Christopher A. Brooks

**** ******** **., ****** ****, FL 34607 281-***-**** ad0lb7@r.postjobfree.com

Objective

A position in business, research management or laboratory research using proven problem-solving, analytical, and interpersonal skills.

Education

MASTERS OF BUSINESS ADMINISTRATION 2016 WESTERN GOVERNORS UNIVERSITY

BACHELOR OF SCIENCE (BIOLOGY) 1989 UNIVERSITY OF HOUSTON

CERTIFICATE PROGRAM IN PUBLIC PROCUREMENT (CPPP) 2021, THE WORLD BANK

Skills & Abilities

·Experience with sourcing and vendor management

·Strong negotiation and analytical skills

·Excellent communication skills both written and verbal

·Ability to work in cross-functional teams to achieve positive results

·Developed and implemented individual and team strategies to reach profitable goals

·Proficient in Microsoft Office (Word, Powerpoint, Excel, and Access)

·Trained in the use of hazardous materials and safety techniques

·Demonstrated ability to learn and master new equipment and techniques

·Published world-class medical and agricultural researcher

·Electrophoresis, SDS-Page, PCR, Elisa, IHC, western blotting, bacterial & mammalian cell culture, gel filtration, DNA/RNA purification, laboratory equipment repair

Keywords

GLP, Healthcare, Supervisory experience, teaching, logistics, supply chain, contracts, SAP, manufacturing, leadership, budgeting, management, Microsoft office.

Experience

PROTEIN PURIFICATION SPECIALIST LIFE SCIENCES ADVANCED TECHNOLOGIES 2022 – PRESENT

·Performed exsanguination procedures on W36 chicken chicks.

·Design, implement, and optimize purification strategies to meet product design specifications.

·Troubleshoot technical issues, recommend and implement solutions.

·Performed assay to test and check concentrations of manufactured enzymes.

PROCUREMENT SPECIALIST II NOVA SOUTHEASTERN UNIVERSITY 2017 - 2022

·Responsibilities included sourcing and purchasing of supplies and services for all research, athletic, MRO (Maintenance, Repairs, & Operations), University grade schools, University medical, and dental clinics.

·Annual total monetary total of items purchased was well over $5,000,000.

·Purchasing supplies and equipment involved soliciting quotes and bids from vendors, inputting this data into NSU’s purchasing platform (Ariba) to generate a purchase order number, and an order to the vendor.

·In the performance of sourcing duties, I was able to save NSU more than $400,000 in revenue.

·The position also involved the resolution of invoice discrepancies in pricing and billing from vendors.

RESEARCH ASSISTANT II NOVA SOUTHEASTERN UNIVERSITY 2016 – 2017

·Negotiated with outside vendors on prices and quotes for laboratory supplies

·Performed all duties pertaining to ordering and inventory in the lab

·Cultured and propagated mammalian and bacterial cells

·Mammalian cells lines cultured were: NK-92, A-375, and 293

LAB MANAGER UNIVERSITY OF HOUSTON 2010 – 2015

·Managed the day to day operations of a multi-leveled, culturally diverse research laboratory

·Negotiated with outside vendors on prices and quotes for laboratory supplies and equipment resulting in cost savings of more than $5,000/month for the lab

·Developed and ordering system which eliminated costly duplicate orders

·Conducted bench research on estrogen receptors ER-alpha, and ER-beta, and liver receptors LXR-alpha, and LXR-beta.

·Procedures used were immunohistochemistry, phosphorescent imaging, and mammalian cell culture

·Trained new personnel hired to work in the laboratory

QUALITY CONTROL ASSOCIATE INTROGEN THERAPEUTICS 2007 – 2008

·Evaluated company drug candidate using various carcinoma cell lines (T-47, A549, SAOS, and 293)

·Collected data on the effectiveness of numerous manufactured drug lots

·Assays performed were Elisa’s, bioactivity, virus titers, and bio-burden

RESEARCH ASSOCIATE C-SIXTY INC. 2004 – 2006

·Conducted extensive research characterizing fullerene (nano-molecule) compounds

·Compounds were studied for solubility, absorption, and temperature stability

·Materials were investigated using mammalian cell culture, Elisa, western blotting, cytotoxicity assays, and cell staining

RESEARCH ASSOCIATE LEXICON GENETICS 2003 – 2004

·Performed quality control analysis on transformed baculovirus clones

·Developed a novel test to confirm whether transformation had taken place

·Transformation was confirmed using agarose and SDS-page gels

RESEARCH ASSOCIATE PRODIGENE INC. 1999 – 2003

·Determined protein concentration in transgenic corn kernels

·Analysis was achieved by HTS (high-through put screening) by hand and by liquid handler (robot)

·Screening was conducted by means of Elisa. Protein from transformants were isolated and verified using gel filtration and western blotting

·Specialty Elisa’s (both sandwich and competition) were developed from scratch to detect and quantitate various proteins produced by transgenic corn

RESEARCH ASSISTANT TEXAS A&M UNIVERSITY 1997 – 1999

·Inserted P. shermanii genes into E. coli bacteria

·Improved efficiency of Vitamin - B12 production in industrial strains

·Verified that insertions were successful by use of agarose and SDS-page gels

RESEARCH ASSISTANT TEXAS A&M UNIVERSITY 1995 – 1997

·Performed exotic experiments on living and sacrificed rats. Research conducted involved the study of circadian rhythms and other processes.

·Brain tissues studied were: cerebellum, hippocampus, and olfactory bulbs.

·On living anesthetized rats, certain areas of the brain were destroyed, and then cannulas were attached to the cranium using adhesive. Then rat stem cells were inserted into the damaged brain region, to determine if the damaged area could be repaired. Once the rats were sacrificed, staining studies were performed on brain sections.

RESEARCH ASSISTANT UNIVERSITY OF TEXAS MEDICAL BRANCH 1993 – 1995

·Research involved biochemical analysis of DNA repair enzymes.

·Using FPLC, and various columns (Mono-Q, Mono-S, Reverse phase, and others) purified DNA repair enzymes and other proteins.

·Characterized N-methlpurine-DNA glycosylase, a DNA repair enzyme.

RESEARCH ASSISTANT BAYLOR COLLEGE OF MEDICINE 1991 – 1992

·Cultured intestinal parasite polyps of T. solium and T. crassiceps.

·Using gel filtration and FPLC columns, proteins were isolated from homogenized tapeworm material and spent media.

·Developed Elisa’s to measure tapeworm protein and antigen levels in media.

RESEARCH ASSISTANT RICE UNIVERSITY 1989 – 1991

·Conducted screens of bacterial transformants.

·Using extracts, isolated and purified proteins using Ammonium sulfate, gel filtration, FPLC, and size exclusion membranes.

·Using DNA sequencing gels and sequencing software, DNA from transformants was identified, sequenced, and proteins verified.

PUBLICATIONS

Roy R, Brooks C, Mitra S. Purification and biochemical characterization of recombinant N – methylpurine – DNA glycosylase of the mouse. Biochemistry. 1994 Dec. 20; 33(50): 15131 – 40.

Streatfield S, Hood E, Howard J, Brooks C, et al. Corn as a production system for human and animal vaccines. Vaccine. 2003 Jan30; 21(7-8): 812 – 5.

Lamphear B, Streatfield S, Howard J, Brooks C, et al. Delivery of subunit vaccines in maize seed. Journal of Controlled Release. 2002 Dec 13; 85(1-3): 169-80.

Streatfield S, Lamphear B, Hood E, Howard J, Brooks C, et al. Development of an Edible Subunit Vaccine in corn against Enterotoxigenic strains of Escherichia Coli. In Vitro Cellular Development Biology of Plants. 38: 11 – 17.

Streatfield S, Magallanes-Lundback ME, Brooks CA, et al. Analysis of maize polyubiquitin- 1 promoter heat shock elements and generation of promoter variants with modified expression characteristics. Trangenic Res. 2004 Aug: 13 (4): 299-312.

Lamphear BJ, Barker DK, Brooks CA, et al. Expression of the sweet protein brazzein in maize for production of a new commercial sweetener. Plant Biotechnol J. 2005 Jan: 3(1): 103 - 14.

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