Project Research

Maurizio Mauro, PhD Phone: 401-***-****

**** *********** ****** *** ** Emails: ********.*****@********.**.***

Bronx,NY, 10461 ********.******@*****.***

OBJECTIVE

To obtain a full time, PhD level position in biotech/ pharmaceutical company, utilizing my experience and skills in cell and molecular biology, to achieve advancement and growth for the company and myself.

SUMMARY OF QUALIFICATIONS

Driven and highly motivated scientist with in-depth knowledge of several cell biology areas including DNA damage response and repair using human/murine cancer and primary cell culture, with a productive 13-years track publications record;

Excellent collaborative skills as demonstrated by 6 successful collaborations;

Significant experience introducing new technologies and assays in laboratories;

Ability to lead and contribute to several parallel projects with strict time and budget constraints;

Paper/Grant writing and editing experience, as demonstrated by published articles;

Ability to successfully organize and plan experiments independently;

Teaching and leadership experience supervising several undergraduate/graduate students;

Ability to successfully present scientific projects to a diverse audience;

EDUCATION

University of Palermo Palermo, Italy

PhD in “Cell and Molecular Biology” March 2009

Thesis Title: " Role of epigenetic changes in the initiation and propagation of genomic instability”

University of Palermo Palermo, Italy

MSc. in Biology December 2001

Thesis Title: " Genomic instability induced by Limonene in mammalian cells ”.

RESEARCH EXPERIENCE

Albert Einstein College of Medicine

Prof. Zev Williams New York, NY

Senior Research Fellow February 2013 – to date

Performed, for the first time in the lab, DNA damage analysis in trophoblast cells using various techniques (Immunofluorescence, Western Blot and Comet Assay, ELISA);

Examined potential detrimental effects of LINE-1 retrotransposon on DNA integrity using next generation DNA/RNA sequencing, cell biology and biochemistry analysis in women with recurrent unexplained miscarriages. Data from this project are currently submitted to The New England journal of Medicine

Analyzing the potential use of reverse transcriptase inhibitors in combination with DNA repair inhibitors to treat cancer characterized by high LINE1 expression. Data from this project are currently submitted to Oncogene

Mentored 3 MD fellows in long term projects

University of Rhode Island Providence, RI

Prof. Niall Howlett December 2009 - January 2013

Research Fellow

Examined the role of p21 in DNA repair. Data from this project were published in NAR

Analyzed the regulation of the Fanconi anemia pathway by an ubiquitin-binding domain in the FANCD2 protein. Data from this project were published in Blood, Plos One, and In preparation for resubmission in Oncotarget

Mentored 8 undergraduate students on 5 different short term projects;

New York University New York, NY

Prof. Cathy Klein March 2008 – November 2008

Visiting researcher

Analyzed epigenetic changes induced by Arsenic in mammalian cells. Data from this project were published in Environ. Mol. Mutagen.

British American Tobacco Naples, Italy

Fellow June 2004 – December 2005

Applications of new technology, nanomaterial and biotechnology to research on tobacco products

University of Palermo Palermo, Italy

Prof. F. Caradonna June 2002 – May 2004

Fellow

Studied the genomic instability induced by Arsenic in mammalian models. Data from this project were published in Carcinogenesis, Environ. Mol. Mutagen, Toxicol In Vitro, Mutagenesis.

TECHNICAL SKILLS

Isolation and culture of mouse and human primary cells

Cell culture (primary and cancer cells)

Isolation of RNA and analysis by transcriptional profiling and quantitative PCR

Isolation of DNA and analysis by PCR and library preparation for next generation DNA sequencing

Western, Southern and Northern blotting

Fluorescence microscopy

In vitro assays for xenobiotic toxicity

Flow cytometry

DNA methylation analysis by pyrosequencing and Methylation Specific-PCR

Immunoprecipitation of complexes for identification by Mass Spectrometry

Cloning

Gene silencing by si/shRNAs. CRISPR

Cytogenetics techniques (G-banding, multicolor FISH)

PUBLICATIONS

Peer-Reviewed papers:

1.Vierra D., Rego M Garzon J, Mauro M, and Howlett N. Modulation of the Fanconi anemia pathway via chemically induced changes in chromatin structure. In preparation for resubmission in Oncotarget, 2017.

2.Andriani GA, Almeida VP, Faggioli P, Mauro M, Tsai WL, Santambrogio L, Maslov A, Gadina M, Campisi J, Vijg J, and Montagna C. Whole Chromosome Instability induces senescence and promotes SASP. Sci. Rep. Oct 12;6:35218. 2016.

3.Caradonnna F, and Mauro M. Role of the antioxidant defense system and telomerase in arsenic-induced genomic instability. Mutagenesis, 2016 Jul 28. pii: gew034.

4.Mauro M, Caradonna F, Klein CB. Dysregulation of DNA methylation induced by past arsenic treatment causes persistent genomic instability in mammalian cells. Environ. Mol. Mutagen. 2016 Mar;57(2):137-50. 2015.

5.Xin Li, Iddo Z Ben-Dov, Maurizio Mauro, Zev Williams. Lowering the quantification limit of the QubitTM RNA HS Assay using RNA spike-in. BMC Mol Biol. 16: 9. 2015.

6.Li X., Mauro M, and Williams Z. Comparison of plasma extracellular RNA isolation kits reveals kit-dependent biases. BioTechniques. Jul 1;59(1):13-7 2015.

7.Boisvert RA, Rego MA, Azzinaro PA, Mauro M, Howlett NG. Coordinate nuclear targeting of the FANCD2 and FANCI proteins via a FANCD2 nuclear localization signal. PLoS One. 2013 Nov 21;8(11):e81387

8.Mauro M, Catanzaro I, Naselli F, Sciandrello G, Caradonna F. Abnormal mitotic spindle assembly and cytokinesis induced by D-Limonene in cultured mammalian cells. Mutagenesis. 2013 2013 Nov;28(6):631-5. doi: 10.1093/mutage/get040.

9.Rego MA, Kolling FW 4th, Vuono EA, Mauro M, Howlett NG. Regulation of the Fanconi anemia pathway by a CUE ubiquitin-binding domain in the FANCD2 protein. Blood. 2012 Sep 6;120(10)

10.Mauro M, Rego MA, Boisvert RA, Esashi F, Cavallo F, Jasin M, Howlett NG. p21 promotes error-free replication-coupled DNA double-strand break repair. Nucleic Acids Res. 2012 Sep 1;40(17):8348-60.

11.Catanzaro I, Caradonna F, Barbata G, Saverini M, Mauro M, Sciandrello G. Genomic instability induced by α-pinene in Chinese hamster cell line. Mutagenesis. 2012 Jul;27(4):463-9.

12.MA Rego, JA Harney, M Mauro, M Shen and NG Howlett. Regulation of the activation of the Fanconi anemia pathway by the p21 cyclin-dependent kinase inhibitor. Oncogene. 2012 Jan 19;31(3):366-75.

13.Sciandrello G., Mauro M., Catanzaro I., Saverini M., Caradonna F. and Barbata G. Long-lasting genomic instability following arsenite exposure in mammalian cells: the role of reactive oxygen species. Environ Mol Mutagen. 2011 Apr 25.

14.Sciandrello G, Mauro M, Caradonna F, Catanzaro I, Saverini M, Barbata G. Acrylamide catalytically inhibits topoisimerase II in V79 cells. Toxicol In Vitro, 2010 24(3):830-4.

15.Caradonna F., Sciandrello G., Mauro M., Catanzaro I., Lacagnina P.K., Bellavia D. Rheumatoid arthritis-associated HLA-DRB1 genotypes in Western Sicily. Biologi italiani, 2007, vol. Aprile, pp. 42-44 ISSN: 0329-2510.

16.Sciandrello G., Caradonna F., Mauro M. and Barbata G. Arsenic-induced DNA hypomethylation affects chromosomal instability in mammalian cells. Carcinogenesis, 2004 (25) 413-41

BOOK

Panta rei: La genetica si racconta. Aracne editor (2007). ISBN: 978-88-548-0971-0

AWARDS

Student and New Investigator Travel Awards- EMS 39th Annual Meeting. Puerto Rico. October 2008.

Best poster: Antagonist effects of Acrylammide on clastogenicity of VP16. Kos, 09/3-7/2005.

TEACHING EXPERIENCE

2007-2009 University of Palermo: Courses of Genetics, Laboratory of Genetics, Environmental Mutagenesis

2008: New York University (NYU): Class of Cytogenetics and Epigenetics

INVITED SPEAKER

URI annual meeting: “ Role of p21 in DNA Repair”. Providence (RI) 11/2012.

EMS 39th Annual Meeting: “Long term exposure to submicromolar Arsenite induces chromosome instability via bypass of the spindle assembly checkpoint in mammalian cells”. Puerto Rico 10/18-22/2008.

36th Annual Meeting of the European Environmental Mutagen Society: “Persistent genomic instability by arsenic exposure in V79 cells”. Prague 07/2-6/2006.

9th International Symposium on Metal Ions in Biology and Medicine (9 ISMIBM): “Early and late effects of Arsenic exposure in mammalian cells”. Lisbon 05/21-24/2005.

PROFESSIONAL ASSOCIATION

Environmental Mutagen Society-USA. (Member)

European Environmental Mutagen Society. (Member)

Italian Biology Association. (Member)

Contact this candidate