Medical Human

Ye Lu

**** ****** ***,

Ann Arbor, MI *****

E-mail: ******@*****.***

Tel: 734-***-**** (cell)

Qualifications / Achievements

Experienced in designation and preformation of molecular biology experiments, such as PCR, RT-PCR & real-time PCR; Western blot, immunoprecipitation/Co-IP & ELISAs; DNA cloning, DNA/RNA extraction & electrophoresis, etc.

Experienced in histopathology analysis, flow cytometry, immunofluorescence and immunochemistry, and related techniques of tissue dissecting of paraffin and crystal.

Developed cancer cell lines; Induced human neurons from NT2 cell line; Experience in tissue culture including primary tissues, cell lines, yeasts and bacteria, and related techniques of cell activation, proliferation and apoptosis.

Created transgenic animals, developed cancer metastasis mouse models; Experience of mouse colonies maintenance, and related techniques of mouse surgeries, xenograft, genotypes, injections, necropsy, lavage, and nasal dosing etc.

Research Experience

1. Inhibitors of cell signaling pathway in T-cell lymphomas and macrophages associated

TCR engagement promotes resistance to chemotherapy in T-cell lymphomas in an ITK and GATA-3-dependent manner.

Investigated GATA-3 regulates IL-10 production and macrophage polarization in T-cell lymphomas

JAK2/FLT3 inhibitor depletes CLL-associated macrophages and impairs CLL cell viability.

Generated T-cell lymphoma mouse models using transgenic mice and xenografts.

Performed and supported projects at lab by real-time PCRs, Western blots, immunoprecipitation/Co-IP, and ELISAs;

2. Molecular mechanisms and therapeutic strategies of Alzheimer’s' disease

Recombined DNA of human myc-tagged X11α, Tau and APPswe with plasmid and cosmid vectors for transgenic animals and transformations of cell line, included enzymatic modifications, mutagenesis, transfections, expressions and immunoprecipitation.

Designated PCRs and applied for genotyping human myc-tagged X11α transgenic mice, APPswe/X11α, APPswe/Precilin1/X11α co-transgenic mice.

Performed Western blots, immunoprecipitations, RT-PCRs, ELISAs and immunochemistry stains for studying on interactions between the human X11α, Fe65 and APP in 293 cell, neuron induced from NT2 cell and transgenic animals.

Incorporated with Transgenic Animal Core, created human myc-tagged X11α (mint1) transgenic mice; Generated APPswe/X11α and APPswe/Precilin1/X11α co-transgenic mice; Constructed and screened transgenic rats of human Tau and APPswe.

3. Autoimmunity and LPLA2 (lysosomal phospholipase A2) deficiency

Genotyped and characterized LPLA2 deficiency mice, investigated into LPLA2/ leads increased autoimmunity associated with late onset Systemic Lupus Erythematosus.

Developed assays of immunofluorescence and immunochemistry for detecting IgG heavy chain, C1q and C3, and CD68 in LPLA2 / tissues.

Designated, performed and analyzed the clusters of differentiation, proliferation and apoptosis of T, B cells and macrophages in spleen, bone marrow, peripheral blood and alveolar lavage cells by flow cytometry and H3-thymidine.

Performed assays of cytokines, Anti-double-stranded DNA (dsDNA) autoantibodies,

circulating immune complexes, immunoglobulin isotopes in culture supernatant and serum.

4. Model development and therapeutics of cancer metastasis

Developed metastasis models of lymphatic and pulmonary in mouse breast cancer Ca761.

Established cell lines with different metastasis phonotypes from mouse breast cancer Ca761.

Investigated metastatic cancer organ specificities from differential metastatic models in vitro / vivo, and the interactions between metastatic cells and their host organ in molecular and cell physics.

Performed chemo resistance / sensitivity assays of metastasis cells of breast cancer by RT-PCR, MTT and H3-thymidine.

Employment History

1998-Present, Research Specialist Intermediate, University of Michigan

1991-1998, Pathologist, Dalian Municipal Central Hospital, China

1985-1988, Lecturer, Pathology, Yanan Medical School, China

Education

Master of Pathology, Dalian Medical University, 1988-1991

Degree of Medicine, Medical School, Yanan University, 1980-1985

Training

Confocal Microscopy, Leica Confocal Microscopy Course, May 2010

Basic and Multiparameter Flow Cytometry, Flow Regional Training Program, September 2007

Microinjection and Transgenic Animal, Transgenic Animal Core, U of M, June 2000

Basic Molecular Cloning, Medical Science Institute of China, July 1997

Recent Publications

1, Wang TJ, Lu Y, Polk A, and Wilcox RA et al: T-cell receptor signaling activates an ITK/NF-κB/GATA-3 axis in T-cell lymphoproliferative disorders facilitating resistance to chemotherapy. Clinical Cancer Research 2016, Oct 25

2, Polk A, Lu Y, Wang TJ, and Wilcox RA et al: Colony-stimulating Factor-1 Receptor is Required for Nurse-like Cell Survival in Chronic Lymphocytic Leukemia. Clinical Cancer Research 2016 Jun 22

3, Wang TJ, Feldman AL, Wada DA, Lu Y, Polk A, and Wilcox RA et al: GATA-3 expression identifies a high-risk subset of PTCL, NOS with distinct molecular and clinical features. Blood 2013 Nov 15,

4. Abe A, Kelly R, Kollmeyer J, Hiraoka M, Lu Ye and Shayman JA: The Secretion and Uptake of Lysosomal Phospholipase A2 by Alveolar Macrophages. The Journal of Immunology 2008; 181: 7873-7881.

5. Hiraoka M, Abe A, Lu Ye, Yang K, Han X, Gross R W, and Shayman JA: Lysosomal Phophlipase A2 and Phospholipdosis. Molecular and Cellular Biology 2006; 6139-6148

6. Corcoran KA, Lu Ye, Turner RS, and Maren S: Overexpression of hAPPswe Impairs Rewarded Alternation ans Contextual Fear conditioning in a Transgenic Mouse Model. Learning and Memory 2002; 9.5: 243-252

Conference Presentations

1. Heterogeneities of Metastatic Mammary Carcinomas and Organ Tendencies of Different Metastatic Models in Ca761. The 4th National Conference, Chinese Society of Cancer Immunotherapeutic Strategy 1995

2. Establishment and Characterization of Mammary Carcinoma Cell lines with Different Metastatic Phenotype in Ca761. The 2nd National Conference, Chinese Society of Cancer Metastasis 1992

Selected Abstracts

1. Lu Ye, Corcoran KA, King GD, Furay AR, Margolis B, Albin RL, Saunders TL, Maren S, Turner RS: In Vivo Modulation of APP Catabolism in Brain and Enhanced Learning and Memory in Human X11 Transgenic Mice. Neurobiol of Aging 2002; 23, S240

2. Lu Ye, Saunders TL, VanKeuren M.L., ZamanFT, Furay AR, Gan CC, Margolis B, Albin RL, Turner RS: Generation and Characterization of Novel Human X11/Mint1 Transgenic Mice. Michigan SFN, 2001; S43

3. Gau J-T, Desmond TJ, Austin LL, Lu Ye, Cherian K, Kao T-C, D'Amato CJ, Frey KA, Turner RS: Progressive CNS Amyloidogensis in Aging Human Amyloid Precursor Protein Swedish Mutation Transgenic Mice Does Not Induce Cholinergic Dysfunction. American Geriatric Society 2001; S2A

4. Saluja I, Lu Ye, Saunders T, Turner RS: Regulatory roles of X11/mint-1 in Neurons. Soc for Neurosci 2003; 29, 523.4

5. Turner RS, King GD, Saluja I, Lu Ye, Cherian K, Saunders TL: Regulation of amyloid precursor protein (APP) trafficking and processing by X11/mint-1 interaction. Neurology 2004; 62, A522

6, Wang TJ, Lu Y, Polk A, Chowdhury P, Wilcox RA: T-cell receptor signaling activates an ITK/NF-κB/GATA-3 axis in T-cell lymphoproliferative disorders facilitating resistance to chemotherapy. Blood 2015 Dec.3 126 (23)

Patent

Co-inventor, Lysosomal Phospholipase A2 Activity as a Diagnostic and Therapeutic Target for Identifying and Treating Systemic Lupus Erythematosis. USA patent number: 200********

References Available upon request

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