Laura Mendoza

e:ad45r5@r.postjobfree.com c: 415-***-****

Currently seeking employment. I have experience with PCR techniques, Cell based assays such as ELISA, MSD, Luminex, Tissue Culture, RNA from tissue isolations, cDNA production, gDNA Isolations, qPCR, Maxi Prep and cell line construction and development. My goal is to acquire and maintain work that provides learning new techniques in research.

Education

M.S. Biology (Cell and Molecular Emphasis), San Francisco State University, May 2010

B.S. Biology, (Cell and Molecular Emphasis), San Francisco State University, August 2001

Pharmacy Technician Certification, Valley Medical Center, Fresno, CA January 1990

Work Experience

DC3 Therapeutics Jan 2023 - Current

Research Associate

PCR Techniques

End Point, Primer design (Genius), Mycoplasma, cDNA generation, qPCR

Maxi Prep

Introduction of plasmids into bacterial cells and gDNA extraction

Fragment Analysis

Used to identify Cell strains with CLA software

Tissue Culture

Aseptic technique, Media prep., Propagation of Huvec and HEk 293 Cells, Cell banking

Nucleic Acid Isolation

RNA and gDNA from heart, liver or brain tissue and Cells

Equipment Maintenance

Surface cleanings, preventative maintenance

Assay Development

Mycoplasma PCR Diagnostic

MSD Chemo Electric signaling

Sample preparation, execution of assay and analysis

Luminex Magnetic Bead with fluorescence signaling

Sample preparation, Execution of assay and analysis

Construction of protein expressing cell lines

PPARA in HEK cells

Astellas Gene Therapy Jan2022 – Dec 2022

Manufacturing Associate I

Upstream production of cells for gene therapies

Set up and operation of 200L and 500L Bio reactors

Follow SOPs as well as GDP/GMP

Use aseptic technique for Cell Expansion and Transfection SOPs

Use of NOVA Bio Flex for sample analysis

Buffer Preparation

Monthly and biweekly Equipment Cleanings

Used Oracle to systematically order from warehouse

Log Book documentation

Baxter Healthcare May 2021 - Dec 2021

Manufacturing Technician I

Support manufacturing process and operate general production equipment.

Perform and maintain GDP and GMP guidelines

Receive and distribute supplies

Prepare and package products for shipment

Formulate Coseal cross link

Perform cleaning of equipment small parts and clean room

Calculate batch and perform GDP on batch records upon production

Impossible Foods July 2019 - September 2020

Data Integrity/Machine Operator, Impossible Foods

Data entry

Print and completed work orders

Moved materials systematically

Located missing lots

Consolidated materials at end of production batches

Ensure data integrity

Operated OSC machine

Ran x-ray checks and film checks on packaging

Acquired and prepare samples for QA

Practiced GDP, GMP and LEAN thinking

Noah's Bagels March 2017 - January 2019

Shift Lead

Managed shift

Adhered to food and safety protocols

Prepared cashier drawers at end of day

Make Bank deposits

Adhered to food and safety protocols

Prepare cashier drawers at end of day

Ensure Cleaning is conducted prior to closing

Hope Net July 2019 - September 2020

Green House Manager

Initiated frequency studies for day/night growth using 5-10 hertz

Trained technicians to create clones

Optimized plant growth by insuring nutrients and tanks are at acceptable pH levels

Developed natural pesticide solutions for powdery mildew, root rot, spider mites and other pests

USDA ARS WRRC PSM Unit August 2003 - May 2010

Graduate Student Research

Conducted independent research on the molecular characterization of campylobacter mu like phage using PCR to identify mu transformase protein A from different encoded CMLP campylobacter species

Carried out cell culture for campylobacter chemical induction of putative mu like phage along with controls

Provided evidence of mu like phage presence, viability, and visualization by transmission electron microscopy

Received and maintained bacterial specimen for strain collection and inputted the data into USDA’s electronic database

Performed quality control protocols on lab equipment

Wrote independent protocols for campylobacter mu like phage research

Presented research at conferences, lab meetings and poster presentations

San Francisco State University August 2001 - June 2002

Graduate Student Research Associate

Conducted independent research in the cloning Y4IO from Rhizobium into tomato plant

Learned to make solutions and media

Carried out cell culture, tomato propagation, protein analysis, PCR, DNA sequencing and analysis

Presented research at conferences, lab meetings and poster presentations

San Francisco State University September 2000 - August 2001

Undergraduate Student Research Associate

Conducted independent research on transformation efficiency in castor plat

Performed vector analysis and constructed GUS expression vector to be used for resin knock out

Propagated castor plants, castor beans and cell cultures to be used in experiments

Presented research at conferences, lab meetings and poster presentations

USDA-ARS-WRRC CIP Unit June 2000 - August 2000

Summer Internship

Conducted independent research on cloning Tri12 gene from Fusarium into wheat plant

Performed DNA analysis for cloning Fusarium Tri12 and transformed construct into wheat plants

Learned and used DNA bombardment, callus production for wheat micro propagation

Verified presence of Fusarium Tri12 using PCR analysis and DNA

DNA analysis

Propagated and maintained different strains of tomatoes and cell cultures for lab experiment use

Presented research at conferences, lab meetings and poster presentations

San Francisco State University June 1998 - August 2000

Undergraduate Student Research Associate

Conducted independent research on molecular genetic dissection of AvrRxv function by basic molecular techniques as PCR, DNA extraction, gel electrophoresis, cell culture and DNA analysis

Propagated and maintained different strains of tomatoes and cell cultures for lab experiment use

Presented research at conferences, lab meetings and poster presentations

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