P a p a n i k o l a o u C V

*

Nikolaos A. Papanikolaou

Associate Professor of Biological Chemistry

Aristotle University of Thessaloniki School of Medicine Panepistimioupolis, Bldg 16a, 3rd Floor, Thessaloniki, Macedonia, 54124 Greece Phone: 231*******

Email: ad1wgo@r.postjobfree.com

LinkedIn: https://www.linkedin.com/in/npapanikolaou/ Education

B.S, 1987 Biology, Manhattan College, Riverdale, NY, USA M.S, 1992 Biochemistry & Molecular Biology, New York Medical College, NY USA Ph.D, 2000 Biochemistry & Molecular Biology, New York Medical College, NY USA Professional Experience

2023-to-date Associate Professor of Medical Biochemistry, Touro College of Osteopathic Medicine, New York, NY USA

2021-to-date Associate Professor of Biological Chemistry, School of Medicine, Aristotle University of Thessaloniki, Thessaloniki, Macedonia, Greece

2009-2021 Assistant Professor (tenured in 2014), School of Medicine, Aristotle University of Thessaloniki, Thessaloniki, Macedonia, Greece

2014-2015 Hopkins-Libra Visiting Professor, Department of Biological Chemistry, Johns Hopkins University School of Medicine & Institute for Cell Engineering, Baltimore, MD USA 2007-2009 Bionalyst, Ludwig Institute for Cancer Research, Branch at Memorial Sloan Kettering Cancer Center, New York, NY USA

2003-2007 Associate Scientist, Departments of Pathology, Genetics and Development, Columbia University College of Physicians & Surgeons, New York, NY USA 2000-2003 Ruth Kirschtein National Research Service Award Postdoctoral Fellow in Biophysics, Molecular and Developmental Biology of Cancer, Institute for Cancer Genetics, Department of Pathology, Columbia University College of Physicians & Surgeons, New York, NY USA

1992-1995 Molecular Biologist, Department of Cardiovascular Molecular biology, Lederle Labs, American Cyanamid, Pearl River, NY USA

1987-1992 Biochemist, Thyone (Health Maintenance Programs, Inc.) Corporation, Valhalla, NY, USA Research Interests

• Biochemistry and Biophysics of protein-protein interactions in cancer (phenotypic Switching, PS)

• Computational/bioinformatics analysis of biological networks in cancer

• Use of small molecules and natural product inhibitors of receptor tyrosine kinase interactomes to identify novel protein targets in cancer (with molecular docking

• Mapping oncoproteins to pathways using genomics/proteomics data and network analysis Honors & Awards

Sigma Xi, The Scientific Research Honor Society, Full Member, 2023 Who is Who in Medical Education (WWME), 2023

P a p a n i k o l a o u C V

2

Plenary Speaker & Invited panelist, Hellenic Society for Therapeutic Oncology, Greece, 2022 Invited panelist, Hellenic Society for Therapeutic Oncology, Greece, 2021 Plenary speaker, “From Driver Mutations to Driver Cancer Networks: Why We Need A New Paradigm”, Hellenic Society for Therapeutic Oncology, Greece, 2018 Invited panelist, Hellenic Society for Therapeutic Oncology, Greece, 2017 Plenary speaker, 5th International Conference on Computational Systems Biology”, Philadelphia, USA, 2016

Invited panelist, Colloquium on Emerging Metabolomics, Conference Proceedings, Ls Vegas, Nevada, USA, 2016

Libra-Hopkins Fellow, Johns Hopkins University School of Medicine, Baltimore MD USA, 2014-2015 Invited speaker, Division of Gastroenterology, SUNY at Stony Brook Medical School, 2010-2015 Recipient of Young Investigator Research Awards (# 88132, 87711 & 90924), University of Thessaloniki, Macedonia, Greece, 2011-2014

Invited speaker, “From Chemical to Systems Biology” 1st FP7-SEEDRUG, Dpts of Chemistry, Pharmacy and Medicine, University of Patras, 2012

Invited speaker, Laboratory of Clinical Oncology, University of Patras School of Medicine, Greece, 2011 Recipient of the Conrad N. Hilton Foundation (CNHF) / Hilton-Ludwig Metastasis Initiative Award (CRA- 2431.9-0-CB-3838), Ludwig Institute for Cancer Research Memorial Sloan Kettering Cancer Center, 2007-2011

Ruth L. Kirschtein National Research Service Award Post-Doctoral Fellowships (#CA09503-16, 17) in the Biophysics, Molecular, Cellular and Developmental Biology of Cancer Institute for Cancer Genetics Columbia University College of Physicians & Surgeons, New York, NY USA, 2001-2003 Research Fellowship in Cancer Prevention, Institute for Cancer Prevention Formerly American Health Foundation, AHF Valhalla, New York, USA, 1999-2000 Graduate Research Fellowship, New York Medical College, Department of Biochemistry & Molecular Biology, Valhalla, New York, USA, 1995-1999

Third Place Award, Ninth Annual Graduate Student Forum, New York Medical College, 1996 Second Place Award, Third Departmental Student Forum, New York Medical College, Department of Biochemistry and Molecular Biology, Valhalla, New York, USA, 1996 Dean’s list, Manhattan College, Riverdale, New York, USA, 1982-1984 Journal Editorial Boards

Editor, FRONTIERS in Molecular Biosciences-Protein Biochemistry for Basic and Applied Science Section Editor, Current Protein and Peptide Science: Protein-Ligand Interactions, Protein-Protein Docking Section Editor, Protein and Peptide Letters: Protein-Protein Interactions Editor, Journal of Biochemistry & Cell Biology

Section Editor, Biochemistry and Biophysics, Hippokratia Research funding

• Innovative bio-functional foods and products based on Greek endemic herbs: Development Analysis - Claiming - Exploitation: Analysis of the effect of bio-functional preparations on the expression of P a p a n i k o l a o u C V

3

oncogenic kinases in cell culture models with proteomics. Analysis and ID of novel protein hub interactions with cellular and computational biochemistry. Bio-Synergy Program: Co-investigator, (€ 400,000 Euros), 2023-2025: In silico identification of target proteins using inhibitors of receptor tyrosine kinase interactomes and small molecule natural products, 2024-2025

• Dissecting the logic of phenotypic switching: Computational and mathematical analysis of the yeast MAPK network using Dynamical Systems and Automaton theory: Collaborative project with Professors Rahonis and Rovithakis, Aristotle University Mathematics and Mechanical Engineering Departments, 2024-2025

• Systems-level analysis of the protein methylome in the C2C12 mouse myoblast cell differentiation model, Hopkins-Libra, Principal Investigator, 2014-2015, 2018-2022

• Analysis of Multipotency genes in Monocyte-derived Multipotential Stem Cells, Collaboration with Professor Georgios Koliakos and Biohellenica Inc., 2019-2023

• Functional mechanisms of ZNF217 transcription factor multiprotein complexes in differentiation, cancer and aging”, Principal Investigator, and Identification of short linear motifs and other sequence elements that regulate protein-protein interactions of 8-zinc transcription factors”, Principal Investigator, 2017-2020

• Regulation of MDM2 Function by the Putative Transcription Factor Oxidase, ZNF217/p110b in Cancer Cell Culture Models”, Program: Support for Young Investigators, Aristotle University of Thessaloniki Grant # 90924, Sole Principal Investigator (4,000 €), 2012-2014

• Dissection of the Role of Kinase CDK4 in Coupling Multiplication and Differentiation in Cultured Acute Promyelocytic Leukemia Cells, Program: “Support for Young Investigators”, Aristotle University of Thessaloniki Grant # 87711, Sole Principal Investigator (4,000 €), 2012-2014

• Dissection of the Role of Kinase CDK4 in Coupling Multiplication and Differentiation in Cultured Acute Promyelocytic Leukemia Cells, Aristotle University of Thessaloniki Grant # 88132, Program: “Support for Young Investigators”. Sole Principal Investigator (4,000 €), 2012-2013

• Inferring the Function of Cancer Testis Genes and Proteins with Reverse Network Engineering”, Project 1: Hilton-Ludwig Metastasis Initiative Scholar, Ludwig Institute for Cancer Research, Grant CRA-2431.9- 0-CB-3838, New York, NY, USA, Sole Principal Investigator (83,000 €), 2009-2012

• Development of the CONRAD metastatic gene/ protein and text-mining database” Project 2: Ludwig Institute for Cancer Research, UCL Branch London W1W 7BS, UK, CRA-2431.9-0-CB-3838, Project 1. Co- PI, 2008-2009

• Systems-level Analysis of Transcription in Immobilization Stress: Collaboration with Dr. Esther L. Sabban, Professor, Department of Biochemistry and Molecular Biology, New York Medical College, Valhalla, New York, USA, R01 NS044218/NS/NINDS, Co-Investigator, 2009-2011

• A Network Approach to Cyclin A1 Oncogenesis-Molecular analysis of differentiation mechanisms in acute promyelocytic leukemia (APL)” Principal Investigator, 2005-2007 P a p a n i k o l a o u C V

4

• Lung Epithelial-Mesenchymal Interactions in Adenocarcinoma Invasion, PT-AAAE3622 NIH, Co-PI, 2005- 2007 (500.000 $)

• Identification of metastasis-related proteins p40 and p35 in an ING1 tumor suppressor-associated, mSin3A nuclear complex, Ruth Kirschtein Fellow National Research Service Award, T32 CA09503-17 NIH, 2002-2003 (40,000 $)

• Isolation by MDM2-specific protein affinity chromatography of protein p110b from HDAC1/HeLa cell extracts and structure/function analysis, Ruth Kirschtein Fellow National Research Service Award, T32 CA09503-16 NCI, 2000-2001 and regulation of MDM2 Function by ZNF217/p110b, T32 CA09503-17 NCI, Ruth Kirschtein Fellow National Research Service Award, 2001-2002 (38,000 $)

• Characterization of the Human β3-Adrenoreceptor Gene. Program: Receptors with Seven-Membrane Spanning Regions, PIs: Emir Duzic and Tom Claus, Department of Cardiovascular Molecular Biology, American Cyanamid Company, Co-Investigator, 1994-1995

• Identification of a Second Egr-1 Response Element within the ApoAI Gene”. Program: Atherosclerosis/Liver-specific expression of the human Apolipoprotein AI gene, PIs: Edward Kilbourne and Sotirios K. Karathanasis, Department of Cardiovascular Molecular Biology, American Cyanamid Company, Co-Investigator, 1993-1994

• Construction of a Human Apolipoprotein A (ApoA) Gene Promoter Luciferase Reporter”. Program: Promoter constructs for drug screening, PIs: Emir Duzic and Sotirios K. Karathanasis, Department of Cardiovascular Molecular Biology, American Cyanamid Company, Molecular Biology Staff, 1992-1993

• Preparation, purification and quality control analysis of vitamin and glutathione mixtures. Formulation, analysis and quality control of glutathione-based formulations. Scale-up development of preparations. Contributed to phase-I preclinical studies of pharmaceutical preparations by compiling, analyzing and preparing pharmacological data for submission to the FDA, Thyone Corporation (Previously Health Maintenance Programs), Formulations Chemist, 1987-1992 Pre-/Clinical Trials

Phase-I preclinical studies of glutathione pharmaceutical preparations for HIV studies. Compiled, analyzed and prepared pharmacological data for submission to the FDA, Thyone Corporation (Previously Health Maintenance Programs), Formulations Chemist, 1987-1992 Web Tools/Databases

A neural network database of protein Arginine/Lysine methylation: Collaboration with Professor G. Amoutzias, University of Thessaly, Greece. The tool is freely available at: http://bioinf.bio.uth.gr/methyl- prometheus 2018-2020

Patents

Title: “A remote method of detection, evaluation and the management of human metabolic function through phenotypic intervention, using algorithms that deconvolute metabolic homeostasis in depth”, Status: Approved, expecting registration code.

P a p a n i k o l a o u C V

5

Publications (74 total)

In Preparation/ Submitted

Papanikolaou et al. Protein Arginine and Lysine Methylation in the era of Proteomics and Bioinformatics Papanikolaou N.A. et al. Design principles of MAPK signaling networks using automaton and dynamical systems theories

Papanikolaou N.A., et al. The Ras-related protein RAB22A interacts with hypoxia-inducible factor 1-alpha (HIF- 1α) in MDA-MB-231 breast cancer cells in hypoxia, 2023, Submitted to Cellular Oncology Fliatoura V, Vlastaridis P, Doudoumi C,Mossialos D, Iliopoulos I, Papanikolaou N.A., Amoutzias GD, (2023), Meth-Phos-Prometheus: A webserver for the prediction of protein methylation and phosphorylation sites, their rheostats, clusters and higher order combinatorial switches., Bioinformatics, In Press Books, Book Chapters & Monographs

Papanikolaou, N.A. “Oncogenes and Tumor Suppressor Genes: Mechanisms, in Biology of Cancer”, Chapter 6, pp. 67-73 (Hellenic Academy of Oncology, EAKO), 2007 Papanikolaou, N.A. “mTOR, Proteasome and PARP1, in Biology of Cancer”, Chapter 15, pp. 177-183,

(Hellenic Academy of Oncology, EAKO), 2007

Sabban, E. L. Nankova, B. B. Serova, L. I. Kvetnansky, R.; Papanikolaou, N. A. Nakashima, A. (2002).

“Dynamics of Transcriptional events in Stress-triggered Regulation of Adrenal Catecholamine Biosynthetic Enzymes, Catecholamine Research: From Molecular Insights to Clinical Medicine”

(Advances in Behavioral Biology) -US-

ISBN: 978********** (Hard cover book) (Editor: Kluwer/Plenum Academic Publishers), Vol 53, pages 321-324

Marks’ Medical Biochemistry, 2104 Edition, Laboratory of Biological Chemistry, Department of Medicine, Aristotle University of Thessaloniki, Macedonia, Greece: Multi-author translation in Greek, 2014 Triantafyllidis K. “The Genetic Origins of the Greeks” Kyriakidis Publications, 2018, Role: EDITOR, In Greek language, lay person’s publication

Triantafyllidis K. “The Genetic Origins of the Greeks” Kyriakidis Publications, 2019, Role: EDITOR, Technical presentation of the Genetics of the Greeks

Refereed Journals

Publications in: https://www.med.auth.gr/en/user/877/publications Ladias C, Papakotoulas P, Papaioannou M, Papanikolaou NA. Overcoming phenotypic switching: targeting protein-protein interactions in cancer. Explor Target Antitumor Ther. 2023;4:1071–81. https://doi.org/10.37349/etat.2023.

P a p a n i k o l a o u C V

6

Papanikolaou NA, Nikolaidis M, Amoutzias GD, Fouza A, Papaioannou M, Pandey A, Papavassiliou AG. The Dynamic and Crucial Role of the Arginine Methylproteome in Myoblast Cell Differentiation. Int J Mol Sci. 2023 Jan 20;24(3):2124. Doi: 10.3390/ijms24032124. PMID: 36768448; PMCID: PMC9916730. Papanikolaou NA, Hytiroglou P, Pantelidou P, Papavassiliou AG and Old LL (2022), Systems-Level Mapping of Cancer Testis Antigen 1b/a to Sarcoma Pathways Identifies Activated Ran Binding-2 E3 SUMO- Protein Ligase and Transducin-Like Enhancer Protein 1. Front. Genet. 13:834445. PMID: 35664317 PMCID: PMC9158115 DOI: 10.3389/fgene.2022.834445

Karakota M, Gounari E, Koliakou I, Papaioannou M, Papanikolaou NA, Koliakos G. Induced differentiation and molecular characterization of monocytes-derived multipotential cells generated from commonly discarded leukapheresis filters. Tissue Cell. 2022 May 20;77:101825. Doi: 10.1016/j.tice.2022.101825. Epub ahead of print. PMID: 35679687.

Tsakona D, Galliou PA, Papanikolaou NA (2019) Identification with SILAC Proteomics of Novel Short Linear Motifs in Demethylase Enzymes Regulated During Myoblast Differentiation. Cell Dev Biol 7: 198. Doi: 10.4172/2168-9296.1000198.

Tsolkas, D. Komninou, E. Briasoulis, E. Hatzimichael and N.A. Papanikolaou A Novel Therapeutic Approach in Acute Promyelocytic Leukemia with All-trans retinoic Acid and Cyclin-dependent Kinase Inhibitors. Clinical Cancer Drugs (2018) 5: 50. https://doi.org/10.2174/2212697X05666180713164713 Tsolkas G, Komninou D and Papanikolaou N.A. Differentiation of acute promyelocytic leukemia cells by all- trans retinoic acid and a cyclin-dependent kinase inhibitor involves dissociation of a CDK4/C/EBPε complex”, 2018. J Biochem Cell Biol 1: 103. DOI: 10.4172/jbcb.1000103 Tsolkas G, Komninou D, Papanikolaou NA (2018) Cyclin A1 Expression is Reciprocally Controlled by the Transcription Factor ZNF217and miRNAs in Invasive Breast and Prostate Cancer Cells: An In Silico Analysis. J Biochem Cell Biol 1: 104

Nikolaos I. Kalosidis, Aegli Mantsou and Nikolaos A. Papanikolaou. From driver mutations to driver protein cancer networks: Why we need a new paradigm”, Cancer Studies, 2018; 2(1):1 (Invited manuscript). DOI:10.2174/2212697X05666180713164713

Mantsou A, Koutsogiannouli E, Costas Haitoglou, Papavassiliou AG and Papanikolaou NA Regulation of Expression of the p21CIP gene by the Transcription Factor ZNF217 and MDM2”. Biochemistry and Cell Biology, 2016, 94(6): 560-568, doi.org/10.1139/bcb-2016-0026 Papanikolaou NA, Tillinger A, Liu X., and Sabban, E. A Systems Approach identifies co-Signaling Molecules of Early Growth Response Transcription Factor in Immobilization Stress”. (2014. BMC Systems Biology 2014, 8:100. doi.org/10.1186/s12918-014-0100-8

Valentinos Kounnis, Georgios Chondrogiannis, ichalis D. Mantzaris, Nikolaos A. Papanikolaou, et al. Microcystin LR is a new candidate molecule for the development of novel therapeutics against P a p a n i k o l a o u C V

7

pancreatic cancer specifically expressing the OATP1B3 transporter. Anticancer Research, November 2015 vol. 35 no. 11 5857-5865.

Koutsogiannouli, E., Papavassiliou, A. G. and Papanikolaou, N. A. (2013), Complexity in Cancer Biology: Is Systems Biology the Answer? Cancer Medicine 2013; 2(2): 164–177. https://doi.org/10.1002/cam4.6 Ziouti F, Gbandi E, and Papanikolaou NA, Chromatin and Eukaryotic Gene Silencing-Part II”. Mediterranean Oncology Journal, 2012: 34-38.

Gbandi E, Ziouti F and Papanikolaou NA, Rb Chromatin and Gene Silencing – Part Ι”. Mediterranean Oncology Journal 2012, 1:30-34.

Ntemka A, Iliadis F, Papanikolaou NA and Grekas D. A Network-Centric Analysis of Genetic Predisposition in Diabetic nephropathy. Hippokratia 2011, 15, 3: 232-237. https://www.ncbi.nlm.nih.gov/pubmed/22435020

Papanikolaou NA, Mechanism-based Rational Targeting in Acute promyelocytic Leukemia. In Vivo. 2010, 24: 21-28 https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3306029/ Papanikolaou NA, Papavassiliou AG. Protein Complex, Gene, and Regulatory Modules in Cancer Heterogeneity. Mol Med. 2008 Jul 25, 14(9-10): 543–545. Published online 2008 July 25. Doi: 10.2119/2008-00083 PMID: 18654660.

Borczuk AC, Papanikolaou N, Toonkel RL, Sole M, Gorenstein LA, Ginsburg ME, Sonett JR, Friedman RA, Powell CA. Lung adenocarcinoma invasion in TGFbetaRII-deficient cells is mediated by CCL5/RANTES. Oncogene. 2008 Jan 17;27(4):557-64. https://www.nature.com/articles/1210662 Nikolaev, A., Papanikolaou, N. A, Li, M. Qin, J. and Gu W. (2004). Identification of a novel breast metastasis-1 (BRMS1)-homologue protein p40 as a component of the mSin3A/p33ING1b/HDAC1 deacetylase complex. Bioch. Biophys. Res. Commun. 323, 1216-1222. https://doi.org/10.1016/j.bbrc.2004.08.227

Papanikolaou, N.A. Vasilescu ER, and N. Suciu-Foca (2004). Novel Single Nucleotide Polymorphisms in the Human Immune Inhibitory Immunoglobulin-like T cell Receptor type 4 (ILT4). Human Immunology, 65, 700-705. https://doi.org/10.1016/j.humimm.2004.04.003 Papanikolaou, N.A. Histone Deacetylase Complexes in Neoplasias (Review in Greek, English Abstract), Forum of Clinical Oncology Vol 2(B) No3/4, 239-248 (2003). El-Bayoumy K, Rose D.P., Papanikolaou N, Leszczynska J, Swamy M.V., Rao C.V., “Cyclooxygenase-2 expression Influences the Growth of Human Large and Small Cell Lung Carcinoma Lines in Athymic Mice: Impact of an Organoselenium Compound on Growth Regulation,” Int. J. of Oncol., Vol 20, No 3, pp. 557-561, 2002. https://doi.org/10.3892/ijo.20.3.557 P a p a n i k o l a o u C V

8

Papanikolaou N.A., and Sabban, E.L., Ability of Egr1 to activate tyrosine hydroxylase transcription in PC12 Cells: Cross-Talk with AP-1 Factors. J. Biol. Chem. Vol. 275, pp. 266**-*****, 2000. https://doi.org/10.1016/S0021-9258(19)61430-4

Papanikolaou, N.A., and Sabban, E.L. Sp1/Egr1 Motif: A New Candidate in the Regulation of Rat Tyrosine Hydroxylase Transcription by Immobilization Stress. J. Neurochem, Vol. 73, pp. 433-436, 1999. https://doi.org/10.1046/j.1471-4159.1999.0730433.x Chang-Xi Zu, Roche, C. J., Papanicolaou, N.A., DiPietrantonio, A, and Yuk-Ching Tse-Dinh. Site-Directed Mutagenesis of Conserved Aspartates, Glutamates and Arginines in the Active Site of Escherichia coli DNA Topoisomerase I. J. Biol. Chem. Vol. 273, pp. 8783-8789, 1998. https://doi.org/10.1074/jbc.273.15.8783

Hsieh, T.C., Aguero-Rosenfeld, M.E.,Wu, J.M, Ng, C., Papanikolaou, N.A., Varde, S.A., Schwartz, I., Pizzolo, J.G., Melamed, M., Horowitz, H.W., Nadelman, R.B., and Wormser, G.P. Cellular Changes and Induction of Apoptosis in Human Promyelocytic HL-60 Cells Infected with the Agent of Human Granulocytic Erlichiosis (HE). Biochemical and Biophysical. Research Communications Vol. 232, pp. 298-303, 1997. https://doi.org/10.1006/bbrc.1997.6276

Refereed Conference Proceedings

M. Xatziathanasiou, D. Kiouri, C. Hasapis, H. Dallis, E. Drosopoulou, and N. Papanikolaou, Protein docking and molecular dynamics analysis of the effect of mutation D816V on the binding of imatinib on oncoprotein c-KIT, 12th Symposium of the School of Medicine, Aristotle University of Thessaloniki, 15/3/2023-17/3/2023.

Galliou, P. A., Verrou, K. M., Papaioannou, M., Papanikolaou, N. A., Koliakos, G., Phosphorylation mapping of laminin γ1-chain: Kinases in association with active sites and cancer mutations. 70th Hellenic Society for Biochemistry and Molecular Biology Annual Meeting, Athens, Greece, 70th Symposium of the Hellenic Society for Biochemistry & Molecular Biology, Evgenides Institute, 29/11/2019-01/12/2019.

Galliou, P. A., Verrou, K. M., Papaioannou, M., Papanikolaou, N. A., Koliakos, G., Phosphorylation mapping of laminin γ1-chain: Kinases in association with active sites and cancer mutations. 7th Panhellenic Forum of Young Scientists, 7ο Panhellenic Forum of Young Scientists of the Hellenic Society for Biochemistry and Molecular Biology, Evgenides Institute, Athens Greece, 28/11/2019.

Tsakona D., Galliou P.A., Papanikolaou N.A. (2019) Bioinformatics inference of a novel functional link between PKA and the transcription factor ZNF217. Hellenic Bioinformatics, 12th International Conference, Heraklion, Crete, Greece.

Tsakona D., Galliou P.A., Papanikolaou N.A. (2019) Identification of intrinsically disordered regions and short linear motifs in the human transcription factor ZNF217. Hellenic Society for Biological Sciences, 41thAnnual Conference, May 2019, Katerini, Greece. P a p a n i k o l a o u C V

9

Tsakona D, Galliou P.A. and Papanikolaou N.A., 2019. Enrichment of Deleterious mutations in intrinsically disordered regions (IDRs) and short linear motifs (SLiMs) in the human transcription factor ZNF217May 2019Conference: 41st Conference of Hellenic Society for Biological Sciences, Katerini, Greece Tsakona D, Galliou PA, Papanikolaou NA (2018) Identification of Short Linear Motifs In Demethylase Enzymes Regulated During Myoblast Differentiation With SILAC Proteomics, 8th Scientific Conference, School of Medicine, Abstract 14-16 March, 2019, Thessaloniki, Greece. Tsakona D., Papanikolaou N.A. (2018) Analysis of intrinsically disordered regions of human transcription factor ZNF217. Hellenic Society for Biochemistry and Molecular Biology, 69th Annual Conference, November 2018, Larissa, Greece.

Mantsou Α., Papanikolaou N. The E3 ubiquitin ligase MDM2 is a target of methylation on lysines, 38th Symposium, Hellenic Society for Biological Sciences, Abstract 26-28 May, 2016, Kavala, Greece Nikolaos A. Papanikolaou, Min-Sik Kim,Sartaj Ahmad Mir, Raiha Tahir and Akhilesh Pandey, Profiling Arginine methylation during myoblast differentiation using SILAC, Hellenic Society for Biochemistry and Molecular Biology 66th Annual Conference, December 2015 Kounnis V, G. Chondrogiannis, M.D. Mantzaris, D. Fokas, N.A. Papanikolaou, I. Sainis, E. Briasoulis et al. Pancreatic Cancer Cells Expressing the OATP1B3 Transporter show promising senisitivity to the highly cytotoxic microcystin-LR molecule. European Journal of Cancer Volume 50, Supplement 6, November 2014, Pages 86, 26th EORTC – NCI – AACR Symposium on Molecular Targets and Cancer Therapeutics Papanikolaou Nikolaos A, Koutsogiannouli Evangelia, Papavassiliou Athanasios, Functional Interactions between Mouse Double Minute 2 Protein and a putative oxidase, Hellenic Society for Biochemistry and Molecular Biology 64th Annual Conference, December 2013 Koutsogiannouli E, Papavassiliou A and Papanikolaou N.A. Isolation and Characterization of novel protein p110b in a CoREST/HDAC1/2 complex, Chemistry Conference, September 2011 Koutsogiannouli E, Papavassiliou AG. Gu W. and Papanikolaou N.A. Regulation of p53 Function by a p110b/MDM2/HDAC Complex. The Hellemic Society for Biochemistry and Molecular Biology, December 2011

Koutsogiannouli E, Haitoglou K, Papageorgiou G and Papanikolaou N.A. Oncogenic Mechanisms of a p110b Multiprotein Complex. Hellenic Medical Society Conference, September 2011 Triantafillou Ε, Tzimagiorgis G, Haitoglou K and Papanikolaou N.A. Correlation between Cyclin A expression and miRNA-335 in breast and prostate lung cancer cell lines. ESFIE Conference, 2012 Constantinidou S and Papanikolaou N. A. Ιn silico analysis of the secondary structure of testis cancer protein MAGEC1, Conference of the Medical School, Aristotle University of Thessaloniki, 2011 Tsolkas G. Vavatsi-Christaki N. Kosmopoulou I. Papanikolaou N.A. Synergistic induction of differentiation in acute promyelocytic leukemia cells by low levels of all-trans Retinoic acid and CDK inhibitors. Conference: The Hellenic Society for Biochemistry and Molecular Biology, December 2011 P a p a n i k o l a o u C V

10

Tsolkas G, Koutsogiannouli EA, Tzimagiorgis G, Haitoglou K, Xytiroglou M and Papanikolaou N.A, miRNA- mediated regulation of cyclin A1 expression, Conference: The Hellenic Society for Biochemistry and Molecular Biology, December 2011

Tsolkas G and Papanikolaou N. A. A Study on the Role of Retinoic Acid and Cyclin-dependent Kinase Inhibitors on the Differentiation of Acute Promyelocytic Leukemia, Hellenic Medical Society, Thessaloniki, 2011

Andrej Tillinger, Xiaoping Liu, Nikolaos A Papanikolaou, Lidia I Serova, Esther L Sabban. Analysis of Signaling Pathways Triggering Transcriptional Changes in Adrenal Medulla with Single and Repeated Stress. Experimental Biology 2009 (Biochemistry/Molecular Biology, ASBMB), ABSTRACT ID: 5197 Papanikolaou N.A. “Rational Targeting of Acute Promyelocytic Leukemia with combined ATRA and CDK inhibitors” 2005, 18th annual Gene Center Symposium on Chemical Biology: Intervention in Cells based on Chemical Principles, Hunter College Chemistry Department, Friday January 14th, 2005 Papanikolaou, N. A., Toοnkel E., Sole, M., Lachman, S., and Powell, C.A. “Invasion of human lung Adenocarcinoma Induced by TGFβR2 Suppression may be Mediated by Upregulation of RANTES”. Abstract, Keystone Symposia on Quantitative Biology 2007 Flavia Piazza, Nikolaos Papanikolaou, Luigi Scotto, John Manavalan, Raffaello Cortesini, and Nicole Suciu- Foca. “Signaling properties of inhibitory receptors ILT3 and ILT4 in tolerogenic dendritic cells”. Abstract, American Association of Immunology 2004 Annual Meeting, Experimental Biology (EB 2004), Washington, D.C., April 17-21, 2004

Sabban E.L., Nankova B.B., Serova L.I., and Papanikolaou N.A., “Different transcriptional mechanisms activating gene expression of catecholamine biosynthetic enzymes depending on duration or repetition of stress,” FASEB J. vol. 14, No 8, pp. 685 May 11, 2000 Papanikolaou, N.A., and Sabban, E.L., “The Sp-1/Egr-1 motif of the tyrosine hydroxylase promoter may be involved in its transcriptional regulation by stress,” FASEB J., Vol. 13, No. 5, pp. A792-A793 Part 2 Suppl. Mar 15, 1999

Papanikolaou, N.A., and Sabban, E.L., “Sp1/Egr1: A New Functional Element in the Regulation of Tyrosine Hydroxylase Transcription” Experimental Biology, Vol. 618.4, pp.230, 1999 Sabban, E.L, Nankova, B., Serova, L., Kvetnansky, R., and Papanikolaou, N. “Multiple Signaling Pathways in the Molecular Regulation of the Catecholamine Biosynthetic Enzymes by Stress,” Chromaffin Cell Biology. Bergen, Norway, 1999

Papanikolaou, N.A., and Sabban, E.L., “Transcriptional Mechanisms in Stress-elicited Activation of Tyrosine Hydroxylase Gene Expression: Role of AP-1 and SP-1 Motifs” in: Mechanisms of Transcription, Cold Spring Harbor Symposia for Quantitative Biology, Abstract, June 3-8, 1998 Papanikolaou, N.A., and Sabban, E.L., “Mapping of Tyrosine Hydroxylase and Dopamine Hydroxylase Enhancer Elements Involved in Immobilization Stress by DNAse-I Footprinting” 11nth Annual Graduate Forum, New York Medical College, Abstract, June 1998 P a p a n i k o l a o u C V

11

Papanikolaou, N.A., and Sabban, E.L., Promoter Elements involved in the Transcriptional Regulation of Rat Tyrosine Hydroxylase by Immobilization Stress. Society for Nueroscience, Abstract, Washington, DC. 1998

Papanikolaou, N.A., and Sabban, E.L., “Inhibition of Nuclear Protein Binding to the AP-1 Motif in the Tyrosine Hydroxylase Promoter by a Triplex-forming Oligonucleotide” 10nth Annual Graduate Forum Abstract, New York Medical College, May 199

Papanikolaou, N.A., Nankova, B., Sabban, E. L., “Triplex Forming Oligonucleotide to the AP-1 Site on the Rat Tyrosine Hydroxylase Promoter,” Society for Neuroscience, Vol. 235.12, p120, 1996 Papanikolaou, N.A., and Tse Dinh, Y.C., “Effect of Site-Directed Mutagenesis on E. Coli Topoisomerase-I Enzymatic Activity” 9nth Annual Graduate Forum Abstract, New York Medical College, May 1996 Duzic, E., Claus, Tom. & Papanikolaou, N.A. Characterization of the human β3-Adrenoreceptor and glucagon genes and their receptors, Lederle Labs Publications, 1994 Kilbourne, E, J., Papanikolaou, N.A., and Karathanasis, S. K. Identification of a Second Egr-1 Response Element within the ApoAI Gene, Lederle Labs Publications, 1993 Duzic, E., Papanikolaou N.A., & Karathanasis S. K. Construction of a Human Apolipoprotein [Apo (A1)] Gene Promoter Luciferase Reporters, Lederle Labs Publications, 1993 Harnish, D.C., Ferris, E., Potla, L., Papanikolaou, N.A., & Karathanasis, S. “Construction and Validation of

Contact this candidate