SACHI AHMED
Fremont, California 94555
Phone: 650-***-**** (C)
Email: ad1y1o@r.postjobfree.com
SUMMARY
Driven antibody production leader, with 20 years of experience in Biotechnology industry, currently leading downstream purification team of RA, SRA and associate scientist to support production of antibodies (IgGs, IgAs, IgMs and bispecifics). Specialized in analytical method development, in-vitro glycoengineering, antibody purification and characterization to advance the development of innovative biologics for oncology, autoimmune disorders and infectious diseases. Proven ability to lead antibody production teams, manage timelines based on projects, effectively communicate, solve analytical problems, present ideas, collaborate, and execute project plans.
TECHNICAL SKILLS
Downstream protein purification: Purification methods (IMAC, Ion Exchange, Affinity, SEC); AKTA systems (Pure, Avant, Prime, Start, Explorer), Ultrafiltration/Diafiltration/Tangential flow filtration, sterile filtration, formulation studies, concentrating and desalting proteins. Software: UNICORN
Analytical techniques: Size exclusion chromatography (SEC), Hydrophilic interaction liquid chromatography (HILIC), Dynamic Light Scattering (DLS); Differential Scanning Calorimetry (DSC), Bio-layer interferometry (BLI); Limulus amebocyte lysate (LAL) assay; instruments: HPLC/UHPLC, Nanodrop, Spectramax, OCTET. Software: Chemstation, Empower; FUTURE (Lunatic and Mass Photometer)
Upstream protein expression: Mammalian expression (Expi system, HEK293), Vicell, hemocytometer, Confocal/Light microscopes
Glycoengineering
Liquid Handlers: Hamilton Nimbus
Protein Biochemistry: Immunofluorescent staining, Immunofluorescent labeling, Fluorescence Size Exclusion Chromatography (FSEC), Electrophoresis, Western blotting
Cell Biology: Mammalian cell culture, T cell activation assay
ELISA (enzyme-linked immunosorbent assay)
Bacteria and Yeast: Cell culture, Plasmid DNA preparation, RT-PCR
Chemical Engineering design tools: EZ Solve, Maple, Hysis, DV Deck, Auto-cad, MS-Visio, Jmp
Programming Languages: HTML, C
Database Management: Benchling, BioELN, Research Project Management Database
EMPLOYMENT EXPERIENCE
Department of Antibody Sciences, IGM Biosciences Mountain View, California
Mar 2017-Dec 2023
Scientist II / Research Antibody Purification Group Leader (Mar 2021 – Dec 2023)
Responsibilities:
Generate Covid therapeutics antibodies, co-author of IgM based COVID therapeutics paper published in Nature
Lead downstream purification team of RAs, SRAs and associate scientists to support antibody production
Support protein production and characterization by SEC MALS, SEC HPLC, DLS, Western of IgMs, IgAs, IgGs for internal programs and external collaborators followed by sterile filtration
Performed DOE to do small formulation studies and freeze-thaw studies on final products
Manage protein production timelines and co-ordinate production requests with project teams
Process development and optimization of simultaneous multi-step purification platform to go from pilot scale to 500mgs scale up
Troubleshoot research-scale purification and optimize for high-throughput purification design
Help design and build downstream protein purification lab
Contribute to IND-enabling activities in assay development, generation and characterization
Glycan profiling of proof of concept and lead molecules
SOP generation and technical transfer documents to CROs
Involved in Culture building via the Activity Committee; contribution for the motivational section “Life in the time of Corona” in the monthly IGM Biosciences Newsletter “Affinity and Beyond”
Formed the “Women of IGM” group – which provides training and mentoring for career growth
Scientist I/Manager, Research Antibody Purification (Mar 2018 – Feb 2021)
Filed patent for In-Vitro Glyco Engineering of IgMs; developed IVGE methods to improve PK of cynomolgus monkey
Manage RA and provide training to RAs, assoc. scientists for protein production
Contributed to IND-enabling activities including generation and characterization of antibodies
Built protein purification schemas for Benchling (electronic lab notebook)
Analytical method development (HILIC based HPLC) to identify critical quality attribute in GMP
Associate Scientist (Mar 2017 – Feb 2018)
Establish a process for HPLC titer characterization and analysis by SEC HPLC and OCTET
Small-large scale purification of IgMs using AKTA systems (Purifier, Start, Prime, Avant)
Utilize Liquid Handler ie. Hamilton Nimbus for HTP protein purification with Phynexus tips
Characterization of IgM antibodies with Dynamic Light Scattering, SEC, westerns
Run Kappa, IgM ELISA, reverse based Lectin assay, PK ELISAs
Mammalian cell culture, Expi 293 transfections
SOPs for IGM analytical and purification processes
Train Research Associates
Department of Biochemistry, Abbvie Biotherapeutics, Redwood City, California Feb 2012 – Feb 2017
Scientist I/ Manager/ Internship Program Leader
Responsibilities:
Perform protein-A/G, His-tag purification of small to large scale antibodies, using AKTA PRIME and AKTA pure, titer measurement using BioRAD, BLItz, Octet
Managed Summer Intern (2015) and generated poster on “Optimization, Characterization and Quantification of Histidine Tagged Proteins”
Managed associate scientist
Characterize monoclonal antibody by SEC, UV, Endotoxin assay, SDS-PAGE, Western Blots
Removal of aggregates by polishing by preparative SEC on AKTA Avant
Conjugation of antibodies with Alexa Fluors, PE, pHrodo maleimides, Fab, Fab’2 generations
Assisting in characterization and production of Antibody Drug Conjugates
Developed ELISA protocols
Performed HEK 293 transfections
Designing and building purification lab
Collaborate across multiple groups and sites, and deliver regular presentations
Department of Pharmaceutical Sciences, Merck & Co., Inc., San Francisco, California Aug 2010 – Sep 2011 (laid off due to site closure)
Process Engineer
Responsibilities:
Formulated robust siRNA Lipid Nanoparticles (LNP) to support pre-clinical studies
Conducted formulation screening, lab-scale process development, in-process characterization, troubleshooting and optimization which included high-energy mixing, extrusion, tangential flow filtration, ultra-filtration and sterile filtration
Performed analytical assays like measuring Zeta Potential, formulation and stability studies, thermal transition temperature by DSC, Particle Size, siRNA Concentration using NanoDrop, Encapsulation and Endotoxin
Assisted the Project Management team to manage material/inventory requests, working collaboratively across sites, franchises to integrate activities to meet RNA Therapeutics objectives
Department of Process Engineering, Cholestech, Hayward, California May 2009 – Dec 2009 (laid off due to site closure)
Process Engineering Chemist II
Responsibilities:
Evaluated the Lipid Standardization Program (LSP) process by performing testing of LSP cassettes utilizing unknown CDC samples on selected LDX Analyzers and analyzing LSP data with DCAS Utilties and Jmp Softwares
Worked closely with the manufacturing and QA department for troubleshooting the device
Software Validation Plan and Report
Module owner for Multi-Analyte Controls III material stability study and range assignment testing
Initiating Document Change Notice (DCN) by creating DCN Packet including PFDs
Department of Process Development, Protein Design Labs, Inc., Fremont, California Mar 2006 – Jan 2007 (took 2 years off to raise newborn)
Biochemist/Development Associate
Responsibilities:
Trained Bioanalytical Development Group in analytical chemistry methods
Analyzed Monoclonal Antibodies (Mab) and reagents using Cation Exchange Chromatography, SDS-PAGE, UV spectroscopy
Assisted in Western Blotting and Capillary Gel Electrophoresis assay development for Mab characterization
Evaluated the stability of Mab Formulated Drug Substance in Hyclone bags
Department of Pharmacology, College of Medicine, University of South Alabama, Mobile, Alabama,
Jun 2004 – Sep 2005
Research Assistant
Responsibilities:
Project: Determining the optimal dose and the effects of vitamin folic acid
Developed, tested and ran HPLC assays for determining the level of Homocysteine in human plasma, the enzymatic activity of Dehydrofolate Reductase in both rat and human plasma and the elution times of Tetrahydrofolate in human plasma
Prepared plasma samples for free and total homocysteine analysis, and micronuclei
Laboratory of Pathology and Medicine, Samuel Lunenfeld Research Institute, Mount Sinai Hospital, Toronto, Canada, Sep 2001-Apr 2002
Research Assistant
Responsibilities:
Project: In-Vitro Degradation Studies of Calcium Polyphosphate Bone Scaffolds
Designed and performed Batch, CSTR experiments for Sulphates, Phosphates and Carbonates for finding dissolution rates of Poly-Phosphates
Conducted the experiment using bone scaffold materials in various fluids (Simulated Body Fluid, low pH) to mimic environment during bone dissolution
Measured calcium concentration using Atomic Absorption Unit and colorimeter
Controlling dissolution rates to find potential candidates for resorbable bone materials
Terrence Donnelly Cardiac and Vascular Biology Research Laboratories, St. Michael’s Hospital, Toronto, Canada, May 2001-Aug 2001
Research Assistant
Responsibilities:
Project: Analysis of aneurysmal tissue sections
Developing double immuno-fluorescent staining for subsets of lymphocytes
Prepared animal spleen for lymphocytes separation, extraction and cell culture
Used hemocytometer for cell count and studying cell proliferation rates under different cell culture media and used confocal/light microscope, SEM to examine aneurismal tissue sections
Department of Chemical Engineering and Applied Chemistry, University of Toronto, Toronto, Canada, May 2000-Aug 2000
Research Assistant
Responsibilities:
Project: Studying the parameters of Maragoni Flow
Analyzed and calculated Maragoni convection parameters using MOCA software
Used DV deck software to capture and superimpose convective flow images
Prepared technical reports
PUBLICATIONS, PATENTS and POSTERS
Zhiqiang Ku, Xuping Xie, Paul R. Hinton, Xinli Liu, Xiaohua Ye, Antonio E. Muruato, Dean C. Ng, Sujit Biswas, Jing Zou, Yang Liu, Deepal Pandya, Vineet D. Menachery, Sachi Rahman, Yu-An Cao, Hui Deng, Wei Xiong, Kevin B. Carlin, Junquan Liu, Hang Su, Elizabeth J. Haanes, Bruce A. Keyt, Ningyan Zhang, Stephen F. Carroll, Pei-Yong Shi & Zhiqiang. Nature volume 595, pages718–723 (2021). An Nasal delivery of an IgM offers broad protection from SARS-CoV-2 variants
Keyt B, Baliga R, Rahman S, Carlin K, Hinton P, Smith M, Saini A, Tran H, Peterson M. (2021) “Highly sialylated multimeric binding molecules” – US Patent No. WO/2021/141902- 15.07.2021
Dean Ng, Paul R. Hinton, Tim Luger, Sachi Rahman, Bruce Keyt, Ramesh Baliga. PEGS Boston 2019. Modulating Complement Dependent Cytotoxicity (CDC) of a Bispecific Therapeutics IgM by Fcm Engineering
Ramesh Baliga, Keyu Li, Marigold Manlusoc, Paul R. Hinton, Dean C. Ng, Madeline Tran, Bing Shan, Hai Lu, Avneesh Saini, Sachi Rahman, Yuan Cao, Chitra Saraiya, Marvin S. Peterson, Stephen F. Carroll, Daniel S. Chen and Bruce A. Keyt. AACR 2020 IGM-2323 Poster 5664. A Bispecific IgM Antibody Format for Enhanced T cell-Dependent Killing with Minimal Cytokine Release
Ramesh Baliga, Keyu Li, Marigold Manlusoc, Paul R. Hinton, Dean C. Ng, Madeline Tran, Bing Shan, Hai Lu, Avneesh Saini, Sachi Rahman, Yuan Cao, Chitra Saraiya, Marvin S. Peterson, Steven F. Carroll, Daniel S. Chen and Bruce A. Keyt American Society of Hematology Annual Meeting, Orlando, FL – December 7-10, 2019. IGM-2323: High Avidity IgM-based CD20xCD3 Bispecific Antibody for Enhanced T-Cell Dependent Killing with Minimal Cytokine Release
Bruce Keyt, Ramesh Baliga, Keyu Li, Marigold Manlusoc, Paul Hinton, Dean Ng, Madeline Tran, Bing Shan, Hai Lu, Sachi Rahman, Avneesh Saini, Yuan Cao, Chitra Saraiya, Marvin Peterson, Wayne Russell Godfrey, Steve Carroll. ASCO20 Virtual Scientific Program – May 29-31, 2020 Session: Developmental Therapeutics–Immunotherapy Abstract # e15007. Lymphoma cell-killing activity and cytokine release by CD20-directed bispecific IgM antibody-based T-cell engager (IGM-2323)
Paul R. Hinton, Dean Ng, Keyu Li, Madline Tran, Marigold Manlusoc, Sachi Rahman, Yasinee Ng, Tim Luger, Ramesh Baliga, Bruce A. Keyt. Antibody Engineering and Therapeutics Meeting, San Diego, December 2018. High Avidity Anti-CD20 IgM Antibodies have fast, efficient CDC Activity on Low Antigen Expressing cells.
Ramesh Baliga, Keyu Li, Marigold M. Boe, Paul R. Hinton, Madeline Tran, Tim W Luger, Dean C. Ng, Avneesh Saini, Sachi Rahman and Bruce A. Keyt. Antibody Engineering and Therapeutics Meeting, San Diego, December 2017. An IgM-based Bispecific Platform for Enhanced T-cell and Complement Dependent Cytotoxicity on Low Antigen Expressing Cells
Angus Sinclair, Dalya Rosner, Beatrice Wang, Tasnim Kothambawala, Ling Wang, Susan Calhoun, Avneesh Saini, Sachi Rahman, Ramesh Baliga, Bruce Keyt. Abstract No. P405. SITC Annual Meeting 2018. Nov 7-11 Washington D.C. Agonistic IgM Antibodies Targeting Immunostimulatory TNFRSF Members GITR and OX40 Enhance Immune Response beyond that of IgGs
Dean C. Ng, Keyu Li, Paul R. Hinton, Tim Luger, Madeline Tran, Marigold Boe, Sachi Rahman, Avneesh Saini, Bruce Keyt, Ramesh Baliga. Poster, PepTalk 2018. In Vivo Half-Life Extension of Bispecific Therapeutic IgM using J-Chain Fusions.
Rahman, S. (acknowledgment section) The Journal of Immunology, 198(11), 4502-4512 - April 2017 Murinization and H Chain Isotype Matching of the Anti-GITR Antibody DTA-1 Reduces Immunogenicity-Mediated Anaphylaxis in C57BL/6 Mice
PROFESSIONAL AFFILIATION
-Women in Bio -TechWomen -Silicon Valley Women in Engineering -Chan Zuckerberg Initative
EDUCATION
Bachelor of Applied Science, Chemical Engineering, September 1998-June 2002
University of Toronto, Toronto, Ontario, Canada
Biochemistry 322 and Biochemistry Laboratory 323, January 2004-May 2004
University of South Alabama, Alabama, USA
Biotechnology and Drug Development, March 2010- June 2010
Stanford University, California, USA
AWARDS AND SCHOLARSHIP
1) September 2001-January 2002- Engineering Dean's Honor List, Faculty of Applied Science and Engineering, University of Toronto
2) October 2001- Honorable Mention Research Award, Materials Research Society, Undergraduate Materials Research Initiative, North America
3) May 2001- Undergraduate Summer Research Fellowship, Dept. Chemical Engineering and Applied Chemistry, UofT
4) November 2000- J. Edgar McAllister Foundation Bursary, UofT
References available upon request