Patrick Funk Patrick Funk

***** ** **** **

Kansas City, MO 64153

913-***-****

ad33uz@r.postjobfree.com

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Skills Over 20 years of experience in Research and Development of animal vaccines, with a wide range of skills including manufacturing and Quality Control. Experience as a business owner and property management. ㅡ

Experience Merck Manufacturing / Quality Control Associate FEBRUARY 2023 - PRESENT, DeSoto, KS

● Safety testing in mice and guinea pigs

● Potency testing and toxin neutralizing tests in rabbits and mice

● SAP: test planning and data entry

Merck Manufacturing / Operations Engineer

SEPTEMBER 2015 - FEBRUARY 2023, DeSoto, KS

● SAP: Goods Receipt/Goods Issue, Maintenance Orders

● Safety Inspections, Implementation, 3-D Printing of Safety Devices

● Cell Culture Fermentations

● Large Scale Chromatography of Monoclonal Antibodies

● Bio-Safety Assessment and Inspections

Merck Manufacturing / Research and Development Scientist AUGUST 2008 - SEPTEMBER 2015, DeSoto, KS

● Biological assays for serum neutralization of virus or bacteria

● Vaccine evaluation, production, antigen processing, formulations

● PCR/Recombinant DNA technique (Identity testing, primer design, diagnostics)

● Reverse Transcriptase Assay

● Tissue culture and growth optimization of multiple cell lines Intervet / Research and Development Scientist

AUGUST 2001 - AUGUST 2008, DeSoto, KS

● Hybridoma production, screening and selection of monoclonal antibodies using a database, production of mouse ascites fluid

● Purification and characterization of viral and virus-like-particle proteins

● Production-scale hollow fiber and ion-exchange chromatography purification using AKTA Unicorn software

● Animal testing in horses, hamsters, guinea pigs in research facility or field locations, experience working in Biosafety Level 3 conditions

● HPLC Stability monitoring of vaccine antigens

Intervet / Research and Development Lab Technician JUNE 1996 - AUGUST 2001, Millsboro, DE

● Bacterial culture and large scale fermentation

● Vaccine Evaluation, Production, Antigen Processing, Formulations

● ELISA Reagents and Test Development

● PCR/Recombinant DNA technique (plasmid expressed protein, DNA purification, sequence analysis)

● Writing of new SOP’s and animal experiments for R&D and Quality Control

● Purification and HRP of biotin labeling of antibodies for R&D and Quality Control

● Animal testing in dogs and mice for USDA experiments

● Collection and maintenance of Ixodes scapularis ticks, development of a novel exchange method using ticks

● Production problem solving and development of a

manufacturing procedure to reduce endotoxin

KSU Veterinary Hospital / Research Assistant

JUNE 1991 - DECEMBER 1995, Manhattan, KS

● Protein chemistry, purification

● Biological assays for toxin activity

● Recombinant DNA technique (RFLP, transposons, cloning, electroporation, restriction enzyme digestion, and ligation)

● SDS-PAGE/Western Blot

● Agarose gel electrophoresis/Southern Blot

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Education Wilmington College / Master’s of Business Administration AUGUST 1998 - DECEMBER 2000, Georgetown, DE

Kansas State University / Bachelor of Science in Microbiology JUNE 1991 - DECEMBER 1995, Manhattan, KS

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Awards

● Recipient, Phi Zeta Food Animal Production Research Award

(1994)

● Student, Immunological Methods Course at Biotechnology Training Institute (1997)

● Registered Laboratory Animal Technician (RLAT) (1997)

● Stability Testing of Proteins, Peptides, and other Biomolecules Seminar (2007)

● HPLC Operator Training at Agilent Inc. (2012)

● ABSA Biosafety Training Seminar at CSU (2021)

Publications and Patents:

● Funk, P.G., et al. (1996) "Identification and Partial Characterization of an Actinomyces pyogenes Hemolysin" Veterinary Microbiology 50:129-142.

● Novel process to chemically separate endotoxin from a recombinant protein of the same molecular weight on a production scale.

● Novel process for ultracentrifugation of virus and western blotting of PEDV field samples from tech services veterinarians.

● Patent: Growth of Ehrlichia canis in multiple cell lines (Fibroblasts vs. Monocytes).

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