Laboratory Manager Quality Control

Scientist and Laboratory Manager for Cardiac Electrophysiology Lab

Research Laboratory Manager with experience in writing IACUC protocols, managing laboratory operations including the budget, ensuring quality control and safety compliance, developing policies and procedures, and ordering supplies.

Identify and address issues and deficiencies and implement effective solutions.

Ensure compliance with existing lab Standards Operating Procedures and assess the need for revisions and system improvement.

Responsible for QA/QC in the laboratory. Have worked extensively with the IACUC ( Institute Animal Care and Use Committee ) and EHS ( Environmental Health and Safety ) and Vet Resources on the University of MD campus keeping the lab and lab members compliant.

Scientist with experience in Immunofluorescent staining of both live and fixed cells, both primary culture and cell lines.

Fluorescence microscopy and confocal microscopy ( Zeiss LSM 510).

Tissue culture and aseptic technique for both primary adult and neonatal heart cells and cell culture lines.

Molecular biology techniques including PCR, western blots, DNA and RNA isolation, cloning and cell transfection.

-Meticulous record-keeping to maintain accurate and organized lab documentation

-Demonstrated critical thinking and problem-solving skills to address research challenges

-Competent in animal handling, including the care and maintenance of research animals

-Proficient in Microsoft Office suite, with skills in Excel, Word, and PowerPoint

-Website design and maintenance skills, including using Wix, Wordpress, Photoshop and other graphics software

-Proficiency in Canva for creating visually appealing graphics and presentations

-Excellent communication and team collaboration abilities in a research laboratory setting

Core Strengths:

Tracking expenditures and keeping budgets

Organized and efficient

Strong work ethic

Excellent written and oral communication skills

Immunofluorescence

Tissue culture of primary and cell culture lines.

Supervisory Experience of Research Assistants and Graduate students.

Professional Biologist/Biochemist -Basic Science Research Experience

2014-present – Research Laboratory Manager- University of Maryland, Department of Biomedical Engineering and Technology.

-Responsible for the writing of many IACUC protocols and amendments for the lab. Responsible for laboratory budget and expenditures reconciling this with our NIH grant budget using the Quantum analytics financial software at the University of MD.

Training and advising of laboratory staff. Provide technical advice and administrative support, maintain the lab, and trouble shoot problems on a daily basis. Keep accurate records and ordering of lab animals and supplies. Keep accurate records of data and protocols. Writing standard operating procedures for the lab. Manage laboratory operations, including the budget, keep accurate records of lab expenditures, ordering of all supplies and ensure quality control and safety compliance. Evaluate equipment to be ordered and draft standard operating procedures for that equipment. Work with IACUC, EHS and Veterinary Resources on campus to make sure the lab is compliant.

2010- 2014- Research Laboratory Supervisor – University of Maryland, Department of Biomedical Engineering and Technology.

- Fluorescence microscopy, Confocal Microscopy Zeiss LSM 510, Immunofluorescence staining of cardiac cell channels and proteins. Isolation of cardiac ventricular myocytes from adult rat using the Langendorff method and neonatal rat and mouse cell isolation and tissue culturing techniques. Tissue Culture Techniques: Include both primary cell culture of adult and neonatal rat and mouse ventricular myocytes, as well as working with different cell lines, such as HEK, COS, HUVEC, and Mesenchymal stem cells. Lipofectamine cell transfection in cardiac cells. Ordering of supplies and animals for the lab. Kept accurate records of data and SOP’s for the lab. Coordinate with Environmental Health and Safety (EHS), Veterinary Resources and Institute Animal Care and use Committee (IACUC) for the lab.

2000- 2009 - Research Laboratory Supervisor: University of Maryland Biotechnology Institute, Institute of Molecular Cardiology

- Fluorescence microscopy, Confocal Microscopy Zeiss LSM 510, Immunofluorescence staining of cardiac cell channels and proteins. Isolation of cardiac ventricular myocytes from adult rat using the Langendorff method and neonatal rat and mouse cell isolation and tissue culturing techniques. Supervised Senior Research Assistant and graduate students. Ordering of supplies and animals for the lab. Kept accurate records of data and SOP’s for the lab.

1993-2000 - Research Specialist: University of Maryland, Dept. of Physiology

- Isolation of cardiac ventricular myocytes from adult rat using the Langendorff method and neonatal rat and mouse cell isolation and tissue culturing techniques. PCR, Cloning, DNA and RNA Isolation, Western Blots. Fluorescence microscopy, Confocal Microscopy Zeiss LSM 510, Immunofluorescence staining of cardiac cell channels and proteins. Designed and implemented new/novel protocols for the lab that students and post docs have used in their research. Ordering of supplies and animals for the lab. Kept records of animal colonies, including mouse breeding colony. Kept accurate records of data and SOP’s for the lab.

ADDITIONAL RELEVANT EXPERIENCE:

Senior Lab Technician: Johns Hopkins University, Dept. of Anesthesiology

Performed laboratory research on microwave irradiation effects on capillary endothelial cells in rats and athermal alterations in the structure of the canalicular membrane and ATPase activity induced by thermal levels of microwave irradiation. Microwave exposure on the temperature dependent response of hepatic bile flow rate in rats.

Senior Research Assistant: University of Maryland, Dept. of Physiology

Isolation of cardiac ventricular myocytes from adult rat using the Langendorff method and neonatal rat and mouse cell isolation and tissue culturing techniques for primary cell cultures of rat neonatal cells. PCR, Cloning, DNA and RNA Isolation, Western Blots. Ordering of supplies for the lab. Kept accurate records of data and SOP’s for the lab.

Education

BA Biology, Notre Dame of Maryland University

MS Biochemistry, University of Maryland, Baltimore

Publications

Dushon Devere Riley, Terry B. Rogers, Aarif Y. Khakoo, Cecilia A. Frederick, Shibani Pati, Shirley A. Gaa, W. Jonathan Lederer 2010 Mesenchymal Stem Cells Protect Cardiomyocytes. Biophysical Journal (Impact Factor: 3.67). 01/2010; 98(3):718-. DOI:10.1016/j.bpj.2009.12.3934

Salnikov V, Lukyanenko YO, Frederick CA, Lederer WJ, Lukyanenko, V. (2007)

Probing the outer mitochondrial membrane in cardiac mitochondria with nanoparticles. Biophysical Journal 2007 Feb1; 92(3) 1058-77.

Desmin and Calcium Signaling in Heart (2003) K.W. Dilly, S. Guatimosim, C.A. Frederick,, N. Weisleder, E, Kranias, Y, Capetanaki, and W.J. Lederer. Circulation Feb. 2004

Jador Dos Santos Cruz, L.F. Santana, Cecilia Frederick, Lori L. Isom, Jyoti Dhar Malhotra, Laura N. Mattei, R.S. Kass, j-Xia, R.H. An, W.J. Lederer (1999) Whether Slip Mode Conductance Occurs. Science 284: April 30, 1999: 711a-6-711a-13.

Lederer, W.J., He,S., Luo,S., duBell, W., Kofuji,.P., Kieval,R. Neubauer, Cecilia Frederick, Ruknudin, A., Cheng, H., Cannell, M.B., Rogers, T.B., Schulze, DH. (1996)

The Molecular Biology of the Na/Ca Exchanger and it’s functional roles in heart, smooth muscle cells, neurons, glia, lymphocytes, and non-excitable cells. In: Sodium Calcium Exchange. (Annals of the New York Academy of Science.779:7-17)

Phelan, A.M., Neubauer, Cecilia Frederick, Timm, R. Nierenberg, J., Lange D.G. (1994) Athermal alterations in the structure of the canalicular membrane and ATPase activity induced by thermal levels of microwave irradiation. Radiation Research 137:

52-58.

Abdelatif, M.M., Neubauer, Cecilia Frederick, W.J. Lederer, Rogers, T.B. (1991)

Angiotensin-induced desensitization of the phoshoinositide pathway in cardiac cells occurs at the level of the receptor. Circulation Research 69: 800-809.

Neubauer, Cecilia Frederick, Phelan, A.M. Kues, H. Lange, D (1990) In vivo microwave exposure activates pinocytotic-like uptake mechanisms in rat cortical capillary endothelial cells. Journal of Bioelectromagnetics, 11:261-268

Abstracts and Meetings Attended

Hong Song,Ling Chen,Jane C Lee,Michael I Kotlikoff,W Jonathan Lederer,Cecilia A Frederick,Eiji Karaashima,Willaim C Randall,Mordecai P Blaustein 2010 N-Terminal α2 Na+ Pump Constructs are Dominant Negative for Native α2 Na+ Pumps in Smooth, but not Cardiac Muscles, and Increase Salt Sensitivity FASEB Meeting April 2010

Mesenchymal Stem Cells Provide Benefit to Injured Cardiac Ventricular Myocytes Through a Paracrine Mechanism. T.B. Rogers, A. Y. Khahoo, S. Pati. G. Hall, R. Wardlow, S. Gaa, D. Riley, CA Frederick, W.J. Lederer. World Stem Cell Summit Meeting Sept. 2009.

Mesenchymal Stem Cells Stimulate Protective Genetic Reprogramming of Injured Cardiac Ventricular Myocytes. T.B. Rogers, A.Y. Khahoo, S.S Patel, S.Pati, G.Hall, S.Gaa, D.Riley, CA Frederick, W.J. Lederer. World Stem Cell Summit Meeting Sept. 2009.

Desmin and Calcium Signaling in Heart. K.W. Dilly, Silvia Guatimosim, C.A. Frederick, N. Weisleder, E. Kranias, Y. Capatanaki, and W.J. Lederer. Biophysical Journal and Meeting Feb. 2004.

Distribution of the Na/Ca Exchanger in Hypertrophic and Failing Rat Cardiac Myocytes. K.W. Dilly, A.M. Gomez, Cecilia Frederick Neubauer, D.H. Schulze, R. Altschuld, and W.J. Lederer. Biophysical Journal and Meeting Feb, 1997.

Deletion of the alternatively spliced region of the Na/Ca Exchanger, NCX1, reduces functional activity. S.Luo, C. Frederick Neubauer, A. Ruknudin, S. He, W.J. Lederer, and D.H. Schulze. Biophysical Journal and Meeting Feb. 1997.

The Human Cardiac Na/Ca Exchanger Expressed in Sf9 cells. M. Egger, P.Lipp, A. Ruknudin, C. Frederick Neubauer, W.J. Lederer,, D.H. Schulze, and E.Niggli. Joint Congress of the Swiss and German Physiological Societies, Zurich Switzerland. March 1996.

The Effects of TPA on I ca and Excitation- Contraction Coupling in Rat heart cells changes during development. M.S. Kirby, A. Lokuta, S. Gaa, C. Frederick Neubauer,, T.B. Rogers, and W.J. Lederer. American Physiological Society Conference (1992)

Acute Low dose Microwave Irradiation alters Methyl-Naltrexone potency in mice. David G. Lange, Anne M. Phelan, Cecilia A. Frederick, Henry Kues, and Raymond M.Quock. Bioelectromagnetics Society Meeting (June 1988)

Acute low level pulsed Microwave alters In-Vivo Micro-pinocytotic uptake of Rhodamine- Ferritin Complex by CNS Capillary Endothelial Cells (CEC). Cecilia A. Frederick, and David G. Lange. June 1988)

Effects of Chronic Mircowave Exposure on the Temperature Dependent response of Hepatic Bile Flow Rate in Rats. D.G. Lange, C.A. Frederick, J. Nierenberg, R.R. Timm. (April 1986)

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