Specialist Ii Drug Safety

Vuong Nguyen, BA, HT (ASCP) CM, QIHCCM

*** ****** ****, **********, ** 03109 Phone: 617-***-**** E-mail: ***********@*******.***

SUMMARY

20+ years of histology experience in both pharmaceutical and hospital settings. Dedicate team-player with a positive attitude with strong organizational and problem-solving skills. Have become the go-to person for last minute high priority projects due to technical expertise and for effectively adapting to last minute changes. Eager to learn or share new skill sets and to take initiatives to broadly expand knowledge in the field.

LABORATORY SKILLS

Microtomy (paraffin, frozen), histology, immunohistochemistry (IHC), in situ hybridization

(ISH) and fluorescence in situ hybridization (FISH), image analysis, laser capture microdissection,

small and large animal necropsy, tissue harvesting and trimming (mouse, rat, hamster, guinea

pig, monkey), whole body perfusion (rodents and NHP), fixation, rodent (rat, mouse) bleeding

techniques (cardiac stick, retro orbital), collection of cerebrospinal fluid (CSF) from mice and rat.

EXPERIENCE

Elliot Hospital, Manchester, NH

Histology Technician, September 7, 2021 – Present

Independently support all routine histology, special stains and IHC as needed to provide high quality microscopic slides

Massachusetts General Hospital (MGH), Boston, MA

Histology Specialist II, January 04, 2021 – September 17, 2021

Prepared a variety of routine and complex tissue from fixation to final microscopic slide

Embedded surgical and autopsy tissue, orienting according to tissue type

Sectioned paraffin blocks of routine and complex tissue from 2 to 50 microns

Performed all other duties and responsibilities as directed

Independently trouble-shoots problems relating to routine operation of laboratory

Extensive knowledge of all laboratory equipment

Participates in continuing education/training sessions sponsored by the Department

Pfizer, Drug Safety Research Development, Andover, MA

Pathology Scientist (R3), October 16, 2009 – July 2, 2017

Performed all aspect of histology and necropsy

Necropsy skills include large (NHP) and small animal (rodents) tissue harvesting, gross lesion identification and entering data using the PDS (Pathology Data System)

Prepared preliminary gross pathology and organ weight data analysis for the pathologists

Proficient with tissue trimming technique of rodent and non-rodent (NHP) species

Histology skills include soft tissue and hard tissue microtomy, operation of manual and automated microtomes, H&E staining using the Leica XL & Ventana Symphony

Research, develop, troubleshoot and validate new procedures and technologies

Performed QC of all slides produced

Performed special stains

Performed IHC using automated method using the Ventana Discovery XT

Provided image analysis support using Image-Pro Plus version 6.3 when required

Performed histology equipment maintenance

Managed archiving of paraffin blocks

Wrote laboratory procedure and contributed to updating or modifying SOP guidelines

Wyeth Research, Drug Safety and Metabolism, Andover, MA

Scientist II Molbio, April 2005 – October 15, 2009

Responsible for the technical procedures utilized in the preparation of microscopic slides including tissue trimming, processing, paraffin & frozen sectioning, H&E and special stains

Proficient with the use of the Cryo-Jane tape transfer system

Supported the In Vivo group with necropsy of small and large animal, tissue harvesting, bone marrow smear preparation, freezing of tissue, perfusion, CSF collection, blood collection using retro orbital, cardiac stick and vena cava)

Performed immunohistochemistry using manual method and automated method using both Biogenix & Nemesis 7200 systems to include optimization of novel antibodies

Performed in situ hybridization, laser capture microdissection, image capture and morphometry using the Image-Pro Plus version 6.3 and confocal microscopy as needed

Assisted with tissue preparation for FACS analysis and application software

Trained new staff with histology, necropsy and tissue harvesting procedures

Performed histology equipment monthly maintenance, archiving of blocks, slides, wet tissues and recycling of xylene and alcohol waste

Brigham and Women’s Hospital, Boston, MA

Histology and Immuno Specialist, April 2005 - December 2006 (part time)

April 2002 - March 2005 (full time)

Provided molecular pathology support performing manual and automated immunohistochemistry procedures using the Dako systems on paraffin and frozen tissues on both human and diverse animal tissue samples to include optimization of Novel antibodies. Other responsibilities included performing fluorescence in situ hybridization for DNA, tunel assay stains, isolation of DNA and RNA from tissue sections, PCR purification, fixation of cell pellets for impox, preparation of LB medium for plate and cell culture. In addition, performed occasional routine histology to include sectioning of paraffin/frozen section of human/rodent tissues and special stain procedures

Beth Israel Deacon Medical Center, Boston, MA

Histotechnologist, May 2005 - June 2008 Per Diem

Set up and organized a small histology lab in a radiology department for human prostatic research. During this period, I supported with embedding, sectioning and staining whole mount prostates

Harvard Vanguard Medical Associates, Boston, MA

Histotechnician, 2004 - 2005 Per Diem

Performed routine histology to include tissue embedding, sectioning and immunohistochemistry using automated Ventana systems

Ardais Corporation, Lexington, MA

Laboratory Support Specialist, August 2001 - March 2002

Provided histology support with tissue trimming, processing, embedding, and some paraffin and frozen sectioning. Other duties included equipment QC and maintenance, autoclaving, routine staining, coverslipping, inventory control, special sample handling and shipping of samples

EDUCATION

2008-2009 Temple University - School of Pharmacy, PA

Graduate Certificate for Drug Development Program in QA/RA

1996-2001 Saint Anselm College, Manchester, NH

Bachelor of Art in Natural Science

CERTIFICATIONS

ASCP Board of Registry Histotechnician #19500 (2004 - present)

ASCP Qualification in Immunohistochemistry (2013- present)

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