Ilia Fishbein, M.D., Ph.D.

Scientific Director - Profile

Principal Scientist

Lead Scientist

Philadelphia, PA • 215-***-****

ad3xzq@r.postjobfree.com

https://www.linkedin.com/in/ilia-fishbein-363b472/

Qualifications Summary

Doctor of Medicine and Pharmaceutical Science with extensive research experience in the university environment. Broad expertise across animal models of cardiovascular diseases, endovascular interventions, restenosis, atherosclerosis, gene therapy, cell therapy, ad/AAV vectors, nitric oxide and reactive oxygen species physiology and pharmacology, drug and gene delivery systems, biomaterials, bioconjugation chemistry, optical imaging, histology, immunohistochemistry and immunofluorescence techniques, fluorescence microscopy, pathology, cell culture techniques, and assay development. Guides the design and implementation of biomedical experiments to contribute innovative advancements in the field.

Core Competencies

Cardiovascular Disease

Gene/Cell Therapy

Drug/Gene Delivery Systems

Animal Models

Immunohistochemistry

Assay Development/ELISA

Controlled Release

Endovascular Interventions

Ad Vectors/AAV Vectors

Biomaterials/Biopharmaceutics

Optical Imaging

Fluorescence Microscopy

Biomedical Experiments

Magnetic Targeting

Restenosis/Atherosclerosis

Physiology/Pharmacology

Bioconjugation Chemistry

Histology/Pathology

Macrophage Biology

Cell Culture Techniques

Project Design/Management

Education

Doctor of Medicine School of Medicine, University of Saratov, Saratov, USSR

Doctor of Philosophy in Pharmaceutical Science Hebrew University of Jerusalem, Jerusalem, Israel

Certification

Educational Commission for Foreign Medical Graduates (ECFMG) Certificate, USA

General Medical Practice, Israel

Licensure

Licensing Council, Israeli Ministry of Health, active

Career Experience

School of Medicine University of Pennsylvania, Philadelphia, PA 2015 – Present

Research Associate Professor of Pediatrics

Conducted research on:

Chemical modification and fluorescent labeling of Ad, AAV, and lentiviral vectors;

Developing a magnetic delivery/targeting catheter and characterizing it in the porcine femoral model;

Macrophage biology in vasculo-proliferative diseases;

Elucidating the role of macrophage polarization in in-stent restenosis;

Developing AAV-eluting stents;

Comparing the long-term effects of permanent metal stents and biodegradable polymeric stents on arterial remodeling and restenosis;

Improved histological processing of stented arteries (tissue-sparing chemical stent dissolution);

Developing a software for automatic analysis of microscopic images;

Surface modification of Ad and AAV vectors with proteolytic enzymes for enhanced vector spread through solid tumors;

AAV-based gene delivery of an oxidation-resistant apoA1 variant (apoA1-4WF) for the prevention of restenosis in the porcine coronary model;

The anti-atherosclerotic and anti-restenotic effects of blood outgrowth endothelial cells transduced with wild-type apoA1 and apoA1-4WF;

Accelerated reendothelialization of endovascular stents using the enhanced attachment of biotinylated endothelial cells to avidin-functionalized stents;

Stent functionalization with CD47 and CD47-derived peptides for the prevention of in-stent restenosis in the rabbit iliac model.

Authored and published 25 research studies, six reviews/book chapters, and 31 conference abstracts.

Mentored four postdoctoral fellows, a graduate cell and molecular biology student, one graduate pharmacology student, and five undergraduate bioengineering students.

Served as an external examiner for a doctoral thesis.

Served as a reviewer of 73 papers.

Presented research at five invited presentations.

Research Assistant Professor of Pediatrics, School of Medicine 2005 – 2015

Conducted research on:

Chemical modification of Ad vectors;

Methods of Ad vector purification;

Reversible immobilization of non-viral gene vectors and Ad vectors to the surfaces of bioimplants;

Use of protein transduction domains for transduction enhancement with Ad vectors;

Use of multifunctional polymers for photoactivatable conjugation to the surface of PLA/PLGA nanoparticles and gene vectors;

Developing anti-knob antibody-based and CAR-D1-based adaptors for targeted delivery of Ad vectors;

Magnetic field-facilitated delivery of Ad/magnetic nanoparticle complexes in vitro and in vivo;

Developing gene-eluting stents;

Bioluminescence-based detection of reactive oxygen species in the stent-injured vessel wall;

Developing a new rat model of carotid artery stenting and adapting it for use in gene-eluting stent studies.

Authored and published 16 research papers, four reviews/book chapters, and 18 conference abstracts.

Courses taught include Principles of Cardiovascular Biology and a seminar on Cardiovascular Gene Therapy.

Mentored four postdoctoral fellows, two graduate pharmacology students, one medical student, and one undergraduate bioengineering student.

Presented research at seven invited presentations.

Served as a reviewer for 52 papers.

Previous Experience

Postdoctoral Fellow • Division of Cardiology, Children's Hospital of Philadelphia

Reservist Service Member, Medical Corps • Israel Defense Army, Israel

Staff Physician, Department of Cardiology • Hospital #26, Kiev, Ukraine

Staff Physician, Department of Cardiology • Central Hospital #6, Penza, Russia

Intern in Medicine • Central Hospital #6, Penza, Russia

Honors & Awards

Innovation Award, Stokes Fellows Research Poster Day, Children's Hospital of Philadelphia

Goldhaber Family Award for Academic Achievements, Hebrew University of Jerusalem

The Nagai Foundation Graduate Student Award, Controlled Release Society

Grants

Pending

Role: Primary Investigator, Stent surface modification with programmable hydrolysable polymers for prevention of restenosis, NIH/NIBIB, July 2023 – June 2027, $499,412, 40% effort

Role: Primary Investigator, Stent surface modification with programmable hydrolysable polymers for prevention of restenosis, AHA, Collaborative Sciences Award, July 2023 – June 2026, $250,000, 30% effort

Role: Primary Investigator, AAV-IL10 platelet membrane cloaking for enhanced vascular wall transduction and restenosis mitigation, AHA, Innovative Project Award, July 2023 – June 2025, $100,000, 25% effort

Role: Primary Investigator, Enhanced in situ stent endothelialization via biotin/avidin affinity coupling, NIH/NHLBI R01, April 2023 – March 2027, $361,314, 50% effort

Current

Role: Co- Primary Investigator, Oxidation Resistant ApoA1 Gene Delivery Stents, NIH/NHLBI, 1R01HL137762, July 2017 – June 2023, $627,879, 40% effort

Past

17 grants – full details available upon request

Patents

U.S. Patent # 9,694,103, Photo-chemical activation of surfaces for attaching biomaterial. Alferiev, Fishbein, Chorny, Levy, 2017.

U.S. Patent # 9,095,610 B2, Uniform Field Magnetization and Targeting of Therapeutic Formulations. Levy R, Polyak B, Chorny M, Fishbein I, Alferiev I, Friedman G, Williams D., 2015.

U.S. Patent # 9,084,877, Magnetic targeting device, system and method. Levy, Chorny, Fishbein, 2015.

U.S. Patent # 9,084,877 B2, Magnetic targeting device, system and method. Levy, Chorny, Fishbein. 2015.

U.S. Patent # 8,562,505 B2, The Children's Hospital of Philadelphia: Uniform field magnetization and targeting of therapeutic formulations. Alferiev IS, Chorny M, Fishbein I, Friedman G, Levy RJ, Polyak B, Williams D., 2013.

U.S. Patent # 8,580,545, The Children's Hospital of Philadelphia: Biodegradable linkers for molecular therapies. Alferiev IS, Fishbein I, Levy RJ, 2013.

U.S. Patent # provisional application RCHP-195USP, The Children's Hospital of Philadelphia: Ex vivo-modified monocytes as local delivery vehicles to treat diseased arteries. Fishbein I, Levy RJ, 2011.

U.S. Patent # 7,846,201, The Children's Hospital of Philadelphia: Magnetically-driven biodegradable gene delivery nanoparticles formulated with surface-attached polycationic complex. Chorny M, Polyak B, Fishbein I, Alferiev IS, Levy RJ, 2010.

U.S. Patent # 7,589,070, The Children's Hospital of Philadelphia: Surface modification for improving biocompatibility. Levy RJ, Alferiev IS, Song C, Fishbein I, 2009.

U.S. Patent # 7,635,734, The Children's Hospital of Philadelphia: Photochemical activation of surfaces for attaching biomaterial. Alferiev IS, Fishbein I, Chorny M, Levy RJ, Yellen BB, Williams D, 2009.

U.S. Patent # 6,890,998, The Children's Hospital of Philadelphia: Thiol activation of polyurethanes and methods of making the same. Alferiev IS, Fishbein I, Levy RJ, 2005.

U.S. Patent # 6,900,282, The Children's Hospital of Philadelphia: Derivatized polyurethane compositions which exhibit enhanced stability in biological systems and methods of making the same. Alferiev IS, Fishbein I, Levy RJ, 2005.

Patent # EP10948220B1, The Hebrew University of Jerusalem: Use of bisphosphonates in the treatment of vascular restenosis. Golomb G, Danenberg HD, Monkkonen J, Fishbein I, 2004.

U.S. Patent # US20040063158A1*, The Children's Hospital of Philadelphia: Methods of determining surface binding capacity. Alferiev IS, Fishbein I, Levy RJ, 2004.

U.S. Patent # WO02103004A1*, The Children's Hospital of Philadelphia: Surface modification for improving biocompatibility. Alferiev IS, Fishbein I, Levy RJ, 2002.

Committees

Voting Member, Executive Board, Clinical and Scientific Advisory Committee (CSAC), Pennsylvania Pediatric Device Consortium (PPDC), 2018 – Present

Member, CSAC, PPDC, 2013 – Present

Organizing Committee Member, 3rd International Conference on Translational Medicine, Las Vegas, NV, 2014

Voting Member, Scholarship Oversight Committee, Division of Cardiology, Children's Hospital of Philadelphia, 2010 – Present

Editorial Board Positions

Editorial Board Member, Angiology, 2013 – Present

Editorial Board Member, Journal of Functional Biomaterials, 2021 – Present

Associate Editor, Frontiers in Cardiovascular Research, 2023 – Present

(Reviewer of numerous scientific journals, full details available upon request)

Publications & Presentations

48 peer-reviewed research publications

1 non-peer reviewed research publication

10 peer-reviewed reviews of research publications

59 abstracts

7 chapters, editorials, reviews, and committee reports

12 invited lectures

Full details available upon request

Affiliations

Member, American Society of Gene and Cell Therapy

Member, American Heart Association

Languages

English, Hebrew, Russian, Ukrainian

Technical Proficiencies

Laboratory Techniques: Cell culture, transfection, transduction, RT-PCR, Western blot, ELISA, spectrophotometry, fluorimetry, luminometry, chromatography, biochemical assays

Software: Microsoft Office, EndNote, GraphPad, Imaging and image analysis software (NIH Image, Image J, Living Image, Photoshop), SnapGene, SoftMax Pro

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