GAUTAM ADHIKARY, PH.D., M.S.

CITIZENSHIP: USA

ad21tw@r.postjobfree.com 440-***-****

RESEARCH SCIENTIST

Accomplished scientist with extensive research experience in biochemistry, molecular biology, cell biology and cancer biology focusing on the role of cancer stem cells, tumor suppressors, oncogenes, mechanisms of cancer development and prevention.

Proven track record of productivity and innovation resulted in the publication of 70 articles in renowned journals.

Proactively partner with key internal stakeholders, including diverse group of scientists and corporate partners, on all mission-critical and strategic priorities and ensure collaboration across cross-functional teams.

Demonstrate excellent strategic planning, prioritization, and communication competencies while achieving critical deadlines and maintaining highest quality output.

Solid work ethic with capacity to function well under pressure and display a high level of competence in a rapidly evolving work environment.

Additional experience in mentoring in mentoring PhD students and post-doctoral fellows.

CORE COMPETENCIES

Good Laboratory Practice (GRP), growth and maintenance of primary human cell line and various cancerous cell lines.

Purifying adenovirus, plaque assay and cell signaling study using adenovirus mediated expression of signaling components.

DNA, RNA isolation, qRT-PCR, immunology, Oncology, immunoblot, ELISA, MTT assays, fluorescence microscopy and immunohistochemistry.

Handling of mice, colony maintenance of transgenic and knockout mice, genotyping.

Tumor xenograft study, designed drug effect on mice xenograft tumor by IP injection or by oral gavage, mice tail vein injection of NK cells.

Spheroid culture to enrich cancer stem cells, isolation, characterization by ALDEFLORE assay and flow cytometry.

CRISPR/Cas9 gene knockdown, screening to isolate single knockdown clone.

Independently designing and conducting experiments, analyzing results and presenting data findings.

Reviewing research papers and creating research proposals for grant funding.

Analysis of observed data by using Sigma Plot, Excel, CorelDRAW, and Power Point.

CAREER EXPERIENCE

UNIVERSITY OF MARYLAND, Baltimore, MD, 2007 – 2023

Faculty / Assistant Professor, Department of Biochemistry & Molecular Biology (2015 – 2023)

RESEARCH PROJECT HIGHLIGHTS

Held accountability for performing research on human mesothelioma cancer cells, showing that cancer cells are more addicted with amino acid glutamine (restriction, inhibition of glutamine entry in cancer cells). Performed various mesothelioma cell cultures and maintained and grew cell lines. Monitored glutamine restriction on cell growth, morphology change, spheroid growth and screened / isolated CRISPER/cas9 mediated SLC1A5 knock out single clones. Performed mice injection of control and glutamine transporter knockout cells.

Research showed that glutamine cell transporter gene significantly reduced tumor burden in mice (findings published in Molecular Carcinogenesis, 2023).

Effectively coordinated all research project logistics, including definition, specification development, risk management, change requests, progress reporting, communication, and scope management.

Conducted research on squamous carcinoma cancer cells to show that injection of human natural killer cell (NK) to mice suppressed tumor growth. Cultured human NK cells and squamous carcinoma cells, monitored effect of NK cells on SCC-13 cells growth, spheroid formation and invasion. Designed protocol to study the effect of NK cells on SCC-13 tumor growth in mice and optimized number of NK cells needed to observe tumor suppression.

Natural killer cell (NK) and maintained by Thermo Fisher Scientific form human donors suppressed squamous carcinoma cell growth, induced apoptosis, and reduced tumor burden in tumor Xenograft study. (Published in Molecular Carcinogenesis, 2023).

Facilitated management of entire research product lifecycle, including data / requirements gathering, collation, prioritization, and quality monitoring.

Previous Professional Career History:

University of Maryland, Department of Biochemistry & Molecular Biology, Baltimore, MD, Faculty (Research Associate) (2007 – 2015)

Case Western Reserve University, Cleveland, OH, Faculty (Research Associate)

Cleveland Clinic Foundation, Cleveland, OH, Post Doc Fellow

EDUCATION

Ph.D. (Biochemistry), Jadavpur University, Bose Institute, Calcutta, India

M.S. (Biochemistry), Calcutta University, India B.S. (Honors in Chemistry), Calcutta University, Calcutta, India

PATENT

United States Patent: US011246856B2

Eckert RL, Fisher M, Grun D, Adhikary G, Xu W. Method for treating BRAF inhibitor resistant melanoma using verteporfin (2022)

AWARDS / RECOGNITION

Qualified Nation Educational Test for Research Admission conducted by (CSIR-UGC), Govt of India.

Junior Research Fellowship (JRF) Senior Research Fellowship (SRF)

RESEARCH EFFORTS / SELECTED PUBLICATIONS

Adhikary G, Heipertz EL, Preradovic M, Chen Xi, Xu W, Newland JJ, Navjot K, Vemuri MC, Eckert RL (2023). Natural Killer cells suppress human cutaneous squamous cell carcinoma cancer cell survival and tumor growth. Mol Carcinog 62, 845-854.

Adhikary G, Shrestha S, Naselsky W, Newland JJ, Chen Xi, Xu W, Emadi A, Friedberg JS, Eckert RL (2023). Mesothelioma cancer cells are glutamine addicted and glutamine restrication reduce YAP1 signaling to attenuate tumor formation Mol Carcinog 62, 438-449.

Naselsky W, Adhikary G, Shrestha S, Chen Xi, Ezeka G, Xu W, Friedberg JS, Eckert RL (2022). Transglutaminase 2 enhances hepatocyte growth factor signaling to drive the mesothelioma cancer cell phenotype. Mol Carcinog 61, 537-548.

Adhikary G, Grun D, Alexander HR, Friedberg JS, Xu W, Keillor JW, Kandasamy S, Eckert RL (2018). Transglutaminase is a mesothelioma cancer stem cell survival protein that is required for tumor formation. Oncotarget, 9, 344**-*****.

Saha K, Adhikary G, Eckert RL (2016). MEP50/PRMT5 reduces gene expression by histone arginine methylation and this is reversed by PKC /P38 signaling. J Invest Dermatol 136, 214-224.

Adhikary G, Grun D, Balasubramanian S, Kerr C, Huang JM, Eckert RL (2015). Survival of skin cancer stem cells requires the Ezh2 polycomb group protein. Carcinogenesis 36, 800-810.

Saha K, Adhikary G, kanade SR, Rorke EA, Eckert RL (2014). P38 regulate p53 to control p21Cip1 expression in human epidermal keratinocytes. J Biol Chem 289, 114**-*****.

Adhikary G, Grun D, Kerr C, Balasubramanian S, Rorke EA, Vemuri M, Boucher S, Bickenbach JR, Hornyak T, Xu W, Fisher ML, Rekert RL (2013). Identification of a population of epidermal squamous cell carcinoma cells with enhance potential for tumor formation. PloS One 8, e84324.

Adhikary G, Chew YC, Reece EA, Eckert RL (2010). PKC- and-, MEKK-1. MEK-6, MEK-3, and p38- are essential mediators of the response of normal human epidermal keratinocytes to differentiating agents. J Invest Dermatol 130, 2017-2030.

Adhikary G, Sun Y, Pearlman E (2008). C-Jun NH2 terminal kinase (JNK) is an essential mediator of toll-like receptor 2-induced corneal inflammation. J Leukoc Biol 83, 991-997.

Adhikary G, Crish JF, Gopalakrishnan R, Bone F, Eckert RL (2005). Involucrin expression in the corneal epithelium: an essential role for Sp1 transcription factors. Invest Opthalmol Vis Sci 46, 109-120.

Sivasubramanian N, Adhikary G, Sil PC, Sen S. (1996). Cardiac myotrophin exhibits rel/NF-kB interacting activity in vitro. J Biol Chem 271, 2812-2816.

Full List of Publication available at:

https://www.ncbi.nlm.nih.gov/myncbi/gautam.adhikary.1/bibliography/public/

REFERENCES

References will be sent on request.

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