ANA CATALINA DALESSIO, PH.D

* ********* ****, *********, ************* 02467

919-***-**** aczv7u@r.postjobfree.com

U.S. Citizen

PROFESSIONAL HIGHLIGHTS

Motivated and accomplished biomedical Ph.D. scientist focused on understanding the mechanisms controlling cell identity in

development of disease as team leader, team-member, or individual contributor. Skills include:

Extensive experience in genomics research.

Designed and implemented projects using disease modeling with iPSC.

Master theory and technical skills in molecular biology and CRISPR technology.

Develop complex and challenging projects, excel in tight deadlines.

Excellent verbal and written communication skills.

Goal to assist discovery and development of novel compounds for therapeutic use.

EMPLOYMENT/EDUCATION/TRAINING

Senior Scientist, FULCRUM THERAPEUTICS, Cambridge, MA 2016

Postdoctoral Associate, WHITEHEAD INSTITUTE FOR BIOMEDICAL RESEARCH, Cambridge, MA 2012 2015

Postdoctoral Associate, HOWARD HUGHES MEDICAL INSTITUTE. 2008 2011

Lineberger Comprehensive Cancer Center, the University of North Carolina at Chapel Hill, NC

Ph.D. Student, MCGILL UNIVERSITY, Laboratory of Dr. Moshe Szyf, Quebec, Canada. 1999 2007

B.Sc./M.Sc, School of Exact and Natural Sciences, UNIVERSITY OF BUENOS AIRES (UBA), Buenos Aires, Argentina. 1992 1999

TECHNICAL QUALIFICATIONS

Tissue Culture: Culture of human stem cells, human primary cells and cancer cell lines, lentivirus production and

cellular reprograming.

Molecular Biology: CRISPR technology, Southern blot, cloning and primer design, bacteria transformation and

mammalian cell transfections.

Genomics: ChIP-Seq, Medip-Seq, Bisulfite sequencing, chromosome confirmation capture, and genome-

wide chromatin occupancy analysis.

Biochemistry: Genetic Reporter assays, ELISA, dot and western blotting, protein production in bacteria, protein

purification, EMSA/gel shifts, In vitro assays to determine protein activity and antibody

production and purification.

Functional Cellular Assays: Immunostaining, RNAi.

PROFESSIONAL EXPERIENCE

FULCRUM THERAPEUTICS (a Third Rock Ventures Portfolio Company), Cambridge, MA 2016

Senior Scientist

Established disease model of Fragile X syndrome (FXS) using iPSC cells and iPSC derived glutamatergic neurons.

Led target validation efforts on the Fragile X syndrome disease area leading to the first validated target for the

company.

A N A C A T A L I N A D A L E S S I O, P H D Page 2

WHITEHEAD INSTITUTE FOR BIOMEDICAL RESEARCH, Cambridge, MA 2012-2015

Postdoctoral Associate, Laboratory of Dr. Richard A. Young

Led team that developed a novel genomics/computational method to predict master transcription factors.

Coordinated collaboration between three different laboratories.

Predicted master transcription factors in over 200 tissues/cell types.

Successfully validated computational method by reprogramming retinal pigment epithelial cells (RPE) from fibroblasts

using RPE predicted master transcription factors.

HOWARD HUGHES MEDICAL INSTITUTE, LINEBERGER COMPREHENSIVE CANCER CENTER, Chapel Hill, NC 2008 2011

Postdoctoral Associate, Laboratory of Dr. Yi Zhang, University of North Carolina at Chapel Hill

Team member pioneering the field of study of 5hmC and Tet proteins in mammalian cells.

Published work establishing 5hmC as a first step in DNA demethylation in high impact journals.

PUBLICATIONS

1. DAlessio AC*, Fan ZP*, Wert KJ, Cohen MA, Saini JS, Cohick E, Charniga C, DadonD, Hannett NM, Temple S, Jaenisch R,

Lee TI, Young RA. A Systematic Approach to Identify Candidate Transcription Factors that Control Cell Identity. Stem Cell

Reports. 2015 Nov 10;5(5):763-75. *Contributed equally to this work.

2. Nakagawa T, Lv L, Nakagawa M, Yu Y, Yu C, D'Alessio AC, Nakayama K, Fan HY, Chen X, Xiong Y. CRL4(VprBP) E3 ligase

promotes monoubiquitylation and chromatin inding of TET dioxygenases. Mol Cell. 2015 Jan 22;57(2):247-60.

3. Cassady JP, D'Alessio AC, Sarkar S, Dani VS, Fan ZP, Ganz K, Roessler R, Sur M, Young RA, Jaenisch R. Direct lineage

conversion of adult mouse liver cells and B lymphocytes to neural stem cells. Stem Cell Reports. 2014 Dec 9;3(6):948-56.

4. Theunissen TW, Powell BE, Wang H, Mitalipova M, Faddah DA, Reddy J, Fan ZP, Maetzel D, Ganz K, Shi L, Lungjangwa T,

Imsoonthornruksa S, Stelzer Y, Rangarajan S, D'Alessio A, Zhang J, Gao Q, Dawlaty MM, Young RA, Gray NS, Jaenisch R.

Systematic identification of culture conditions for induction and maintenance nave human pluripotency. Cell Stem Cell.

2014 Oct 2;15(4):471-87.

5. Li X, Wei W, Zhao QY, Widagdo J, Baker-Andresen D, Flavell CR, D'Alessio A, Zhang Y, Bredy TW. Neocortical Tet3-

mediated accumulation of 5-hydroxymethylcytosine promotes rapid behavioral adaptation. Proc Natl Acad Sci U S A.

2014 May 13;111(19):7120-5

6. Cheng J, Guo S, Chen S, Mastriano SJ, Liu C, D'Alessio AC, Hysolli E, Guo Y, Yao H, Megyola CM, Li D, Liu J, Pan W, Roden

CA, Zhou XL, Heydari K, Chen J, Park IH, Ding Y, Zhang Y, Lu J. An extensive network of TET2-targeting MicroRNAs regulates

malignant hematopoiesis. Cell Rep. 2013 Oct 31;5(2):471-81.

7. Chaffer CL, Marjanovic ND, Lee T, Bell G, Kleer CG, Reinhardt F, D'Alessio AC, Young RA, Weinberg RA. Poised chromatin at

the ZEB1 promoter enables breast cancer cell plasticity and enhances tumorigenicity. Cell. 2013 Jul 3;154(1):61-74.

8. Shen L, Wu H, Diep D, Yamaguchi S, D'Alessio AC, Fung HL, Zhang K, Zhang Y. Genome-wide analysis reveals TET- and TDG-

dependent 5-methylcytosine oxidation dynamics. Cell. 2013 Apr 25;153(3):692-706.

9. Kuzmichev AN, Kim SK, D'Alessio AC, Chenoweth JG, Wittko IM, Campanati L, McKay RD.Sox2 acts through Sox21 to

regulate transcription in pluripotent and differentiated cells. Curr Biol. 2012 Sep 25;22(18):1705-10.

10. Wu H*, DAlessio AC*, Ito S, Xia K, Wang Z, Cui K, Zhao K, Eve Sun Y, Zhang Y. Dual functions of Tet1 in transcriptional

regulation in mouse embryonic stem cells Nature. 2011 May 19;473(7347):389-93. *Equal contribution.

11. Wu H, DAlessio AC, Ito S, Wang Z, Cui K, Zhao K, Sun YE, Zhang Y. Genome-wide analysis of 5-hydroxymethylcytosine

distribution reveals its dual function in transcriptional regulation in mouse embryonic stem cells. Genes and

Development. 2011 Apr 1;25(7):679-84.

12. Ito S, DAlessio AC, Taranova OV, Hong K, Sowers LC, Zhang Y. Role of Tet proteins in 5mC to 5hmC conversion, ES-cell

self-renewal and inner cell mass specification. Nature. 2010 Aug 26;466 (7310):1129-33.

A N A C A T A L I N A D A L E S S I O, P H D Page 3

13. McGowan PO, Sasaki A, DAlessio AC, Dymov S, Labont B, Szyf M, Turecki G, Meaney MJ.Epigenetic regulation of the

glucocorticoid receptor in human brain associates with childhood abuse. Nature Neuroscience. 2009 Mar;12(3):342-8.

14. Tateishi K, He J, Taranova O, Liang G, DAlessio AC, Zhang Y. Generation of insulin-secreting islet-like clusters from human

skin fibroblasts. J Biol Chem. 2008 Nov 14;283(46):31601-7

15. Lamba P, Khivansara V, DAlessio AC, Santos MM, Bernard DJ Endocrinology. Paired-like homeodomain transcription

factors 1 and 2 regulate follicle-stimulating hormone beta-subunit transcription through a conserved cis-element. 2008

Jun;149(6):3095-108.

16. DAlessio AC, Weaver IC, Szyf M. Acetylation induced transcription is required for active DNA demethylation in

methylation silenced genes. Mol Cell Biol. 2007 Nov;27(21):7462-74

17. Caporaletti D, DAlessio AC, Rodriguez-Suarez RJ, Senn AM, Duek PD, Wolosiuk RA. Non-reductive modulation of

chloroplast fructose-1,6-bisphosphatase by 2-Cys peroxiredoxin. Biochem Biophys Res Commun. 2007 Apr 13;355(3):722-

7.

18. Weaver IC, DAlessio AC, Brown SE, Hellstrom IC, Dymov S, Sharma S, Szyf M, Meaney MJ. The transcription factor NGFI-A

mediates epigenetic programming by maternal behavior. J Neurosci. 2007 Feb 14;27(7):1756-68

19. Milutinovic S*, DAlessio AC*, Detich N, Szyf M. Valproate induces widespread epigenetic reprogramming which involves

demethylation of specific genes Carcinogenesis 2007 Mar;28(3):560-71 *Equal contribution.

20. DAlessio AC, Szyf M. Epigenetic tte- -tte. Biochem Cell Biol. 2006 Aug;84(4):463-76. Review.

21. Weaver IC, Cervoni N, Champagne FA, DAlessio AC, Sharma S, Seckl JR, Dymov S, Szyf M, Meaney MJ. Epigenetic

programming by maternal behavior. Nature Neuroscience. 2004 Aug;7(8):847-54

HONORS AND AWARDS

Undergraduate Fellowship, UNIVERSITY OF BUENOS AIRES, Argentina 1995-1997

Junior Research Fellowship, UNIVERSITY OF BUENOS AIRES, Argentina 1997-1999

Deans Honor List for B.Sc., UNIVERSITY OF BUENOS AIRES, Argentina 1999

Fellowship to Study Abroad, National Research Council of Argentina (C.O.N.I.C.E.T) 1999-2000

School of Medicine Internal PhD fellowship. MCGILL UNIVERSITY, Montreal, Canada 2002-2004

School of Medicine Internal PhD fellowship. MCGILL UNIVERSITY, Montreal, Canada 2005-2006

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