Xiuyun Hou

*** ***** ******, **********, ** *****

ad1ntg@r.postjobfree.com 781-***-****

Visa Status: Citizen

SUMMARY

A highly motivated Senior Lab manager with twenty plus+ years experience in the academic and industry environment. As Lab Manager, I oversee the administration and workflow of Hammond Labortory, known for a combination of finely tuned scientific, management, and people skills. Provides the highest-quality support to preclinical drug discovery through expertise in cellular and molecular biology.

Excellent research-problem solving and data management skills for being highly organized, reliable and keenly attentive to detail.

Great communication and management skills for writing the research, biosafety, and animal protocols; leading lab tours and training lab members; working with EHS for the lab safety; working with vendors for lab supplies; and working with finacial officers for lab budgets.

Be passionate about six years volunteer of Koch CCL for organizing the Friday Focus, also volunteer as a tutor for MIT HR ESL program.

PROFESSIONAL EXPERIENCE

Senior Lab Manager, Koch Institute for Integrative Cancer Research, Massachusetts Institute of Technology, (05/2016- present) PI: Paula Hammond

Cardiovascular Physiology Core manager and lab manager, Cardiac Muscle Research Lab, Genetics Division, Brigham and Women’s Hospital, (05/2015- 04/2016) PI: Ronglih Liao

Lab Manager, Renal Division, Brigham and Women’s Hospital, (02/2014- 04/2015) PI: Ben Humphreys

Research Technologist, Vascular Biology Section, Boston University School of Medicine, (01/2005-01//2014) PI: Richard A. Cohen

Senior Research Assistant, Vascular Biology Section, Boston University School of Medicine, (10/01-12/04) PI: Minghui Zou and Richard A. Cohen

Scientific experience

ECHO( Vevo 2100) images( B mode, M mode, Color Doppler) on mouse( neonatal and adult), rat, and zebrafish( larva and adult)

Write, renew and amend biosafety, animal protocols, PIBC and coordinate with EHS, animal facility staff to ensure that biosafety, IACUC animal protocols are followed.

Zebrafish, mouse colony management, breeding strategies, animal models and analysis of animal tissues.

Design, perform, and analyze research experiments.

Present results for discussion, publication, and grant proposal.

Executed independent and group research projects within the lab.

Coordinated collaborative projects with other university laboratories and pharmaceutical

Companies resulting in peer journal publications.

Cell culture, cell isolation, cell-based assays, and cell transfection and infection, and flow cytometry(FACS)

ELISA, SDS-PAGE, Western blot, immunoprecipitation, DNA isolation, plasmid preparation, RNA extraction, RT-PCR, real time-qPCR, and protein over expression and purification.

Chromatographic techniques: HPLC; Spectroscopic analysis: UV, IR, NMR, and MS.

Management experience

Safety management, acting as EHS representative and managing and disposing of chemical, biological, and radioactive wastes; managing lab supplies; managing animal facility including handling animal protocol, renewals and amendments; managing TC culture operation of a lab with 35 personnel.

Managed the Core facility.

Oversee the administration of a laboratory with12 personnel, managed zebrafish, mouse colony with 30 lines, around 300 cages.

Managed cell culture operation of a laboratory with 30 personnel.

Responsibilities include ordering supplies, inactive FBS, maintain frozen cells, maintenance of the incubators, CO2 and Liquid nitrogen tanks.

Supervise and train new faculty, post doctoral associates, staff and students regarding cell culture room policies and procedures.

Contractor (part-time), Molecular Biology Department, Ensemble Therapeutics, Cambridge, MA

(09/09-12/09)

Consultant for cell culture

Research Assistant, Chemistry Department, Natural Pharmaceutical International, Inc., Burlington, MA (05/01-10/01)

· Isolation of Boswellic acids from Boswellia carterrii for bioassay against prostate cancer

· Isolation of silybin from Silybum marianum for treatment of liver damage

Research Associate & Engineer, Department of Natural Products Chemistry, Liaoning Institute of Materia Medica, China (08/89-08-95, 08/98-07/00)

Extraction, isolation, identification and structural elucidation of active constituents from natural resources

Expertise in purification and characterization of natural and synthetic products using standard chromatography (HPLC, TLC, and various columns) and spectroscopic analysis (UV, IR, 1D & 2D NMR, MS)

Development of new analytical HPLC method

EDUCATION

Shenyang Pharmaceutical University, China, M.S., Pharmaceutical Chemistry, 1998.

Liaoning College of Chinese Traditional Medicine, China, B.S., Chinese Traditional Medicine, 1989.

TECHNIQUES AND SKILLS

Animal studies

1)Intraperitoneal, intravenous, Intramuscular, Intranasal, Intranodal, Intradermal, Retro-Orbital and subcutaneous injection.

2)Oral gavage, organ dissection and blood collection.

3)Type 2 diabetic and atherosclerotic animal models, analysis of glucose and lipid profile in serum and liver of mice and analysis of triglycerides and cholesterol in cells

Cell biology

1)Mammalian cell culture (both primary culture and cell line culture), managing cell culture room and ordering supplies.

2)Cell isolation (rat cardiac myocyte, human vein smooth muscle cells and mouse heart endothelial cells).

3)Cell-based assays: cell permeability, cell migration, proliferation, amplex red assay for ROS production, immunohistochemical staining on animal heart, aorta, muscle, kidney, liver, neck, skin, pancreas, cells and human tissue (taking pictures, making files and using Image J to do analysis), and flow cytometry (FACS) assay.

4)Cell transfection (siRNA, shRNA, DNA plasmid) and viral infection.

Molecular biology and biochemistry

1)ELISA, SDS-PAGE, Western blot, immunoprecipitation.

2)Genomic DNA isolation, plasmid preparation, sub-clones.

3)ChIP, RNA extraction, RT-PCR, real time-qPCR, Northern blots, in situ-hybridization.

4)Protein over expression and purification for MALDI-TOF Mass Spectrometry Analysis.

5)Enzyme activity assays.

Computer Skills

Microsoft Word, Excel, Photoshop, PowerPoint, Prism and ANOVA

PUBLICATIONS

1.Tong X, Hou X, Wason C, Kopel T, Cohen RA, Dember LM. Smooth Muscle Nitric Oxide Responsiveness and Clinical Maturation of Hemodialysis Arteriovenous Fistulae. The American Journal of Pathology, 2017 Sept 187

2.Shao D, Han J, Hou X, Fry JL, Behring JB, Seta F, Long M, Roy H, Cohen RA, Matsui R, Bachschmid MM. Glutaredoxin-1 deficiency causes fatty liver and dyslipidemia by inhibiting sirtuin-1. Antioxid Redox Signal. 2016 Dec 13.

3.Maarouf OH, Aravamudhan A, Rangarajan D, Kusaba T, Zhang V, Welborn J, Gauvin D, Hou X, Kramann R, Humphreys BD. Paracrine Wnt1 Drives Interstitial Fibrosis without Inflammation by Tubulointerstitial Cross-Talk. Journal of the American Society of Nephrology. 2015 Jul 23 ASN 201421188

4.Chen L, Hou X, Xiao J, Kuroda J, Ago T, Sadoshima J, Cohen RA, Tong X. Both hydrogen peroxide and transforming growth factor beta 1 contribute to endothelial Nox4 mediated angiogenesis in endothelial Nox4 transgenic mouse lines. Biochimica et Biophysica Acta (BBA) 2014, 1842, 2489-2499

5.Qin Z, Hou X, Weisbrod RM, Seta F, Cohen RA, Tong X. Nox2 mediates high fat high sucrose diet-induced nitric oxide dysfunction and inflammation in aortic smooth muscle cells. Journal of Molecular and Cellular Cardiology 2014, 72, 56-63

6.Shao D, Fry JL, Han J, Hou X, Pimentel DR, Matsui R, Cohen RA, Bachschmid MM. A redox-resistant sirtuin-1 mutant protects against hepatic metabolic and oxidant stress. Journal of Biological Chemistry 2014, 289(11), 7293-306

7.Hobai IA, Buys ES, Morse JC, Edgecomb J, Weiss E, Armoundas AA, Hou X, Khandelwal AR, Siwik DA, Brouckaert P, Cohen RA, Colucci WS. SERCA cysteine-674 sulphonylation and inhibition of L-type calcium influx contribute to cardiac dysfunction in endotoxemic mice, independently of cGMP synthesis. American Journal of Physiology - Heart and Circulatory Physiology 2013, 305(8), H1189-200

8.Haeussler DJ, Primentel DR, Hou X, Burgoyne JR, Cohen RA, Bachschmid MM. Endomembrane H-Ras controls vascular endothelial growth factor-induced nitric oxide synthase-mediated endothelial cell migration. Journal of Biological Chemistry 2013, 288(21), 15380-9

9.Verbeuren, T.J., Xu, S., Hou, X., Bachschmid, M.M., Jiang, B., Li, Y., Zang, M., Rupin, A., and Cohen, R.A. New Aspects of Vascular Actions of Polyphenols. Science MED 2012, 3, 69-74

10.Qin F, Siwik DA, Luptak I, Hou X, Wang L, Higuchi A, Weisbrod RM, Ouchi N, Tu VH, Calamaras TD, Miller EJ, Verbeuren TJ, Walsh K, Cohen RA, Colucci WS. The Polyphenols Resveratrol and S17834 prevent the Structural and Functional Sequelae of Diet-Induced Metabolic Heart Disease In Mice. Circulation 2012, 125(14), 1757-64

11.Xu S, Zhi H, Hou X, Jiang B. Angiotensin II modulates interleukin-1β-induced inflammatory gene expression in vascular smooth muscle cells via interfering with ERK-NF-кB crosstalk. Biochemical and Biophysical Research Communications 2011, 410(3), 543-8

12.Xu S, Zhi H, Hou X, Cohen RA, Jiang B. I{kappa}B{beta} Attenuates Angiotensin II-Induced Cardiovascular Inflammation and Fibrosis in Mice. Hypertension 2011, 58(2), 310-6

13.Xu S, Jiang B, Hou X, Shi C, Bachschmid M, Zang M, Verbeuren T J, Cohen RA. High Fat Diet Increases and the Polyphenol, S17834, Decreases Acetylation of the SirT1-Dependent Lysine-382 on p53 and Apototic Signaling in Atherosclerotic Lesion-Prone Aortic Endothelium of Normal Mice. Journal of Cardiovascular pharmacology 2011, 58(3), 263-71

14.Li Y, Xu S, Mihaylova MM, Zheng B, Hou X, Jiang B, Park O, Luo Z, Lefai E,Shyy JY, Gao B, Wierzbicki M, Verbeuren TJ, Shaw RJ, Cohen RA, Zang M. AMPK phosphorylates and inhibits SREBP activity to attenuate hepatic steatosis and atherosclerosis in diet-induced insulin-resistant mice. Cell Metabolism 2011, 13, 376-388

15.Li Y, Xu S, Giles A, Nakamura K, Lee JW. Hou X, Donmez Gizem, Li J, Luo Z, Walsh K, Guarente L, Zang M. Hepatic overexpression of Sirt1 in mice attenuates endoplasmic reticulum stress and insulin resistance in the liver. FASEB J. 2011, 25, 1-16

16.Tong X, Hou X, Jourd’heuil D, Weisbrod RM, Cohen RA. Upregulation of Nox4 by TGF{beta} 1 oxidizes SERCA and inhibits NO in arterial smooth muscle of the prediabetic Zucker rat. Circulation Research 2010, 107, 975-983

17.Zee RS, Yoo CB, Primentel DR, Perlman DH, Burgoyne JR, Hou X, McComb ME, Costello CE, Cohen RA. Redox Regulation of Sirtuin-1 by S-Glutathiolation. Antioxidants & Redox Signaling 2010, 13, 1023-1032.

18.Tang WH, Kravtsov GM, Sauert M, Tong X, Hou X, Wong T, Chung S, Chung S. Polyol pathway impairs the function of SERCA and RyR in ischemic-reperfused rat hearts by increasing oxidative modifications of these proteins. Journal of Molecular and Cellular Cardiology 2010, 49 58-69.

19.Tang WH, Cheng WT, Kravtsov GM, Tong X, Hou X, Chung S, Chung S. Cardiac Contractile Dysfunction during Acute Hyperglycemia Due to Impairment of SERCA by Polyol Pathway Mediated Oxidative Stress. American Journal of Physiology- Cell Physiology 2010, 299, C643-C653.

20.Hou X, Xu S, Maitland-Toolan KA, Sato K, Jiang B, Ido Y, Lan F, Walsh K, Wierzbicki M, Verbeuren TJ, Cohen RA, and Zang M. SIRT1 regulates hepatocyte lipid metabolism through activating AMP-activated protein kinase. Journal of Biological Chemistry 2008, 283: 200**-*****.

21.Zang M, Xu S, Maitland-Toolan KA, Zuccollo A, Hou X, Jiang B, Wierzbicki M, Verbeuren TJ, and Cohen RA. Polyphenols Stimulate AMP-Activated Protein Kinase, Lower Lipids, and Inhibit Accelerated Atherosclerosis in Diabetic LDL Receptor–Deficient Mice. Diabetes 2006, 55, 2180-2191.

22.Clavreul N, Bachschmid MM, Hou X, Shi C, Idrizovic A, Ido Y, Pimental D, Cohen RA. S-Glutathiolation of p21ras by Peroxynitrite Mediates Endothelial Insulin Resistance Caused by Oxidized Low-Density Lipoprotein. Arteriosclerosis, Thrombosis and Vascular Biology 2006, 26, 2454-61.

23.Xu S, Bayat H, Hou X, and Jiang B. Ribosomal S6 kinase-1 modulates interleukin-1β induced persistent activation of NF-κB through phosphorylation of I-κBβ. American Journal Physiology Cell Physiology 2006, 291, C1336-45.

24.Zang M, Zuccollo A, Hou X, Nagata D, Walsh K, Herscovitz H, Brecher P, Ruderman NB, Cohen RA. AMP-activated Protein Kinase is Required for the Lipid-lowering Effect of Metformin in Insulin-resistant Human HepG2 Cells. Journal of Biological Chemistry 2004, 279, 478**-*****.

25.Adachi T, Pimentel DR, Heibeck T, Hou X, Lee YJ, Jiang B, Ido Y, Cohen RA. S-glutathiolation of Ras mediates redox-sensitive signaling by angiotensin II in vascular smooth muscle cells. Journal of Biological Chemistry 2004, 279, 298**-*****.

26.Jiang B, Xu S, Hou X, Pimentel DR, Cohen RA. Angiotensin II Differentially RegulatesInterleukin-1- -inducible NO Synthase (iNOS) and Vascular Cell Adhesion Molecule-1(VCAM-1) Expression: role of p38 MAPK. Journal of Biological Chemistry 2004, 279, 203**-*****.

27.Jiang B, Xu S, Hou X, Pimentel DR, Brecher P, Cohen RA. Temporal Control of NF- B Activation by ERK Differentially Regulates Interleukin-1 -induced Gene Expression. Journal of Biological Chemistry 2004, 279, 1323-1329.

28.Zou M, Hou X, Shi C, Kirkpatrick S, Liu F, Goldman MH, Cohen RA. Activation of 5'-AMP-activated Kinase Is Mediated through c-Src and Phosphoinositide 3-Kinase Activity during Hypoxia-Reoxygenation of Bovine Aortic Endothelial Cells: Role of Peroxynitrite. Journal of Biological Chemistry 2003, 278, 340**-*****.

29.Zou M, Hou X, Shi C, Nagata D, Walsh K, Cohen RA. Modulation by Peroxynitrite of Akt- and AMP-activated Kinase-dependent Ser1179 Phosphorylation of Endothelial Nitric Oxide Synthase. Journal of Biological Chemistry 2002, 277, 325**-*****.

30.Hou X, Chen F. Studies on Chemical Constituents of Lilium brownii. Acta Pharmaceutical Sinica 1998, 33, 923-926.

31.Hou X, Chen F, Wu L. New steroidal saponin of Lilium brownii. Chinese Journal of Medical Chemistry 1998, 8, 49, 53.

32.Hou X, Wo Y, Deng B. Determination of butyl flufenamate in combec ointment by UV method. China Journal of Mathematical Medicine 1998, 8, 22-26.

Contact this candidate