Senior Research Fellow

Chayan Roy

Senior Research Fellow

DOB: **th June, 1987

Rathindra Palli (North)

Sriniketan, Birbhum,

West Bengal, India, Pin-731236

Email – ******.*****@*****.***

********@******.**.**

Mob – +91-947*******

Career goals: To continue research and development in the field of microbial ecology, bioinformatics, comparative genomics and metagenomics. In addition studying complex microbial ecology and evolution, I am always eager to learn new techniques.

Academic qualifications:

Research experience:

November 2011- till date: Working as a Ph.D. student in Dept. of Microbiology, Bose Institute, Kolkata. Thesis title “Thermophilic adaptation of Phylogenetically Diverse Bacterial Groups”.

March 2011-November 2011: Worked as a project assistant at NIPGR, New Delhi, under Dr. Ananda K. Sarkar on Plant Molecular Genetics on: “Role of plant miRNA and tasiRNA in plant root development and especially on lateral root emergence and maintenance.” Examination Year University Main Subjects Marks Ph.D. 2011- Calcutta

University Environmental Microbiology

M.Sc. 2010 Visva-Bharati Botany with Applied &

Fundamental Microbiology 844 (70.33)

B.Sc. 2008 Visva-Bharati Botany (Hons.) 659

(65.9)

Pre degree (XII) 2005 Visva-Bharati Chem.,Lif., Phy., Math. 800 (72.72) School final (X) 2003 Visva-Bharati Phy.Sc. Lif. Sc. Hist. Geo. Math. 722 (80.22)

Technical expertise:

Fundamental Microbiology- Culture and maintenance of several bacterial inoculations, Isolation and purification of several types of bacteria, study on air mycoflora, Bacterial growth curve and Antibiotic sensitivity test.

Molecular Biology- Plasmid, Genomic DNA, Cellular RNA isolation, PCR, Restriction Digestion, Molecular Cloning.

Advanced Techniques- Whole cell protein purification (Phenol-Chloroform based), 2-D gel electrophoresis and MALDI-MS, DNA Sequencing (Capillary and Next Generation Sequencing). SDS-PAGE & Agarose gel electrophoresis, Paper and column chromatography, Laser Scanning Confocal Microscopy. Computational skills- High through put NGS data analysis, assembly of bacterial draft genome with MIRA, Newbler, SOAPdenovo, ABySS etc. assembler and further downstream genome finishing with GAP, HAF tools, Cytoscape etc. Construction of population genome bins using GroupM, METABat etc. OTU clustering with QIIME, USEARCH, ESPRIT etc.. Working knowledge of MS Office, Adobe Photoshop CS-6, Corel Draw, MEGA, GENEIOUS, Bio-edit, ESPRIPT, packages of “R”, basics of Perl etc.

Summary According of doctoral to the work first mandate and research of my interest work full range of microbial diversity associated with the geothermal vents of various regions of India was explored by high- throughput culture-independent techniques. Metagenomic analysis showed that surprisingly these high temp. habitats dominated by phylogenetic relatives of mesophilic bacteria and further downstream analysis also revealed that presence of terminal alanine in the pentapeptide precursor of bacterial peptidoglycan makes them susceptible to the antibiotic Vancomycin (specific for gram positive bacteria) irrespective of their gram character. Two other separate studies was conducted to explore and analyze the microbial mats from Puga geothermal area of Laddakh and cluster of hot springs located near Tapovan in Uttarakhand, India. Comparative metagenomic analysis of the first microbiome was done to elucidate the community dynamics, or trends of dispersion (thermal amplitude / niche width) and consolidation (niche preference/niche specializations) of bacterial populations. While the second study was performed to reveal the different niche opportunities and constraints that govern the stability and sustainability of geothermal microbiota over spatiotemporally fluctuating geochemical conditions. Beside these in a collaborative project with the National Institute of Oceanography

(NIO), Goa (a CSIR institute, Govt. of India), I am working on the metagenomic data of Oxygen Minimum Zone (OMZ) sediments and water samples. The special focus is to find the role of methane and different sulfur compounds in microbial ecology of OMZ of Arabian Sea.

In addition to that I have participated in whole genome projects of various bacteria and archaea.

Analyzing microbial ecology of several extreme habitats and different bio- geochemical cycles, with the emphasis on the role of microbes is my foremost interest. In this regard I would like to work on the metatranscriptomics as well as metaproteomics high throughput data along with metagenomics to explore the microbial ecology of a particular habitat.

Chayan International Roy, Masrure Publications Alam, Subhrangshu Mandal, Prabir Kumar Haldar et al. Global association between thermophilicity and vancomycin susceptibility in bacteria Frontiers in Microbiology, March 2016, Volume7, Wriddhiman Article Ghosh, 412. Chayan Roy, Rimi Roy, Pravin Nilawe et al. Resilience and receptivity worked in tandem to sustain a geothermal mat community amidst erratic environmental conditions Scientific Reports, 5, Sucharita 12179 (2015)Bose, . Trinetra Mukherjee, Urmimala Sen, Chayan Roy et al. Genome Sequence of multiple protease producing Geobacillus thermoleovorans strain N7, a thermophilic bacterium, isolated from Paniphala hot spring, West Bengal, India Genome Announcements, 2016 October 27. 4(5):e01202-16. doi:Urmimala 10.1128, Sen, 2016)Trinetra . Mukherjee, Sucharita Bose, Chayan Roy et al. Genome sequence of an arsenic resistant Haladaptatus sp. strain R4 isolated from Ramnagar, West Bengal, India Genome Announcements, 2016 Trinetra Sep Mukherjee, 22;4(5). pii: Sucharita e01017-16. Bose, Urmimala Sen, Chayan Roy et al. Genome sequence of the red pigment forming Meiothermus taiwanensis strain RP isolated from Paniphala hot spring, India Genome Announcements Wriddhiman Ghosh, (2016) Masrure 4(3): e00629-Alam, 16. Chayan Roy, Prosenjit Pyne et al. Genome Implosion Elicits Host- Confinement in Alcaligenaceae: Evidence from the Comparative Genomics of Tetrathiobacter kashmirensis, Masrure Alam, a Pathogen Chayan Roy, in the Prosenjit Making Plos Pyne, One, Atima May Agarwal 2013, Volume et al. Whole-8, Issue Genome 5, e64856. Shotgun Sequence of the Sulfur-Oxidizing Chemoautotroph Pseudaminobacter salicylatoxidans KCT001 Journal of bacteriology September 2012, Volume 194, Number 17, p- 4743–4744.

Conferences November 12-attended: 14 2014- 55th annual conference of Ass. Of Microbio. of India on “National Conference on Empowering Mankind with Microbial Technologies” at Tamil Nadu Agricultural University, October Coimbatore, 17th, Tamil 2016- Nadu, National India. symposium organized by Bose Institute, Kolkata, India on “The World of Microbes, Pathogenesis, Environment and Evolution”.

Awards Dec 2010, and National fellowships: entrance test for Assistant Professor and doctoral research fellowship (CSIR- UGC-NET). Nov 2011-Nov 2013- Junior research fellowship, Nov 2013-Nov 2016- Senior research fellowship

IIT-M GATE (Graduate Aptitude Test in Engineering), 2010-11.

Referees- RET (Research Entrance Test) 2010-2011 from Visva-Bharati University, West Bengal, India. I, hereby declare that the above-furnished particulars are true to the best of my knowledge and belief. If given a chance, I will prove my efficiency and willingness to work. Kolkata, 08/12/2016 CHAYAN ROY

Dr. Wriddhiman Ghosh

(Ph.D. mentor)

Microbiology, Bose institute

P1/12 CIT road scheme VIIM

Kolkata- 700054

Email: ******@******.**.**

Prof. Sujoy k. Das Gupta

Microbiology, Bose institute

P1/12 CIT road scheme VIIM

Kolkata- 700054

Email: *****@******.**.**

Dr. Bomba Dam

Department of Botany

Siksha Bhavana, Visva-Bharati

Santiniketan - 731235

Email: ***********@**********.***

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