Curriculum vitae

Kahsay Huruy Ghezehegn, Ph.D.

Address: ***** **** ****** ***** ******* Way,Washington 98003, USA Phone: +1-360-***-****; E-Mail: ad0rme@r.postjobfree.com/ad0rme@r.postjobfree.com EDUCATION

Ph.D. degree in Medical Science (specialized in Medical Virology) 2015

Institute of Virology, Leipzig University, Germany Master of Science degree in Tropical & Infectious Diseases 2007

Addis Ababa University, Ethiopia, Aklilu Lemma Institute of Pathobiology Bachelor of Science, Medical Laboratory Technology 2003

Jimma University, Ethiopia, School of Medical Laboratory Sciences PROFESSIONAL EXPERIENCE

As CLS 2022-present

As clinical Lab Scientist at the University of Washington, Seattle at Micro Lab Founder of Halyet Health Care PLC, Ethiopia 2019-present

Researcher and senior laboratory technologist

Consulting clinical laboratories

Riwyet Health Services, PLC, Ethiopia 2016 – 2020

Senior medical laboratory technologist

Consultant for medical virology research team, and diagnostic techniques Ethiopian public health institute and University of Gondar, Ethiopia 2017-present

Writing scientific papers

Advising students

Reviewing scientific journals

Assistant professor and senior medical laboratory technologist, University of Gondar, Ethiopia 2015-2017 Principal investigator, Ethiopian Public Health Institute, Ethiopia 2015 – 2016 Lecturer and senior medical laboratory technologist, University of Gondar, Ethiopia 2007-2009 Assistant lecturer and medical laboratory technologist, University of Gondar, Ethiopia 2003-2005 TEACHING EXPERIENCE

UNIVERSITY OF GONDAR, ETHIOPIA

Medical Virology, Medical Microbiology, Advanced Diagnostic Microbiology, Bacteriology, Immunology, Medical Parasitology, Clinical Hematology, Clinical Chemistry, Immunohematology, and Urinalysis 2003-2016 RESEARCH EXPERIENCE

Molecular characterization of HIV infections in Ethiopia: Studies on the viral RNA stability and transmitted drug resistance in viral RNA and proviral DNA 2015 2

Immune reconstitution inflammatory syndrome

among HIV/AIDS patients during highly active antiretroviral therapy in Addis Ababa, Ethiopia 2007

Principal investigator 2015-/2016

Researcher and professor of medical virology 2015-2016

Team leader in the department of medical laboratory technology and School of health sciences 2005/2008 CLINICAL LABORATORY EXPERIENCES AND SKILLS

Extensive expertise in clinical laboratory including bacteriology, parasitology, hematology, clinical chemistry, immunohematology, immunology/serology, and urinalysis

Skills of clinical laboratory science such as monitoring the quality of testing, correlating test results with patient's condition, identifying abnormal cells, culturing & identifying bacteria, assuring safe transfusion of blood products, selecting & evaluating laboratory equipment & selecting, orienting & evaluating employees)

Performed Molecular and Cellular Biology Techniques including RT/PCR, cloning, sequencing, western blotting, cell culture & protein purification, dissecting & identification of medically important vectors

PEER-REVIEWED PUBLICATIONS

Kahsay Huruy, Andargachew Mulu, Uwe Gerd Liebert, Maier Melanie.HIV-1C proviral DNA for detection of drug resistance mutations. PLoS ONE (2018):13(10): e0205119.

Setegn Eshetie, Fentahun Tarekegn, Feleke Moges, Anteneh Amsalu, Wubet Birhan and Kahsay Huruy.Methicillin resistant Staphylococcus aureus in Ethiopia: a meta-analysis. BMC Infectious Diseases (2016) 16:689DOI 10.1186/s12879-016-2014-0.

FelekeMoges, SetegnEshetie,Mengistu Endris, Kahsay Huruy and DagnachewMuluye. 2016. Cockroaches as a Source of High Bacterial Pathogens with Multidrug Resistant Strains in Gondar Town, Ethiopia. Hindawi BioMed Research International.

Kahsay Huruy, Maier Melanie, Andargachew Mulu, Uwe Gerd Liebert. Limited increase in primary HIV-1C drug resistance mutations in treatment naïve patients in Ethiopia. J Med Virol. 2015 Jun; 87(6): 978-84.

FelekeMoges, Mengistu Endris, Andargachew Mulu, Belay Tessema, YeshambelBelyhun, YitayalShiferaw, Kahsay Huruy, Chandrashekhar Unakal, Afework Kassu. The growing challenges of antibacterial drug resistance in Ethiopia. Journal of Global Antimicrobial Resistance (2014),

Kahsay Huruy, Afework Kassu, Andargachew Mulu, NetsanetWorku, TeshomeFetene, Simon Gebretsadik, et al. Intestinal parasitosis and shigellosis among diarrheal patients in Gondar teaching hospital, northwest Ethiopia. BMC Res Notes. 2011, 31;4(1):472.

Andargachew Mulu,AfeworkKassu, Kahsay Huruy,

BirhanemeskelTegene,GashawYitayaw,Masayo Nakamori, et al.Vitamin A deficiency during pregnancy of HIV iinfected and non-infected women in tropical settings of Northwest Ethiopia. BMC Public Health. 2011, 15; 11:569.

Simon Gebretsadik, Tesfu Kassa, Haile Alemayehu, Kahsay Huruy,NigatuKebede.Isolation and characterization of Listeria monocytogenes and other Listeria species in foods of animal 3

origin in Addis Ababa, Ethiopia. J Infect Public Health. 2011; 4(1):22-9.

Kahsay Huruy, Afework Kassu, Andargachew Mulu, YematawWondie. Immune restoration disease and changes in CD4+ T-cell count in HIV- infected patients during highly active antiretroviral therapy at Zewditu memorial hospital, Addis Ababa, Ethiopia. AIDS ResTher. 2010, 21; 7:46.

GizachewBalew, NetsanetWorku, Tesfaye Tilaye, Kahsay Huruy, TeshomeFetene. Assessment of household burden of orphaning and coping strategies by guardians and families with orphans and vulnerable children in Hossana Town, SNNPR. Ethiop Med J. 2010; 48(3):219-28.

FantahunBiadglegne, Belay Tessema, MulugetaKibret, Kahsay Huruy, Belay Anagaw,AndargachewMulu. Physicochemical and bacteriological quality of bottled drinking water in three sites of Amhara Regional State, Ethiopia. Ethiop Med J. 2009;47(4):277-84.

TeshomeFetene, NestanetWorku, Kahsay Huruy, Nigatu Kebede. Cryptosporidium recovered from Muscadomestica, Muscasorbens and mango juice accessed bysynanthropic flies in Bahirdar, Ethiopia. Zoonoses Public Health. 2011;58(1):69-75.

NetsanetWorku, BirhanuErko, Workineh Torben, Mulgeta Belay, Afework Kassu, Kahsay Huruy. Malnutrition and intestinal parasitic infections in school children of Gondar, North West Ethiopia. Ethiop Med J.2009;47(1):9-16.

GashawAndargie, Afework Kassu, FelekeMoges, MogesTiruneh, Kahsay Huruy. Prevalence of bacteria and intestinal parasites among food-handlers in Gondar town, northwest Ethiopia. J Health Popul Nutr.2008; 26(4):451-5

Kahsay Huruy, Andargachew Mulu, Getahun Mengistu, Aster Showa-Amare, Addis Akalu,GashawAndargie, et al. Immune reconstitution inflammatory syndrome among HIV/AIDS patients during highly active antiretroviral therapy in Addis Ababa, Ethiopia.Jpn J Infect Dis. 2008;61(3):205-9.

Desta Ghebreselassie, Yalemtsehay Mekonnen, Girmai Gebru, Wondwossen Ergete, Kahsay Huruy. The effects of Moringastenopetala on blood parameters and histopathology of liver and kidney in mice. Ethiop J Health Dev. 2011;25(1):51-57.

FelekeMoges, YeshambelBelyhun, MogesTiruneh, Yenew Kebede, Andargachew Mulu,Afework Kassu, Kahsay Huruy. Comparison of formol-acetone concentration method withthat of the direct iodine preparation and formol-ether concentration methods for examination of stool parasites.Ethiop. J. Health Dev.2010;24(2):148-151.

TeshomeFetene, NetsanetWorku, Kahsay Huruy. Effect of NP fertilizers and cow dung on mosquitoes larval development in simulated field at Alage, central Ethiopia. J of Ethiop Journal of Health and Biomedical Sciences, 2009; 2(1):23-31.

Kahsay Huruy, Afework Kassu, Andargachew Mulu, Simon Gebretsadik, GashawAndargie,Takele Tadesse, et al. High level of antimicrobial resistance in Shigella species isolated from diarrhoeal patients, Gondar teaching hospital, Gondar Ethiopia. J of Pharmacologyonline;2,328-340,2008.

Kahsay Huruy, Afework Kassu, Andargachew Mulu, Workineh Torben, Afework Kassu.Review of Leishmaniasis in HIV infected individuals. J of Pharmacologyonline; 2, 59- 74,2008.

Andargachew Mulu, Afework Kassu, Kahsay Huruy,GobenaAmeni. Tuberculosis-Human immunodeficiency Virus Coinfection: bidirectional effect. J of pharmacology online; 2: 301- 318, 2008.

Kahsay Huruy, Afework Kassu, Andargachew Mulu. Seroprevalence and Risk Factors for Hepatitis B Virus Infection in the Healthy Blood Donors at Jimma University Hospital,Southwest Ethiopia. J of pharmacologyonline; 3:32-37,2008. 4

Afework Kassu, Masayuki Fujino, Masako Nishizawa, Getahun Mengistu, ErmiasDiro,BeleteAyele,DerejeKetema,FelekeMoges,AndargachewMulu,GizachewYismaw, Kahsay Huruy, Ebba Abate, Assefa Getachew, MogesTiruneh, YaredWondmikun,WataruSugiura, Fusao Ota. Levels of serum HIV-1 RNA viral load in tuberculosis patients with or without intestinal parasites during treatment of tuberculosis in Gondar,Ethiopia. Ethiop Journal of Health and Biomedical Sciences; 1(1), 5-13, 2007.

Amsalu Feleke, Yigzaw Kebede, AlemayehuMekonnen, ZelekeAlebachew, KassaWoreta, Kahsay Huruy,

BerhanemeskelWeldegerima,FekaduMazengia,YaredWondmikun,MensurOusman. Health Team Work and Team Training for the Ethiopian Health Center Team. Produced in collaboration with the Ethiopia Public Health Training Initiative, The Carter Center, the Ethiopia Ministry of Health, and the Ethiopia Ministry of Education. 2005.

Andargachew Mulu, Jemal Ali, Kahsay Huruy,AfeworkKassu. Laboratory diagnosis of HIV: Review. Focus on Gondar University, Chronicle 2004; 4: 261-268. MANUSCRIPTS (READY FOR SUBMISSION)

Kahsay Huruy, Maier Melanie, Stefan Schubert, Uwe Gerd Liebert. Stability of human immunodeficiency virus type 1 RNA in plasma and whole-blood samples treated with ethylene and propylene glycol and stored at variable temperature and time.

KahsayHuruy, Simon Gebretsadik, YeshambelBelyhun, TakeleTekilu, AregawiYalew. Intestinal parasitosis in relation to CD4+T cell counts and nutritional status in ART experienced and naïve HIV-1 infected patients in HIV clinic in Gondar teaching hospital, Ethiopia

MANUSCRIPTS (IN PREPARATION)

Kahsay Huruy, Maier Melanie, Uwe Gerd Liebert. HIV type 1 subtypes C tropism usage in therapy naïve Ethiopian patients, northwest Ethiopia.

Kahsay Huruy, Maier Melanie, Uwe Gerd Liebert. Comparison of HIV-1 viral load of dried blood spots stored for 5 years at 4 C with matched plasma viral loads.

Kahsay Huruy, Maier Melanie, SisayYifru, Uwe Gerd Liebert. Frequency of HIV-1,hepatitis B and C among visceral leishmaniasis infected patients and apparently healthy blood donors in northwest Ethiopia.

Kahsay Huruy, Indris Mehamed, Simon Gebretsadik, Suleman Isa, SisayYifru. Incidence and risk factors of immune reconstitution syndrome in HIV -1 infected patients during antiretroviral therapy at Gondar teaching hospital, northwest Ethiopia.

KahsayHuruy, Simon Gebretsadik, YeshambelBelyhun,TakeleTekilu, SisayYifru. Immune Reconstitution inflammatory syndrome among HIV-1 infected children during antiretroviral therapy at Gondar teaching hospital, northwest Ethiopia POSTERS/ORAL PRESENTATIONS AND CONFERENCE PROCEEDINGS

Kahsay Huruy. Limited increase in primary HIV-1C drug resistance mutations in treatment naïve patients in Ethiopia. Ethiopian Public Health Institute Consultative and dissemination workshop on HIV/AIDS and TB research agenda and research findings April 22-24, 2015, Mekelle, Ethiopia.

Kahsay Huruy. Immune restoration disease and changes in CD4+ T-cell count in HIV- infected patients during highly active antiretroviral therapy at Zewditu memorial hospital, Addis Ababa, Ethiopia (volume 6, number 2 Suppl 1). The 6th International Congress on Infectious and Parasitic Diseases Forum held from June 13-15 June 2013 in Kinshasa, Democratic Republic of Congo.

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Kahsay Huruy. Immune Reconstitution Inflammatory Syndrome among HIV/AIDS Patients during HAART in Addis Ababa. The 6th International Congress on Infectious and Parasitic Diseases Forum held from 13-15 June 2013 in Kinshasa, Democratic Republic of Congo. Ethiopia (volume 6, number 2 suppl 1).

Kahsay Huruy Special Lectures on Molecular studies on HIV-Leishmania co-infections. May 30, 2012, Faculty of Medicine, University of Leipzig.

Kahsay Huruy. The Immune reconstitution inflammatory syndrome among HIV/AIDS patients during highly active antiretroviral therapy in Addis Ababa, Ethiopia 6th European and Developing Countries Clinical Trials Partnership (EDCTP) Forumheld from 9 to 12 October 2011 in Addis Ababa, Ethiopia. (abstract number 012).

Kahsay Huruy. Tuberculosis Induced Immune Restoration Diseases among HIV/AIDS patients during Highly Active Antiretroviral Therapy (HAART) (abstract number 6).19th Ethiopian Public Health Association Annual Public Health Conference, 2008, Hawasa, Ethiopia.

Kahsay Huruy. Absence of pathogenic Escherichia coli O157:H7 among diarrheal patients in University of Gondar teaching hospital, Northwest Ethiopia (abstract number 10).18th Annual Student and Staff Research Conference, University of Gondar, 2008 Ethiopia.

Kahsay Huruy. High level of antimicrobial resistance in Shigellaspecies isolated from diarrhoeal patients in the University of Gondar teaching hospital, Gondar, Ethiopia (abstract number 2).18th Annual Student and Staff Research Conference, University of Gondar, 2008, Ethiopia.

Kahsay Huruy. Immune Reconstitution Inflammatory Syndrome among HIV/AIDS patients during Highly Active Antiretroviral Therapy (HAART) in Addis Ababa, Ethiopia (abstract number 85).18th Ethiopian Public Health Association Annual Public Health Conference, 2007, Addis Ababa, Ethiopia.

KahsayHuruy. Immune Reconstitution Inflammatory Syndrome among HIV/AIDS patients during Highly Active Antiretroviral Therapy (HAART) in Addis Ababa, Ethiopia (abstract number 9).17th Annual Student and Staff Research Conference, University of Gondar, Ethiopia, 2007.

FELLOWSHIPS AND AWARDS

Four grants from Gondar College of Medicine and Health Sciences, Research and publication office on infectious diseases projects 2005-2011

Scholarship for Ph.D. study in Medical Virology, Leipzig University, Germany

2010-2015

Scholarships for the M.Sc. degree in Tropical and Infectious Diseases, Addis Ababa University, Ethiopia 2005-2007

Certificate/license for international medical laboratory scientist from the American Society of Clinical Pathology (ASCP) 2018 PROFESSIONAL ACTIVITY, UNIVERSITY ACTIVITIES & SERVICE

PI and reviewer of mega projects related to HIV at the National Institute of public health in Ethiopia 2015-2016

Supervised undergraduate, M.Sc. and Ph.D. students while doing their projects and advising on issues related to selecting their research topics 2012-2016 6

I mentored more than 2000 health science students while they were doing their fieldwork related to their professional practicum in various health institutions and resettlement areas

I participated in heath science curriculum development at the national level… 2015-2016

An editorial consultant for the Ethiopian Journal of Laboratory Medicine (EJLM)

2015-2016

Delivered training on basics of Real-Time PCR and conventional RT/PCR for Early Infant HIV-1 diagnosis for Medical Laboratory Technologists from regional laboratories organized by Ethiopian Public Health Institute, Dec. 24-28, 2015, and January 1-5, 2016.

Chaired Mycobacterium Tuberculosis related presentations on the 20th annual conference on Laboratory Medicine: The Gate to better Health, May 9-10, 2015, Addis Ababa, Ethiopia.

An organizer for Dissemination Workshop on HIV and related diseases survey and surveillance, Dec10-12, 2015 prepared by Ethiopian Public Health Institute, Hawassa, Ethiopia.

Offered refreshment courses for medical laboratory science employees 2005/2016

Participating in national meetings related to HCV treatment program 2016

Involving in extra-curricular activities of the university 2004-2016

Participating as a volunteer in helping local NGOs associated with vulnerable children and women in Ethiopia 2008-2009/2015-2016 MEMBERSHIPS OF PROFESSIONAL ASSOCIATION:

American Society for clinical pathology (ASCP) 2018 – Present

Canadian Society for Medical Laboratory Science 2016 – Present

International Federation of Biomedical Laboratory Science …….2016 – Present

Lifelong member for Ethiopian Red Cross Society 2010 – Present

Ethiopian Medical Laboratory Association 2008 – Present

Ethiopian Public Health Association 2004 – Present RELEVANT COURSEWORK TAKEN

Virology and Rickettsiology, Immunology and Vaccinology, Emerging and Re-emerging Diseases, GI parasitic Diseases of Humans and Animals, Microbial Diseases of Humans and Animals, Zoonotic Diseases, Major Vector-borne Diseases, Advanced Diagnostic and Research Techniques, Lab. Management and Practice, Nutritional Disorders, Integrated, Vector Management, Molecular Biology, Microbiology, Bacteriology, Parasitology, Mycology, Haematology, Immunohematology, Clinical Chemistry, Histopathology Urinalysis REFERENCES

Dr. DawitWelday, Addis Ababa, Ethiopia; Email: ad0rme@r.postjobfree.com; Ph: 251 944- 731432

Dr. Ebba Abate, Ethiopian Public Health Institute, Addis Ababa, Ethiopia,Email: ad0rme@r.postjobfree.com; Ph: 251-***-******

Michael Hagos (M.Sc., P.Eng.),Senior Rating Engineer Manitoba Infrastructure, Email: ad0rme@r.postjobfree.com; Ph: 204- 794 -2247. Manitoba, Canada Address: 30217 13th Avenue South Federal Way,Washington 98003, USA Ph: +1-360-***-****

E-Mail: ad0rme@r.postjobfree.com/ad0rme@r.postjobfree.com

Kahsay Huruy Ghezehegn, Ph.D., MLS (ASCP).

HIGHLIGHTS OF EXPERTISE

Molecular methods: RT/PCR, cloning, sequencing, western blotting, cell culture & protein purification

Teaching & advising students of medical & health sciences of the college of medicine & health sciences, University of Gondar, Ethiopia

Research expertise on infectious diseases & related fields

Extensive expertise in clinical laboratory in different units such as specimen collection, antiretroviral therapy (virology lab), bacteriology, parasitology, hematology, clinical chemistry, immunohematology, immunology/serology, & urinalysis

Dissecting & identification of medically important vectors

Skills on SPSS, GraphPad Prism, Meta 11, Geneious, ViroSeq (HIV-1 Genotyping System), Molecular Evolutionary Genetics Analysis, etc

Completed NVCI training

Completed First Aid/CPR Training

Have Drivers License (Class 5)

In brief, I have the following experiences working as a clinical laboratory scientist

Identifying abnormal cells

Culturing & identifying bacteria

Assuring safe transfusion of blood products

Correlating test results with patient's condition

Selecting & evaluating lab equipment

Monitoring the quality of testing

Operating computerized instruments

Selecting, orienting & evaluating employees

CLINICAL LABORATORY SCIENTIST, RESEARCH AND TEACHING EXPERIENCE

Extensive expertise in clinical laboratory in different units such as specimen collection, antiretroviral therapy (virology lab), bacteriology, parasitology, hematology, clinical chemistry, immunohematology, immunology/serology, and urinalysis (briefly, the skills of clinical laboratory science I possess are: monitoring the quality of testing, correlating test results with patient's condition, identifying abnormal cells, culturing & identifying bacteria, assuring safe transfusion of blood products, selecting & evaluating laboratory equipment, operating computerized instruments, & selecting, orienting & evaluating employees).

As a researcher and professor of medical virology, I trained more than 1800 medical and health science students in various medical colleges and Universities. I taught subjects such as medical virology, advanced diagnostic microbiology, medical microbiology, bacteriology, immunology, mycology, medical parasitology, hematology, clinical chemistry, immunohematology, serology, and urinalysis. Each subject I instructed had significant laboratory practical sessions from basic laboratory practices up to advanced techniques such as molecular methods. More than 95% of my students passed their final examinations and were successful in their theoretical, practical, internal and external examinations.

I worked as principal investigator (PI) in HIV/TB department in Ethiopian Public Health Institute, which is a national research organization. I reviewed mega projects, conceived new projects, and revived a project that was discontinued due to lack of expertise. I also trained junior scientists and senior medical laboratory personnel on HIV-1.

I proposed different projects and published more than 20 scientific papers in peer-reviewed journals and also I published 1 book chapter. I served as a reviewer in various scientific journals such as International Journal of Microbiology, BioMed Research International journals, International Journal of Infectious Diseases (IJID), Journal of the International Association of Providers of AIDS Care (JIAPAC), International Journal of Microbiology and I also reviewed locally submitted proposals and manuscripts for consideration of funding and publication, respectively.

I led the department of medical laboratory technology and school of health sciences and supervised and mentored staffs of the clinical laboratory while they were analyzing clinical samples. I was a lead expert in the internal and external quality assurance unit of the teaching clinical laboratory hospital in the College of medicine and health sciences, University of Gondar, Ethiopia

I had a Student Assistant position for two years (2013-2014) in the Institute of Virology, Faculty of Medicine, Leipzig University, Germany.

In brief, my scientific contribution was important for developing countries like Ethiopia as well as other scientific communities of the world in formulating health policies.

PERTINENT ACHIEVEMENTS

Innovation of a new method to transport whole-blood specimens by treating with ethylene and propylene glycol from Ethiopia, Africa to Germany and I was able to extract RNA from whole-blood specimens stored for up to 18 months. In existing protocols collection of RNA from whole blood is challenging and the whole- blood needs to be separated to components immediately or up to 2 hours’ time for proviral DNA and HIV-1 RNA. This technique is significantly important to Ethiopia as well as other developing countries as transporting plasma/serum samples to referral laboratories is challenging due to frequent power outage and lack of facilities in the peripheral health laboratories to separate the whole-blood samples into components.

We found more mutations and higher frequency of polymorphisms in proviral DNA than HIV-1 RNA. This result may provide convenient tools that are suitable for Ethiopia as sequencing proviral DNA is more economical and user friendly than RNA samples.

Proportions of immune reconstitution inflammatory syndrome among HIV-1 infected patients during antiretroviral therapy were determined and this information has significant advantage for the policy makers in Ethiopia and was a seminal report in Ethiopia.

I was a PI and reviewer of mega projects related to HIV at the National institute of public health in Ethiopia.

I supervised undergraduate, M.Sc. and Ph.D. students while doing their projects and advising on issues related to selecting their research topics.

I mentored more than 2000 health science students while they were doing their fieldwork related to their professional practicum in various health institutions and resettlement areas.

I participated in heath science curriculum development at national level.

Certificate/license for international medical laboratory scientist from American Society of Clinical Pathology (ASCP)

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