Sheryl A. Garrovillo

***** ******* ***** **. *

San Diego, CA 92126

Tel # 619-***-****

ad0hxj@r.postjobfree.com

Summary:

Excellent ability to prepare histologic slides containing human tissue and animal tissues

Goal-oriented individual with strong leadership capabilities

Organized, highly motivated, multitasking and very trainable technician

Proven ability to work in high-volume laboratory

Ability to communicate effectively in English, both verbally and in writing

Thirteen years experienced as histology technician

Eligible for HT/HTL (ASCP) registration.

Educational Qualification:

Completed Bachelor’s degree in Medical Technology in year 2000 from Silliman University, Dumaguete City, Philippines.

Professional Experienced:

1.Scientist

Reveal Biosciences

September 2021- Present.

2.Senior Associate Scientist

May 2019-Aug. 2021

Janssen Pharmaceutical

Responsible to provide guidance to Research associate.

Responsible for operating, order reagents of Ventana machine, Dako autostainer, Leica Bond Rx, Sakura H&E autostainer.

• Responsible for hands-on implementation of the histology laboratory providing high quality histology services

• Prepare tissues for histological evaluation including use of immunohistochemistry staining techniques to support preclinical development and collaborations

• Representative of histology group for Protocol Reviews

• Team lab. Captain for Research Operational Team

• Support vivarium necropsy on large studies and train other associates on optimal tissue embedding techniques for optimal histology read out

• Develop, optimize antibody, cross-reactivity studies and validate different immunohistochemistry techniques and staining methods for preclinical and clinical studies

• Prepare tissues for IHC: grossing, fixing, processing, embedding, trimming and sectioning

• Perform histochemistry, immunohistochemistry, immunofluorescence, FISH/ISH based assays

• Perform paraffin and cryostat sectioning

• Validate and troubleshoot technical problems in assay development

3.SENIOR HISTOLOGY TECHNICIAN 11

May 5, 2014—2019

Halozyme Therapeutics, Inc.

Responsible for hands-on implementation of the histology laboratory providing high quality histology services

Prepare tissues for histological evaluation including use of immunohistochemistry staining techniques to support preclinical development and collaborations

Representative of histology group for Protocol Reviews

Team lab. Captain for Research Operational Team

Support vivarium necropsy on large studies and train other associates on optimal tissue embedding techniques for optimal histology read out

Develop, optimize antibody, cross-reactivity studies and validate different immunohistochemistry techniques and staining methods for preclinical and clinical studies

Prepare tissues for IHC: grossing, fixing, processing, embedding, trimming and sectioning

Perform histochemistry, immunohistochemistry, immunofluorescence, FISH/ISH based assays

Perform paraffin and cryostat sectioning

Validate and troubleshoot technical problems in assay development

Maintaining inventory of paraffin blocks and slides

Archiving blocks and stain/unstained slides

Stocking, inventory and order lab supplies

Manage histo lab equipment and microscopes for routine PM services

Helps in developing multiplex staining in Leica Bond and Ventana Machine

4.Histologic Technician

Dec. 10, 2012-December 2017

Rady Children’s Hospital

Processes tissues, embeds and sections Surgical Pathology cases/GI Biopsy

Performs routine, special and immunohistochemistry stains

Mounts, labels and distributes microscopy slides to the Pathologists

Completes and maintains PM/QC records. Performs general preventative maintenance on laboratory equipment

Accession specimens

Assist the Pathologist to embed and cut frozen tissues and stain

Prepare slides for cytology, Assist in autopsy

Doing protocol reviews and revisions

Assist in preparing the lab CAP inspections

Doing IHC validations for new antibodies

5.Research Associate iii (contract worker)

May 23, 2011--May 24, 2013

Johnson and Johnson PRD La Jolla, CA

Printing/ making glass slides and cassettes

Embedding using Sakura tissue-tek

Sectioning using Leica semi-automated microtome

H&E staining using Sakura tissue-tek Prisma.

Doing IHC using Dako Autostainer

Coverslipping using Sakura tissue-tek Glas

QC for IHC staining

Purchasing for all necessary lab reagents/ materials

Tissue block/ slides filing

Assisting in necropsy, cutting frozen tissue

Supports Toxicology studies by doing grossing, sectioning, staining and IHC

Helps in developing double staining

6. Histology Technician

March 31, 2005 till May 06,2011

San Diego Pathologists Medical Group, Inc.

Lead histology technician in immunohistochemistry department

Embedding, cutting, staining and preparing tissue/biopsies samples

Preparing requisition slip from pathologists

Prepares dilutions for antibodies, and reagents for staining

Doing a high volume of immunohistochemistry staining

Checking and assisting co-workers, and maintaining the work flow

Assuring that quality control criteria are met

Recording the internal and external problem

Publications and Posters

Ma JY, Snook S, Garrovillo S, Johnson C, La D.

“An Immunohistochemical Investigation of Renal Phospholipidosis and Toxicity in Rats.” Int. J. Toxicol.2017:36(5)386-394

CM Zhao, B Thompson, K Chen, M Marella, S Zimmerman, T Kimbler, B Blouw, S Garrovillo, L Huang, A Radi, ZD Huang, HM Shepard, S Rosengren, C THanos, D Maneval. “ The Growth of A Xenograft Breast Cancer Tumor Model with Engineered Hyaluronan-accumulating Stroma is dependent on Hyaluronan and Independent of CD44. AACR Annual Meeting 2018

D Taverna, S Garrovillo, B Thompson, D Kang. “Assessment of the Accumulation of Hyaluronan in the Tumor Microenvironment (TME) of Solid Tumors. EACR25 2018

B Thompson, JS Lee, R Clift, D Taverna, S Garrovillo, BBlouw, D Kang, C Thompson, D Maneval. “ Remodeling of the Tumor Microenvironment by Pegvorhylurondiase Alfa (PEGPH20): A novel, First-in-class biologic that enzymatically degrades tumor hyaluronan (HA) to improve anti-tumor efficacy.

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