BINDIBEN

PATEL

A biotech professional with extensive molecular biology, cell biology and biochemistry technical expertise. Proficient in various cell-based assays and invitro and invivo techniques involving different cell types as well as mice surgeries. Extensive experience with advanced imaging techniques like TEM, IHC, IF etc. Well versed with writing scientific manuscripts, protocols and drafted experimental outcomes. Hillsborough, NJ 08844 aczsp2@r.postjobfree.com 732-***-**** SUMMARY OF

QUALIFICATIONS

§ 7 years of experience in assisting research studies in a high paced laboratory setting requiring various technical expertise and meet publication deadlines.

§ Developing various cell-based assays involving molecular/genetic targeting, protein biochemistry and fluorescent reporter tools.

§ Trouble-shooting and optimization of various techniques depending on the type of study and resources available.

§ Good record of publications implying vast areas of research study samples and goals. EDUCATION

M.S. BIOTECHNOLOGY New York University Tandon School of Engineering 2009 GPA 3.9/4.0

B. PHARMACY University of Pune, India

2007 GPA 3.8/4.0

CERTIFICATIONS

ELECTRON MICROSCOPY TECHNOLOGIST

Cert # 2084 MSA-CEMT (2015); Microscopy Society of America, USA CRYOMETHODS, ULTRAMICROTOMY & IMMUNOLABELLING

Certified Workshop(2010); CMC, University Medical Center Utretch, Netherlands PROFESSIONAL

EXPERIENCE

RESEARCH SPECIALIST II PRINCETON UNIVERSITY

August 2016 – Present Princeton, NJ

Dr. Alexander Nectow Laboratory

§ Developing molecular tools to elucidate the molecular and neural circuit mechanisms in neurodegenerative disorders. Models include neural cell lines and mice.

§ Approaches involve administration of Adeno-Associated Virus targeting different neuronal cell types into the mice brain by lateral/bilateral mice surgeries.

§ Manage day-to-day laboratory tasks and coordinate research experiments. OPERATIONS MANAGER ALBERT EINSTEIN COLLEGE OF MEDICINE August 2015 – July 2016 Bronx, NY

Institute of Aging Research, Proteostasis of Aging Core; Director: Dr. Nir Barzilai Provide state-of-the-art methodology for conducting analysis for cellular protein homeostasis and molecular pathways in models related to aging and related disorders.

§ Provide advice on experimental design, techniques, data interpretation; experimental analysis and service including specialized assays for analysis of autophagic pathways.

RESEARCH TECHNICIAN C ALBERT EINSTEIN COLLEGE OF MEDICINE July 2012 – July 2016 BRONX, NY

Dr. Ana Maria Cuervo Laboratory

§ Designed experiments to dissect developmental aspects in Neurodegenerative disorders like Parkinson’s Disease, Huntington;s Disease.

§ Elucidated functional consequences of altered protein degradation pathways in these disorders using various cellular/animal models.

§ Perform sophisticated microscopy techniques including TEM, Cryo-TEM, high- throughput screening assays.

RESEARCH TECHNICIAN B ALBERT EINSTEIN COLLEGE OF MEDICINE June 2009 – July 2012 Bronx, NY

Dr. Ana Maria Cuervo Laboratory

§ Studied developmental aspects of autophagic pathways in T-cell activation, Parkinson disease iPSC cellular models.

§ Specialized in TEM, Experience with latest advanced techniques on Cryomethods in TEM (Workshop certification in CryoImmunogold TEM) RESEARCH ASSISTANT VOLUNTEER SUNY DOWNSTATE

August 2008 – May 2009 New York, NY

Dr. Edward Quadros Laboratory

SKILLS AND

EXPERTISE

§ Molecular Biology/Biochemistry/Cell Biology: DNA isolation, RNA isolation, restriction and PCR cloning, real-time PCR (RT-PCR), qPCR, gene cloning, SDS- PAGE, IB, ELISA, Co-Immunoprecipitation, Organelle isolation using density gradient techniques, Ex-vivo organ isolations, Tissue/Cell culture, Gene-targeting, transfections and expression, Cell-based bioassays, Lenti-virus packaging and transduction using shRNA, RNA interference (RNAi), radioactivity based cell/organelle uptake-binding assays, Cell viability assays. Worked with multi-well plate assays (6-, 12-, 24-, 48-, 96-, 384- well plates) for high-throughput outcomes.

§ Imaging Techniques: Immunohistochemistry (IHC), RNA-FISH, DNA-FISH, Dual IHC-FISH, Immunofluorescence (IF), High content microscopy, Morphometric analysis and quantifications, Cell-based fluorescent reporter assays.

§ In-vivo experimental skills: Stereotaxic brain surgeries / AAV-injections using surgery frames, IP injections, animal perfusions, and organ isolation/dissection: retina, muscle, liver, bone marrow, diaphragm and brain. Tissue slicing using vibratome, cryostat.

§ Transmission Electron Microscopy: Highly specialized in Preparing, Fixing, Processing, Embedding samples for Electron Microscopy along with post specimen preparations like sectioning, staining and analysis. TEM involved Morphological and Immunogold analysis of cell organelles, cells and tissue samples by Epon/Lowicryl/LR resin embedding, ultracryotomy, immunolabelling. Cryo-EM (Certification in CryoImmunogold TEM).

§ Managing Activities: Plan and perform experiments for assigned projects, interpret results, discuss and draw conclusions, managing group collaborations and various lab tasks, adaptable to fast-paced environment and ability to upskill rapidly.

§ Scientific Writing and Presentations: Writing scientific manuscripts of assigned projects. Quaternary laboratory meeting visual/verbal presentations. PUBLICATIONS &

PEER-REVIEWING

Please refer the addendum to this resume.

Addendum (BINDIBEN PATEL RESUME)

PUBLICATIONS (AWARDS AND RECOGNITIONS HIGHLIGHTED) 1. Maus M, Cuk M, Patel B, Lian J, Ouimet M Kaufmann U, Yang J, Horvath R, Hornig-Do, H, Chrzanowska- Lightowlers Z, Moore K, Cuervo AM, Feske S. “Store-Operated Ca2+ Entry Controls Induction of Lipolysis and the Transcriptional Reprogramming to Lipid Metabolism.” Cell Metabolism (2017) Mar 7;25(3):698-712. 2. Mukherjee A, Patel B, Koga H, Cuervo AM, Jenny A. “Selective endosomal microautophagy is starvation- inducible in Drosophila.” Autophagy (2016) Aug 3:1-16. 3. Mitchell SJ, Madrigal-Matute J, Scheibye-Knudsen M, Fang E, Aon M, González-Reyes JA, Cortassa S, Kaushik S, Gonzalez-Freire M, Patel B, vvvvvvvv Cuervo AM, Pearson KJ, Ingram DK, Bernier M, de Cabo R. “Effects of Sex, Strain, and Energy Intake on Hallmarks of Aging in Mice. ” Cell Metabolism (2016) Jun 14;23(6):1093-112.

4. Champa D, Orlacchio A, Patel B, Ranieri M, Shemetov AA, Verkhusha VV, Cuervo AM, Di Cristofano A.

“Obatoclax kills anaplastic thyroid cancer cells by inducing lysosome neutralization and necrosis.” Oncotarget

(2016) Vol. 7 No. 23.

5. Botbol Y, Patel B, Macian F. “Common γ-chain cytokine signaling is required for macroautophagy induction during CD4+ T cell activation.” Autophagy (2015) Oct 3;11(10):1864-77. 6. Arias E, Koga H, Diaz A, Mocholi E, Patel B, Cuervo AM. “Lysosomal mTORC2/PHLPP1/Akt Regulate Chaperone-Mediated Autophagy.” Molecular Cell (2015) June 24 S1097-2765(15)00397-4. 7. Rui NY, Xu Z, Patel B, Cuervo AM, Zhang S. “HTT/Huntingtin in Selective Autophagy and Huntington Disease: A foe or a friend within?” Autophagy (2015) May 4;11(5):858-60. 8. Rui NY*, Xu Z*, Patel B*, Chen Z, Chen D, Tito A, David G, Sun Y, Bellen H, Cuervo AM, Zhang S.

“Huntingtin functions as a Scaffold for Selective Macroautophagy.” Nature Cell Biology (2015) 17(3):262-75.

*Co-first author.

• News and Views: Huntingtin facilitates selective autophagy. Gelman A, Rawet-Slobodkin M, Elazar Z. Nature Cell Biology (2015) 17:214–215.

• Feature Editorial: Selective autophagy and Huntingtin: learning from disease. Cuervo AM, Zhang S. Cell Cycle (2015).

9. Schneider JL, Villarroya J, Diaz-Carretero A, Patel B, Urbanska AM, Thi MM, Villarroya F, Santambrogio L, Cuervo AM. “Loss of hepatic chaperone-mediated autophagy accelerates proteostasis failure in aging.” Aging Cell (2015) 14(2):249-64.

10. Patel B, Cuervo AM. “Tools and methods to study chaperone-mediated autophagy.” Methods (2015) Mar;75:133-40.

11. Bejarano E, Yuste A, Patel B, Spray D, Cuervo AM. Connexins modulate autophagosome biogenesis. Nature Cell Biology (2014) Published Online on April 6 2014, doi: 10.1038/ncb2934. 12. Pampliega O, Orhon I, Patel B, Sridhar S, Diaz-Carretero A, Beau I, Codogno P, Satir B, Satir P, Cuervo AM. Functional interaction between autophagy and ciliogenesis. Nature (2013) 502:194-200.

• News and Views: Cell Biology: Molecular clearance at the cell’s antenna. Cianfanelli V, Cecconi F. Nature

(2013) 502:180-181.

• Research Highlights: Autophagy and ciliogenesis come together. Wrighton KH. Nature Reviews in Molecular Cell Biology (2013) 14: 687.

• Preview: Cilia grow by taking a bite out of the cell. Pierce NW, Nachury MV. Developmental Cell (2013) 27:126-7.

• EMBO Meeting 2013 Travel Award: Recognized Conference Abstract and Poster; Flash Talk and Poster

(selected from the presented abstracts). EMBO meeting, September 2013, Amsterdam, The Netherlands. 13. Sridhar S, Patel B, Aphkhazava D, Macian F, Santambrogio L, Shields D and Cuervo A.M. The lipid kinase PI4KIII preserves lysosomal identity. EMBO Journal (2012) 32:324 - 339.

• Have You Seen: PIPing on lysosome tubes. Ktistakis NT, Tooze SA. EMBO Journal (2013) 32:315-317.

• Faculty 1000 Prime Biology Recommended: Balla T, Jovic M. (2013)

• EMBO meeting 2012 Travel Award: Recognized Conference Abstract and Poster. EMBO meeting, 2012, Nice, France.

14. Arduíno DM, Esteves AR, Cortes L, Silva DF, Patel B, Grazina M, Swerdlow RH, Oliveira CR, Cardoso SM. Mitochondrial metabolism in Parkinson's disease impairs quality control autophagy by hampering microtubule- dependent traffic. Human Molecular Genetics (2012) 21:4680-4702. 15. Bejarano E, Girao H, Yuste A, Patel B, Marques C, Spray DC, Pereira P, Cuervo AM. "Autophagy modulates dynamics of connexins at the plasma membrane in a ubiquitin-dependent manner." Molecular Biology of the Cell (2012) 23:2156–2169.

• Faculty 1000 Prime Biology Recommended: Bayer E (2012) 16. Sanchez-Danes A, Richaud-Patin Y, Carballo-Carbajal I, Jimenez-Delgado S, Caig C, Mora S, Di Guglielmo C, Ezquerra M, Patel B, Giralt A, Canals JM, Memo M, Alberch J, Lopez-Barneo J, Vila M, Cuervo AM, Tolosa E, Consiglio A, Raya A. "Disease-specific phenotypes in dopamine neurons from human iPS-based models of genetic and sporadic Parkinson's disease." EMBO Molecular Medicine (2012) 4:380–395. 17. Hubbard VM, Valdor R, Patel B, Singh R, Cuervo AM, Macian F. "Macroautophagy regulates energy metabolism during effector T cell activation." Journal of Immunology (2010) 185:7349-7357. SERVED AS PEER REVIEWER

§ “Degenerative Neurological and Neuromuscular Disease” Journal

§ “Neuropsychiatric and Treatment” Journal

§ “Research and Reports in Biology” Journal

§ “Open Journal of Biochemistry” Journal

GOOGLE SCHOLAR PROFILE FOR CITATIONS: Bindiben Patel, Princeton University

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