Jessica Otte

*** ****** ***

Phoenixville, PA ***6003

cell: 215-***-****

ad2hd4@r.postjobfree.com

I seek a position where my technical experience will be an asset to research or clinical diagnostics. I’ve spent thirty-five years in combined managerial and technical roles to further neurovirology, cancer research, drug discovery, and clinical diagnostics. I thrive in a busy environment, and I like to work.

Professional summary

Non-clinical Histology supporting pharmaceutical pre-clinical studies.

Clinical histology supporting patient diagnosis of gastrointestinal sampling.

In vivo pharmacology supporting drug discovery in pharmaceutical cancer targets.

Small molecule drug discovery/in vitro assays for human disease markers based on ubiquitin pathway.

Neuroscience/Neurovirology: technical contribution to translational research in vivo/in vitro on neurological disorders and human neurotropic viruses

In vivo/in vitro molecular biology research; human neurotropic viruses

Technical proficiency & experience

Rodent and non-human primate necropsy.

Rodent, non-human primate, and human GI clinical sample histology.

Whole slide scanning and archiving.

LIMS sample management.

HTRF, cell proliferation, ELISA, MSD, PD marker, cytotoxicity assays

Animal drug dosing, venipuncture, tumor cell culture & growth assessment.

Primary tissue culture, neuronal and neuroglial isolation and culture, cell type validation.

Transformed cell line culture, expansion, and banking.

SDS-PAGE, nucleic acid blotting.

PCR, RT-PCR, qPCR.

Regulatory & safety assessments and documentation.

Shipment of hazardous and biohazard materials.

Positions held:

June 16, 2022, to current: Senior Scientist Non-Clinical Histology, GSK, Collegeville, PA 19426

Preclinical study support. Proficient in rodent and non-human primate necropsy for histology on discovery studies, candidate selection, and toxicity studies. Proficient in tissue trimming, fixation, processing, embedding, microtomy, routine and special staining, ISH/RNAScope, automated IHC. Scanning of whole slides to report to pathologists via Hamamatsu Nanozoomer S360 and P250. Provantis study/protocol management. Signals and other ELN notebooks. LIMS assessment team.

Experience with Leica Bond and Ventana Discovery Ultra automated stainers, Ventana HE600 automated H&E staining, VIP automated tissue processors, Thermo-Fisher, Leica, Microm analog and semi-automated rotary microtomes, Leica cryostat.

Regulatory & safety document management.

Safety rep for IVIVT Non-Clinical Histology

Safety rep for UP02 GSK Building Two second floor including Clinical and Non-Clinical Histology, Discovery Analytical Purification, Preparative Chromatography, and Galvani Bioelectrics.

Owner/emergency contact for low temperature storage units and ambient storage room for slides and paraffin blocks GSK IVIVT/Non-Clinical Histology & LKSM Dept Neuroscience

Awards/Recognition

30 Mar 2023

Bronze recognition for volunteering as Upper Providence Building 2 2nd floor safety lead, implementation of changes that will be of benefit to the floor and to the building itself.

01 Jun 2023

Bronze recognition for support in preparation for the UP Entity Audit and continued dedication and leadership to drive safety at the site and support employee and environmental safety.

17 Nov 2023

Bronze recognition for delivering slides 10 days early during a period of uncertainty and restructuring; diligence, effort and teamwork greatly appreciated. This action enabled pathologists to deliver data to the CD40 team on time (necessary for CTLO) around prior commitments to Bepirovirsen, which would not have been possible on the original timelines.

28 Nov 2023

Bronze recognition for my role on the UP02 safety committee, essential to the ongoing effort to keep labs safe, and personnel working in a safe environment.

March 2021 – June 2022 Histology Technician US Digestive Health, Exton PA

Clinical GI histology for pathological diagnoses.

Proficient in accessioning, grossing, processing, embedding, microtomy, and staining for patient sample pathology on GI biopsy, endoscopy, colonoscopy, routine or symptom-based GI tract sampling and other procedure material for diagnosis. Processing lead; VIP and Epredia Excelsior automated tissue processors. Accessioning and embedding as needed, daily grossing/LIMS data entry, microtomy, and routine staining; manual or automated H&E, H. pylori, Alcian blue, PAS, and other special stains, immunohistochemistry on a variety of viral and cellular protein markers.

VitalDX / VitalAxis LIMS sample management.

Equipment validation and management.

July 20, 2020-March 10, 2021: Senior Staff Scientist, In Vivo Pharmacology Incyte, Wilmington DE

Cancer-target drug discovery. HTRF assay, cell proliferation, ELISA.

Animal tumor cell culture for implantation; oral, IV, IP, sub-cutaneous dosing of drug compounds in mice.

Tumor growth assessment, tissue harvest, rodent necropsy.

Western blots, MSD assays (Mesoscale Discovery) for a variety of targets/markers.

2018-July 2020 Manager, R&D, Progenra, Inc., Malvern, PA

Small molecule/drug discovery: Mammalian cell culture for in vitro assays; PD marker assays, cytotoxicity assay, in vitro protein degradation and expression. Ubiquitin pathways, USP7, Parkin, DUbs, leverage of the ubiquitin proteasome system and cellular protein regulation, in the areas of oncology, inflammatory diseases, neurological diseases, particularly Parkinson’s, and exploiting the ubiquitin proteasome system for rapid development of Covid-19 therapeutics.

2006- 2018 Research Coordinator/Comprehensive NeuroAIDS (CNAC) Lab Manager Department of Neurovirology and Center for Neurovirology, Temple University Lewis Katz School of Medicine, Philadelphia, PA

1999-2006 Center Manager, Department of Biology, College of Science & Technology

Temple University, Philadelphia, PA

1998-1999 Center Manager, Center for NeuroVirology

MCP-Hahnemann University, Philadelphia, PA

1997-1998 Lab Manager, Center for NeuroVirology

Allegheny University of the Health Sciences, Philadelphia, PA

1994-1997 Lab Manager, Department of Biology, Molecular Neurovirology Section

Thomas Jefferson University, Philadelphia, PA

1989-1994 Research Technician I, II, III, and Senior Technician progressively

The Wistar Institute of Philadelphia, Philadelphia, PA

Neuroscience/Neurovirology: Technical contribution to translational research utilizing experimental animal models of neurological disorders including demyelination, neurodegeneration, brain tumors, neuroproliferation and neuroinflammation. Technical proficiency in areas as outlined below. The research goal is understanding biological events involved in control of neural cell function, growth, and differentiation. To achieve this goal, we use human neurotropic viruses, including JC virus and HIV-1, both of which greatly impact on the normal function of a variety of neural cells, as probes to determine the mechanisms involved in the control of gene expression and signal transduction in the brain. Recent focus of HIV-1 work is centered on DNA excision and HIV-1 gene editing using CRISPR/Cas9.

Virology techniques: Proficient in propagation, titration, purification, and in vitro infection models at BSL2 and BSL2+ levels including HIV-1, JC virus, BK virus, Hepatitis B virus, Hepatitis C virus, Herpesvirus, Varicella Zoster Virus, and Zika virus.

Molecular biology techniques: Proficient in nucleic acid isolation and purification, PCR, qPCR, cloning, agarose gel electrophoresis, Southern and Northern blot hybridization, protein isolation and purification, Western blotting, ELISA.

Cell culture techniques: Proficient in isolation, purification, and banking of primary human fetal glial cultures including primary astrocytes, microglia, brain derived endothelial cells, oligodendroglia, neurons from human fetal brain tissue and mouse/rat embryonic brain tissue. Proficient in maintenance and propagation, transfection, infection of various primary cell cultures and transformed cell lines. Proficient in blood cell isolation: leukocytes, monocyte-derived macrophages.

Animal models: Proficient in handling of mice, rats, hamsters, and rabbits. Proficient in transgenic and knockout mouse colony management and husbandry including colony breeding and maintenance, genotyping, euthanasia, and tissue harvesting, routine injections and bleeds. Proficient in BSL2 and BSL2+ animal models, i.e. treatment of mice with viral vectors including lentivirus, adenovirus, adeno-associated virus, and infection of mice with HIV-1. Necropsy and tissue trimming of rodents and non-human primates for histology following drug discovery, candidate selection, and toxicity studies.

Animal injections/drug dosing intraperitoneal, subcutaneous, tail vein injection, gavage. Animal ID microchipping.

Regulatory compliance: Creation, review, and renewal of protocols, SOPs, ECPs, and all other regulatory documents related to Institutional Biosafety Committee (IBC), Institutional Review Board (IRB), Environmental Health and Safety (EHS), Radiation Safety, hazardous chemical inventory, DEA research license, licensed for national and international infectious materials transfer shipping, research animal shipping. Creation and renewal of Risk Assessments for non-clinical histology labs.

Core facility/resources management: Responsible for management of neuroscience cell culture repository, plasmid/DNA repository, human brain tumor bank repository, transgenic mouse colony tissue repository, neuropathology tissue repository. Glasswash and sterilization, equipment maintenance, service contracts and preventive maintenance manager.

Sterilization of animal cages, water & bottles, and bedding.

Sterilization of BL2+ waste for disposal or tools and rotors for use in BL2+ facility.

Sterilization of experimental viral infected rodent tissue and remains for disposal.

SporeCheck monitoring of autoclaving efficiency in reagents, tools, animal cages and water.

Hycheck monitoring of molecular biology labs, tissue culture suites and animal housing rooms for cleanliness and bacterial contamination.

Training and supervision: Manager and trainer for BSL2+ tissue culture facility and Neuropathology Core Facility; supervisor to lab aides.

Manager, supervisor, trainer for glasswash and sterilization in the Department of Neuroscience at Lewis Katz School of Medicine, Temple Medical School. Autoclaving of reagents, bacterial growth media, infectious animal tissues, and instruments.

Publications:

Jessica A. Otte - Google Scholar

Barbe MF, Krueger JJ, Loomis R, Otte J, Gordon J. Memory deficits, gait ataxia

and neuronal loss in the hippocampus and cerebellum in mice that are heterozygous for Pur-alpha. Neuroscience. 2016 Nov 19;337:177-190. doi:

10.1016/j.neuroscience.2016.09.018. Epub 2016 Sep 17. PubMed PMID: 27651147;

PubMed Central PMCID: PMC5458736.

Sariyer IK, Sariyer R, Otte J, Gordon J. Pur-Alpha Induces JCV GeneExpression and Viral Replication by Suppressing SRSF1 in Glial Cells. PLoS One. 2016 Jun 3;11(6):e0156819. PMID: 27257867.

Kaminski R, Bella R, Yin C, Otte J, Ferrante P, Gendelman HE, Li H, Booze R, Gordon J, Hu W, Khalili K. Excision of HIV-1 DNA by gene editing: a proof-of-concept in vivo study. Gene Ther. 2016 May 19. doi: 10.1038/gt.2016.41. PMID: 27194423

Gordon J, Sariyer IK, De La Fuente-Granada M, Augelli BJ, Otte J, Azizi SA,

Amini S, Khalili K, Krynska B. Neural Crest Cells Isolated from the Bone Marrow

of Transgenic Mice Express JCV T-Antigen. PLoS One. 2013 Jun 21;8(6):e65947. doi:

10.1371/journal.pone.0065947. Print 2013. PubMed PMID: 23805194; PubMed Central

PMCID: PMC3689770.

Beltrami S, Branchetti E, Sariyer IK, Otte J, Weaver M, Gordon J. Neurofibromatosis type 2 tumor suppressor protein, NF2, induces proteasome-mediated degradation of JC virus T-antigen in human glioblastoma. PLoS One. 2013;8(1):e53447. PMID: 23308224.

Mishra M, Del Valle L, Otte J, Darbinian N, Gordon J. Pur-alpha regulates RhoA developmental expression and downstream signaling. J Cell Physiol. 2013 Jan;228(1):65-72. PMID: 22553010.

Gordon J, Sariyer IK, De La Fuente-Granada M, Augelli BJ, Otte J, Azizi SA, Amini S, Khalili K, Krynska B. Neural Crest Cells Isolated from the Bone Marrow of Transgenic Mice Express JCV T-Antigen. PLoS One. 2013 Jun 21;8(6):e65947. doi: 10.1371/journal.pone.0065947. Print 2013. PMID: 23805194.

Otte, J., Safak, M. and Khalili, K. 2010. Polyomaviruses. Topley and Wilson's Microbiology and Microbial Infections. DOI: 10.1002/978**********.taw0230. 2010 John Wiley & Sons, Ltd.

Tremolada S, Akan S, Otte J, Khalili K, Ferrante P, Chaudhury PR, Woodle ES, Trofe-Clark J, White MK, Gordon J. Rare subtypes of BK virus are viable and frequently detected in renal transplant recipients with BK virus-associated nephropathy. Virology. 2010 Sep 1;404(2):312-8. doi: 10.1016/j.virol.2010.05.012. Epub 2010 Jun 15. PMID: 20554301.

Darbinian N, Cui J, Basile A, Del Valle L, Otte J, Miklossy J, Sawaya BE, Amini S, Khalili K, Gordon J. Negative regulation of AbetaPP gene expression by pur-alpha. J Alzheimers Dis. 2008 Sep;15(1):71-82. PMID: 18780968.

Darbinian-Sarkissian N, Darbinyan A, Otte J, Radhakrishnan S, Sawaya BE, Arzumanyan A, Chipitsyna G, Popov Y, Rappaport J, Amini S, Khalili K. p27(SJ), a novel protein in St John's Wort, that suppresses expression of HIV-1 genome. Gene Ther. 2006 Feb;13(4):288-95. PMID: 16251997.

Shollar D, Del Valle L, Khalili K, Otte J, Gordon J. JCV T-antigen interacts with the neurofibromatosis type 2 gene product in a transgenic mouse model of malignant peripheral nerve sheath tumors. Oncogene. 2004 Jul 15;23(32):5459-67. PMID:15133494.

Khalili K, Del Valle L, Muralidharan V, Gault WJ, Darbinian N, Otte J, Meier E, Johnson EM, Daniel DC, Kinoshita Y, Amini S, Gordon J. Puralpha is essential for postnatal brain development and developmentally coupled cellular proliferation as revealed by genetic inactivation in the mouse. Mol Cell Biol. 2003 Oct;23(19):6857-75. PMID: 12972605.

Radhakrishnan S, Otte J, Enam S, Del Valle L, Khalili K, Gordon J. JC virus-induced changes in cellular gene expression in primary human astrocytes. J Virol. 2003 Oct;77(19):10638-44. PMID: 12970448.

Khalili K, Del Valle L, Otte J, Weaver M, Gordon J. Human neurotropic polyomavirus, JCV, and its role in carcinogenesis. Oncogene. 2003 Aug 11;22(33):5181-91. Review. PMID: 12910255.

Croul S, Otte J, Khalili K. Brain tumors and polyomaviruses. J Neurovirol. 2003 Apr;9(2):173-82. Review. PMID: 12707848.

Gordon J, Del Valle L, Otte J, Khalili K. Pituitary neoplasia induced by expression of human neurotropic polyomavirus, JCV, early genome in transgenic mice. Oncogene. 2000 Oct 5;19(42):4840-6. PMID: 11039901.

Krynska B, Del Valle L, Gordon J, Otte J, Croul S, Khalili K. Identification of a novel p53 mutation in JCV-induced mouse medulloblastoma. Virology. 2000 Aug 15;274(1):65-74. PMID: 10936089.

Tretiakova A, Otte J, Croul SE, Kim JH, Johnson EM, Amini S, Khalili K. Association of JC virus large T antigen with myelin basic protein transcription factor (MEF-1/Puralpha) in hypomyelinated brains of mice transgenically expressing T antigen. J Virol. 1999 Jul;73(7):6076-84. PMID: 10364361.

Krynska B, Otte J, Franks R, Khalili K, Croul S. Human ubiquitous JCV(CY) T-antigen gene induces brain tumors in experimental animals. Oncogene. 1999 Jan 7;18(1):39-46. PMID: 9926918.

Gordon J, Krynska B, Otte J, Houff SA, Khalili K. Oncogenic potential of human neurotropic papovavirus, JCV, in CNS. Dev Biol Stand. 1998;94:93-101. Review. PMID: 9776230.

Krynska B, Gordon J, Otte J, Franks R, Knobler R, DeLuca A, Giordano A, Khalili K. Role of cell cycle regulators in tumor formation in transgenic mice expressing the human neurotropic virus, JCV, early protein. J Cell Biochem. 1997 Nov 1;67(2):223-30. PMID: 9328827.

Rencic A, Gordon J, Otte J, Curtis M, Kovatich A, Zoltick P, Khalili K, Andrews D. Detection of JC virus DNA sequence and expression of the viral oncoprotein, tumor antigen, in brain of immunocompetent patient with oligoastrocytoma. Proc Natl Acad Sci U S A. 1996 Jul 9;93(14):7352-7. PMID: 8692997.

Chang CF, Otte J, Kerr DA, Välkkilä M, Calkins CE, Khalili K. Evidence that the soluble factors secreted by activated immune cells suppress replication of human neurotropic JC virus DNA in glial cells. Virology. 1996 Jul 1;221(1):226-31. PMID: 8661431.

Wroblewska Z, Valyi-Nagy T, Otte J, Dillner A, Jackson A, Sole DP, Fraser NW. A mouse model for varicella-zoster virus latency. Microb Pathog. 1993 Aug;15(2):141-51. PMID: 8255207.

Wroblewska Z, Spivack JG, Otte J, Steiner I, Brown M, MacLean A, Fraser NW. The HSV-1 latency associated transcript (LAT) variants 1704 and 1705 are glycoprotein C negative. Virus Res. 1991 Jul;20(2):193-200. PMID: 1659061.

Education:

Bachelor of Science, Microbiology, Penn State University, University Park, PA 1989

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