Shammi Akter

Delaware Biotechnology Institute, University of Delaware

*** ****** ***, ******, ** 19713, USA

Mobile: 302-***-****, ad4lh7@r.postjobfree.com

https://www.linkedin.com/in/shammi-akter-ph-d-989467194/

PERSONAL STATEMENT

Ph.D. with 5+ years’ experience in Cellular system biology lab with experience in synthetic biology, molecular biology, recombinant therapeutic protein expression, purification & activity assay

2 years excellent experience in Pharmaceutical Industry in aseptic technique & GMP/GLP regulation

Very good analytical and experimental skills to execute adequately in new projects and meet tight timelines

I have a positive attitude, excellent communication skills, and the ability to establish and maintain positive working relationships with others. I am capable of working independently as well as collaboratively as part of a team.

EDUCATION

Pohang University of science and Technology

Ph.D. Life Science Department [Pohang, South Korea: 09/2019-02/2023]

State University of Bangladesh

Masters of Pharmacy [Dhaka, Bangladesh: 01/2012-03/2013]

Manarat International University

Bachelor of Pharmacy [Dhaka, Bangladesh: 09/2007-08/2011]

RESEARCH EXPERIENCE

Delaware Biotechnology Institute

Postdoctoral Researcher, Synthetic Biology Lab [Newark, DE, USA: 07/2023-Present]

Research Focus: Synthetic reprogramming of plant developmental and biochemical pathways.

Ultimate aim is to address challenges in agriculture & human health.

Pohang University Science and Technology

Graduate Research Assistant, Cellular system biology lab [Pohang, South Korea: 09/2019-02/2023]

Research Focus: Production & development of numerous recombinant protein using plant base expression systems. Particularly focuses on the development of cost-effective purification of biopharmaceutical/enzyme protein in plants to improve global access to modern medicines.

Pohang University Science and Technology

Researcher, cellular system biology lab [Pohang, South Korea: 09/2018-08/2019]

INDUSTRIAL EXPERIENCE

Apex pharma Ltd.

Executive Quality Control [Kaliakoir Gazipur Bangladesh: 01/201*-**-****]

Job Responsibilities

To ensure the quality of raw materials, packaging materials and finished products are compliance with the approved specifications GMP/GLP

Maintains documentation in the quality control department

To perform calibration laboratory equipment

To prepare STP for FDF & Raw materials & prepare new SOPs, revision & update of existing SOP's

Quality decisions based on GMP and site SOPs/documentation

Sampling, inspections and testing as per specifications of raw materials

To perform stability study/Re-test of raw materials

Collection of samples of processed products & finished products (tablet capsules, liquids and injections) for final approval or rejection

Instrument use:

High performance liquid chromatography (HPLC Prominence-i LC230c 3D, Prominence-LC 20AT Shimadzu), Size exclusion chromatography (FPLC AKTA purifier 10), Atomic adsorption spectrometer AA-6300 Shimadzu, Karl-Fisher (Metrohm 888), FTIR Spectrophotometer (Shimadzu), Refractometer, Polarimete, Viscometer, UV-Vis Spectrometer (UV 1800 Shimadzu)

Light, Bright field, fluorescent and confocal microscopy (Leica Stellaris)

MOLECULAR BIOLOGY LABRORATORY SKILLS

Molecular cloning (Gibson assembly, HiFi assembly, Restriction enzyme digestion) for vector design

PCR Primer design for the addition or dilation or amino acid sequence

RT-qPCR for transcript level analysis

RNA & DNA isolation

Bacterial, Agrobacterium cell culture for recombinant protein expression in plant system

Mouse cell (MIN6m9), Mammalian cell (hIR) overexpression human insulin receptor cell culture for the analysis of activity plant produced recombinant protein

SDS-PAGE, Western Blot for confirmation of protein expression

Biochemical assays: Recombinant protein expression and purification by Ni-NTA resin, Protein A resin, MCC beads (Micro-Crystalline Cellulose) in E. coli (BL21DE3) for purification of target protein

Molecular pharming for scale up recombinant protein, Plant Protein engineering,

A new laboratory set up, experience on purchasing laboratory reagent and instrument

Software use

Geneious Prime, Graphpad Prism, ImageJ, Origin 2022, Adobe Photoshop, Adobe illustrator, Microsoft Word, Excel, and PowerPoint

PUBLICATION

Patent

Shammi Akter, Joohyun Kang, Areum Yoon, Seungjin Woo, Jaeyoon Kim, Inhwan Hwang (2022) Method of insulin production using new recombinant gene construction in plants and its use. (Korean patent applied). (POSTECH No.: P2022344-01-KR)

Shammi Akter, Jaeyoon Kim, Joohyun Kang, Mehrnaz Entesari, Inhwan Hwang (2022) Recombinant fusion gene construction for production of bovine-derived platelet-derived growth factor BB recombinant protein and method of producing the recombinant protein in plants. (Korean patent applied). (POSTECH No.: P2022345-01-KR)

Article

Shammi Akter, Shajia Afrin, Jaeyoon Kim, Joohyun Kang, Md Abdur Razzak, Per-Olof Berggren, Inhwan Hwang (2022) Production of active Exendin-4 in Nicotiana benthamiana and its application in treatment of type-2 diabetics. Front. Plant Sci. doi: 10.3389/fpls.2022.1062658.

Shammi Akter, Jaeyoon Kim, Joohyun Kang, Inhwan Hwang (2023) A novel approach for the production of active Insulin in Nicotiana benthamiana and its application in treatment of type-2 diabetics. (Manuscript in preparation).

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