sample preparation, flow cytometry analysis, and data processing. Flow Cytometry is a powerful technique used by investigators of the Texas Biomedical Research Institute to identify surface and intracellular markers associated with infection in different animal models. I assisted with processes associated with advanced molecular biology testing, and microscopy services, provided by the Integrated Research and Technology Core laboratory. I attended lunch conferences, completed mandatory online training, developed and wrote new SOPs in addition to helping with the daily operations of the core laboratory.

Biologist/Microbiology/ Duty Safety Officer July 2010 to March 2023 USA Army, Brooke Army Medical Center, Department of Clinical Investigation, 3400 Cawley E. Chambers Ste A, FT Sam Houston, TX 78234

I was employed to serve as the microbiology and molecular biology scientist for the Department of Clinical Investigation (DCI), US Army. In this capacity, I provided support to Principal Investigators with ongoing or new approved research protocols in the fields of Molecular Biology, Immunology, Biochemistry, Cell Biology, Cancer, Microbiology, Microscopy, and other fields of training and expertise as required. I reviewed, evaluated, and developed experimental protocols in support of the Graduate Medical Education (GME) research mission. I cross- trained DCI personnel including civilians, enlisted soldiers, fellows, and contractors in the proper use of instruments and scientific processes as part of DCI missions. I reviewed literature pertaining to research topics of interest and attended scientific conferences to expand knowledge and skills in areas of expertise. Additionally, I was heavily involved in the daily operations of DCI laboratories and safety regulations. Soccer Coach/Trainer March 2003 to November 2023

GRYSA/Arsenal, 75 Maske Rd, Schertz, Texas 78154

The idea and desire to create a soccer program to teach kids how to play soccer was born when my first son turned five years of age. The program was designed to teach young kids responsibilities and teamwork through sports. I created a soccer curriculum with a focus on dribbling, passing, shooting, and for kids to fall in love with the game as short-term objectives. The long-term objectives of the program were to develop high performing teams to compete against competitive soccer clubs. Understanding the game, and tactics was crucial for the teams to win most games.

Adjunct Faculty Aug 2004 to May 2015

St. Philip’s college, 1801 Martin Luther King Drive, San Antonio TX, 78203 As an adjunct faculty, my major responsibility of this position was to prepare & present lectures to students enrolled in the Microbiology and Clinical Pathology course at St. Philip’s college. I demonstrated basic microbiology principles in the laboratory section as part of the course. Biomedical Systems Analyst V Oct 2009 to Jul 2010

Brooks City-Base, 2484 Guillingham Drive, B-175W

Modified laboratory-based methods to design, plan, and perform experiments on a daily basis using the Joint Biological Agent Identification and Diagnostic System (JBAIDS) to provide rapid positive identification of possible pathogens found in food, clinical, or environmental samples. Microbiology Dec 2007 to Nov 2009

Brooke Army Medical Center (BAMC)

Department of Clinical Investigation, 3400 Rawley E. Chambers Ste A, FT Sam Houston, TX 78234

Involved in the development of a new electrochemical micro-array (ElectraSense) research protocol to differentiate influenza A (H1N1) from seasonal influenza. Research Technical Developer Jun 2005 to Jul 2006

Epidemic Outbreak Surveillance / Dept. of the Air Force – Advance Diagnostic Laboratory 2460 Pepperrel St., Lackland, AFB, TX 78236

Responsible for researching, learning, and implementing several methods to isolate RNA from human blood samples for gene expression studies. I independently learned and developed a high throughput assembly line consisting of several robotic systems (Bio-Robot Universal System, and Affymetrix Array Station) to isolate RNA from paxgene blood samples and screen possible blood biomarkers associated with upper respiratory infections. Biotechnology Scientist Sep 2003 to Jun 2005

Conceptual MindWorks, Inc., 4318 Woodcock Dr. San Antonio, Texas 78228 Involved in extensive literature research on topics related to spore neutralization to evaluate the killing efficacy of different metal nano-particles against Anthrax spores. Research Assistant Feb 1998 to Sep 2003

UTHSCSA, 7703 Floyd Curl Drive, San Antonio, Texas 78229-3900; Supervisor: At the University of Texas Health Science Center, I had the responsibility of helping with the general administration of the lab. In addition, I was expected to prepare and present weekly progress reports to my supervisor and colleagues as well as review and present scientific articles related to my projects. Supervising, and training students, doctors, and post-doctoral fellows in scientific processes were also required of me. PUBLICATIONS

1. Jane Shen-Gunther, Yufeng Wang, Zhao Lai, Graham M Poage, Luis Perez, TH Huang. HPV Deep sequencing of HPV E6/E7 genes reveals loss of genotypic diversity and gain of clonal dominance in high-grade intraepithelial lesions of the cervix. BMC Genomics. 2017 Mar 14;18(1):231

2. Jane Shen-Gunther, Chiou-Miin Wang, Graham M Poage, Chun-Lin Lin, Luis Perez, Nancy A Banks, TH Huang. Molecular Pap Smear: HPV Genotype and DNA Methylation of ADCY8, CDH8, and ZNF582 as an Integrated Biomarker for High-grade Cervical Cytology. Clin Epigenetics 2016; 8(1): 96 3. Perez LE, Merrill GA, Delorenzo RA, Schoenfeld TW, Vats A, Moser MJ. Evaluation of the specificity and sensitivity of a potential rapid influenza screening system. Diagn Microbiol Infect Dis. 2013 Jan; 75(1):77-80. Doi: 10.1016/j.diagmicrobio.2012.09.005. Epub 2012 Oct 18.

4. Livezey J, Perez L, Merrill G. Analysis of group A streptococcus gene expression in humans with pharyngitis using a microarray. Journal of medical microbiology." J Med Microbiol. 2011 Dec; 60(Pt 12):1725-33.

5. T.M. Straight, G. A. Merrill, L.E. Perez, J. Livezey, B. Robinson, M. Lodes, D. Suciu, and B. Anderson. A novel electrochemical device to differentiate novel influenza A

(H1N1) from seasonal influenza. Influenza Other Respi Viruses. 2010 Mar; 4(2):73-9. 6. Perez LE, Chandrasekar B, Saldarriaga OA, Zhao W, Arteaga LT, Travis BL, Melby PC. Reduced nitric oxide synthase 2 (NOS2) promoter activity in the Syrian hamster renders the animal functionally deficient in NOS2 activity and unable to control an intracellular pathogen. The Journal of Immunology, 2006 May 1; 176(9): 5519-5528. 7. Sara F. Kerr, Louise Emmons, Peter C. Melby, Chang Liu, Luis E. Perez, Maria Villegas, and Robert Miranda. Leishmania amazonensis infections in Oryzomys acritus and Oryzomys nitidus from Bolivia. Am. J. Trop. Med. Hyg., 75(6), 2006, pp. 1069- 1073.

8. Omar A. Saldarriaga, Luis E. Perez, Bruno L. Travis, Peter C. Melby. Selective enhancement of type 1 cytokine response by expression of a canine interleukin (IL)-12 fused heterodimemeric DNA. Veterinary Immunology and Immunopathology. 2006 April 15; 110(3-4): 377-88.

9. Omar A. Saldarriaga, Bruno L. Travis, Won Park, Luis E. Perez, and Peter C. Melby. Immunogenecity of a Multicomponent DNA vaccine against visceral leishmaniasis in dogs. Vaccine. 2006 Mar 10; 24(11):1928-40.

10. Luis E. Perez. The lack of Nitric Oxide (NO) production in the hamster (Mesocricetus auratus), which is associated with progressive and lethal Leishmania donovani infection, is due to a defect in the proximal inducible Nitric Oxide Synthase (NOS2) promoter. Thesis Dissertation. University of the Incarnate Word. San Antonio, Texas, May 2003.

11. Anstead GM, Chandrasekar B, Zhao W, Yang J, Perez LE, Melby PC. Malnutrition alters the innate immune response and increases early visceralization following Leishmania donovani infection. Infect Immun 2001, 69:4709-4718. 12. Melby PC, Yang J, Zhao W, Cheng J, Perez LE. Leishmania donovani p36 (LACK) DNA vaccine is highly immunogenic but not protective against experimental visceral leishmaniasis. Infect Immun 2001, 4719-4775.

ACADEMIC ACCOMPLISHMETS

University of the Incarnate Word’s Outstanding Biology Graduate Student Award

(Spring of 2003).

NIH Graduate Student Research Fellowship recipient, 2002-2003 at University of Texas Health Science Center while pursuing my master degree.

Excellence award in athletics and academics, Toledo, Ohio, 1991.

National Honor Society, Toledo, Ohio, 1991.

Outstanding student award, Toledo, Ohio, 1990.

OTHER QUALIFICATIONS

Soccer player.

Completed MS 150 Bike Ride, San Antonio, Corpus Christi, Texas, 2005 & 2006.

I practice yoga on a daily basis.

Salsa Dancer.

Uber Driver.

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