CURRICULUM VITAE

Dr. Ruchi Pathania

Postdoctoral Scholar

University of California San Diego (UCSD), USA

Email:ad3q9j@r.postjobfree.com, ad3q9j@r.postjobfree.com Contact Number: +1-646-***-****

Linkedin profile link: https://www.linkedin.com/in/ruchi-pathania-30605082/ Summary

I am experienced Scholar in Biotechnology and Molecular Biology having skills in Synthetic Biology, Strain Engineering, Microbiology, Labeled Metabolomics, Vector cloning and genome editing. I have worked with E.coli, Yeast and mostly with photosynthetic microorganisms (especially Cyanobacteria) since past 8 years (6 years in PhD and 1 year 8 months in Postdoc).

In my postdoc at UCSD, I have engineered marine cyanobacterium for producing high-value compounds for biotechnological applications and contributing in other projects running in the lab for microalgae promoter library preparation.

During my PhD, I learned skills for Molecular Biology, Metabolomics, Fluxomics, Strain development, Optimization under different environmental conditions, Culturing and transformation of cyanobacteria.

I would like to emphasize that I can design, perform and analyze experiments individually as well as can work well in collaborations also. I am an enthusiastic fast learner, creative, logic based problem solver and have ability to do research, create and deliver high quality content. I always look for a new challenge in science-based research.

I have trained/training master students and interns during my research journey. I am also volunteering as a Reviewer in Journals: Bioenergy Research, MDPI (applied microbiology, phycology), Frontiers in Environmental Microbiology, Microbial cell factories, Journal of Engineering Research and Sciences (JENRS) etc.

Skills

Molecular Biology Standard molecular biology techniques including: PCRs (different types), Gene cloning,

Expression vector design,

Transformation of E. coli,

Cyanobacteria and microalgae

DNA and RNA extraction

RT-qPCR for transcript expression

Protein extraction and estimation,

SDS-PAGE

Western Blotting and 2-D gel electrophoresis

Strain engineering in Cyanobacteria, Microalgae.

Experience in culturing photoautotrophs, E.coli and yeast Optimization under different environmental conditions Handling with 3L bioreactor and ponds at the field station for biomass production

Adaptation of yeast for salt stress and running strain in 500 ml bioreactors for ethanol production

Analytical Biochemical characterization (Bioassays) Metabolomics (Metabolites extraction

and quantification)

Data Analysis of:

High-performance liquid chromatography (HPLC)

Gas Chromatography-Mass Spectrometry (GC-MS)

Systems Biology Fluxomics: Metabolic Flux Analysis (MFA) using 13C labeled tracer molecules and computation analysis. Genome-scale metabolic model construction

(GSMMs)

Professional experience

Postdoctoral Scholar (July 2022 - Present)

University of California, San Diego, California, USA Molecular Biology Department

Ph.D. (Life Science) (August 2015 - April 2022)

ICGEB, New Delhi, India

Visiting Researcher (Jan 2020 - Nov 2020)

Oxford Brookes University, United Kingdom

Department of Biological and Medical Sciences

Educational qualifications

Ph.D. (Life Science) “Evaluating the potential of an indigenous marine

(2015-2022) cyanobacterium as a feedstock for biofuel production through medium and genetic engineering and its metabolomics analysis”

Supervisor: Dr. Shireesh Srivastava

Systems Biology for Biofuel Group,

ICGEB, New Delhi, India

Visiting Researcher Supervisor: Dr.Mark Poolman

(Jan 2020-Nov 2020) Oxford Brookes University, United Kingdom Masters of Science Biochemistry

(2012-2014) Panjab University, Chandigarh, India

Bachelors of Science Biochemistry

(2009-2012) Panjab University, Chandigarh, India

List of Publications

Pathania R, Srivastava S (2020). Synechococcus elongatus BDU 130192, an Attractive Cyanobacterium for Feedstock Applications: Response to Culture Conditions. Bioenerg Res. https://doi.org/10.1007/s12155-020-10207-7.

Pathania R, Ahmad A, Srivastava S (2020). Biochemical Characteristics and a Genome- Scale Metabolic Model of an Indian Euryhaline Cyanobacterium with High Polyglucan Content. Metabolites 10:177. https://doi.org/10.3390/metabo10050177.

Pathania R, Ahmad A, Srivastava S (2017). Draft Genome Sequence of an Indian Marine Cyanobacterial Strain with Fast Growth and High Polyglucan Content. Genome Announc 5: https://doi.org/10.1128/genomeA.01334-17.

Pathania R, Srivastava A, Srivastava S, Shukla P (2021). Metabolic systems biology and multi-omics of cyanobacteria: perspectives and future directions. Bioresource Technology 126007. https://doi.org/10.1016/j.biortech.2021.126007.

Ahmad M, Pathania R, Chowdhury A, et al (2021). Salt-stress adaptation of yeast as a simple method to improve high-gravity fermentation in an industrial medium. Appl Microbiol Biotechnol. https://doi.org/10.1007/s00253-021-11566-7.

Pallavi Saxena, Pritikrishna Majhi, Ruchi Pathania, Sarieh Tarigholizadeh, Harish and Tatiana Minkina (2023). Advanced value-added bioproducts from microalgae. (Book chapter in Algal Biotechnology) https://doi.org/10.2166/9781789063547_0183.

Gupta A, Kang K, Pathania R, Saxton L, Saucedo B, Malik A, Torres-Tiji Y, Diaz CJ, Dutra Molino JV and Mayfield SP (2024). Harnessing genetic engineering to drive economic bioproduct production in algae. Front. Bioeng. Biotechnol. 12:1350722. https://doi.org/10.3389/fbioe.2024.1350722.

Ruchi Pathania, Preeti Rai, Namrata Bhagat, Srabani Kar, Shireesh Srivastava and Pratyoosh Shukla. Molecular tools for reprogramming stress tolerance in cyanobacteria

(under review).

Ahmad Ahmad, Kashif M. Shaikh, Ruchi Pathania, Shireesh Srivastava and Pannaga Pavan Jutur. A genome-scale metabolic model of an oleaginous trebouxiophycean microalga Parachlorella kessleri for a systems-level analysis of its metabolism (under review).

Awards and Honors

microMBA course 2023 (for 2 months) from UCSD extended studies.

Science and Engineering Research Board (SERB)-Overseas Visiting Doctoral Fellowship (OVDF) Fellow (2018) for visiting Oxford Brookes University for 9 months.

Recipient of International travel grant from Council of Scientific and Industrial Research

(CSIR) for attending Course “Bioinformatics: computer methods in molecular and systems biology” at ICGEB, Trieste, Italy from 24-28 June, 2019.

Poster presentation in 9th International Conference on Algal Biomass, Biofuels and Bioproducts (AlgalBBB 2019) at Boulder (CO, USA) from 17-19 June 2019.

CSIR-NET-JRF, Dec 2014 (Life Sciences) with All India Rank – 13 (National eligibility test for Ph.D. Fellowship).

Qualified GATE 2015 (Life Sciences).

Panjab University, Chandigarh, India Ph.D. Entrance Exam 2014- 3rd Position.

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