Mana Oloomi

604-***-****

ad09y4@r.postjobfree.com

**** *********, **** ***** ***.

West Vancouver, BC

V7S2W1

EMPLOYMENT

From 2000-2023 Pasteur Institute of Iran

• 2000-2011: Assistant Professor

• 2011-2016: Associate Professor

• 2016: Full Professor

EDUCATION

1984-1989: Tehran University, Faculty of Science

• B.Sc. in Cell and Molecular Biology

1989-1992: Tehran University, Faculty of Medicine

• M.S. in Immunology

1993-1998: Pasteur Institute of Iran, Tehran

• Ph.D. in Medical Biotechnology

2000-2001: Pasteur Institute of Paris, Paris

• Postdoctoral Fellowship in Signal Transduction

Teaching experience

• Genetic Engineering

• Biotechnology

• Teaching M.Sc. and Ph.D. candidates

Job Description

As a lead researcher and principal investigator, my duties include structuring Ph.D. research projects, mentoring and supervising Ph.D. and M.Sc. candidates in writing and approving their pre-proposals. Successful proposals receive grants for research under my supervision, with the goal of publishing the research findings. Area of research

My research expertise includes Molecular Biology, Medical Biotechnology, and Genetic Engineering, specifically focusing on molecular cloning and expression of proteins and peptides from human and bacterial sources (diarrheal disease). I have applied these proteins as fusion chimeric proteins in vaccine development and cancer detection and treatment.

Publications:

1. Akhavan Attar F, Oloomi M*, Irani S, Azizi M, Geranpayeh L. "LncRNAs AK058003 and MVIH Overexpression in the Blood Samples of Iranian Breast Cancer Patients." J. 2021 Mar 1; 25(2):93-8. 2. Oloomi M*, Javadi M, Asadi Karam MR, Khezerloo JK, Haghri Z, Bouzari S.

"Protective multi-epitope candidate vaccine for urinary tract infection." Biotechnol Rep (Amst). 2020 Nov 26;28:e00564.

3. Pourramezan Z, Oloomi M, Kasra-Kermanshahi R. "Antioxidant and Anticancer Activities of Lactobacillus Hilgardii Strain AG12a." Int J Prev Med. 2020 Sep 3; 11:132.

4. Moazzezy N, Rismani E, Rezaei M, Karam MRA, Rafati S, Bouzari S, Oloomi M*. "Computational evaluation of modified peptides from human neutrophil peptide 1 (HNP-1)." J Biomol Struct Dyn. 2020 Sep 26:1-9. 5. Moazzezy N, Asadi Karam MR, Rafati S, Bouzari S, Oloomi M*. "A Synthetic Peptide 2Abz(23)S(29) Reduces Bacterial Titer and Induces Pro- Inflammatory Cytokines in a Murine Model of Urinary Tract Infection." Drug Des Devel Ther. 2020 Jul 17; 14

6. Javadi M, Oloomi M, Bouzari S. "In Silico Design of a Poly-epitope Vaccine for Urinary Tract Infection Based on Conserved Antigens by Modern Vaccinology." International Journal of Peptide Research and Therapeutics, 2020,1-13.

7. Moazzezy N, Asadi Karam MR, Rafati S, Bouzari S, Oloomi M 8.

Publications:

scholar.google.com citations

Mana Oloomi - Google Scholar

https://scholar.google.com/citations?user=uiv-elgAAAAJ https://pubmed.ncbi.nlm.nih.gov/?term=oloomi+Pasteur&sort=date 1. Karimi N, Oloomi M*, Orafa Z. Circulating Tumor Cells Detection in Patients with Early Breast Cancer Using MACS Immunomagnetic Flow Cytometry. Avicenna J Med Biotechnol. 2020 Jul-Sep;12(3):148-156. 2. Oloomi M*, Moazzezy N, Bouzari S. Comparing blood versus tissue-based biomarkers expression in breast cancer patients. Heliyon. 2020 Apr 3;6(4):e03728.

3. Moazzezy N, Bouzari S, Oloomi M*. Comparative Study of Blood, Tissue and Serum Levels of Carcinoembryonic Antigen (CEA) Detection in Breast Cancer. Asian Pac J Cancer Prev. 2019 Oct 1;20(10):2979-2985. 4. Tavakoli M, Bouzari S, Jafari A, Oloomi M, Asadi Karam MR, Najar-Peerayeh S, Siadat SD. Effect of nontypeable Haemophilus influenzae protein E (PE) as a microbial adjuvant on the amount of antibody against PRP of Haemophilus influenzae type b (Hib) in BALB/c mice. Microb Pathog. 2019 Apr;129: 78-81. 5. Oloomi M*, Imani M, Behzadi R, Asori M, Bouzari S, Mokhlesi B. Anti-tumor activity of Escherichia coli Shiga toxin A subunit delivered by SF9 insect cells. J Pharmacol Sci. 2018 Sep;138(1):71-75.

6. Babakhani S, Oloomi M. Transposons: the agents of antibiotic resistance in bacteria. J Basic Microbiol. 2018 Nov; 58(11):905-917. 7. Oloomi M*, Moazzezy N, Bouzari S. Modulation of Molecular Biomarker Expression in Response to Chemotherapy in Invasive Ductal Carcinoma. Biomed Res Int. 2018 Feb 12; 2018:7154708.

8. Behrouzi A, Bouzari S, Oloomi M, Fateh A, Vaziri F, Afrough P, Amanzadeh A, Siadat SD. In silico design, cloning, expression and immunologic evaluation of ED fusion protein of NT H. influenzae. Microb Pathog. 2017 Dec; 113:472-479. 9. Amraee M, Oloomi M*, Yavari A, Bouzari S. DNA aptamer identification and characterization for E. coli O157 detection using cell based SELEX method. Anal Biochem. 2017 Nov 1; 536:36-44.

10. Valiyari S, Salami M, Mahdian R, Shokrgozar MA, Oloomi M, Mohammadi Farsani A, Bouzari S. sIL-24 peptide, a human interleukin-24 isoform, induces mitochondrial-mediated apoptosis in human cancer cells. Cancer Chemother Pharmacol. 2017 Sep;80(3):451-459.

11. Javadi M, Oloomi M*, Bouzari S. In Silico Signature Prediction Modeling in Cytolethal Distending Toxin-Producing Escherichia coli Strains. Genomics Inform. 2017 Jun; 15(2):69-80.

12. Pourramezan Z, Kasra Kermanshahi R, Oloomi M, Aliahmadi A, Rezadoost H. In vitro study of antioxidant and antibacterial activities of Lactobacillus probiotic spp. Folia Microbiol (Praha). 2018 Jan;63(1):31-42. 13. Golshani M, Oloomi M*, Bouzari S. In silico analysis of Shiga toxins (Stxs) to identify new potential vaccine targets for Shiga toxin-producing Escherichia coli. In Silico Pharmacol. 2016 Dec;5(1):2.

14. Jeiranikhameneh M, Razavi MR, Irani S, Siadat SD, Oloomi M. Designing novel construction for cell surface display of protein E on Escherichia coli using non- classical pathway based on Lpp-OmpA. AMB Express. 2017 Dec; 7(1):53. 15. Behrouzi A, Bouzari S, Siadat SD, Oloomi M, Davari M, Mazaheri H. Evaluation of the immunogenic property of NT H. influenzae protein D with Neisseria meningitidis OMV in BALB/c. J Infect Dev Ctries. 2016 Dec 30;10(12):1345- 1351.

16. Keyvani S, Karimi N, Orafa Z, Bouzari S, Oloomi M*. Assessment of Cytokeratin-19 Gene Expression in Peripheral Blood of Breast Cancer Patients and Breast Cancer Cell Lines. Biomark Cancer. 2016 Apr 28;8:57-63. 17. Abedi Jafari F, Oloomi M*, Bouzari S. Comparative Effect of Recombinant Shiga Toxin in Induction of Pro- and Anti-Apoptotic Markers and Inflammatory Cytokines in Epithelial and Monocytic Cells. Jundishapur J Microbiol. 2016 Feb 28; 9(2):e24758.

18. Moazzezy N, Ebrahimi F, Sisakht MM, Yahyazadeh H, Bouzari S, Oloomi M.* Relationship between erb-B2 mRNA Expression in Blood and Tissue of Invasive Ductal Carcinoma Breast Cancer Patients and Clinicopathological Characteristics of the Tumors. M. Asian Pac J Cancer Prev. 2016; 17(1):249-54. 19. Oloomi M*, Javadi M, Bouzari S. Presence of Pathogenicity Island related and plasmid encoded virulence genes in cytolethal distending toxin producing Escherichia coli isolates from diarrheal cases. Int J Appl Basic Med Res. 2015 Sep-Dec; 5(3):181-6.

20. Davoodabadi A, Abbaszadeh M, Oloomi M, Bouzari S. Phenotypic and Genotypic Characterization of Enteroaggregative Escherichia coli Strains Isolated From Diarrheic Children in Iran. Jundishapur J Microbiol. 2015 Sep 8; 8(9):e22295.

21. Golshani M, Rafati S, Dashti A, Gholami E, Siadat SD, Oloomi M, Jafari A, Bouzari S. Vaccination with recombinant L7/L12-truncated Omp31 protein induces protection against Brucella infection in BALB/c mice. Mol Immunol. 2015 Jun;65(2):287-92

22. Habibi M, Asadi Karam MR, Shokrgozar MA, Oloomi M, Jafari A, Bouzari S Intranasal immunization with fusion protein MrpH·FimH and MPL adjuvant confers protection against urinary tract infections caused by uropathogenic Escherichia coli and Proteus mirabilis. Mol Immunol. 2015 Apr;64(2):285-94. 23. Mana Oloomi, Saeid Bouzari, Maryam Imani, Narges Akhtarian. Construction of a Baculovirus vector containing A subunit of Shiga toxin for protein delivery. Iranian Journal of Microbiology 2013. 5(4):350-355. 24. Mohammad Mohammadzadeh, Mana Oloomi, saeid Bouzari. Genetic evaluation of Locus of enterocyte effacement pathogenicity island (LEE) in Enteropathogenic Escherichia coli isolates (EPEC). Iranian Journal of Microbiology 2013. 5(4):345-349.

25. Moazzezy N, Oloomi M, Bouzari S. Effect of shiga toxin and its subunits on cytokine induction in different cell lines. Int J Mol Cell Med. 2014 Spring; 3(2):108-17.

26. Moazzezy N, Farahany TZ, Oloomi M, Bouzari S.Relationship between preoperative serum CA 15-3 and CEA levels and clinicopathological parameters in breast cancer. Asian Pac J Cancer Prev. 2014;15(4):1685-8. 27. Jafari A, Shafaei E, Oloomi M, Aghasadeghi MR, Bouzari S. Genotypic and Phenotypic Comparison of Enteroaggregative Escherichia coli Isolates from HIV- Positive and Non-HIV Diarrheal Samples. Curr HIV Res. 2013 Dec;11(8):635-41. 28. Oloomi M, Yardehnavi N, Bouzari S, Moazzezy N. Non-coding CK19 RNA in peripheral blood and tissue of breast cancer patients. Acta Med Iran. 2013 Mar 16;51(2):75-86.

29. Karam MR, Oloomi M, Mahdavi M, Habibi M, Bouzari S. Vaccination with recombinant FimH fused with flagellin enhances cellular and humoral immunity against urinary tract infection in mice. Vaccine. 2013 Feb 6; 31(8):1210-6. 30. Karam MR, Oloomi M, Mahdavi M, Habibi M, Bouzari S. Assessment of immune responses of the flagellin (FliC) fused to FimH adhesin of Uropathogenic Escherichia coli. Mol Immunol. 2012 Nov 30; 54(1):32-39. 31. Jahanian-Najafabadi A, Bouzari S, Oloomi M, Roudkenar MH, Shokrgozar MA. Assessment of selective toxicity of insect cell expressed recombinant A1-GMCSF protein toward GMCSF receptor bearing tumor cells. Res Pharm Sci. 2012 Jul; 7(3):133-40.

32. Asadi KM, Oloomi M, Habibi M, Bouzari S. Cloning of fimH and fliC and expression of the fusion protein FimH/FliC from Uropathogenic Escherichia coli

(UPEC) isolated in Iran. Iran J Microbiol. 2012 Jun;4(2):55-62 33. Oloomi M*, Bouzari S, Mohagheghi MA, Khodayaran-Tehrani H. Molecular Markers in Peripheral Blood of Iranian Women with Breast Cancer. Cancer Microenviron. 2013 Apr;6(1):109-16.

34. Noroozian Z, Oloomi M*, Bouzari S*. Analyzing of expression of novel polypeptide complexes consisting of Shiga toxin B subunit and Adherence Fimbriae of Escherichia coli based on in silico modeling. J Mol Model. 2012 Apr 24.

35. Jahanian-Najafabadi A, Bouzari S*, Oloomi M*, Habibi Roudkenar M, Mayr LM. Attempts to Express the A1-GMCSF Immunotoxin in the Baculovirus Expression Vector System. Biosci Biotechnol Biochem. 2012 Apr 23;76(4):749- 54

36. Bouzari S, Aslani MM, Oloomi M, Jafari A, Dashti A. Comparison of multiplex PCR with serogrouping and PCR-RFLP of fliC gene for the detection of enteropathogenic Escherichia coli (EPEC). Braz J Infect Dis. 2011 Aug; 15(4):365-9.

37. Oloomi M, Bouzari S. Characterization of Rabbit Polyclonal Sera against Recombinant Shiga Toxin and its Subunits for Detection of Stx-Producing E. coli. Iran J Allergy Asthma Immunol. 2011 Mar; 10(1):41-6. 38. Oloomi M, Bouzari S, Shariati E. In vivo characterization of fusion protein comprising of A1 subunit of Shiga toxin and human GM-CSF: Assessment of its immunogenicity and toxicity. Iran Biomed J. 2010 Oct; 14(4):136-41. 39. Asadi Karam M, Bouzari S, Oloomi M, Aslani M, Jafari A. Phenotypic and Genotypic Characterization of Enteropathogenic Escherichia coli (EPEC) strains in Tehran, Iran. Iran J Microbiol. 2010 Mar;2(1):3-7. 40. Oloomi M, Bouzari S. Assessment of immune response of the B subunit of Shiga toxin fused to AAF adhesin of enteroaggregative Escherichia coli. Microb Pathog. 2011 Mar-Apr; 50(3-4):155-8.

41. Oloomi M, Bouzari S, Emami S. A recombinant hybrid peptide composed of AAF adhesin of enteroaggregative Escherichia coli and Shiga toxin B subunit elicits protective immune response in mice. Eur J Clin Microbiol Infect Dis. 2009 Nov; 28(11):1311-6.

42. Bouzari S, Oloomi M*, Azadmanesh K. Study on induction of apoptosis on HeLa and Vero cells by recombinant shiga toxin and its subunits. Cytotechnology. 2009 Aug 9.

43. Bouzari S, Dashti A, Jafari A, Oloomi M. Immune response against adhesins of enteroaggregative Escherichia coli immunized by three different vaccination strategies (DNA/DNA, Protein/Protein, and DNA/Protein) in mice. Comp Immunol Microbiol Infect Dis. Infect Dis. 2010 May; 33(3):215-25. 44. Oloomi M*, Bouzari S, Ajdary S. Immune responses of mice immunized with active recombinant shiga toxin and its derivatives. Iran J Allergy Asthma Immunol. 2008 Jun; 7(2):53-60. 17.

45. Roudkenar MH, Bouzari S, Kuwahara Y, Roushandeh AM, Baba T, Oloomi M, Fukumoto M. Induction of apoptosis on K562 cell line and double strand breaks on colon cancer cell line expressing high affinity receptor for granulocyte macrophage-colony stimulating factor (GM-CSF). Iran Biomed J. 2008 Jan; 12(1):1-6.

46. Oloomi M, Bouzari S.Molecular profile and genetic diversity of cytolethal distending toxin (CDT)-producing Escherichia coli isolates from diarrheal patients. APMIS. 2008 Feb; 116(2):125-32.

47. Mana Oloomi*, Saeid Bouzari, Mania Arshadi. Leader sequence of Shiga toxin

(Stx) 1 is essential for periplasmic expression of recombinant active protein produced in E. coli. Protein Pept Lett, 13(5): 509-512, 2006. 48. M. Oloomi and S. Bouzari. Comparison of PCR systems for detection of different cdt genes in Escherichia coli strains. Lett Appl Microbiol 42(5): 445-451, 2006. 49. M. Habibi Roudkenar, S. Bouzari*, Y. Kuwahara, A. Mohammadi Roushandeh, M. Oloomi, M. Fukumoto. Recombinant Hybrid Protein, Shiga-Toxin-GM-CSF, Effectively Induces Apoptosis of Colon Cancer Cells. World J Gasteroenterol 12(15): 2341-2344, 2006.

50. Mehryar Habibi Roudkenar, Anis Jafari, Mana Oloomi, Saeid Bouzari. Selective cytotoxicity of recombinant STXA1-GMCSF Protein in hematopoetic cancer cells Cell Biol Toxicol 22(3): 213- 219, (2006)

51. Mehryar Habibi Roudkenar, Saeid Bouzari, Mana Oloomi, Anis Jafari, Nader Shahrokhi and Ali Shokrgozar. Expression of a chimeric protein containing the catalytic domain of shiga-like toxinand human Granulocyte Macrophage colony- stimulating factor (hGM-CSF) in Escherichia coli and its recognition by reciprocal antibodies. Iran. Biomed. J. 9 (4):143-148, 2005. 52. S. bouzari, M. Oloomi, Eric Oswald, Detection of CytolethalDistending Toxin Locus cdtB among Diarrhoeagenic Escherichiacoli isolates from Humans in Iran. Res. Microbiol. 156:137-144,2005.

53. Bouzari S, Oloomi M, Oswald E.Detection of the cytolethaldistending toxin locus cdtB among diarrheagenic Escherichia coliisolates from humans in Iran.Res Microbiol. 2005 Mar;156(2):137- 44.

54. M. Oloomi*, S. Bouzari, Improvement of hGM-CSF expression, using fusion system by directing the protein to periplasmic space. Medical Journal of IRI

(MJIRI), 18, (2) 141-146, 2004.

55. S. Bouzari, A. Jafari, A. Azizi, M. Oloomi, and J.P. Nataro. Characterization of Enteroaggregative Escherichia coli isolates from Iranian children. Am. J. Trop. Med. Hyg., 65 (1), 13-14. 2001.

56. Saeid Bouzari, Mana Oloomi, Ali Hatef Salmanian and Anis Jafari, Construction and Expression of a Fused Gene for B Subunit of the Heat-Labile and a Truncated form of the Heat-Stable Entrotoxins in Escherichia coli, Iranian Biomedical J.,3

(3):83-86 (October) 1999.

57. Oloomi M.*, Bouzari S., Rechinsky V., Purification and characterization of the cloned human GM-CSF gene expressed in Escherichia coli, MJIRI, 12, (4): 353- 357, 1999.

58. Oloomi M.*, Bouzari S., Rechinsky V., PCR-mediated expression of the human GM-CSF gene in Escherichia coli, Iranian Biomedical J., 2 (1): 21-25 (January 1998).

59. Mana Oloomi*, Saeid Bouzari, Afsaneh Rassaeian, Mohammad- Ali Mohagheghi MA, Evaluation of CK-19 BioMarker in peripheral blood of breast cancer patients. Iranian Journal of Surgery, Vol. 19, No. 1, 2011 Published Contributions to International Conferences: 1- Oloomi M., Rafiei Sh., Is Psoriasis an auto immune disease, The Third Iranian Congress of Immunology and allergy. Shiraz, Iran, 7-10 May 1995. 2-Oloomi M., Bouzari S., Rechinsky V., Cloning, Expression and purification of the human GM-CSF as an important haemopoetic Factor, The Fourth International congress of Immunology & Allergy, Iran, Isfahan 13-15 May 1998.

3- Oloomi M., Bouzari S., Comparison the level of hGM-CSF expression in two different systems in Escherichia coli, Proceeding of the First National Congress of Biotechnology, Tehran 22-24 Feb 2000.

4- M.Algarte, T.Yeh, J.Ragimbeau, M. Oloomi et S.Pellegrini.Identification de nouveaux partenaires de Tyk2 par criblage double hybride dans la levure.Institut Pasteur 22-23 Nov. 2000.

5- Michele Algarte, Tammie Yeh, Mana Oloomi & sandra Pellegrini. Identification of new Tyk2 interacting proteins.Saint-MaloSociete Francaise de Biochimie et Biologie Moleculaire, 2-4 Oct. 2000.

6- M.Oloomi, S. Bouzari, Increasing the efficiency of protein production by periplasmic expression in E. coli Biochemical Society Transactions (2002) Volume 30, Part 6, p. A130. Biochemical Society 2002 Wales, England. 7- Oloomi, M. Bouzari, S. Arshadi, M. cloning and Expression of recombinant Shiga toxin Stx1-A and Stx1-B subunits in E. coli. The 7th Iranian congress of Microbiology 1- 3 Feb. 2005.

8- Bouzari, S., Jafari, A. Asadi-Karam, M.R. Asalni, M.M. Oloomi, M. Shahrokhi, N. Detection and Molecular Typing of Attaching and Effacing Gene in Diarrheagenic E. coli the 7

th

Iranian congress of Microbiology 1- 3 Feb. 2005.

9- Bouzari, S., Jafari, A. Asadi-Karam, M.R. Asalni, M.M. Oloomi, M. Shahrokhi, N. Dashti, A. Molecular Characterization of Diarrheagenic Escherichia coli International Journal of Infectious Diseases Lisbon Portugal, June 15-18, 2006. 10- Mana Oloomi, Saeid Bouzari, Soheila Ajdari, Immunological studies of recombinant shiga toxin and its subunits. 1

st

joint Meeting of European National Societies of

Immunology & 16

th

European Congress of Immunology, 6-9 Sep. 2006. Paris, France 11- Oloomi M, Bouzari S, Rassaeian A, Salamati M, Parivar K. Molecular Evaluation of Peripheral Blood cytokeratin-19 (CK-19) in normal and malignant Breast disease. 2nd International student conference of Biotechnology 15-17 Nov. 2008, Tehran University 12. Oloomi M, Bouzari S, Mohagheghi MA, Study of CK19, Her2/neu and Mammoglobin expression in benign breast cancer. Iranian Journal of Breast Diseases Winter 1387 85-S-09

13. Oloomi M, Bouzari S, Rassaeian A, Mohagheghi MA, 14. M. Oloomi, S. Bouzari, N. Moazzezy. Stimulation of apoptosis factors and inflammatory cytokine release by recombinant Shiga toxin 4 th

Congress of European

Microbiologists, FEMS 2011 Geneva, Switzerland June 26-30, 2011 15. S. Bouzari, B. Kashef, MM Aslani, M. Oloomi. Presence of virulence associated genes and clonal nature of enteroaggregative E. coli (EAEC) isolates from Diarrheal diseases.

Grants:

- FEBS Practical / Lecture Course Human and Microbial Genomics: DNA Arrays applied to Human Pathogens and Diseases, March 30th –April 7th, 2006. With the support of Pasteur Institute of Paris, Hosted by Hellenic Pasteur Institute, Athens, Greece

- Research grant of Eastern Mediterranean Health Genomics & Biotechnology Network-EMHGBN (EMGEN), Grant No. 20094 Molecular Markers detection in Breast cancer. 2009-2011

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