State University Research

Afzaal Nadeem Mob:+1-662-***-****

*** * ******* ** *****:*************@*****.***

Starkville, MS

PROFESSIONAL SUMMARY

Mid-Level Researcher with 6+ years of lab experience centering on the following: Toxicology/Behavioral neuroscience/data analysis/Molecular Biology/Environmental Science

EDUCATION

Doctor of Philosophy in Environmental toxicology (pursuing) Mississippi State University, USA.

Master of Science in Environmental science

Southern Illinois University Edwardsville, USA.

Bachelor of Technology in Biotechnology

Jawaharlal Nehru technological university, India.

TECHNICAL SKILLS

Software/Databases: MS Office, SPSS, SAS

Programming languages: C (Basic knowledge), Perl (Intermediate) PROFESSIONAL EXPERIENCE

MISSISSPPI STATE UNIVERSITY Jan 2014-Present

Research Assistant, Center for Environmental and Health Sciences, Mississippi State University HANDS ON EXPERIENCE WORKING WITH RODENTS

Responsibilities:

• Breeding rats and assigning pups to different dosing groups.

• Extensive dosing rats with Organophosphates, Pyrethroids, and Tetrahydrocannabinoids (THC).

• Behavioral experiments (emergence test, EPM, Social interaction, Social preference, T-maze).

• Dissecting rats and isolating brain, liver, spleen, and serum samples.

• Hands on experience on brain slicing on cryostat and Collecting specific brain region by using LCM.

• RNA extraction from specific brain region (amygdala), running RNA on agarose gel and send it for sequencing.

• Analyzing sequencing data (Perl programming, TUXEDO protocol, Gene Ontology, Ingenuity pathway analysis).

• Enzyme assays (activity assay, cholinesterase, carboxylesterase on brain, liver and serum samples).

Afzaal Nadeem Mob:+1-662-***-****

319 N Jackson St Email:*************@*****.***

Starkville, MS

DISSERTATION TOPIC:

Investigating the mechanism of permanent behavioral alterations in rats exposed to low level of organophosphate insecticides during neonatal ages. GRADUATE COURSES:

Environmental Toxicology, Organ System Tox I and II, Mechanism of Toxic Action, Research Ethics, Biochemistry I & II, Biocomputing (Perl), Computational Biology, Manuscript Writing, Neuroscience, Biostatistics (SAS), seminar courses. TRAININGS:

Introduction to lab safety, radiation safety, hazardous waste management, animal user certification, human subject training, occupational health and safety. SOUTHERN ILLINOIS UNIVERSITY EDWARDSVILLE Jan 2011-May 2013 Research Assistant

• Maintained lab safety by entering chemical toxicity information MSDS sheet, keeping record of all chemicals, receiving orders.

• Chronic dosing zebrafish with thyroid modulating chemicals, stagnant voiding. Dissecting and collecting liver, gills and blood samples.

• Practiced enzyme assay on plate reader (GSSG/GSH assay).

• Extensively worked on QPCR to analyze the gene expression of anti-oxidant and biotransformation enzymes in liver and gills of zebrafish.

• RNA Isolation (average 260/280 – 1.9, average 260/230 – 1.7).

• Experience of working with dry ice and liquid nitrogen.

• Practiced: Sterile Techniques, Preparation of Samples, Isolation of Samples, DNA, RNA, buffer preparation, Isolation of DNA, ICPMS analysis on fish samples, Documentation of Research utilizing MS Word, MS Excel. Academic Thesis Project:

Completed Master’s thesis on genetic toxicology in zebra fish. It is well established in mammals that thyroid hormone is essential for anti-oxidant production. Any thyroid modulating compound could lead to misbalance in the anti-oxidant response at any level from genes to proteins. Investigated this phenomenon in zebrafish to see if thyroid modulation could lead to any alterations in gene expression of anti-oxidant and biotransformation genes. Won competitive research grant for this project from graduate school.

Teaching Assistant

• Helped in designing the experiments.

• Instructed and supervised class of 20 students.

• Maintained good relations with all the students by motivating them when required, encouraged them for participation, rectified their mistakes by grading their lab reports.

COURSES and LABS

Analysis of environmental contaminants, Environmental Policy, Environmental Sampling, Toxicology, Molecular Toxicology, Statistics (SPSS), seminar courses. ENVIRONMENTAL PROTECTION TRAINING AND RESEARCH INSTITUTE Hyderabad, AP, India 2009-2009

Internship: Bio-monitoring of Musi river.

AWARDS

• Received full Research Grant for Graduate Student (RGGS) in fall 2012 for thesis project.

• Won second prize for poster presentation on CVM Research Day 2016. PUBLICATIONS

RL Carr, Nathan A, Alenda TB, Mohammed AN, Ross MK, Neil C 2015. Decreased anxiety in juvenile rats following exposure to low level of chlorpyrifos during developmen Neurotoxicology (In press).

Robert L Hybart, Navatha Alugubelly, Rachel Hybart, Afzaal N Mohammed, Carol Nail, Gretta parker, Mathew K Ross, and Russell Carr: Inhibition of endocannabinoid metabolism enzymes in peripheral tissue following developmental chlorpyriros exposure. (Submitted). Afzaal N Mohammed, Navatha Alugubelly, Barbara L Kaplan, and Russell Carr: Behavioral alterations in adolescent rats exposed to Tetrahydrocannabidiol as neonates: A childhood exposure scenario. (Submitted).

CONFERENCES

AN Mohammed, NH Amstrong, AT Buchnan, JB Eells, CA Nail, MK Ross, and RL Carr: Altered emotional reactivity and dopamine turnover in juvenile rats exposed developmentally to chlorpyrifos. Society of Toxicology: South central regional chapter meeting 2014.

Afzaal N Mohammed, Satya N Nagam, Chris W Theodorakis. Effects of thyroid hormones and arsenite of the expression of glutathione-metabolizing genes in zebrafish. Society of Environmental Toxicology and Chemistry 2014.

AN Mohammed, NH Amstrong, AT Buchnan, JB Eells, CA Nail, MK Ross, and RL Carr: Altered emotional reactivity and dopamine turnover in juvenile rats exposed developmentally to chlorpyrifos. Society of Toxicology annual meeting San Diego 2015. Afzaal N. Mohammed, Navatha Alugubelly, Barbara L Kaplan, and Russell Carr: Behavioral response in rats exposed to Tetrahydrocannabinol during neonatal ages. Society of Toxicology annual meeting New Orleans 2016.

Navatha Alugubelly, Afzaal N. Mohammed, and Russell L. Carr. Effect of repeated juvenile exposure to Permethrin and Deltamethrin on performance in adolescent rats on elevated plus maze. Society of Toxicology annual meeting New Orleans 2016.

Greta C. Parker, Navatha Alugubelly, Afzaal N. Mohammed, and Russell L. Carr. Effect of repeated juvenile exposure to Permethrin and Deltamethrin on social behavior in adolescent rats. Society of Toxicology annual meeting New Orleans 2016 Rachel L Hybart, Afzaal N. Mohammed Navatha Alugubelly, Barbara L Kaplan and Russell L. Carr.Effect of repeated juvenile exposure to Delta9-Tetrahydrocannbinol on social behavior adolescent rats on elevated plus maze. Society of Toxicology annual meeting New Orleans 2016.

Navatha Alugubelly, Mariola J Edelman, Afzaal N Mohammed, Bindu Nanduri and Russell Carr: Altered Neuroproteins in adolescent rats induced by early developmental exposure to chlorpyrifos. Society of Toxicology annual meeting Baltimore 2017. Rachel L Hybart, Navatha Alugubelly, Afzaal N Mohammed, and Russell Carr: Effect of repeated developmental exposure to low levels of Chlorpyrifos on biomarkers of exposure in the blood of juvenile rats. Society of Toxicology annual meeting Baltimore 2017.

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