FIELD OF STUDY:

* ******** *** ****** ******** Trials: serving as a Research Scientist and Coordinator focusing on AI-supported genomic data analysis to match the driver mutations of EU cancer patients and appropriate US clinical/research trials, - leading collaborative efforts at DNAi -The Center of Genetic Information and other precision medicine companies, New York, US, 05/2023-present. 2 Cancer research: Research Scientist, Postdoctoral associate at The State University of New York USA, 01/2018 -05/2023 3 Molecular biology, cancer cell biology, M.Sc., 06/2001 4 Chemical technology, Light organic technology, biotechnology of recombinant proteins, Eng., 10/2004 5 Laboratory diagnostics, license to practice the profession, 03/2003 6 Forensic Sciences, court expert in genetics and biochemical identification, 11/2008 7 General nursing, license to practice the profession, 07/1995 PROFESSIONAL INTERESTS: 1 Translational control in human diseases - mechanistic studies, 2 Alternative mechanisms of translation initiation switched by integrated stress response (IRS), 3 Non-coding RNA, dsRNA and Z-RNA in cellular senescence, apoptosis, inflammation, and immune response, 4 Precise gene expression regulation: gene-specific enhancement of protein translation of selected tumor suppressors, gene silencing and genome editing, CRISPR-Cas9, 5 Molecular medicine, molecular diagnostics, targeted gene therapy, next generation therapeutics, nucleic acid-based therapeutics and biotechnology of recombinant fusion proteins, 6 Pharmacogenomics, big data analysis, genetics, transcriptomics, proteomics, bioinformatics, -omics, EDUCATION BACKGROUND

Date Academic

degree/title

Position/

function University/school/lab Field of study/work 2023 -2023 Research

Scientist

The State University

of New York (SUNY), USA

Cancer research, genomics,

transcriptomics, neuro-

inflammation

2018

-2023 Postdoctoral Postdoctoral

Associate

The State University

of New York (SUNY), USA

Cancer research, cancer

prevention, DED, inflamma-

tion, bioinformatics, -omics

2010

-2016 Dr med. sc.

(doctor of med.

science, not

physician)

Research

Scientist.

The Center of Postgraduate Medical

Education in Warsaw, Poland

and DNAi biotech lab

Cancer research projects,

grants, papers followed by

PhD public defense.

2005 -2010 PhD student The Center of Postgraduate Medical Education in Warsaw, Poland

Medical biology PhD program,

cancer research

2001

-2004 Eng. Student Krakow University of Technology, Poland

Chemical light organic

technology and engineering

1996 -2001 M.sc. Student Jagiellonian University, Krakow, Poland Molecular biology, cell biology, genetics

1990 -1995 student Nursing high school in Wadowice, Poland

General nursing,

medical projects

1991 -1994 Student Music school in Wadowice Music instruments, saxophone dr med. sc. ADAM MASTER

Address: 65 Tompkins Ave, 2H

Brooklyn, New York, 11790

ad3thz@r.postjobfree.com

tel.: +1-516-***-****

http://www.gene-factory.com

ORCID: 000*-****-****- 8846

Scientist

Inventor

Researcher

22 yrs exp.

Research Scientist and Postdoctoral associate:

The State University of New York (SUNY)

Genetic analysis and bioinformatics:

DNAi - The Centre of Genetic Information in Krakow Born on the 18th of January 1975

in Wadowice, Poland

Languages:

English (USA, NY, 6 years)

Polish (native)

Curriculum Vitae

academia & industry

SCIENTIFIC EXPERIENCE

2023-present. Research Scientist and Coordinator at DNAi-The Center of Genetic Information, leading EU-US collaborative efforts to recruit cancer patients for appropriate clinical/research trials based on their driver mutations. My responsibilities include AI-supported 1) molecular diagnostics focusing on genomic signatures, 2) identifying targeted therapy options as part of precision medicine, and 3) searching for clinical trials as listed on e.g. clinicaltrials.gov (NIH).

2023-2023. Research Scientist at the Department of Family, Population & Preventive Medicine, Division of Cancer Prevention, SUNY at Stony Brook Medicine (SBM), specializing in cancer research, with a particular focus on the molecular aspects of paclitaxel- induced neuropathic pain (PIPN) in a mouse model. Conducting mouse transcriptome analysis, exploring neuronal cell differentiation, and investigating axonal transport in cell cultures. 2021-2023. Postdoctoral Associate at the Department of Family, Population & Preventive Medicine, Division of Cancer Prevention, SUNY at Stony Brook Medicine (SBM), NY, specializing in research and innovations spanning cancer genetics, bioinformatics, high-throughput next-generation technologies, cell cultures, animal models, Patient-Derived Xenografts (PDXs), chemotherapy-induced peripheral neuropathy, neuronal differentiation, inflammation, immune response in dry eye disease, improved ocular impression cytology, quantitative cell transfer to microscope slides, and ex-vivo cultures of conjunctival cells on nitrocellulose scaffolds.

2020-2021. Postdoctoral Associate at the Department of Ophthalmology, Division of Cancer Prevention, SUNY at Stony Brook Medicine

(SBM), specializing in technical innovations for research on the molecular aspects of dry eye disease. Investigating inflammation and immune responses in dry eye disease. Proficient in cancer genetics, bioinformatics, high-throughput next- generation technologies, transcriptome analysis, cell cultures, animal models, and Patient-Derived Xenografts (PDXs), in vitro and in vivo combination therapy.

20018-2020. Postdoctoral Associate at the Department of Medicine, Division of Cancer Prevention, SUNY at Stony Brook Medicine

(SBM), specializing in research and inventions encompassing cancer genetics, bioinformatics, high-throughput next- generation technologies, drug synthesis, cell cultures, and animal models, including Patient-Derived Xenografts (PDXs). Conducting analyses of kinome and large-scale data from Whole Genome Sequencing, followed by comparing cancer and control samples to identify alterations potentially contributing to the development of drug resistance. 2. 2005-2010. The Centre of Postgraduate Medical Education in Warsaw (CMKP), Department of Biochemistry and Molecular Biology; studies in medical biology, supervisor: professor Alicja Nauman, Ph.D; doctoral dissertation title: The Role of UnTranslated Regions (UTRs) in expression of T3-dependent nuclear thyroid hormone receptor 1 (TR 1) in human clear cell renal cell carcinoma (ccRCC). 5-year full-time PhD studies + 2010-2016 cancer research grants at department of Biochemistry and Molecular Biology. 2016 -public defense of doctoral dissertation. 3. 1996-2001. Jagiellonian University (UJ) in Cracow; Faculty of Biochemistry, Biophysics and Biotechnology, Department of Cell Biology (CB), Master's theses: The influence of extracellular calcium ions on contact-mediated acceleration of migration of mouse melanoma B16 cells; 5-years, full-time higher education studies in the field of biology, specialization of Molecular Biology, completed in 2001 with the overall grade: very good and the title of Master of Science (M.sc.). 4. 2001-2004. Cracow University of Technology (PK); Faculty of of Chemical Engineering and Technology, Institute of Organic Chemistry and Technology (ChT); Engineer's theses: Engineering of plasmid-based genetic construct encoding fusion protein: ubiquitin-ubiquitin protease 4, supervisor: prof. Jan Ogonowski and prof. Andrzej Plucienniczak from Institute of Biotechnology and Antibiotics in Warsaw, 4-years higher education studies in chemical technology, specialty of Light organic technology, completed in 2004 with the overall grade: very good and the title of Engineer (Eng.). Fields of Study

Biology: Molecular biology, medical biology, cancer research (in vitro and in vivo animal models), bioinformatics, genomics. Chemistry: chemical engineering, biotechnology of recombinant proteins, synthesis of anticancer drugs, molecular targeting. PhD

Eng.

M.sc.

Post

doc

WORK EXPERIENCE

05/19/2023 – present; DNAi - The Centre of Genetic Information (DNAi), Department of Genetics, EU-US/NYC; Research Scientist, performing AI-powered genetic analyses to recruit cancer patients for appropriate clinical trials; searching in DNA for driving mutations in tumor metastases (paraffin sections of tumor metastases) and/or circulating tumor DNA in blood; navigating and coordinating the efforts of other NGS companies, clinical physicians, searching for targeted therapies (including immunotherapies, nucleic acid-based therapeutics, small molecules) and enrolling patients in clinical trials such as listed on clinicaltrials.gov (NIH). 01/03/2018 – 2023; The State University of New York at Stony Brook, Department of Medicine, Division of Cancer Prevention; position: Postdoctoral Associate (2018-2023) followed by Research Scientist (2023); Testing new anticancer drugs, combination therapy, chemical synthesis of new drug derivatives, molecular action of drugs, bioinformatics, analysis of Whole Genome Sequencing, PharmacoScan, Big data, gene expression microarrays, DNA/RNA databases, in vitro (cell culture) and in vivo studies (mouse xenograft models), Realtime PCR screening methods, cell cultures, fluorescence and confocal microscopy, teaching

(students), mechanistic studies on paclitaxel induced neuropathic pain. 02/18/2014 – 12/31/2017; DNAi - The Centre of Genetic Information, lab leader, geneticist, court expert in genetics and biochemistry, forensic sciences and genetic diagnostics in personalized molecular medicine, performing bioinformatic analyses of genetic data on place (2014-2017) and remotely (2018-2023). Cooperation with academia in translational control in cancer research, 09/01/2005 – 2016; The Centre of Postgraduate Medical Education (CPME), Department of Biochemistry and Molecular Biology headed by professor Alicja Nauman (Centre of New Technologies UW); Medical biology, cell biology, genomics, transcriptomics, proteomics, cancer research, gene expression, translational control in the context of cancer research. Position: PhD student. http://www.cmkp.edu.pl/ . 02/26/2005 – 2016; Laboratory of Molecular Medical Biology of BioTe21, Molecular medicine, RNAi, RNAa, epigenetics, cancer therapy, scientific projects for European Structural Funds - the Integrated Regional Operational Programme

(IROP) and the 7th European Union Frame Programme (FP7); laboratory designer, coordinator of scientific projects, inventor of new genetic diagnostics and innovative biotechnologies aimed at gene therapy and molecular diagnostics. Position: scientific project director. http://www.biote21.com/html/en/index-en.html, http://mtdnatest.com/, http://www.sciencemarket.pl/?lang=en 04/21/2005 – 2008; Nucleagena Ltd, Building project of Laboratory of molecular diagnostics, scientific projects for European Structural Funds, implementation of technologies for medical diagnostics. https://nucleagena.pl/ 03/01/2003 - 25.02.2005; Institute for DNA Research in Warsaw; Molecular biology, Laboratory diagnostics, DNA/RNA analysis, genetic engineering in the context of recombinant proteins; http://ibdna.pl//eng/, 11/01/2002 - 28.02.2003; Nencki Institute of Experimental Biology, Laboratory of Transcription Regulation, headed by professor Bozena Kaminska-Kaczmarek, Molecular biology, cloning, gene expression in the context of transcriptional control; http://en.nencki.gov.pl/, http://en.nencki.gov.pl/laboratory-of-molecular-neurobiology, 04/01/2002 - 30.10.2002; Institute for DNA Research in Warsaw. Molecular biology, DNA analysis, paternity testing, medical genetic diagnostics http://ibdna.pl//eng/ 07/01/2001 - 30.03.2002; Institute of Farmacology of Polish Academy of Science in Cracow; Department of Molecular Neuropharmacology headed by professor Ryszard Przewlocki. Molecular biology, cell culture, gene expression profiling in the context of drug abuse, http://www.if-pan.krakow.pl/ifpan.

TECHNICAL EXPERIENCE

In my scientific work I have taken advantage of the most advanced research tools and high-resolution apparatus enable of performing the synthesis and analysis at genome, transcriptome and proteome levels: o Whole genome sequencing, PharmacoScan, RNAseq, gene expression microarrays, bioinformatics o Chemical synthesis of new anticancer drugs and their derivatives, o In vitro cell clultures, cell cultures in three-dimensional collagen matrix and in vivo mice xenograft models, o Cell transfection (PEI, liposome-, retro-, lentivirus-based transfection), o DNA Sequencing (using 310, 3100, 3130xl Genetic Analyzers, Applied Bioscience), o ABI 3900 High Throughput Nucleic Acid Synthesizer (AppliedBiosystems), synthesis of n.a. derivatives, o STR, SNaPshot, SNaPlex multiplex genotyping technologies (ThermoFisher), projects of multiplex PCRs, o Bioinformatic tools, genome and transcriptome analysis (NCBI tools, RNAstructure, Oligo…), Excel-based programming (SNPtester, STRtester, dGEnhancer), generating and analysis of databases, o Microarray technology (human, mouse whole genome, SNP, CGH, miRNA; Agilent, Affymetrix, Ambion), o PCR and Quantitative Real-Time PCR (Roche, BioRad, Rotorgene, Stratagene), o Mass and gas spectrometry (MS, Agilent), spectrophotometry (Nanodrop) o High-Performance Liquid Chromatograph ( HPLC, Hitachi Transgenomic WAVE DNA Analysis System), analytical chemistry and biochemistry, o In vitro coupled transcription/translation System, luminometry (Roche), o Engineering of recombinant proteins in bacterial and cellular systems, o Targeting cis-acting elements recognized by trans-acting factors

(siRNA, microRNA, lncRNA, saRNA and translation-enhancing dGoligos), o Laser Microdissection & Optical Tweezers PALM MicroBeam (Zeiss), single cell genome amplification, o Fluorescence microscopy (Nicon, Olympus).

o Since 2001, I am an expert witness working for courts, prosecutors, police, laboratories, detective agencies and private persons.

o Over the last 2 decades I have issued several thousands opinions in genetics (genetic identification, paternity testing, genealogy), biochemistry, narcotics and dactyloscopy. Interested in: translational control in cancer, oncolytic viruses, transfection with exosomes, circular RNA, long non coding RNA, CRISPR, synthetic biology.

REFEREED PUBLICATIONS http://orcid.org/0000-0001-9391-8846

[1] Master A, Huang L, Tsirukis VE, Loiacono A, Khawja A, Takmil C, Tsioulias A, Honkanen R, Rigas B . Transcriptomic landscape of the Dorsal Root Ganglia (DRG) and the Sciatic Nerve (SN) in a mouse model of Paclitaxel-Induced Peripheral Neuropathy (PIPN). PLoS One in preparation. Transcriptome datasets published on Dec 08 2023, NCBI GEO acc. no.: GSE249643. link

[2] Master A, Huang W, Huang L, Honkanen R, Rigas B. An Improved Ocular Impression Cytology Method: Quantitative Cell Transfer to Microscope Slides Using a Novel Polymer. Curr Eye Res. 2022 Jan;47(1):41-50. link

[3] Master A, Huang W, Huang L, Li W, Saglam S, Honkanen R, Rigas B. Simplified ex-vivo drug evaluation in ocular surface cells: Culture on cellulose filters of cells obtained by impression cytology. Exp Eye Res. 2021 Dec;213:108827. link

[4] Huang W, Huang L, Li W, Saglam MS, Tourmouzis K, Goldstein SM, Master A, Honkanen R, Rigas B. Once-Daily Topical Phosphosulindac Is Efficacious in the Treatment of Dry Eye Disease: Studies in Rabbit Models of Its Main Clinical Subtypes. J Ocul Pharmacol Ther. 2021 Dec 29. link

[5] Master A, Kontzias A, Huang L, Huang W, Tsioulias A, Zarabi S, Wolek M, Wollocko BM, Honkanen R, Rigas B. The transcriptome of rabbit conjunctiva in dry eye disease: Large-scale changes and similarity to the human dry eye. PLoS One. 2021 Jul 29;16(7):e0254036. link

[6] Koronowicz AA, Master A, Banks P, Piasna-Słupecka E, Domagała D, Drozdowska M, Leszczyńska T. PPAR Receptors Expressed from Vectors Containing CMV Promoter Can Enhance Self-Transcription in the Presence of Fatty Acids from CLA-Enriched Egg Yolks-A Novel Method for Studies of PPAR Ligands. Nutr Cancer. 2020;72(5):892-902. link

[7] Aneta A. Koronowicz, Paula Banks, Adam Master, et al., “Fatty Acids of CLA-Enriched Egg Yolks Can Induce Transcriptional Activation of Peroxisome Proliferator-Activated Receptors in MCF-7 Breast Cancer Cells,” PPAR Research, vol. 2017, Article ID 2865283, 12 pages, 2017. PDF-link.

[8] Master A, Wójcicka A, Giżewska K, Popławski P, Williams GR, Nauman A. A Novel Method for Gene-Specific Enhancement of Protein Translation by Targeting 5’UTRs of Selected Tumor Suppressors. PLoS ONE. 2016 May 12; 11(5): e0155359. PDF- link.

[9] Koronowicz AA, Banks P, Domagała D, Master A, Leszczyńska T, Piasna E, Marynowska M, Laidler P. Fatty acid extract from CLA-enriched egg yolks can mediate transcriptome reprogramming of MCF-7 cancer cells to prevent their growth and proliferation. Genes Nutri. 2016;11:22. PDF- link.

[10] Koronowicz AA, Banks P, Szymczyk B, Leszczyńska T, Master A, Piasna E, Szczepański W, Domagała D, Kopeć A, Piątkowska E, Laidler P. Dietary conjugated linoleic acid affects blood parameters, liver morphology and expression of selected hepatic genes in laying hens. Br Poult Sci. 2016 Jun 8.PDF-link.

[11] Koronowicz AA, Kopeć A, Master A, Smoleń S, Piątkowska E, Bieżanowska-Kopeć R, Ledwożyw-Smoleń I, Skoczylas Ł, Rakoczy R, Leszczyńska T, Kapusta-Duch J, Pysz M. Transcriptome Profiling of Caco-2 Cancer Cell Line following Treatment with Extracts from Iodine- Biofortified Lettuce (Lactuca sativa L.). PLoS One. 2016 Jan 22;11(1):e0147336. PDF- link.

[12] Wojcicka A, Piekielko-Witkowska A, Kedzierska H, Rybicka B,Poplawski P, Boguslawska J, Master A, Nauman A. Epigenetic regulation of thyroid hormone receptor Betain renal cancer. PLoS One. 2014 May 21;9(5):e97624. doi: 10.1371/ journal.pone.0097624. eCollection 2014. PubMed PMID: 24849932. Click the link.

[13] Master A, Nauman A. THRB (Thyroid Hormone Receptor, Beta). Atlas Genet Cytogenet Oncol Haematol. 2014; 18(6). This article was published as online updated version in November 2013 and is available under the following links: Article–HTML; Article–PDF; AGCOH no. 06, 2014.

[14] Master A, Nauman A. Molecular mechanisms of protein biosynthesis initiation - biochemical and biomedical implications of a new model of translation enhanced by the RNA hypoxia response element (rHRE) [Molekularne mechanizmy inicjacji biosyntezy białek - biochemiczne i biomedyczne implikacje nowego modelu translacji wzmacnianej przez element RNA odpowiedzi na hipoksję (rHRE)]. Postepy Biochem. 2014;60(1):39-54. Review. Click the link. The Polish Biochemical Society has granted this work with Bolesław Skarżyński Award for the best article published in a quarterly journal “Postępy Biochemii” published in year 2014.

[15] Master A, Nauman A. Gene expression regulation by long naturally occurring antisense transcripts [Regulacja ekspresji genów przez długie naturalnie występujące antysensowne transkrypty]. Post. Biol. Kom. 2014;41(1):3-28. Review. Click the link.

[16] Master A, Nauman A. Genomic context and expression regulation of nuclear thyroid hormone receptors by long naturally occurring antisense transcripts [Genomowy kontekst i regulacja ekspresji receptorów hormonu tarczycy przez długie naturalnie występujące antysensowne transkrypty]. Post. Biol. Kom. 2014; 41(1):29-58. Review. Click the link.

[17] Boguslawska J, Wojcicka A, Piekielko-Witkowska A, Master A, Nauman A. MiR-224 targets the 3'UTR of type 1 5'-iodothyronine deiodinase possibly contributing to tissue hypothyroidism in renal cancer. PLoS One. 2011;6(9):e24541. doi: 10.1371/journal.pone.0024541. Epub 2011 Sep 2. Click the link.

[18] Master A, Wójcicka A, Piekiełko-Witkowska A, Bogusławska J, Popławski P, Tański Z, Darras VM, Williams GR, Nauman A. Untranslated regions of Thyroid hormone receptor beta 1 mRNA are impaired in human clear cell renal cell carcinoma. Biochim Biophys Acta. 2010 Nov;1802(11):995-1005. doi: 10.1016/j.bbadis.2010.07.025. Epub 2010 Aug 3. Html-link; PDF-link.

[19] Piekielko-Witkowska A, Master A, Wojcicka A, Boguslawska J, Brozda I, Tanski Z, Nauman A. Disturbed expression of type 1 iodothyronine deiodinase splice variants in human renal cancer. Thyroid. 2009 Oct;19(10):1105-13. doi: 10.1089/thy.2008.0284. PubMed PMID: 19534619. Click link.

[20] Nauman A, Piekiełko – Witkowska A, Turowska O, Popławski P, Master A, Tański Z, Lampkowska J, Wójcicka A, Brózda I, Puzianowska – Kuźnicka M. Disturbances of thyroid hormone signal pathway – in clear cell renal cell carcinoma [Zaburzenia szlaków sygnałowych hormonu tarczycy -trijodotyroniny - w raku nerki typu jasnokomórkowego]. Postepy nauk medycznych. 2008, 5: 268-278. Html-link.

[21] Wisniewski P, Master A, Kaminska B. Cloning and purification of functionally active Fas ligand interfering protein (FIP) expressed in Escherichia coli. Acta Biochim Pol. 2008;55(1):51-6. Epub 2008 Jan 18. Click the link.

[22] Wesolowska A, Kwiatkowska A, Slomnicki L, Dembinski M, Master A, Sliwa M, Franciszkiewicz K, Chouaib S, Kaminska B. Microglia-derived TGF-beta as an important regulator of glioblastoma invasion—an inhibition of TGF-beta-dependent effects by shRNA against human TGF- beta type II receptor. Oncogene. 2008 Feb 7;27(7):918-30. Epub 2007 Aug 6. Click the link.

[23] Nauman A, Turowska O, Poplawski P, Master A, Tanski Z, Puzianowska-Kuznicka M. Elevated cyclin E level in human clear cell renal cell carcinoma: possible causes and consequences. Acta Biochim Pol. 2007;54(3):595-602. Epub 2007 Aug 28. Click the link.

[24] Turowska O, Nauman A, Pietrzak M, Popławski P, Master A, Nygard M, Bondesson M, Tanski Z, Puzianowska-Kuznicka M. Overexpression of E2F1 in clear cell renal cell carcinoma: a potential impact of erroneous regulation by thyroid hormone nuclear receptors. Thyroid. 2007 Nov;17(11):1039-48. PubMed PMID: 17910524. Click the link.

[25] Switaj K, Master A, Borkowski PK, Skrzypczak M, Wojciechowicz J, Zaborowski P., Association of ocular toxoplasmosis with type I Toxoplasma gondii strains: direct genotyping from peripheral blood samples, J Clin Microbiol. 2006 Nov;44(11):4262-4. Click the link.

[26] Switaj K, Master A, Skrzypczak M, Zaborowski P. Recent trends in molecular diagnostics for Toxoplasma gondii infections. Clin Microbiol Infect. 2005 Mar;11(3):170-6. Review. link.

[27] Madeja Z, Master A, Michalik M, Sroka J. Contact-mediated acceleration of migration of melanoma B16 cells depends on extracellular calcium ions. Folia Biol. 2001;49(3-4):113-24. Click the link. SELECTED ABSTRACTS

[1] Adam Master 1, Anna Wojcicka 2,3, Alicja Nauman 2 . Gene-specific enhancement of protein synthesis by targeting 5’UTRs -a novel oligonucleotide-based strategy for translational control of selected tumor suppressors. 3rd International Conference and Expo on Drug Discovery & Designing October 02-03, 2017, Vienna, Austria. Theme: The developmental strategies and Various new advances in Drug Discovery and Designing through a decade in Europe. 1 DNAi - The Center of Genetic Information, Laboratory of Molecular Medical Biology, ul. Baltycka 3, 31-214 Krakow, Poland; 2 Laboratory of Human Cancer Genetics, Centre of New Technologies, University of Warsaw, Banacha 2c, 02-089 Warsaw, Poland; 3 Genomic Medicine, Medical University of Warsaw, Zwirki i Wigury 61, 02-091 Warsaw, Poland.

[2] Master A1, Wojcicka A2, Nauman A1. The 5’UTR-dependent enhancement of protein translation efficiency triggered by self-transfecting 3’-aminoallyl- containing oligonucleotides (aa-dGoligos) targeting a pool of strongly folded transcript variants of the THRB suppressor gene. Molecular Therapy Volume 22, Supplement 1, May 2014 (Nature Publishing Group). The 17th Annual Meeting of the American Society of Gene & Cell Therapy, May 21-24, 2014 Washington, DC, USA. Session: Gene Targeting and Gene Correction I. 1The Centre of Postgraduate Medical Education, Department of Biochemistry and Molecular Biology, ul. Marymoncka 99/103, 01-813 Warsaw, Poland. 2Genomic Medicine, Depart. of General, Transplant and Liver Surgery, Medical University of Warsaw, ul. Żwirki i Wigury 61, 02-091 Warsaw, Poland. To see click the poster-link or abstract-link.

[3] Koronowicz A1, Master A2, Sikora E1, Leszczynska T1, Dulinska-Litewka J3. The influence of fatty acids of egg yolks enriched with CLA on mRNA expression of TP53, CASP3 i MYC genes. [Wpływ mieszaniny kwasów tłuszczowych z żółtka wzbogaconego w CLA na ekspresję mRNA genów: TP53, CASP3 i MYC]. 1) University of Agriculture in Cracow, Faculty of Food Technology, Poland, 2) Centre of Postgraduate Medical Education, Department of Biochemistry and Molecular Biology, Warsaw, Poland, 3) Jagiellonian University, Collegium Medicum, Department of Medical Biochemistry. XXIII Polish Bromatological Symposium Bromatology for XXI Century Society”. Cracow 10-12th of September 2014r.

[4] Domagala D1, Koronowicz A1, Master A2, Dulinska T4, Leszczynska T1. Antagonistic effect of CLA isomers on expression of selected cell cycle genes in breast Department of Biochemistry and Molecular Biology, Warsaw, Poland; 3) Department of Medical Biochemistry, Jagiellonian University, Collegium Medicum, Krakow, Poland. Abstract (1835) accepted for presentation at FEBS-EMBO congress, Paris, France from 30 August - 4 September 2014.

[5] Koronowicz Aa, Master Ab, Chwastek Ja, Szymczyk Bc Leszczynska Ta. The influence of fatty acids of egg yolks enriched with CLA on activation of PPAR receptors in prostate cancer cells [Wpływ kwasów tłuszczowych z żółtek jaj wzbogaconych w CLA na aktywację receptorów PPAR w komórkach rakowych gruczołu krokowego]. XLI session of Food Science Committee of Polish Academy of Science, Cracow, 2-3th of July 2013. Session: Modern techniques and technologies in food processing and food conservation. a) University of Agriculture in Cracow, Faculty of Food Technology, Poland, b) Centre of Postgraduate Medical Education, Department of Biochemistry and Molecular Biology, Warsaw, Poland, c) Faculty of Animal Feeding and Technology, University of Agriculture in Cracow, Poland, and National Research Institute of Animal Production, Department of Animal Nutrition and Feed Science, Poland.

[6] Boguslawska J, Piekielko-Witkowska A, Wojcicka A, Master A, Nauman A, Mir-224 contributes to tissue hypothyroidism in renal cancer, targeting the 3'UTR of type 1 5 -iodothyronine deiodinase, 35th Meeting of the European Thyroid Association, 10-14.09.2011 Krakow, European Thyroid Journal, Launching Issue, p. 78.

[7] Piekiełko-Witkowska A, Master A, Wojcicka A, Bogusławska J, Wiszomirska H, Tanski Z, Darras VM, Williams GR, Nauman A. Zaburzenia ekspresji receptora hormonu tarczycy TRβ1 w raku nerki człowieka”. 2 Zjazd Polskiego Towarzystwa Tyreologicznego (2d Meeting of Polish Thyroid Association), 20- 22.05.2010 Zakopane, Endokrynologia Polska tom 61 nr 5, 2010, str 578.

[8] Bogusławska J, Master A, Piekiełko-Witkowska A, Wójcicka A, Popławski P, Tański Z, Nauman A. Rola mikroRNA w zaburzeniach ekspresji jodotyroninowej dejodynazy typu I w raku nerki typu jasnokomórkowego (ccRCC). 2 Zjazd Polskiego Towarzystwa Tyreologicznego (2d Meeting of Polish Thyroid Association), 20-22.05.2010 Zakopane, Endokrynologia Polska tom 61 nr 5, 2010, str 578.

[9] Boguslawska J, Master A, Wojcicka A, Popławski P, Piekielko-Witkowska A, Nauman A. The Role of miRNA in reduction of type I 5’-iodothyronine deiodinase expression (D1) in renal clear cell carcinoma (ccRCC). 12-th European Congress of Encocrinology 24-29.04.2010 Prague, Czech, Endocrine Abstracts 2010, 22.

[10] Wójcicka A, Piekiełko-Witkowska A, Popławski P, Master A, Lampkowska J, Brózda J, Tański Z, Nauman A. Potencjalna rola metylacji w wyciszaniu ekspresji genu receptora jądrowego hormonu tarczycy TRbeta w raku nerki typu jasnokomórkowego. 1 Zjazd Polskiego Towarzystwa Tyreologicznego, 26- 28.03.2009 Szczecin, Endokrynologia Polska, 2009; 60; supl.A; 3-4.

[11] Bogusławska J, Master A, Piekiełko-Witkowska A, Wójcicka A, Popławski A, Brózda I, Tański Z, Nauman A. Rola 3’UTR I mikroRNA w zaburzeniach ekspresji jodotyroninowej dejodynazy typu I w raku nerki typu jasnokomórkowego (ccRCC). 1 Zjazd Polskiego Towarzystwa Tyreologicznego, 26-28.03.2009 Szczecin, Endokrynologia Polska, 2009; 60; supl.A; 3-4.

[12] Boguslawska J, Master A, Piekielko-Witkowska A, Wojcicka A, Brozda I, Poplawski P. Macke-Nauman A. Reduction of type I 5'-iodothyronine deiodinase activity and mRNA level in renal clear cell carcinoma (ccRCC): potential role of 3' UTR and miRNA. BES 15-19.03.2009, Harrogate, Great Britain.

[13] Boguslawska J, Master A, Piekielko-Witkowska A, Wojcicka A, Poplawski P, Nauman A. The micro RNA targets the 3’UTR of type I 5’-iodothyronine deiodinase resulting in the reduction of D1 mRNA level in renal clear cell carcinoma (ccRCC). EMBO workshop Messenger RNA 3’ ends & gene expression, 16-20.09.2009 Oxford, Great Britain.

[14] Master A1, Wojcicka A1, Poplawski P1, Piekielko-Witkowska A1, Boguslawska J1, Brozda I1, Wiliams GR2 and Nauman A1. Short oligodeoxynucleotides

(dgoligos) targeting of the THRB gene 5'-UTR markedly increase the efficiency of TRβ protein translation - a therapeutic potential of sense/antisense based dGoligos as Gibb's free energy modulators of 5'-utr stem-loop structure. 05-09.09.2009. Lisbon, European Thyroid Association. Acta Medica Portugesa, 2009; 22; 4(11); s.13. The Young Investigators’ Prize. 1The Centre of Postgraduate Medical Education, Department of Biochemistry and Molecular Biology, ul. Marymoncka 99/103, 01-813 Warsaw, Poland. 2Molecular Endocrinology Group, Imperial College London, MRC Clinical Sciences Centre, Hammersmith Hospital, Du Cane Road, London, W12 0NN. Click the link.

[15] Master A1, Piekielko-Witkowska A1, Poplawski P1, Lampkowska J1,Wojcicka A1, Brozda I1, Tanski Z2 Williams GR3,Alicja Nauman1. Aberrant expression of human thyroid hormone receptor b1 protein may be regulated by alternative untranslated

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