NINA M. WOLF, PhD

Chicago, IL *****

630-***-****

ad4clp@r.postjobfree.com

https://www.linkedin.com/in/nina-wolf-009a476/

https://www.researchgate.net/profile/Nina-Wolf-4

SUMMARY OF QUALIFICATIONS

• 9+ years of experience in biochemistry and research science with a focus on protein chemistry

• Extensive experience in molecular biology, protein engineering, protein purification, protein characterization, protein structure determination and protein-ligand interaction studies

• Mentored and trained junior scientists and colleagues in protein purification and characterization

• Collaborated on interdisciplinary teams including industry, academia and a nonprofit agency to develop novel protein inhibitors, immunological assays, entheogenic treatments and plant-based nutrition

• Possess strong analytical, organizational, leadership, written and oral communication skills WORK EXPERIENCE

Abbott, Abbott Park, IL 2022 – Present

Postdoctoral Scientist

• Protein engineering for immunological assays to provide proteins of sufficient potency and stability

• Express proteins in E. coli, Pichia pastoris and mammalian cells for purification

• Design and execute purification schemes with manufacturability

• Present scientific progress internally and engage with assay development team to improve biologic characteristics

• Design proteins for improved stability and structure using AI generative tools Back of the Yards Algae Sciences, Chicago, IL 2021 – 2022 Director of Biochemistry

• Analyze entheogenic molecules in fungal extracts by use of HPLC and mass spectrometry

• Complete purification of psychoactive compounds from fungal extract by HPLC

• Perform genomic fungal DNA extraction in various species for sequencing

• Perform analysis of algae extracts for various analytes and protein concentration

• Prepare scientific literature and grant proposals Chicago Recovery Alliance, Chicago, IL 2020 – 2021 Controlled Substances Analyst

• Employed Fourier transform infrared spectroscopy and high-pressure mass spectrometry to test trace samples for composition analysis for a harm reduction nonprofit Institute for Tuberculosis Research, UIC, Chicago, IL 2015 – 2021 Research Scientist

• Optimized protein purification and enzymatic assays for high throughput screening

• Employed secondary screening and lead hit confirmation

• Utilized biophysical methods such as surface plasmon resonance and X-ray crystallography to study atomic level interactions between protein and drug-like macrocyclic peptides

• Worked in collaboration with a full set of drug discovery methods to optimize a drug target campaign

• Sequenced Mycobacterium tuberculosis strains to confirm mutation sites

• Analyzed genomic DNA of non-tuberculosis Mycobacteria and ESKAPE pathogens for identification purposes and to determine mutations in target gene

Department of Chemistry, UIC 2008 – 2014

Research Assistant

• Employed molecular cloning techniques to construct bacterial plasmids and perform mutagenesis studies

• Purification of proteins from bacterial expression system using affinity chromatography

• Characterization of protein for biophysical properties such as secondary, tertiary and quaternary structure, hydrodynamic size and thermal stability

• Characterization of protein ligand interactions using X-ray crystallography, isothermal titration calorimetry, enzyme inhibition assay, and thermal shift assay by qPCR for binding

• Development and optimization of a high-throughput thermal shift assay for small molecule screening EDUCATION

Post-Doctoral Research at the Institute of Tuberculosis Research, College of Pharmacy, University of Illinois at Chicago Doctor of Philosophy in Chemistry with Biochemistry concentration, Department of Chemistry, College of Liberal Arts

& Sciences, University of Illinois at Chicago

SPECIALIZED TRAINING

Protein techniques: Protein expression and purification in bacteria and yeast, Fast protein liquid chromatography

(FPLC), Surface plasmon resonance (SPR), X-ray crystallography, Isothermal Titration Calorimetry (ITC), Dynamic Light Scattering (DLS), Protein electrophoresis, Size-exclusion chromatography (SEC), Circular dichroism (CD), Fluorescence spectroscopy, UV spectroscopy, Nuclear magnetic resonance (NMR), Mass spectrometry (MS), Western blot, Sucrose gradient ultracentrifugation, Protein engineering, AI protein design DNA techniques: Plasmid and genomic DNA extraction, qPCR, PCR, Cloning, Site-directed mutagenesis, Gene sequence analysis, DNA electrophoresis, Sanger sequencing Analytical techniques: Mass spectrometry (MS), High pressure liquid chromatography (HPLC), Fourier transform infrared spectrometry (FTIR)

PUBLICATIONS

New Rufomycins from Streptomyces atratus MJM3502 expand anti-Mycobacterium tuberculosis structure activity relationships. B Zhou, G Shetye, NM Wolf, S-N Chen, M Qader, GJ Ray, D Lankin, S-H Cho, J Cheng, J-W Suh, SG Franzblau, J McAlpine, GF Pauli. (2022) Organic Letters 24: 7265–7270. Structure of the N-terminal domain of ClpC1 in complex with the antituberculosis natural product ecumicin reveals unique binding interactions. NM Wolf, Hyun Lee, D Zagal, J-W Nam, D-C Oh, Hanki Lee, J-W Suh, GF Pauli, S Cho, C Abad-Zapatero. (2020) Acta Crysallogr D76: 458–471. Antimycobacterial rufomycin analogues from Streptomyces atratus strain MJM3501. B Zhou, G Shetye, Y Yu, BD Santarsiero, LL Klein, C Abad-Zapatero, NM Wolf, J Cheng, Y Jin, H Lee, J-W Suh, H Lee, J Bisson, JB McAlpine, S-N Chen, S-H Cho, SG Franzblau, GF Pauli. (2020) Journ Natural Products 83: 657-667. Structural and biochemical characterization of the class II fructose-1,6-bisphosphatase from Francisella tularensis. AI Selezneva, HJ Gutka, NM Wolf, F Qurratulain, F Movahedzadeh, C Abad-Zapatero. (2020) Acta Crysallogr F76:524-535.

Expression, purification, and crystallization of Rv0100, a proposed acyl carrier protein of Mycobacterium tuberculosis. JMG Bondoc, HJ Gutka, MM Almutairi, R Patwell, MW Rutter, NM Wolf, R Samudrala, S Mehboob, F Movaheadzadeh.

(2019) Acta Crysallogr D75: 646-651.

High-resolution structure of ClpC1-Rufomycin and ligand binding studies provide a framework to design and optimize anti-tuberculosis leads. NM Wolf, Hyun Lee, MP Choules, GF Pauli, R Phansalkar, JR Anderson, W Gao, J Ren, BD Santarsiero, Hanki Lee, J Cheng, Y-Y Jin, NA Ho, N M Duc, J-W Suh, C Abad-Zapatero, S Cho. (2019) ACS Infect Dis 5: 829–840.

Rufomycin targets ClpC1 proteolysis in Mycobacterium tuberculosis and M. abscessus. MP Choules, NM Wolf, Hyun Lee, JR Anderson, EM Grzelak, Y Wang, R Ma, W Gao, JB McAlpine, Y-Y Jin, J Cheng, Hanki Lee, J-W Suh, NM Duc, S Paik, JH Choe, E Jo, CL Chang, JS Lee, BU Jaki, GF Pauli, SG Franzblau, S Cho. (2019) Antimicrob Agents Chemotherapy 63: e02204–18.

Structures of the Mycobacterium tuberculosis GlpX protein (class II fructose-1,6-bisphosphatase): Implications for the active oligomeric state, catalytic mechanism and citrate inhibition. NM Wolf, HJ Gutka, F Movaheadzadeh, C Abad Zapatero. (2018) Acta Crysallogr D74: 321–331.

Mutagenesis of threonine to serine in the active site of Mycobacterium tuberculosisfructose-1,6-bisphosphatase

(class II) retains partial enzyme activity. JMG Bondoc, NM Wolf, M Ndichuck, C Abad-Zapatero, F Movaheadzadeh.

(2017) Biotechnol Rep 15: 48-–54.

Enzymatic characterization of fructose 1,6-bisphosphatase II from Francisella tulerensis, an essential enzyme for pathogenesis. HJ Gutka, NM Wolf, JMG Bondoc, F Movaheadzadeh. (2017) Appl Biochem Biotechnol 4: 1439–1454. Identification of B. anthracis N5-carboxyaminoimidazole ribonucleotide mutase (PurE) active site binding compounds via fragment library screening. H Lei, C Jones, T Zhu, K Patel, NM Wolf, LW-M Fung, H Lee, ME Johnson. (2016) Bioorg Med Chem 4: 596–605.

Identification of Bacillus anthracis PurE inhibitors with antimicrobial activity. A Kim, NM Wolf, T Zhu, ME Johnson, J Deng, JL Cook, LW-M Fung. (2015) Bioorg Med Chem 23: 1492–1499. Differences in the purification and properties of PurC gene products from Streptococcus pneumoniae and Bacillus anthracis. ML Tuntland, NM Wolf, LW-M Fung. (2015) Protein Express Purif 114: 143-148. Structures of SAICAR synthetase (PurC) from Streptococcus pneumonia with ADP, Mg2+, AIR and Asp. NM Wolf, C Abad Zapatero, ME Johnson, LW-M Fung. (2014) Acta Crystallogr D70: 841-850. Site-directed mutagenesis of N5-carboxyaminoimidazole ribonucleotide mutase (class I PurE) in Bacillus anthracis. M Silas, NM Wolf, LW-M Fung. (2012) Undergrad Journ Res 5: 33-37. AWARDS AND FELLOWSHIPS

Post-Doctoral Association Travel Award, UIC, Chicago, Illinois 2019 Protein Society Travel Award, Protein Society 32nd Annual Symposium, Boston, Massachusetts 2018 High-Throughput Screening Award/Grant, Chicago Biomedical Consortium, Chicago, Illinois 2016 SENCER SCI-Midwest Implementation Award, UIC, Chicago, Illinois 2015 College of Pharmacy Image Competition, 1st Place Winner, UIC, Chicago, Illinois 2015 Chancellor’s Award Fellowship for Interdisciplinary Research, Ph.D. Research, UIC, Chicago, Illinois 2013 & 2014

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