CURRICULUM VITAE

Sherry Shaobing Zhang, M.D, Ph.D

Irvine, CA92612

US citizen, Fully vaccinated.

Cell: 1-814-***-****

Email: ad2t3y@r.postjobfree.com

Education:

Ph.D. Cell, Molecular and Developmental Biology, Oulu University, Finland M.D. Fujian Medical School, China

Highlights:

Expertise in NGS (three days passed 4 sequencing runs) Expertise on extraction of DNA/RNA from tissue, blood, and cell Experiences in therapeutic antibodies development

Good at designing experiment, troubleshooting, and finding the solution Expertise on cell and molecular biology, immunology, and Oncology Expertise on gene targeting & editing at cell, Zebrafish, and mouse system Experience at working animal model (mouse)

Expertise in large DNA fragment (genomic DNA more than 150kb) cloning Four years researcher training at Harvard University Strong medical knowledge with MD degree and six years surgical doctor practice Named as one of the best employers by Boss in his 40 years employee career Professional Activities

Dec 2021-Dec 2022: Scientist, molecular biology in Clinical Next-Generation Sequencing Division/Thermo Fisher Scientific

Screen cancer patients' gene mutation using NGS technology and analytical validation of NGS-based IVD products (Ion Torrent platform and Genexus automation platform)

Strong technical knowledge and expertise in molecular biology methods. Hand- on experiences in IVD product development using next generation sequence technique (NGS): DNA & RNA extractions (always have good quantity), cDNA synthesis, PCR &qRT-PCR (20 years of experience), next-generation library prep, automated instruments for templating, and DNA/RNA sequencing (passed 4 sequencing runs in three days)

be very proficient to automated Genexus integrated sequencer instruments that scale up 32 samples/sequencing run.

Passed all sequencing runs independently in a project two months earlier than FDA deadline

Familiar with basic bioinformatic tools for NGS analysis, designing, conducting and troubleshooting of experiments; well-interpret results and draw conclusions. Be able to design future experiment plans, protocols, and reports writing. Prepare and presents scientific data and reports in internal and external meetings. Hard worker and able to multitask and maintain several projects at one time. Effectively troubleshooted a problematic reagent issue and provide an easy and universal way to identify any reagent issues using R&D thinking way Thermo fisher scientific proficient NGS operator (Ion Torrent and Genexus). Finished more than 100 trainings (SOPs, GDP, GLP, PPE…) at Themo Fisher Scientific. Understanding FDA guidance and CLIA/CAP regulated setting. Sep 2006- Dec 2021: Senior Medical consultant Vicare International, USA, Los Angeles Drug developing, marketing and quality control

Jan 2019 – Oct 2020: Senior scientist in Ephos bioscience Inc/VasGene Therapeutic Inc, Los Angeles

Development of therapeutic monoclonal antibodies for the treatment of tumor using the following techniques in R&D department

Immunology & Protein biology:

Hand-on experience in production of mAB using hybridoma cell technique

(hybridoma cell fusion, screening, RT-PCR to sequence antibody sequence from hybridoma cells; generate chimeric antibodies; expression proteins (antibodies HC

&LC) in mammalian cell line (ExpiCHO Expression system); Titer and characterize the antibodies by using qualitative and quantitative immunoassays including Elisa, Westernblot, Coomassie blue, IHC and pull-down immunoprecipitation methods; purify antibody using standard chromatography methods.

Cloned and expressed recombinant protein constructs (whole, truncated and fusion protein); transiently transfected into mammalian cell lines (HEK293 & CHO); stable cell line generation (antibiotic selection, stable pool, production of single cell clonies using limiting dilution method, cell banking and expansion); purification and characterization of protein using chromatography, Elisa, Westernblot, IHC and Co- IP methods. Expression and purification of protein in E.Coli system. Cell culture: media preparation, culture and subculturing adherent cells in different sizes of plates (96, 48, 24, 12, 6 wells and 10cm) and different sizes of flasks, and suspension cells (shaking flasks); freezing cells, thawing frozen cells, cell counting Molecular biology: hand-on experiences in primers design, PCR, RT-PCR and QPCR; extraction and purification of plasmid DNA (mini, maxi, Mega and Giga preps), plasmid cloning (enzyme digestion, PCR amplification, TOPO-cloning ect), DNA purification from agarose gel, DNA sequencing and comparison; mutagenesis; transformation of DNA constructs into E.Coli competent cell; total RNA purification from cells or tissue, RNA probe synthesis and working RNAase free environment; cDNA synthesis (Novagen Ig-Primer sets or Biolab DNA amplification &PCR). SiRNA technique (RNAi)

Data analysis: familiar to Microsoft office (word document, excel, Powerpoint), Photoshop.

Effectively to present data regularly in internal meeting and external setting. Strong interpersonal social skill and hard working Maintain a laboratory electronic and hard copy notebook according to GDP Requirement. Optimize the protocol accordingly.

Aug 2011- Nov 2018: Part-time scientist in Penn State University/Eberly college of science Molecular biology: extraction and purification of plasmid DNA, genomic DNA and total RNA from cells and tissue; plasmid cloning; DNA sequencing primers design, PCR, RT-PCR and QPCR

Cell biology: media preparation, subculturing adherent cells and suspension cells, freezing cells, thawing frozen cells, cell counting, transfection and selection, western blot (SDS-PAGE); IHC, CoIP; Elisa, recombinant protein expression, purification, and characterization Mouse husbandry (crossing and breeding); genotyping (numerous and daily PCR and PCR primers design)

Jul 2006- Jul 2011: Senior scientist, VasGene therapeutics Inc & USC/Norris cancer center/ Department of Hematology

1. Gene editing technique: generated and analyzed two conditional knock-out mice lines (Cre/LoxP) and two transgenic mice to study oncological genes EphB4 and Tetraspanin 18 function in angiogenesis and tumor development 2. Screening of cancer genes expression level at various human tumor tissues using microarray, qPCR and immunoassay techniques

3. Immunology & Protein biology: Expression and purification of T18 protein in mammalian cell system, generate T18 polyclonal antibody and titer Rabbit serum by using qualitative and quantitative immunoassays including Elisa, pull-down assay, Westernblot, IHC and ICC.

4. Generation of transgenic and knock out mice: design and construction of transgenic and knock out constructs; genomic DNA isolation, molecular cloning using Bac clones as template; ES cell screening (PCR and southern blot); mouse husbandry (crossing and breeding); genotyping (numerous and daily PCR and southern blot); RT-PCR, QRT-PCR and Microarry; extensive PCR primers design 5. Extensive analyses of phenotypes in the morphological level and molecular level; mouse phenotype rescue study.

6. Gene editing techniques: SiRNA & RNAi and morpholino in vivo and in vitro study

7. Histology study: Frozen and paraffin embed section immuno-staining (multicolor) to detect protein expression; whole mount or section in situ to detect RNA expression. Time lapse and confocal microscopy.

8. Zebra fish gene knock-down: synthesis cDNA by 5`Race RT-PCR, synthesis sense RNA; morpholino microinjection, JB4 section embedding and cutting, fish immunoassaying and whole mount in situ hybridization. Florence microscopy and confocal image.

9. Microsoft office (word document, excel, Powerpoint), Photoshop, FCS express and GraphPad Prism for presentation, good interpersonal skill, and hard working. Fed 2002- Jun 2006: Postdoctoral fellow, Renal Unit/Massachusetts General Hospital/ Harvard Medical School, Boston

ADPKD (autosomal cystic kidney disease) study by gene editing PKD1 gene to generate knock-out and transgenic mice models

A: Generated conditional knock out construct using molecular cloning and PCR techniques

B: Screen 800ES cells colonies using PCR and Southern blot techniques C: Mouse mating, crossing, and genotyping using PCR (daily) including PCR primers design

D: Analysis polycystic kidney phenotype by in situ hybridization and immunoassay and using fluorescent microscopy.

E: Dissected gene profile of PKD1 knock out mouse using Gene-chip (microarray) and confirmed RT-qPCR (total 63 genes), Western blot, IHC and ICC TAZ functional study in the zebra fish kidney development and cystic kidney disease A: Bioinformatic analysis and cloned zebrafish TAZ gene by 5`Race RT-PCR and molecular cloning techniques

B: Identified expression pattern of TAZ during zebrafish embryogenesis and kidney organogenesis by whole mount and section in situ hybridization C: Produced zebrafish cystic kidney by gene editing (design morpholino microinjection morpholino into Zebrafish oocyte

D: Histological analysis of phenotype of TAZ knock down morphant using JB4 section

E: Dissected the downstream signaling pathway of TAZ using in situ, QPCR and immunoassay

F: Created several mutated human TAZ cDNA and rescued the phenotype of TAZ morphant using Wild type and different mutated TAZ sense capped RNA as well as sense capped RNA from the downstream genes

Kidney stem cell project

A. Isolated kidney stem cell by multicolor fluorescence-activated cell sorting (FACs) B. Ischemia reperfusion injury and implantation of stem cell into kidney cortex, C. kidney organ culture and microinjection of stem cells into culture kidney, tissue processing (frozen and paraffin embedded tissue section) for X-gal staining and immuno-histochemistry, confocal imaging.

D. Kidney cell and Organ culture and microinjection of stem cells into culture kidney tissue

Jan 1998- Jan 2002: Ph.D candidate

Cell, molecular and Developmental Biology, Faculty of Medicine. Oulu University, Finland

A. Identified the expression pattern of Sprouty genes 1, 2 and 4 during mouse embryogenesis and kidney organogenesis

B: Generation hSprouty2 transgenic mouse and study sprouty2 function in the kidney, brain and eye development

C: Detected Wnt2b and type XVIII collagen signaling expression and induction of ureter branching during early kidney organogenesis. All above studies were published. The techniques used were primers design, PCR, genotyping by PCR, molecular cloning, RNA probe synthesis, whole mount and section in situ hybridization, frozen and paraffin embedded tissue section, IHC, cell culture, embryonic kidney, and lung culture

Technical Skills

Expertise and hands-on Experience in following techniques: 20 + cell, molecular and developmental biology including NGS 6+ years of pharmaceutical industry experience in producing monoclonal antibodies, polyclonal antibodies and recombinant proteins

Next Generation sequence (NGS): DNA & RNA extractions, cDNA synthesis, PCR

&RT-PCR, next-generation library prep, templating and PGM sequence, sequence data analysis

Immunology & Protein biology: design and construct antibodies or proteins in the expressing vector; transient transfection in mammalian cell lines and stable cell lines selection and production; protein (including antibodies) expression and purification in a mammalian cell line or E.Coli, ELISA, antibody epitope mapping, protein titration, pull-down assay or ligand binding assay such IP & CO-IP, ELISA/ELISPOT, ICC &IHC and Western blot and Coomassie blue staining; translation assays; protein purification (affinity chromatography) Cell culture: media preparation, subculturing adherent cells, and suspension cells, freezing cells, thawing frozen cells, cell counting; transient transfection in mammalian cell lines and stable cell lines selection and production

Molecular biology: extraction and purification of plasmid DNA, genomic DNA, total RNA; Bac clone and plasmid cloning; DNA sequencing; mutagenesis. cDNA synthesis, southern blot; primers design, RNA probe, and sense RNA synthesis, PCR, RT-PCR, qPCR, and RT-qPCR Histology and embryology: gene expression study (the whole mount in situ hybridization; radioactive and non-radioactive section in situ hybridization advanced immunoassaying, IHC); morphological study (section HE staining; LacZ staining, apoptosis, and proliferation); fluorescent microscopy; organ and tissue culture (mouse embryonic kidney and lung). Generation of transgenic and knock-out mice: design and construction of transgenic and knock out/in constructs traditional; multiple-step cloning, ES cell screening (PCR and southern blot); mouse husbandry (crossing and breeding); genotyping (PCR and southern blot); RT-PCR and microarray; extensive analyses of phenotypes in the morphological level and molecular level; mouse phenotype rescue study. Familiar with microscope and Florence microscope image system. Zebrafish gene knock-down: synthesis cDNA by 5`Race RT-PCR, morpholino microinjection, JB4 section embedding, and cutting, fish immunoassaying and in situ hybridization. Stem cell biology: stem cell isolation (FACs, multi-color Fluorescence-activated cell sorting and flow cytometry), ischemia-reperfusion injury and implantation of stem cell into kidney cortex, kidney organ culture and microinjection of stem cells into culture kidney, tissue processing, X-gal staining, and immunohistochemistry

Analysis data and presentation data using Microsoft office (word document, excel, Powerpoint), Photoshop.

Strong verbal and written communication skills, excellent presentation skills Strong interpersonal skills; independent working talent and a good team player. scientific critical thinking, troubleshooting, and strong analytical skills to evaluate complex information for the identification of critical scientific findings related to projects; updating literature and adapting to fast-paced working environment and carrying multi-tasks; preparing and ordering reagents and laboratory supplies; teaching and guiding Ph.D. students, research associates, and technicians Publications

Shaobing Zhang, Yanfeng Lin, Petri Itäranta, and Seppo Vainio. Expression of Sprouty genes 1, 2 and 4 during mouse organogenesis. Mechanism of Development. 109 (2001) 367-370.

Shaobing Zhang, Lijun Chi, Yanfeng Lin, Renata Prunskaite-Hyyrylainen, Reetta Vuolteenaho, Petri Itaranta, Seppo Vainio. Sprouty proteins regulate ureteric branching by coordinating reciprocal epithelial Wnt11, mesenchymal Gdnf and stromal Fgf7 signalling during kidney development. Development. 2004 Jul;131(14):3345-56.

Yanfeng Lin, Shaobing Zhang, Marko Rehn, Petri Itäranta, Juha Tuukkanen, Ritva Heljäsvaara, Hellevi Peltoketo, Taina Pihlajaniemi and Seppo Vainio

(2001) Induced repatterning of type XVIII collagen expression in ureter bud from kidney to lung type: association with sonic hedgehog and ectopic surfactant. Development, 128:1573-1585

Yanfeng Lin, Aiping Liu, Shaobing Zhang, Tarja Ruusunen, Jordan A. Kreidberg, Hellevi Peltoketo, Iain Drummond and SeppoVainio (2001) Induction Of ureter branching as a response to Wnt-2b signaling during early kidney organogenesis. Developmental Dynamics, 222(1): 26-39. Shaobing Zhang, Yanfeng Lin, Juha Tukkanen, Hellevi Peltoketo, Taina Pihlajaniemi and Seppo Vainio. Ureteric bud changes to early lung type branching pattern in ureteric bud lung mesenchyme heterotypic tissue recombinants based on the morphological analysis of the computer-processed image. Int.J.Dev Biol.47: 3-13 (2003)

Lijun Chi, Petri Itäranta; Shaobing Zhang, Seppo Vainio. SPROUTY2 is involved in male sex organogenesis by controlling fibroblast growth factor 9-induced mesonephric cell migration to the developing testis. Endocrinology. Pub date: 2006-05-06, Dol: 10.1210/en.2006-0299. Shaobing Zhang, Amin Arnaout. Konck down of TAZ signal in zebrafish down regulates PKD1 and led to polycystic kidney disease. Manuscript. Grace X. Li, Sherry Shaobing Zhang, Ren Liu, Bani Singh, Sukhmani Singh, I. Quinn, Gage Crump, Parkash S. Gill Biology Open 2021: bio.050096 doi: 10.1242/bio.050096 Published 21 July 2020. Tetraspanin18 regulates angiogenesis through VEGFR2 and Notch pathways

. Grace X Li, Sherry Shaobing Zhang, Ren Liu, Imran N. Parkash S. Gill1. EphB4-EphrinB2 are targets in castration resistant prostate cancer. Receptor tyrosine as a downstream effector PTEN deficient prostate cancer PhD thesis

Shaobing Zhang. Kidney development: roles of Sprouty, Wnt2b and type XVIII collagen in the ureteric bud morphogenesis. University of Oulu, Oulu Finland 2003. ISBN ***-**-****-8. URN: ISBN: 951-***-****. Honors

1998 CIMO Grant from Finnish Academy

Award from Faulty of Science, Oulu Univ., Finland

2002 Award from APURAHAHAKEMUS, Finland

Award from Finish Society of Sciences and Letters

The paper of Sprouty2 transgenic mouse was elected to be the best article in Finland

Award from Ruth L. Kirschstein National Research Service Award (NRSA) NIH K01 (Mentored Research Scientist Development Award) candidate References:

1. Michael Laptewicz

Sr Mgr R&D

Molecular Biology

Specialty Diagnostics Group

Clinical Next Generation Sequencing

Cell: 508-***-****

Email: Laptewicz, Mike ad2t3y@r.postjobfree.com 2. Anne Marie, Velasco Roth

Sr Director R&D

Molecular Biology

Specialty Diagnostics Group

Clinical Next Generation Sequencing

Cell: 858-***-****

Email: Velasco Roth, Anne Marie <ad2t3y@r.postjobfree.com> 3. Mayuri D Naidu

Sr Mgr R&D

Molecular Biology

Specialty Diagnostics Group

Clinical Next Generation Sequencing

Cell: 508-***-****

Email: Naidu, Mayuri D. <ad2t3y@r.postjobfree.com>

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